(Linear) (Six-base) MAPSORT of: KIBB3007.seq Check: 7969 from: 1 to: 3747 KIBB3007.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AccI GT'mk_AC Cuts at: 0 140 1102 1776 3747 Size: 140 962 674 1971 Fragments arranged by size: 1971 962 674 140 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1616 1684 1863 2039 2177 3086 3747 Size: 1616 68 179 176 138 909 661 Fragments arranged by size: 1616 909 661 179 176 138 68 AflII C'TTAA_G Cuts at: 0 289 1809 3747 Size: 289 1520 1938 Fragments arranged by size: 1938 1520 289 AflIII A'CryG_T Cuts at: 0 798 1791 3747 Size: 798 993 1956 Fragments arranged by size: 1956 993 798 AleI CACnn'nnGTG Cuts at: 0 1456 3747 Size: 1456 2291 AlwNI CAG_nnn'CTG Cuts at: 0 2893 3747 Size: 2893 854 ApaI G_GGCC'C Cuts at: 0 1257 3747 Size: 1257 2490 ApoI r'AATT_y Cuts at: 0 463 543 869 1008 1019 1725 1973 2113 Size: 463 80 326 139 11 706 248 140 Cuts at: 2113 2191 2551 3086 3598 3747 Size: 78 360 535 512 149 Fragments arranged by size: 706 535 512 463 360 326 248 149 140 139 80 78 11 AseI AT'TA_AT Cuts at: 0 1034 3747 Size: 1034 2713 AvaI C'yCGr_G Cuts at: 0 144 3747 Size: 144 3603 AvrII C'CTAG_G Cuts at: 0 2172 3747 Size: 2172 1575 BamHI G'GATC_C Cuts at: 0 1797 2911 3747 Size: 1797 1114 836 Fragments arranged by size: 1797 1114 836 BanII G_rGCy'C Cuts at: 0 26 153 789 1257 1443 3747 Size: 26 127 636 468 186 2304 Fragments arranged by size: 2304 636 468 186 127 26 BbsI GAAGACnn'nnnn_ Cuts at: 0 1220 1343 3301 3747 Size: 1220 123 1958 446 Fragments arranged by size: 1958 1220 446 123 BciVI GTATCCnnnnn_n' Cuts at: 0 377 3734 3747 Size: 377 3357 13 Fragments arranged by size: 3357 377 13 BfrBI ATG'CAT Cuts at: 0 1363 2296 3565 3747 Size: 1363 933 1269 182 Fragments arranged by size: 1363 1269 933 182 BglII A'GATC_T Cuts at: 0 2346 3078 3747 Size: 2346 732 669 Fragments arranged by size: 2346 732 669 Bme1580I G_kGCm'C Cuts at: 0 1257 3390 3747 Size: 1257 2133 357 Fragments arranged by size: 2133 1257 357 BmrI ACTGGGnnnn_n' Cuts at: 0 2385 2389 3747 Size: 2385 4 1358 Fragments arranged by size: 2385 1358 4 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 2662 3747 Size: 636 2026 1085 Fragments arranged by size: 2026 1085 636 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 476 672 1472 2772 3404 3747 Size: 358 118 196 800 1300 632 343 Fragments arranged by size: 1300 800 632 358 343 196 118 BsaI GGTCTCn'nnnn_ Cuts at: 0 46 52 3747 Size: 46 6 3695 Fragments arranged by size: 3695 46 6 BsaAI yAC'GTr Cuts at: 0 1792 3747 Size: 1792 1955 BsaBI GATnn'nnATC Cuts at: 0 2615 3747 Size: 2615 1132 BsaWI w'CCGG_w Cuts at: 0 61 1665 3747 Size: 61 1604 2082 Fragments arranged by size: 2082 1604 61 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 748 778 1008 1038 3747 Size: 748 30 230 30 2709 Fragments arranged by size: 2709 748 230 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 34 797 1298 2267 3472 3747 Size: 34 763 501 969 1205 275 Fragments arranged by size: 1205 969 763 501 275 34 BseYI C'CCAG_C Cuts at: 0 1747 3747 Size: 1747 2000 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1610 3747 Size: 1610 2137 BsiHKAI G_wGCw'C Cuts at: 0 116 789 867 1443 3747 Size: 116 673 78 576 2304 Fragments arranged by size: 2304 673 576 116 78 Bsp1286I G_dGCh'C Cuts at: 0 26 116 153 789 867 1257 1443 3390 Size: 26 90 37 636 78 390 186 1947 Cuts at: 3390 3747 Size: 357 Fragments arranged by size: 1947 636 390 357 186 90 78 37 26 BspEI T'CCGG_A Cuts at: 0 1665 3747 Size: 1665 2082 BspHI T'CATG_A Cuts at: 0 1763 2766 3747 Size: 1763 1003 981 Fragments arranged by size: 1763 1003 981 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 153 767 1215 1858 3747 Size: 153 614 448 643 1889 Fragments arranged by size: 1889 643 614 448 153 BsrBI CCG'CTC Cuts at: 0 1875 3747 Size: 1875 1872 BsrDI GCAATG_nn' Cuts at: 0 1289 1373 2198 3296 3747 Size: 1289 84 825 1098 451 Fragments arranged by size: 1289 1098 825 451 84 BsrGI T'GTAC_A Cuts at: 0 1953 3747 Size: 1953 1794 BstBI TT'CG_AA Cuts at: 0 1977 3747 Size: 1977 1770 BstXI CCAn_nnnn'nTGG Cuts at: 0 697 1755 3747 Size: 697 1058 1992 Fragments arranged by size: 1992 1058 697 BstYI r'GATC_y Cuts at: 0 1608 1797 2346 2911 3078 3747 Size: 1608 189 549 565 167 669 Fragments arranged by size: 1608 669 565 549 189 167 Bsu36I CC'TnA_GG Cuts at: 0 318 365 2000 3747 Size: 318 47 1635 1747 Fragments arranged by size: 1747 1635 318 47 BtgI C'CryG_G Cuts at: 0 1590 2256 3747 Size: 1590 666 1491 Fragments arranged by size: 1590 1491 666 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 102 236 2624 3747 Size: 102 134 2388 1123 Fragments arranged by size: 2388 1123 134 102 BtsI GCAGTG_nn' Cuts at: 0 1451 1463 1892 2386 3747 Size: 1451 12 429 494 1361 Fragments arranged by size: 1451 1361 494 429 12 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1347 1382 3747 Size: 1347 35 2365 Fragments arranged by size: 2365 1347 35 DraI TTT'AAA Cuts at: 0 1962 3050 3747 Size: 1962 1088 697 Fragments arranged by size: 1962 1088 697 DraIII CAC_nnn'GTG Cuts at: 0 429 3747 Size: 429 3318 DrdI GACnn_nn'nnGTC Cuts at: 0 136 3747 Size: 136 3611 EaeI y'GGCC_r Cuts at: 0 2 1003 3747 Size: 2 1001 2744 Fragments arranged by size: 2744 1001 2 EarI CTCTTCn'nnn_ Cuts at: 0 276 492 1431 1882 2138 3050 3747 Size: 276 216 939 451 256 912 697 Fragments arranged by size: 939 912 697 451 276 256 216 EcoICRI GAG'CTC Cuts at: 0 787 1441 3747 Size: 787 654 2306 Fragments arranged by size: 2306 787 654 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 1616 1684 1863 2039 2177 2662 3086 Size: 636 980 68 179 176 138 485 424 Cuts at: 3086 3747 Size: 661 Fragments arranged by size: 980 661 636 485 424 179 176 138 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 1865 2171 3747 Size: 1865 306 1576 Fragments arranged by size: 1865 1576 306 EcoO109I rG'GnC_Cy Cuts at: 0 414 1237 1253 1254 2890 3747 Size: 414 823 16 1 1636 857 Fragments arranged by size: 1636 857 823 414 16 1 EcoRI G'AATT_C Cuts at: 0 2113 3747 Size: 2113 1634 EcoRV GAT'ATC Cuts at: 0 198 3747 Size: 198 3549 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1840 1872 1874 1906 2311 2343 3747 Size: 1840 32 2 32 405 32 1404 Fragments arranged by size: 1840 1404 405 32 32 32 2 HincII GTy'rAC Cuts at: 0 141 975 2232 2452 3747 Size: 141 834 1257 220 1295 Fragments arranged by size: 1295 1257 834 220 141 HindIII A'AGCT_T Cuts at: 0 2159 3747 Size: 2159 1588 MfeI C'AATT_G Cuts at: 0 2186 3184 3747 Size: 2186 998 563 Fragments arranged by size: 2186 998 563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1560 3747 Size: 1560 2187 MscI TGG'CCA Cuts at: 0 4 1005 3747 Size: 4 1001 2742 Fragments arranged by size: 2742 1001 4 MslI CAynn'nnrTG Cuts at: 0 962 1456 1792 3747 Size: 962 494 336 1955 Fragments arranged by size: 1955 962 494 336 MspA1I CmG'CkG Cuts at: 0 10 715 779 1329 3747 Size: 10 705 64 550 2418 Fragments arranged by size: 2418 705 550 64 10 NcoI C'CATG_G Cuts at: 0 2256 3747 Size: 2256 1491 NsiI A_TGCA'T Cuts at: 0 1365 2298 3567 3747 Size: 1365 933 1269 180 Fragments arranged by size: 1365 1269 933 180 NspI r_CATG'y Cuts at: 0 162 802 1367 2074 3565 3747 Size: 162 640 565 707 1491 182 Fragments arranged by size: 1491 707 640 565 182 162 PasI CC'CwG_GG Cuts at: 0 1802 3747 Size: 1802 1945 PciI A'CATG_T Cuts at: 0 798 3747 Size: 798 2949 PflMI CCAn_nnn'nTGG Cuts at: 0 256 1012 3747 Size: 256 756 2735 Fragments arranged by size: 2735 756 256 PfoI T'CCnGG_A Cuts at: 0 125 3747 Size: 125 3622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1598 1630 3747 Size: 1598 32 2117 Fragments arranged by size: 2117 1598 32 PpuMI rG'GwC_Cy Cuts at: 0 414 1237 2890 3747 Size: 414 823 1653 857 Fragments arranged by size: 1653 857 823 414 PsiI TTA'TAA Cuts at: 0 952 3125 3747 Size: 952 2173 622 Fragments arranged by size: 2173 952 622 PspOMI G'GGCC_C Cuts at: 0 1253 3747 Size: 1253 2494 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2948 2980 3747 Size: 2948 32 767 Fragments arranged by size: 2948 767 32 PstI C_TGCA'G Cuts at: 0 1458 1899 3747 Size: 1458 441 1848 Fragments arranged by size: 1848 1458 441 PvuII CAG'CTG Cuts at: 0 715 1329 3747 Size: 715 614 2418 Fragments arranged by size: 2418 715 614 SacI G_AGCT'C Cuts at: 0 789 1443 3747 Size: 789 654 2304 Fragments arranged by size: 2304 789 654 SalI G'TCGA_C Cuts at: 0 139 3747 Size: 139 3608 SanDI GG'GwC_CC Cuts at: 0 414 3747 Size: 414 3333 SapI GCTCTTCn'nnn_ Cuts at: 0 492 1431 1882 3747 Size: 492 939 451 1865 Fragments arranged by size: 1865 939 492 451 SexAI A'CCwGG_T Cuts at: 0 1965 3747 Size: 1965 1782 SfcI C'TryA_G Cuts at: 0 236 447 978 1103 1454 1895 2668 3747 Size: 236 211 531 125 351 441 773 1079 Fragments arranged by size: 1079 773 531 441 351 236 211 125 SmaI CCC'GGG Cuts at: 0 146 3747 Size: 146 3601 SmlI C'TyrA_G Cuts at: 0 289 373 491 651 1487 1809 2751 3419 Size: 289 84 118 160 836 322 942 668 Cuts at: 3419 3747 Size: 328 Fragments arranged by size: 942 836 668 328 322 289 160 118 84 SphI G_CATG'C Cuts at: 0 162 3747 Size: 162 3585 StuI AGG'CCT Cuts at: 0 926 3309 3593 3747 Size: 926 2383 284 154 Fragments arranged by size: 2383 926 284 154 StyI C'CwwG_G Cuts at: 0 1257 2172 2256 3747 Size: 1257 915 84 1491 Fragments arranged by size: 1491 1257 915 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2841 3427 3747 Size: 2841 586 320 Fragments arranged by size: 2841 586 320 TatI w'GTAC_w Cuts at: 0 1953 2269 3747 Size: 1953 316 1478 Fragments arranged by size: 1953 1478 316 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1008 1040 3369 3401 3672 3704 3747 Size: 1008 32 2329 32 271 32 43 Fragments arranged by size: 2329 1008 271 43 32 32 32 XbaI T'CTAG_A Cuts at: 0 1231 1443 3747 Size: 1231 212 2304 Fragments arranged by size: 2304 1231 212 XmaI C'CCGG_G Cuts at: 0 144 3747 Size: 144 3603 XmnI GAAnn'nnTTC Cuts at: 0 2162 3747 Size: 2162 1585 Enzymes that do cut: AccI AcuI AflII AflIII AleI AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanII BbsI BciVI BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SacI SalI SanDI SapI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XbaI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaLI AscI AsiSI BaeI BanI BbeI BbvCI BcgI BclI BglI BlpI BmgBI BmtI BplI Bpu10I BsaHI BsiEI BsiWI BsmI BsmBI BsrFI BssHII BssSI BstAPI BstEII BstZ17I ClaI EagI EciI FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PshAI PspXI PvuI RsrII SacII SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XcmI XhoI ZraI