(Circular) (Six-base) MAPSORT of: pFN21AB5724.seq Check: 3175 from: 1 to: 6892 pFN21AB5724 6892 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4702 278 Size: 53 83 186 936 3166 2468 Fragments arranged by size: 3166 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4235 1937 Size: 2298 4594 Acc65I G'GTAC_C Cuts at: 4213 4213 Size: 6892 AclI AA'CG_TT Cuts at: 1563 4179 5019 5392 1563 Size: 2616 840 373 3063 Fragments arranged by size: 3063 2616 840 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2807 3545 4955 6339 6607 1632 Size: 1175 738 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 1175 738 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6695 AflIII A'CryG_T Cuts at: 1203 5791 6768 1203 Size: 4588 977 1327 Fragments arranged by size: 4588 1327 977 AgeI A'CCGG_T Cuts at: 5723 5723 Size: 6892 AhdI GACnn_n'nnGTC Cuts at: 3080 5621 3080 Size: 2541 4351 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6892 AlwNI CAG_nnn'CTG Cuts at: 2333 2651 4635 6207 2333 Size: 318 1984 1572 3018 Fragments arranged by size: 3018 1984 1572 318 ApaLI G'TGCA_C Cuts at: 2399 4949 6105 2399 Size: 2550 1156 3186 Fragments arranged by size: 3186 2550 1156 ApoI r'AATT_y Cuts at: 1493 2168 2264 2336 3192 3495 3755 4201 4427 Size: 675 96 72 856 303 260 446 226 Cuts at: 4427 4579 6688 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 856 675 446 303 260 226 152 96 72 AseI AT'TA_AT Cuts at: 160 1032 3179 3317 3350 5446 160 Size: 872 2147 138 33 2096 1606 Fragments arranged by size: 2147 2096 1606 872 138 33 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 AvaI C'yCGr_G Cuts at: 1870 1943 2814 4217 1870 Size: 73 871 1403 4545 Fragments arranged by size: 4545 1403 871 73 BamHI G'GATC_C Cuts at: 4222 4589 4222 Size: 367 6525 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4213 5668 618 Size: 325 217 627 2426 1455 1842 Fragments arranged by size: 2426 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4211 1391 Size: 2820 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2089 2813 3551 928 Size: 971 190 724 738 4269 Fragments arranged by size: 4269 971 738 724 190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5083 5117 1903 Size: 34 50 34 3062 34 3678 Fragments arranged by size: 3678 3062 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3324 4784 5994 3324 Size: 1460 1210 4222 Fragments arranged by size: 4222 1460 1210 BclI T'GATC_A Cuts at: 1548 3399 1548 Size: 1851 5041 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5503 136 Size: 107 122 71 631 605 3831 1525 Fragments arranged by size: 3831 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6887 6887 Size: 6892 BlpI GC'TnA_GC Cuts at: 4309 4309 Size: 6892 Bme1580I G_kGCm'C Cuts at: 1790 2403 3616 4953 6109 1790 Size: 613 1213 1337 1156 2573 Fragments arranged by size: 2573 1337 1213 1156 613 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6892 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4623 5581 442 Size: 416 53 83 399 24 3206 958 1753 Fragments arranged by size: 3206 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3243 1056 Size: 2187 4705 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2766 2800 3570 5552 1239 Size: 1527 34 770 1982 2579 Fragments arranged by size: 2579 1982 1527 770 34 Bpu10I CC'TnA_GC Cuts at: 3249 3249 Size: 6892 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5017 5882 6180 5017 Size: 865 298 5729 Fragments arranged by size: 5729 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5555 882 Size: 617 4056 2219 Fragments arranged by size: 4056 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4683 493 Size: 711 3479 2702 Fragments arranged by size: 3479 2702 711 BsaBI GATnn'nnATC Cuts at: 3464 6886 3464 Size: 3422 3470 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4699 5081 5716 275 Size: 53 83 186 936 3166 382 635 1451 Fragments arranged by size: 3166 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5325 5723 5997 6144 6555 5325 Size: 398 274 147 411 5662 Fragments arranged by size: 5662 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2592 2622 1178 Size: 30 165 30 1189 30 5448 Fragments arranged by size: 5448 1189 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 1186 Size: 1057 5835 BseYI C'CCAG_C Cuts at: 6095 6591 6095 Size: 496 6396 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2502 1163 Size: 573 766 5553 Fragments arranged by size: 5553 766 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4342 5103 6131 1540 Size: 275 186 2341 761 1028 2301 Fragments arranged by size: 2341 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2015 2403 4953 5038 6109 2015 Size: 388 2550 85 1071 2798 Fragments arranged by size: 2798 2550 1071 388 85 BsmI GAATG_Cn' Cuts at: 4403 4496 4403 Size: 93 6799 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2403 3616 4211 4953 5038 6109 Size: 399 225 388 1213 595 742 85 1071 Cuts at: 6109 1391 Size: 2174 Fragments arranged by size: 2174 1213 1071 742 595 399 388 225 85 BspEI T'CCGG_A Cuts at: 6555 6555 Size: 6892 BspHI T'CATG_A Cuts at: 3010 4781 6511 3010 Size: 1771 1730 3391 Fragments arranged by size: 3391 1771 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4247 844 Size: 3403 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4779 1394 Size: 429 2956 3507 Fragments arranged by size: 3507 2956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5387 5561 65 Size: 1183 4139 174 1396 Fragments arranged by size: 4139 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5536 5723 1700 Size: 110 3726 187 2869 Fragments arranged by size: 3726 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2460 96 Size: 2364 4528 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6892 BssSI C'ACGA_G Cuts at: 4952 5964 4952 Size: 1012 5880 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6892 BstBI TT'CG_AA Cuts at: 5696 5696 Size: 6892 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6892 BstXI CCAn_nnnn'nTGG Cuts at: 2778 2778 Size: 6892 BstYI r'GATC_y Cuts at: 1972 4222 4589 4974 4991 6443 6529 6541 6887 Size: 2250 367 385 17 1452 86 12 346 Cuts at: 6887 1972 Size: 1977 Fragments arranged by size: 2250 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4158 513 Size: 552 3093 3247 Fragments arranged by size: 3247 3093 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6153 Fragments arranged by size: 6153 683 56 BtsI GCAGTG_nn' Cuts at: 2574 2657 4408 5202 5222 2574 Size: 83 1751 794 20 4244 Fragments arranged by size: 4244 1751 794 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6892 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6857 DraI TTT'AAA Cuts at: 4196 4196 Size: 6892 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5899 809 Size: 507 4583 1802 Fragments arranged by size: 4583 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4339 5228 6857 Size: 54 1425 50 275 18 2509 889 1629 Cuts at: 6857 8 Size: 43 Fragments arranged by size: 2509 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4339 1537 Size: 275 2527 4090 Fragments arranged by size: 4090 2527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 4083 4822 1353 Size: 867 1863 739 3423 Fragments arranged by size: 3423 1863 867 739 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5479 5853 5999 387 Size: 1064 138 3890 374 146 1280 Fragments arranged by size: 3890 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2766 2800 2807 3545 3570 4955 5552 Size: 393 1134 34 7 738 25 1385 597 Cuts at: 5552 6339 6607 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1385 1134 787 738 597 393 268 34 25 7 EcoO109I rG'GnC_Cy Cuts at: 2681 2681 Size: 6892 EcoRI G'AATT_C Cuts at: 2336 4201 4579 2336 Size: 1865 378 4649 Fragments arranged by size: 4649 1865 378 EcoRV GAT'ATC Cuts at: 2410 3568 2410 Size: 1158 5734 FspI TGC'GCA Cuts at: 5398 5398 Size: 6892 HaeII r_GCGC'y Cuts at: 1678 6039 1678 Size: 4361 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 3066 3098 3679 Size: 32 135 32 866 32 596 32 581 Cuts at: 3679 3711 3862 3894 5533 5565 5607 5639 1373 Size: 32 151 32 1639 32 42 32 2626 Fragments arranged by size: 2626 1639 866 596 581 151 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4183 4236 4482 669 Size: 988 281 2245 53 246 3079 Fragments arranged by size: 3079 2245 988 281 246 53 HindIII A'AGCT_T Cuts at: 748 2134 748 Size: 1386 5506 HpaI GTT'AAC Cuts at: 4482 4482 Size: 6892 KpnI G_GTAC'C Cuts at: 4217 4217 Size: 6892 MfeI C'AATT_G Cuts at: 2772 4491 2772 Size: 1719 5173 MluI A'CGCG_T Cuts at: 6768 6768 Size: 6892 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6006 6190 1305 Size: 4701 184 2007 Fragments arranged by size: 4701 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5070 Fragments arranged by size: 5070 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4850 5209 5368 518 Size: 648 3684 359 159 2042 Fragments arranged by size: 3684 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4308 4985 6133 6378 1606 Size: 2702 677 1148 245 2120 Fragments arranged by size: 2702 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 4158 513 Size: 552 3093 3247 Fragments arranged by size: 3247 3093 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6892 NheI G'CTAG_C Cuts at: 1052 3239 1052 Size: 2187 4705 NotI GC'GGCC_GC Cuts at: 4339 4339 Size: 6892 NspI r_CATG'y Cuts at: 1132 1229 2794 4250 5795 1132 Size: 97 1565 1456 1545 2229 Fragments arranged by size: 2229 1565 1545 1456 97 PacI TTA_AT'TAA Cuts at: 3354 3354 Size: 6892 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6892 PciI A'CATG_T Cuts at: 5791 5791 Size: 6892 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6892 PmeI GTTT'AAAC Cuts at: 4196 4196 Size: 6892 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4961 4993 6474 6506 4961 Size: 32 1481 32 5347 Fragments arranged by size: 5347 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6892 PsiI TTA'TAA Cuts at: 3735 4462 3735 Size: 727 6165 PstI C_TGCA'G Cuts at: 830 3284 4244 830 Size: 2454 960 3478 Fragments arranged by size: 3478 2454 960 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6892 SalI G'TCGA_C Cuts at: 1936 4234 1936 Size: 2298 4594 SbfI CC_TGCA'GG Cuts at: 4244 4244 Size: 6892 ScaI AGT'ACT Cuts at: 2253 5140 6852 2253 Size: 2887 1712 2293 Fragments arranged by size: 2887 2293 1712 SfcI C'TryA_G Cuts at: 826 1045 3280 4240 5375 6056 6247 826 Size: 219 2235 960 1135 681 191 1471 Fragments arranged by size: 2235 1471 1135 960 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6892 SmaI CCC'GGG Cuts at: 1872 4219 1872 Size: 2347 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4996 5897 6159 820 Size: 197 926 3053 901 262 1553 Fragments arranged by size: 3053 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4683 493 Size: 4190 2702 SpeI A'CTAG_T Cuts at: 152 2909 152 Size: 2757 4135 SphI G_CATG'C Cuts at: 1132 4250 1132 Size: 3118 3774 SspI AAT'ATT Cuts at: 5 52 2203 2540 4816 5 Size: 47 2151 337 2276 2081 Fragments arranged by size: 2276 2151 2081 337 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6892 StyI C'CwwG_G Cuts at: 513 1065 2053 4158 4331 513 Size: 552 988 2105 173 3074 Fragments arranged by size: 3074 2105 988 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 4919 4936 5089 5274 5719 325 Size: 1255 774 2565 17 153 185 445 1498 Fragments arranged by size: 2565 1498 1255 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2251 2460 2749 Size: 274 80 33 51 1443 274 209 289 Cuts at: 2749 3208 5138 6850 96 Size: 459 1930 1712 138 Fragments arranged by size: 1930 1712 1443 459 289 274 274 209 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 3096 3128 6852 6884 2442 Size: 32 622 32 3724 32 2450 Fragments arranged by size: 3724 2450 622 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6892 XbaI T'CTAG_A Cuts at: 4228 4228 Size: 6892 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2648 2648 Size: 6892 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6892 XmaI C'CCGG_G Cuts at: 1870 4217 1870 Size: 2347 4545 XmnI GAAnn'nnTTC Cuts at: 2728 3375 5021 2728 Size: 647 1646 4599 Fragments arranged by size: 4599 1646 647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4700 276 Size: 53 83 186 936 3166 2468 Fragments arranged by size: 3166 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI