(Circular) (Six-base) MAPSORT of: pFN21AE1874.seq Check: 1937 from: 1 to: 5893 pFN21AE1874 5893 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2526 3236 1937 Size: 589 710 4594 Fragments arranged by size: 4594 710 589 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3956 5340 5608 1632 Size: 2324 1384 268 1917 Fragments arranged by size: 2324 1917 1384 268 AfeI AGC'GCT Cuts at: 2222 2222 Size: 5893 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 2352 2553 4792 5769 1203 Size: 1149 201 2239 977 1327 Fragments arranged by size: 2239 1327 1149 977 201 AgeI A'CCGG_T Cuts at: 2866 4724 2866 Size: 1858 4035 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 2717 1166 Size: 1551 4342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2816 1184 Size: 1632 4261 AlwNI CAG_nnn'CTG Cuts at: 3636 5208 3636 Size: 1572 4321 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 3202 3428 3580 5689 1493 Size: 1709 226 152 2109 1697 Fragments arranged by size: 2109 1709 1697 226 152 AscI GG'CGCG_CC Cuts at: 2492 2492 Size: 5893 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 2832 3218 1870 Size: 73 889 386 4545 Fragments arranged by size: 4545 889 386 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 2177 2707 2814 2842 Size: 325 217 627 313 77 530 107 28 Cuts at: 2842 2908 3214 4669 618 Size: 66 306 1455 1842 Fragments arranged by size: 1842 1455 627 530 325 313 306 217 107 77 66 28 BanII G_rGCy'C Cuts at: 1391 2216 3212 1391 Size: 825 996 4072 Fragments arranged by size: 4072 996 825 BbeI G_GCGC'C Cuts at: 2104 2181 2846 2912 2104 Size: 77 665 66 5085 Fragments arranged by size: 5085 665 77 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2104 2138 2420 2454 3124 3158 4084 Size: 34 167 34 282 34 670 34 926 Cuts at: 4084 4118 1903 Size: 34 3678 Fragments arranged by size: 3678 926 670 282 167 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BfrBI ATG'CAT Cuts at: 3145 3145 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2951 4504 136 Size: 107 122 71 631 605 1279 1553 1525 Fragments arranged by size: 1553 1525 1279 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 3172 3310 3172 Size: 138 5755 Bme1580I G_kGCm'C Cuts at: 1790 2154 2241 2712 3954 5110 1790 Size: 364 87 471 1242 1156 2573 Fragments arranged by size: 2573 1242 1156 471 364 87 BmgBI CAC'GTC Cuts at: 1318 2548 1318 Size: 1230 4663 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2530 2683 3197 Size: 416 53 83 399 24 1113 153 514 Cuts at: 3197 3624 4582 442 Size: 427 958 1753 Fragments arranged by size: 1753 1113 958 514 427 416 399 153 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3065 4553 1239 Size: 1826 1488 2579 Fragments arranged by size: 2579 1826 1488 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 5893 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3103 4018 4883 5181 3103 Size: 915 865 298 3815 Fragments arranged by size: 3815 915 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2768 4556 882 Size: 617 1269 1788 2219 Fragments arranged by size: 2219 1788 1269 617 BsaAI yAC'GTr Cuts at: 493 1204 2554 3684 493 Size: 711 1350 1130 2702 Fragments arranged by size: 2702 1350 1130 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2101 2178 2843 2909 Size: 53 83 186 936 568 77 665 66 Cuts at: 2909 3700 4082 4717 275 Size: 791 382 635 1451 Fragments arranged by size: 1451 936 791 665 635 568 382 186 83 77 66 53 BsaWI w'CCGG_w Cuts at: 2866 4326 4724 4998 5145 5556 2866 Size: 1460 398 274 147 411 3203 Fragments arranged by size: 3203 1460 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2661 2691 2765 2795 1178 Size: 30 165 30 1258 30 74 30 4276 Fragments arranged by size: 4276 1258 165 74 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 3152 3194 1186 Size: 1102 864 42 3885 Fragments arranged by size: 3885 1102 864 42 BseYI C'CCAG_C Cuts at: 2388 2891 5096 5592 2388 Size: 503 2205 496 2689 Fragments arranged by size: 2689 2205 503 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2645 3077 1163 Size: 573 909 432 3979 Fragments arranged by size: 3979 909 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2293 2470 2688 3343 4104 5132 Size: 275 186 292 177 218 655 761 1028 Cuts at: 5132 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 655 292 275 218 186 177 BsiHKAI G_wGCw'C Cuts at: 3954 4039 5110 3954 Size: 85 1071 4737 Fragments arranged by size: 4737 1071 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2216 2241 2712 3212 3954 4039 Size: 399 364 62 25 471 500 742 85 Cuts at: 4039 5110 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 500 471 399 364 85 62 25 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2167 2320 2634 2641 2831 2986 3780 Size: 429 344 153 314 7 190 155 794 Cuts at: 3780 1394 Size: 3507 Fragments arranged by size: 3507 794 429 344 314 190 155 153 7 BsrDI GCAATG_nn' Cuts at: 65 1248 2337 4388 4562 65 Size: 1183 1089 2051 174 1396 Fragments arranged by size: 2051 1396 1183 1089 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 2097 2510 2716 2839 2866 2880 Size: 110 279 8 413 206 123 27 14 Cuts at: 2880 4537 4724 1700 Size: 1657 187 2869 Fragments arranged by size: 2869 1657 413 279 206 187 123 110 27 14 8 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 2111 2492 2876 2936 1925 Size: 186 381 384 60 4882 Fragments arranged by size: 4882 384 381 186 60 BssSI C'ACGA_G Cuts at: 2123 2234 3953 4965 2123 Size: 111 1719 1012 3051 Fragments arranged by size: 3051 1719 1012 111 BstAPI GCAn_nnn'nTGC Cuts at: 794 2125 2149 794 Size: 1331 24 4538 Fragments arranged by size: 4538 1331 24 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 3137 3137 Size: 5893 BstYI r'GATC_y Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888 Size: 1251 367 385 17 1452 86 12 346 Cuts at: 5888 1972 Size: 1977 Fragments arranged by size: 1977 1452 1251 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2665 2967 513 Size: 552 939 661 302 3439 Fragments arranged by size: 3439 939 661 552 302 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 2508 3409 4203 4223 2508 Size: 901 794 20 4178 Fragments arranged by size: 4178 901 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DraIII CAC_nnn'GTG Cuts at: 2821 2821 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2334 2401 Size: 54 1425 50 275 18 265 239 67 Cuts at: 2401 2685 2930 3340 4229 5858 8 Size: 284 245 410 889 1629 43 Fragments arranged by size: 1629 1425 889 410 284 275 265 245 239 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2685 3340 1537 Size: 275 873 655 4090 Fragments arranged by size: 4090 873 655 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 3823 1353 Size: 1379 1091 3423 Fragments arranged by size: 3423 1379 1091 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3065 3956 4553 5340 5608 1239 Size: 393 1433 891 597 787 268 1524 Fragments arranged by size: 1524 1433 891 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2230 2230 Size: 5893 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 2057 2104 2181 2224 2752 2846 2912 3110 Size: 379 47 77 43 528 94 66 198 Cuts at: 3110 5040 1678 Size: 1930 2531 Fragments arranged by size: 2531 1930 528 379 198 94 77 66 47 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4534 4566 4608 4640 1373 Size: 32 135 32 2962 32 42 32 2626 Fragments arranged by size: 2962 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3237 3483 669 Size: 988 281 1299 246 3079 Fragments arranged by size: 3079 1299 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KasI G'GCGC_C Cuts at: 2100 2177 2842 2908 2100 Size: 77 665 66 5085 Fragments arranged by size: 5085 665 77 66 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5007 5191 1305 Size: 3702 184 2007 Fragments arranged by size: 3702 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2336 10 Size: 54 1425 343 504 3567 Fragments arranged by size: 3567 1425 504 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 2717 3851 4210 4369 518 Size: 648 1176 375 1134 359 159 2042 Fragments arranged by size: 2042 1176 1134 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2192 2667 3309 3986 5134 5379 1606 Size: 586 475 642 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 586 475 245 NaeI GCC'GGC Cuts at: 2099 2512 2841 2882 2099 Size: 413 329 41 5110 Fragments arranged by size: 5110 413 329 41 NarI GG'CG_CC Cuts at: 2101 2178 2843 2909 2101 Size: 77 665 66 5085 Fragments arranged by size: 5085 665 77 66 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4402 Fragments arranged by size: 4402 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NgoMIV G'CCGG_C Cuts at: 2097 2510 2839 2880 2097 Size: 413 329 41 5110 Fragments arranged by size: 5110 413 329 41 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NsiI A_TGCA'T Cuts at: 3147 3147 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 2356 3145 3251 4796 1132 Size: 97 1127 789 106 1545 2229 Fragments arranged by size: 2229 1545 1127 789 106 97 PasI CC'CwG_GG Cuts at: 1348 2173 1348 Size: 825 5068 PciI A'CATG_T Cuts at: 2352 4792 2352 Size: 2440 3453 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5893 PfoI T'CCnGG_A Cuts at: 2226 2605 2730 2226 Size: 379 125 5389 Fragments arranged by size: 5389 379 125 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PmlI CAC'GTG Cuts at: 2554 2554 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2230 Size: 5893 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PspXI vC'TCGA_Gb Cuts at: 2832 2832 Size: 5893 PstI C_TGCA'G Cuts at: 830 3245 830 Size: 2415 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 RsrII CG'GwC_CG Cuts at: 2584 2584 Size: 5893 SacII CC_GC'GG Cuts at: 2668 2668 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SapI GCTCTTCn'nnn_ Cuts at: 2732 2732 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SexAI A'CCwGG_T Cuts at: 2817 2817 Size: 5893 SfcI C'TryA_G Cuts at: 826 1045 3241 4376 5057 5248 826 Size: 219 2196 1135 681 191 1471 Fragments arranged by size: 2196 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2102 2179 2844 2910 2102 Size: 77 665 66 5085 Fragments arranged by size: 5085 665 77 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 2716 2866 1700 Size: 1016 150 4727 Fragments arranged by size: 4727 1016 150 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2832 3082 3997 4898 5160 820 Size: 197 926 889 250 915 901 262 1553 Fragments arranged by size: 1553 926 915 901 889 262 250 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3145 3251 1132 Size: 2013 106 3774 Fragments arranged by size: 3774 2013 106 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2004 3332 513 Size: 552 939 1328 3074 Fragments arranged by size: 3074 1328 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 2548 2792 3920 3937 4090 4275 Size: 1255 738 230 244 1128 17 153 185 Cuts at: 4275 4720 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1128 738 445 244 230 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4139 5851 96 Size: 274 80 33 51 1443 2162 1712 138 Fragments arranged by size: 2162 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2568 2600 2765 2797 5853 5885 2568 Size: 32 165 32 3056 32 2576 Fragments arranged by size: 3056 2576 165 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2522 1316 Size: 1206 4687 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 2832 1943 Size: 889 5004 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 4022 4022 Size: 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PspOMI PsrI PvuII SacI SanDI SfiI SrfI SwaI XcmI