(Linear) (Six-base) MAPSORT of: KIEE1874.seq Check: 8232 from: 1 to: 1188 KIEE1874.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AccI GT'mk_AC Cuts at: 0 521 1188 Size: 521 667 AfeI AGC'GCT Cuts at: 0 217 1188 Size: 217 971 AflIII A'CryG_T Cuts at: 0 347 548 1188 Size: 347 201 640 Fragments arranged by size: 640 347 201 AgeI A'CCGG_T Cuts at: 0 861 1188 Size: 861 327 AleI CACnn'nnGTG Cuts at: 0 712 1188 Size: 712 476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 811 1188 Size: 811 377 AscI GG'CGCG_CC Cuts at: 0 487 1188 Size: 487 701 AvaI C'yCGr_G Cuts at: 0 827 1188 Size: 827 361 BanI G'GyrC_C Cuts at: 0 95 172 702 809 837 903 1188 Size: 95 77 530 107 28 66 285 Fragments arranged by size: 530 285 107 95 77 66 28 BanII G_rGCy'C Cuts at: 0 211 1188 Size: 211 977 BbeI G_GCGC'C Cuts at: 0 99 176 841 907 1188 Size: 99 77 665 66 281 Fragments arranged by size: 665 281 99 77 66 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 99 133 415 449 1119 1153 1188 Size: 99 34 282 34 670 34 35 Fragments arranged by size: 670 282 99 35 34 34 34 BfrBI ATG'CAT Cuts at: 0 1140 1188 Size: 1140 48 BglI GCCn_nnn'nGGC Cuts at: 0 946 1188 Size: 946 242 BlpI GC'TnA_GC Cuts at: 0 1167 1188 Size: 1167 21 Bme1580I G_kGCm'C Cuts at: 0 149 236 707 1188 Size: 149 87 471 481 Fragments arranged by size: 481 471 149 87 BmgBI CAC'GTC Cuts at: 0 543 1188 Size: 543 645 BmrI ACTGGGnnnn_n' Cuts at: 0 525 678 1188 Size: 525 153 510 Fragments arranged by size: 525 510 153 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1060 1188 Size: 1060 128 Bpu10I CC'TnA_GC Cuts at: 0 407 1188 Size: 407 781 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1098 1188 Size: 1098 90 BsaI GGTCTCn'nnnn_ Cuts at: 0 763 1188 Size: 763 425 BsaAI yAC'GTr Cuts at: 0 549 1188 Size: 549 639 BsaHI Gr'CG_yC Cuts at: 0 96 173 838 904 1188 Size: 96 77 665 66 284 Fragments arranged by size: 665 284 96 77 66 BsaWI w'CCGG_w Cuts at: 0 861 1188 Size: 861 327 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 656 686 760 790 1188 Size: 656 30 74 30 398 Fragments arranged by size: 656 398 74 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 283 1147 1188 Size: 283 864 41 Fragments arranged by size: 864 283 41 BseYI C'CCAG_C Cuts at: 0 383 886 1188 Size: 383 503 302 Fragments arranged by size: 503 383 302 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1072 1188 Size: 640 432 116 Fragments arranged by size: 640 432 116 BsiEI CG_ry'CG Cuts at: 0 288 465 683 1188 Size: 288 177 218 505 Fragments arranged by size: 505 288 218 177 Bsp1286I G_dGCh'C Cuts at: 0 149 211 236 707 1188 Size: 149 62 25 471 481 Fragments arranged by size: 481 471 149 62 25 BsrBI CCG'CTC Cuts at: 0 162 315 629 636 826 981 1188 Size: 162 153 314 7 190 155 207 Fragments arranged by size: 314 207 190 162 155 153 7 BsrDI GCAATG_nn' Cuts at: 0 332 1188 Size: 332 856 BsrFI r'CCGG_y Cuts at: 0 84 92 505 711 834 861 875 1188 Size: 84 8 413 206 123 27 14 313 Fragments arranged by size: 413 313 206 123 84 27 14 8 BssHII G'CGCG_C Cuts at: 0 106 487 871 931 1188 Size: 106 381 384 60 257 Fragments arranged by size: 384 381 257 106 60 BssSI C'ACGA_G Cuts at: 0 118 229 1188 Size: 118 111 959 Fragments arranged by size: 959 118 111 BstAPI GCAn_nnn'nTGC Cuts at: 0 120 144 1188 Size: 120 24 1044 Fragments arranged by size: 1044 120 24 BstXI CCAn_nnnn'nTGG Cuts at: 0 1132 1188 Size: 1132 56 BtgI C'CryG_G Cuts at: 0 660 962 1188 Size: 660 302 226 Fragments arranged by size: 660 302 226 BtsI GCAGTG_nn' Cuts at: 0 503 1188 Size: 503 685 DraIII CAC_nnn'GTG Cuts at: 0 816 1188 Size: 816 372 EaeI y'GGCC_r Cuts at: 0 90 329 396 680 925 1188 Size: 90 239 67 284 245 263 Fragments arranged by size: 284 263 245 239 90 67 EagI C'GGCC_G Cuts at: 0 680 1188 Size: 680 508 EarI CTCTTCn'nnn_ Cuts at: 0 727 1188 Size: 727 461 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1060 1188 Size: 1060 128 EcoO109I rG'GnC_Cy Cuts at: 0 225 1188 Size: 225 963 HaeII r_GCGC'y Cuts at: 0 52 99 176 219 747 841 907 1105 Size: 52 47 77 43 528 94 66 198 Cuts at: 1105 1188 Size: 83 Fragments arranged by size: 528 198 94 83 77 66 52 47 43 KasI G'GCGC_C Cuts at: 0 95 172 837 903 1188 Size: 95 77 665 66 285 Fragments arranged by size: 665 285 95 77 66 MscI TGG'CCA Cuts at: 0 331 1188 Size: 331 857 MslI CAynn'nnrTG Cuts at: 0 337 712 1188 Size: 337 375 476 Fragments arranged by size: 476 375 337 MspA1I CmG'CkG Cuts at: 0 187 662 1188 Size: 187 475 526 Fragments arranged by size: 526 475 187 NaeI GCC'GGC Cuts at: 0 94 507 836 877 1188 Size: 94 413 329 41 311 Fragments arranged by size: 413 329 311 94 41 NarI GG'CG_CC Cuts at: 0 96 173 838 904 1188 Size: 96 77 665 66 284 Fragments arranged by size: 665 284 96 77 66 NgoMIV G'CCGG_C Cuts at: 0 92 505 834 875 1188 Size: 92 413 329 41 313 Fragments arranged by size: 413 329 313 92 41 NsiI A_TGCA'T Cuts at: 0 1142 1188 Size: 1142 46 NspI r_CATG'y Cuts at: 0 351 1140 1188 Size: 351 789 48 Fragments arranged by size: 789 351 48 PasI CC'CwG_GG Cuts at: 0 168 1188 Size: 168 1020 PciI A'CATG_T Cuts at: 0 347 1188 Size: 347 841 PfoI T'CCnGG_A Cuts at: 0 221 600 725 1188 Size: 221 379 125 463 Fragments arranged by size: 463 379 221 125 PmlI CAC'GTG Cuts at: 0 549 1188 Size: 549 639 PpuMI rG'GwC_Cy Cuts at: 0 225 1188 Size: 225 963 PspXI vC'TCGA_Gb Cuts at: 0 827 1188 Size: 827 361 RsrII CG'GwC_CG Cuts at: 0 579 1188 Size: 579 609 SacII CC_GC'GG Cuts at: 0 663 1188 Size: 663 525 SapI GCTCTTCn'nnn_ Cuts at: 0 727 1188 Size: 727 461 SexAI A'CCwGG_T Cuts at: 0 812 1188 Size: 812 376 SfoI GGC'GCC Cuts at: 0 97 174 839 905 1188 Size: 97 77 665 66 283 Fragments arranged by size: 665 283 97 77 66 SgrAI Cr'CCGG_yG Cuts at: 0 711 861 1188 Size: 711 150 327 Fragments arranged by size: 711 327 150 SmlI C'TyrA_G Cuts at: 0 827 1077 1188 Size: 827 250 111 Fragments arranged by size: 827 250 111 SphI G_CATG'C Cuts at: 0 1140 1188 Size: 1140 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 313 543 787 1188 Size: 313 230 244 401 Fragments arranged by size: 401 313 244 230 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 563 595 760 792 1188 Size: 563 32 165 32 396 Fragments arranged by size: 563 396 165 32 32 Tth111I GACn'n_nGTC Cuts at: 0 517 1188 Size: 517 671 XhoI C'TCGA_G Cuts at: 0 827 1188 Size: 827 361 Enzymes that do cut: AccI AfeI AflIII AgeI AleI AlfI AscI AvaI BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BtgI BtsI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI PfoI PmlI PpuMI PspXI RsrII SacII SapI SexAI SfoI SgrAI SmlI SphI TaqII TstI Tth111I XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BglII BmtI BplI BsaBI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI PacI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI TatI XbaI XcmI XmaI XmnI ZraI