(Circular) (Six-base) MAPSORT of: pFN21AE2425.seq Check: 9597 from: 1 to: 5692 pFN21AE2425 5692 bp With 182 enzymes: SgfI * November 22, 2012 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2609 2609 Size: 5692 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2308 2462 3755 5139 5407 1632 Size: 676 154 1293 1384 268 1917 Fragments arranged by size: 1917 1384 1293 676 268 154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 2254 1166 Size: 1088 4604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2321 1184 Size: 1137 4555 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2522 2554 2522 Size: 32 5660 AlwNI CAG_nnn'CTG Cuts at: 3435 5007 3435 Size: 1572 4120 ApaI G_GGCC'C Cuts at: 2096 2393 2473 2588 2096 Size: 297 80 115 5200 Fragments arranged by size: 5200 297 115 80 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2096 3017 1870 Size: 73 123 30 921 4545 Fragments arranged by size: 4545 921 123 73 30 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2726 3013 4468 618 Size: 325 217 627 939 287 1455 1842 Fragments arranged by size: 1842 1455 939 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 2096 2393 2473 2588 3011 1391 Size: 705 297 80 115 423 4072 Fragments arranged by size: 4072 705 423 297 115 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2713 928 Size: 971 814 3907 Fragments arranged by size: 3907 971 814 BbvCI CC'TCA_GC Cuts at: 2132 2132 Size: 5692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2059 4303 Size: 107 122 71 631 605 337 50 2244 Cuts at: 4303 136 Size: 1525 Fragments arranged by size: 2244 1525 631 605 337 122 107 71 50 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2096 2393 2473 2588 3753 4909 1790 Size: 306 297 80 115 1165 1156 2573 Fragments arranged by size: 2573 1165 1156 306 297 115 80 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3423 4381 442 Size: 416 53 83 399 24 2006 958 1753 Fragments arranged by size: 2006 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2290 2351 2885 4352 1239 Size: 1051 61 534 1467 2579 Fragments arranged by size: 2579 1467 1051 534 61 Bpu10I CC'TnA_GC Cuts at: 2132 2132 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2152 3483 493 Size: 711 948 1331 2702 Fragments arranged by size: 2702 1331 948 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2522 2552 1178 Size: 30 165 30 1119 30 4318 Fragments arranged by size: 4318 1119 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 4335 BseYI C'CCAG_C Cuts at: 2014 2080 2109 2344 2812 4895 5391 2014 Size: 66 29 235 468 2083 496 2315 Fragments arranged by size: 2315 2083 496 468 235 66 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2054 2063 3142 3903 4931 1540 Size: 275 186 53 9 1079 761 1028 2301 Fragments arranged by size: 2301 1079 1028 761 275 186 53 9 BsiHKAI G_wGCw'C Cuts at: 2838 2990 3753 3838 4909 2838 Size: 152 763 85 1071 3621 Fragments arranged by size: 3621 1071 763 152 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2393 2473 2588 2838 2990 3011 Size: 399 306 297 80 115 250 152 21 Cuts at: 3011 3753 3838 4909 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 297 250 152 115 85 80 21 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2609 3047 844 Size: 1765 438 3489 Fragments arranged by size: 3489 1765 438 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2463 2608 2959 3579 1394 Size: 429 236 404 145 351 620 3507 Fragments arranged by size: 3507 620 429 404 351 236 145 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4361 65 Size: 1183 2939 174 1396 Fragments arranged by size: 2939 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2773 4336 4523 1700 Size: 110 963 1563 187 2869 Fragments arranged by size: 2869 1563 963 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 2867 794 Size: 2073 3619 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5692 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2199 513 Size: 552 939 195 4006 Fragments arranged by size: 4006 939 552 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 2733 3208 4002 4022 2733 Size: 475 794 20 4403 Fragments arranged by size: 4403 794 475 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2051 2060 3139 Size: 54 1425 50 275 18 221 9 1079 Cuts at: 3139 4028 5657 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1079 889 275 221 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2051 2060 3139 1537 Size: 275 239 9 1079 4090 Fragments arranged by size: 4090 1079 275 239 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2168 2750 2891 3622 1353 Size: 815 582 141 731 3423 Fragments arranged by size: 3423 815 731 582 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2648 4279 4653 4799 387 Size: 1064 138 1059 1631 374 146 1280 Fragments arranged by size: 1631 1280 1064 1059 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2308 2351 2462 2885 3755 4352 Size: 393 658 18 43 111 423 870 597 Cuts at: 4352 5139 5407 1239 Size: 787 268 1524 Fragments arranged by size: 1524 870 787 658 597 423 393 268 111 43 18 EcoO109I rG'GnC_Cy Cuts at: 2093 2585 2093 Size: 492 5200 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 5692 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 2015 2081 2354 4839 1678 Size: 337 66 273 2485 2531 Fragments arranged by size: 2531 2485 337 273 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4333 4365 4407 4439 1373 Size: 32 135 32 2761 32 42 32 2626 Fragments arranged by size: 2761 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2254 2315 2486 3650 4009 4168 518 Size: 648 1088 61 171 1164 359 159 2042 Fragments arranged by size: 2042 1164 1088 648 359 171 159 61 MspA1I CmG'CkG Cuts at: 1606 2201 2348 2475 2521 2650 2717 2816 3108 Size: 595 147 127 46 129 67 99 292 Cuts at: 3108 3785 4933 5178 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 292 245 147 129 127 99 67 46 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4201 Fragments arranged by size: 4201 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 2060 3139 2060 Size: 1079 4613 NspI r_CATG'y Cuts at: 1132 1229 2491 3050 4595 1132 Size: 97 1262 559 1545 2229 Fragments arranged by size: 2229 1545 1262 559 97 PasI CC'CwG_GG Cuts at: 1348 2187 2872 1348 Size: 839 685 4168 Fragments arranged by size: 4168 839 685 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2522 2554 3761 3793 5274 5306 2522 Size: 32 1207 32 1481 32 2908 Fragments arranged by size: 2908 1481 1207 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2038 1613 Size: 425 5267 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2092 2389 2469 2584 2092 Size: 297 80 115 5200 Fragments arranged by size: 5200 297 115 80 PstI C_TGCA'G Cuts at: 830 3044 830 Size: 2214 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2650 2717 2816 2650 Size: 67 99 5526 Fragments arranged by size: 5526 99 67 SacII CC_GC'GG Cuts at: 2202 2202 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SapI GCTCTTCn'nnn_ Cuts at: 2168 2168 Size: 5692 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 3040 4175 4856 5047 826 Size: 219 1995 1135 681 191 1471 Fragments arranged by size: 1995 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2059 2059 Size: 5692 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 2098 3019 1872 Size: 226 921 4545 Fragments arranged by size: 4545 921 226 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2004 2829 3131 513 Size: 552 939 825 302 3074 Fragments arranged by size: 3074 939 825 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2101 2853 2864 3719 3736 3889 4074 Size: 1255 521 752 11 855 17 153 185 Cuts at: 4074 4519 325 Size: 445 1498 Fragments arranged by size: 1498 1255 855 752 521 445 185 153 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5652 5684 5652 Size: 32 5660 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2230 2011 Size: 219 5473 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 2096 3017 1870 Size: 226 921 4545 Fragments arranged by size: 4545 921 226 XmnI GAAnn'nnTTC Cuts at: 2681 3821 2681 Size: 1140 4552 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI