(Circular) (Six-base) MAPSORT of: pFN21AE0054.seq Check: 7905 from: 1 to: 7744 pFN21AE0054 7744 bp With 182 enzymes: SgfI * September 11, 2017 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5554 278 Size: 53 83 186 936 4018 2468 Fragments arranged by size: 4018 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4701 5087 1937 Size: 2764 386 4594 Fragments arranged by size: 4594 2764 386 Acc65I G'GTAC_C Cuts at: 5065 5065 Size: 7744 AclI AA'CG_TT Cuts at: 1563 5871 6244 1563 Size: 4308 373 3063 Fragments arranged by size: 4308 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 2361 4278 4289 4609 5807 7191 7459 Size: 534 195 1917 11 320 1198 1384 268 Cuts at: 7459 1632 Size: 1917 Fragments arranged by size: 1917 1917 1384 1198 534 320 268 195 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7547 AflIII A'CryG_T Cuts at: 1203 6643 7620 1203 Size: 5440 977 1327 Fragments arranged by size: 5440 1327 977 AgeI A'CCGG_T Cuts at: 6575 6575 Size: 7744 AhdI GACnn_n'nnGTC Cuts at: 6473 6473 Size: 7744 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7744 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3204 3420 1184 Size: 2020 216 5508 Fragments arranged by size: 5508 2020 216 AlwNI CAG_nnn'CTG Cuts at: 3428 5487 7059 3428 Size: 2059 1572 4113 Fragments arranged by size: 4113 2059 1572 ApaI G_GGCC'C Cuts at: 4771 4771 Size: 7744 ApaLI G'TGCA_C Cuts at: 2777 4478 4754 5801 6957 2777 Size: 1701 276 1047 1156 3564 Fragments arranged by size: 3564 1701 1156 1047 276 ApoI r'AATT_y Cuts at: 1493 2726 3066 3223 3236 3501 3818 5053 5279 Size: 1233 340 157 13 265 317 1235 226 Cuts at: 5279 5431 7540 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1235 1233 340 317 265 226 157 152 13 AseI AT'TA_AT Cuts at: 160 1032 6298 160 Size: 872 5266 1606 Fragments arranged by size: 5266 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7744 AvaI C'yCGr_G Cuts at: 1870 1943 4971 5069 1870 Size: 73 3028 98 4545 Fragments arranged by size: 4545 3028 98 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3699 3732 4128 4161 3699 Size: 33 396 33 7282 Fragments arranged by size: 7282 396 33 33 BamHI G'GATC_C Cuts at: 5074 5441 5074 Size: 367 7377 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4529 5065 6520 618 Size: 325 217 627 2742 536 1455 1842 Fragments arranged by size: 2742 1842 1455 627 536 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2159 3404 4771 5063 1391 Size: 705 63 1245 1367 292 4072 Fragments arranged by size: 4072 1367 1245 705 292 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2808 4517 4825 928 Size: 971 909 1709 308 3847 Fragments arranged by size: 3847 1709 971 909 308 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5935 5969 1903 Size: 34 3998 34 3678 Fragments arranged by size: 3998 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5636 6846 5636 Size: 1210 6534 BclI T'GATC_A Cuts at: 1548 4539 4951 1548 Size: 2991 412 4341 Fragments arranged by size: 4341 2991 412 BfrBI ATG'CAT Cuts at: 2944 2944 Size: 7744 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6355 136 Size: 107 122 71 631 605 4683 1525 Fragments arranged by size: 4683 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4804 7739 4804 Size: 2935 4809 BlpI GC'TnA_GC Cuts at: 5161 5161 Size: 7744 Bme1580I G_kGCm'C Cuts at: 1790 2781 4482 4758 4771 5805 6961 1790 Size: 991 1701 276 13 1034 1156 2573 Fragments arranged by size: 2573 1701 1156 1034 991 276 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7744 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4524 5475 Size: 416 53 83 399 24 2153 954 951 Cuts at: 5475 6433 442 Size: 958 1753 Fragments arranged by size: 2153 1753 958 954 951 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7744 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3161 3357 4125 4275 4516 6404 1239 Size: 1922 196 768 150 241 1888 2579 Fragments arranged by size: 2579 1922 1888 768 241 196 150 Bpu10I CC'TnA_GC Cuts at: 4418 4418 Size: 7744 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5869 6734 7032 5869 Size: 865 298 6581 Fragments arranged by size: 6581 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2489 6407 882 Size: 617 990 3918 2219 Fragments arranged by size: 3918 2219 990 617 BsaAI yAC'GTr Cuts at: 493 1204 5535 493 Size: 711 4331 2702 Fragments arranged by size: 4331 2702 711 BsaBI GATnn'nnATC Cuts at: 7738 7738 Size: 7744 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5551 5933 6568 275 Size: 53 83 186 936 4018 382 635 1451 Fragments arranged by size: 4018 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4040 6177 6575 6849 6996 7407 4040 Size: 2137 398 274 147 411 4377 Fragments arranged by size: 4377 2137 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2643 2673 3778 3808 3994 Size: 30 165 30 1240 30 1105 30 186 Cuts at: 3994 4024 4273 4303 1178 Size: 30 249 30 4619 Fragments arranged by size: 4619 1240 1105 249 186 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2125 3999 1186 Size: 849 90 1874 4931 Fragments arranged by size: 4931 1874 849 90 BseYI C'CCAG_C Cuts at: 6947 7443 6947 Size: 496 7248 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4215 1163 Size: 573 2479 4692 Fragments arranged by size: 4692 2479 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5194 5955 6983 1540 Size: 275 186 3193 761 1028 2301 Fragments arranged by size: 3193 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2159 2753 2781 3134 3404 4482 4758 5805 5890 Size: 594 28 353 270 1078 276 1047 85 Cuts at: 5890 6961 2159 Size: 1071 2942 Fragments arranged by size: 2942 1078 1071 1047 594 353 276 270 85 28 BsmI GAATG_Cn' Cuts at: 4747 5255 5348 4747 Size: 508 93 7143 Fragments arranged by size: 7143 508 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4793 4793 Size: 7744 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2159 2753 2781 3134 3404 4482 Size: 399 306 63 594 28 353 270 1078 Cuts at: 4482 4758 4771 5063 5805 5890 6961 1391 Size: 276 13 292 742 85 1071 2174 Fragments arranged by size: 2174 1078 1071 742 594 399 353 306 292 276 270 85 63 28 13 BspEI T'CCGG_A Cuts at: 7407 7407 Size: 7744 BspHI T'CATG_A Cuts at: 5633 7363 5633 Size: 1730 6014 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5099 844 Size: 4255 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5631 1394 Size: 429 3808 3507 Fragments arranged by size: 3808 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2873 6239 6413 65 Size: 1183 1625 3366 174 1396 Fragments arranged by size: 3366 1625 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6388 6575 1700 Size: 110 4578 187 2869 Fragments arranged by size: 4578 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4654 96 Size: 4558 3186 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7744 BssSI C'ACGA_G Cuts at: 5804 6816 5804 Size: 1012 6732 BstAPI GCAn_nnn'nTGC Cuts at: 794 2546 794 Size: 1752 5992 BstBI TT'CG_AA Cuts at: 6548 6548 Size: 7744 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7744 BstXI CCAn_nnnn'nTGG Cuts at: 4299 4299 Size: 7744 BstYI r'GATC_y Cuts at: 1972 2314 2974 3302 4346 4629 4804 5001 5074 Size: 342 660 328 1044 283 175 197 73 Cuts at: 5074 5441 5826 5843 7295 7381 7393 7739 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1044 660 385 367 346 342 328 283 197 175 86 73 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2346 Size: 7744 BtgI C'CryG_G Cuts at: 513 1065 2004 4749 513 Size: 552 939 2745 3508 Fragments arranged by size: 3508 2745 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4951 487 Size: 683 56 3725 3280 Fragments arranged by size: 3725 3280 683 56 BtsI GCAGTG_nn' Cuts at: 5260 6054 6074 5260 Size: 794 20 6930 Fragments arranged by size: 6930 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7744 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7709 DraI TTT'AAA Cuts at: 2731 3970 4000 5048 2731 Size: 1239 30 1048 5427 Fragments arranged by size: 5427 1239 1048 30 DraIII CAC_nnn'GTG Cuts at: 4238 4238 Size: 7744 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6751 809 Size: 507 5435 1802 Fragments arranged by size: 5435 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3342 4301 Size: 54 1425 50 275 18 192 1320 959 Cuts at: 4301 5191 6080 7709 8 Size: 890 889 1629 43 Fragments arranged by size: 1629 1425 1320 959 890 889 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5191 1537 Size: 275 3379 4090 Fragments arranged by size: 4090 3379 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2380 2472 2910 4608 5674 1353 Size: 764 263 92 438 1698 1066 3423 Fragments arranged by size: 3423 1698 1066 764 438 263 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 6331 6705 6851 387 Size: 1064 138 435 4307 374 146 1280 Fragments arranged by size: 4307 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2157 3402 2157 Size: 1245 6499 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2361 3161 3357 4125 4275 4278 Size: 393 534 195 800 196 768 150 3 Cuts at: 4278 4289 4516 4609 5807 6404 7191 7459 1239 Size: 11 227 93 1198 597 787 268 1524 Fragments arranged by size: 1524 1198 800 787 768 597 534 393 268 227 196 195 150 93 11 3 EcoNI CCTnn'n_nnAGG Cuts at: 4131 4131 Size: 7744 EcoO109I rG'GnC_Cy Cuts at: 3904 4767 4768 3904 Size: 863 1 6880 Fragments arranged by size: 6880 863 1 EcoRI G'AATT_C Cuts at: 3223 3236 3818 5053 5431 3223 Size: 13 582 1235 378 5536 Fragments arranged by size: 5536 1235 582 378 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3981 3981 Size: 7744 FspI TGC'GCA Cuts at: 6250 6250 Size: 7744 HaeII r_GCGC'y Cuts at: 1678 6891 1678 Size: 5213 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2777 2809 2959 Size: 32 135 32 590 32 583 32 150 Cuts at: 2959 2991 3878 3910 6385 6417 6459 6491 1373 Size: 32 887 32 2475 32 42 32 2626 Fragments arranged by size: 2626 2475 887 590 583 150 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4224 4813 5088 5334 669 Size: 988 281 2286 589 275 246 3079 Fragments arranged by size: 3079 2286 988 589 281 275 246 HindIII A'AGCT_T Cuts at: 748 2825 4722 748 Size: 2077 1897 3770 Fragments arranged by size: 3770 2077 1897 HpaI GTT'AAC Cuts at: 5334 5334 Size: 7744 KpnI G_GTAC'C Cuts at: 5069 5069 Size: 7744 MfeI C'AATT_G Cuts at: 4989 5343 4989 Size: 354 7390 MluI A'CGCG_T Cuts at: 7620 7620 Size: 7744 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6858 7042 1305 Size: 5553 184 2007 Fragments arranged by size: 5553 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3344 4303 10 Size: 54 1425 343 1512 959 3451 Fragments arranged by size: 3451 1512 1425 959 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2901 3885 4032 4577 5702 6061 6220 Size: 648 1735 984 147 545 1125 359 159 Cuts at: 6220 518 Size: 2042 Fragments arranged by size: 2042 1735 1125 984 648 545 359 159 147 MspA1I CmG'CkG Cuts at: 1606 5160 5837 6985 7230 1606 Size: 3554 677 1148 245 2120 Fragments arranged by size: 3554 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4749 513 Size: 552 939 2745 3508 Fragments arranged by size: 3508 2745 939 552 NdeI CA'TA_TG Cuts at: 387 4315 387 Size: 3928 3816 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7744 NotI GC'GGCC_GC Cuts at: 5191 5191 Size: 7744 NsiI A_TGCA'T Cuts at: 2946 2946 Size: 7744 NspI r_CATG'y Cuts at: 1132 1229 3693 4448 5102 6647 1132 Size: 97 2464 755 654 1545 2229 Fragments arranged by size: 2464 2229 1545 755 654 97 PasI CC'CwG_GG Cuts at: 1348 2113 1348 Size: 765 6979 PciI A'CATG_T Cuts at: 6643 6643 Size: 7744 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7744 PmeI GTTT'AAAC Cuts at: 5048 5048 Size: 7744 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5813 5845 7326 7358 5813 Size: 32 1481 32 6199 Fragments arranged by size: 6199 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3904 3904 Size: 7744 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7744 PsiI TTA'TAA Cuts at: 5314 5314 Size: 7744 PspOMI G'GGCC_C Cuts at: 4767 4767 Size: 7744 PstI C_TGCA'G Cuts at: 830 2132 5096 830 Size: 1302 2964 3478 Fragments arranged by size: 3478 2964 1302 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7744 SacI G_AGCT'C Cuts at: 2159 3404 2159 Size: 1245 6499 SalI G'TCGA_C Cuts at: 1936 5086 1936 Size: 3150 4594 SapI GCTCTTCn'nnn_ Cuts at: 2117 2472 4608 2117 Size: 355 2136 5253 Fragments arranged by size: 5253 2136 355 SbfI CC_TGCA'GG Cuts at: 5096 5096 Size: 7744 ScaI AGT'ACT Cuts at: 2597 4266 5992 7704 2597 Size: 1669 1726 1712 2637 Fragments arranged by size: 2637 1726 1712 1669 SfcI C'TryA_G Cuts at: 826 1045 2128 3033 4014 5092 6227 6908 7099 Size: 219 1083 905 981 1078 1135 681 191 Cuts at: 7099 826 Size: 1471 Fragments arranged by size: 1471 1135 1083 1078 981 905 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7744 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7744 SmaI CCC'GGG Cuts at: 1872 5071 1872 Size: 3199 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5848 6749 7011 820 Size: 197 926 3905 901 262 1553 Fragments arranged by size: 3905 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5535 493 Size: 5042 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7744 SphI G_CATG'C Cuts at: 1132 5102 1132 Size: 3970 3774 SspI AAT'ATT Cuts at: 5 52 5668 5 Size: 47 5616 2081 Fragments arranged by size: 5616 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7744 StyI C'CwwG_G Cuts at: 513 1065 2004 4132 4749 5183 513 Size: 552 939 2128 617 434 3074 Fragments arranged by size: 3074 2128 939 617 552 434 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 5771 5788 5941 6126 6571 325 Size: 1255 1437 2754 17 153 185 445 1498 Fragments arranged by size: 2754 1498 1437 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 4264 4654 Size: 274 80 33 51 1443 618 1669 390 Cuts at: 4654 4935 5990 7702 96 Size: 281 1055 1712 138 Fragments arranged by size: 1712 1669 1443 1055 618 390 281 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4273 4305 7704 7736 4273 Size: 32 3399 32 4281 Fragments arranged by size: 4281 3399 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4984 1316 Size: 3668 4076 XbaI T'CTAG_A Cuts at: 2320 5080 2320 Size: 2760 4984 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3352 3352 Size: 7744 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7744 XmaI C'CCGG_G Cuts at: 1870 5069 1870 Size: 3199 4545 XmnI GAAnn'nnTTC Cuts at: 5873 5873 Size: 7744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5552 276 Size: 53 83 186 936 4018 2468 Fragments arranged by size: 4018 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI