(Linear) (Six-base) MAPSORT of: KIBB9889.seq Check: 9603 from: 1 to: 3129 KIBB9889.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AccI GT'mk_AC Cuts at: 0 2180 3129 Size: 2180 949 AclI AA'CG_TT Cuts at: 0 2274 3129 Size: 2274 855 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 661 1597 2097 2689 3129 Size: 146 515 936 500 592 440 Fragments arranged by size: 936 592 515 500 440 146 AleI CACnn'nnGTG Cuts at: 0 1786 3129 Size: 1786 1343 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1101 3129 Size: 1101 2028 ApaLI G'TGCA_C Cuts at: 0 1159 3129 Size: 1159 1970 ApoI r'AATT_y Cuts at: 0 50 70 85 268 345 1053 3124 3129 Size: 50 20 15 183 77 708 2071 5 Fragments arranged by size: 2071 708 183 77 50 20 15 5 AseI AT'TA_AT Cuts at: 0 1166 2068 3129 Size: 1166 902 1061 Fragments arranged by size: 1166 1061 902 AvaI C'yCGr_G Cuts at: 0 588 2161 3129 Size: 588 1573 968 Fragments arranged by size: 1573 968 588 AvrII C'CTAG_G Cuts at: 0 411 3129 Size: 411 2718 BanI G'GyrC_C Cuts at: 0 611 2119 3129 Size: 611 1508 1010 Fragments arranged by size: 1508 1010 611 BanII G_rGCy'C Cuts at: 0 759 1218 1241 1387 2765 3129 Size: 759 459 23 146 1378 364 Fragments arranged by size: 1378 759 459 364 146 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 667 1580 1730 2226 3129 Size: 667 913 150 496 903 Fragments arranged by size: 913 903 667 496 150 BbvCI CC'TCA_GC Cuts at: 0 1231 1435 3129 Size: 1231 204 1694 Fragments arranged by size: 1694 1231 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 311 345 983 1017 1498 1532 2678 2712 Size: 311 34 638 34 481 34 1146 34 Cuts at: 2712 3129 Size: 417 Fragments arranged by size: 1146 638 481 417 311 34 34 34 34 BfrBI ATG'CAT Cuts at: 0 3031 3129 Size: 3031 98 BglII A'GATC_T Cuts at: 0 881 2426 3129 Size: 881 1545 703 Fragments arranged by size: 1545 881 703 BlpI GC'TnA_GC Cuts at: 0 694 2759 3129 Size: 694 2065 370 Fragments arranged by size: 2065 694 370 Bme1580I G_kGCm'C Cuts at: 0 570 1163 2122 2716 3129 Size: 570 593 959 594 413 Fragments arranged by size: 959 594 593 570 413 BmgBI CAC'GTC Cuts at: 0 1821 3129 Size: 1821 1308 BmrI ACTGGGnnnn_n' Cuts at: 0 1648 3129 Size: 1648 1481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 773 2107 3129 Size: 773 1334 1022 Fragments arranged by size: 1334 1022 773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 196 558 1039 1381 2989 3129 Size: 170 26 362 481 342 1608 140 Fragments arranged by size: 1608 481 362 342 170 140 26 Bpu10I CC'TnA_GC Cuts at: 0 758 1231 1435 3072 3129 Size: 758 473 204 1637 57 Fragments arranged by size: 1637 758 473 204 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 1113 1225 2095 3129 Size: 646 467 112 870 1034 Fragments arranged by size: 1034 870 646 467 112 BsaI GGTCTCn'nnnn_ Cuts at: 0 907 2349 3129 Size: 907 1442 780 Fragments arranged by size: 1442 907 780 BsaWI w'CCGG_w Cuts at: 0 476 2093 2519 2921 3129 Size: 476 1617 426 402 208 Fragments arranged by size: 1617 476 426 402 208 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1108 2641 3129 Size: 1108 1533 488 Fragments arranged by size: 1533 1108 488 BseYI C'CCAG_C Cuts at: 0 2079 3129 Size: 2079 1050 BsiHKAI G_wGCw'C Cuts at: 0 368 759 857 1163 1218 1241 1343 2765 Size: 368 391 98 306 55 23 102 1422 Cuts at: 2765 3129 Size: 364 Fragments arranged by size: 1422 391 368 364 306 102 98 55 23 BsmI GAATG_Cn' Cuts at: 0 947 1527 2087 2937 3024 3129 Size: 947 580 560 850 87 105 Fragments arranged by size: 947 850 580 560 105 87 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1811 1999 3129 Size: 1811 188 1130 Fragments arranged by size: 1811 1130 188 Bsp1286I G_dGCh'C Cuts at: 0 368 570 759 857 1163 1218 1241 1343 Size: 368 202 189 98 306 55 23 102 Cuts at: 1343 1387 2122 2716 2765 3129 Size: 44 735 594 49 364 Fragments arranged by size: 735 594 368 364 306 202 189 102 98 55 49 44 23 BspEI T'CCGG_A Cuts at: 0 476 2093 2519 2921 3129 Size: 476 1617 426 402 208 Fragments arranged by size: 1617 476 426 402 208 BspHI T'CATG_A Cuts at: 0 785 1886 2546 2579 3129 Size: 785 1101 660 33 550 Fragments arranged by size: 1101 785 660 550 33 BsrDI GCAATG_nn' Cuts at: 0 461 1095 1664 2033 2412 3129 Size: 461 634 569 369 379 717 Fragments arranged by size: 717 634 569 461 379 369 BsrFI r'CCGG_y Cuts at: 0 1178 1226 2122 3129 Size: 1178 48 896 1007 Fragments arranged by size: 1178 1007 896 48 BsrGI T'GTAC_A Cuts at: 0 1460 2987 3129 Size: 1460 1527 142 Fragments arranged by size: 1527 1460 142 BssSI C'ACGA_G Cuts at: 0 2002 2635 3129 Size: 2002 633 494 Fragments arranged by size: 2002 633 494 BstXI CCAn_nnnn'nTGG Cuts at: 0 2211 3129 Size: 2211 918 BstYI r'GATC_y Cuts at: 0 106 881 1744 2426 2658 3129 Size: 106 775 863 682 232 471 Fragments arranged by size: 863 775 682 471 232 106 Bsu36I CC'TnA_GG Cuts at: 0 3062 3129 Size: 3062 67 BtgI C'CryG_G Cuts at: 0 387 602 3129 Size: 387 215 2527 Fragments arranged by size: 2527 387 215 BtsI GCAGTG_nn' Cuts at: 0 570 644 845 2067 3129 Size: 570 74 201 1222 1062 Fragments arranged by size: 1222 1062 570 201 74 ClaI AT'CG_AT Cuts at: 0 482 3129 Size: 482 2647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 5 40 3129 Size: 5 35 3089 Fragments arranged by size: 3089 35 5 DraIII CAC_nnn'GTG Cuts at: 0 861 3129 Size: 861 2268 DrdI GACnn_nn'nnGTC Cuts at: 0 737 3129 Size: 737 2392 EaeI y'GGCC_r Cuts at: 0 451 1412 3129 Size: 451 961 1717 Fragments arranged by size: 1717 961 451 EarI CTCTTCn'nnn_ Cuts at: 0 664 747 2050 3129 Size: 664 83 1303 1079 Fragments arranged by size: 1303 1079 664 83 EcoICRI GAG'CTC Cuts at: 0 757 1216 1239 2763 3129 Size: 757 459 23 1524 366 Fragments arranged by size: 1524 757 459 366 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 170 196 558 661 1039 1381 1597 Size: 146 24 26 362 103 378 342 216 Cuts at: 1597 2097 2689 2989 3129 Size: 500 592 300 140 Fragments arranged by size: 592 500 378 362 342 300 216 146 140 103 26 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 199 3129 Size: 199 2930 EcoO109I rG'GnC_Cy Cuts at: 0 3078 3129 Size: 3078 51 EcoRI G'AATT_C Cuts at: 0 345 1053 3129 Size: 345 708 2076 Fragments arranged by size: 2076 708 345 EcoRV GAT'ATC Cuts at: 0 379 997 3129 Size: 379 618 2132 Fragments arranged by size: 2132 618 379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 171 334 948 2127 3129 Size: 171 163 614 1179 1002 Fragments arranged by size: 1179 1002 614 171 163 FseI GG_CCGG'CC Cuts at: 0 1182 3129 Size: 1182 1947 HaeII r_GCGC'y Cuts at: 0 2919 3129 Size: 2919 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 368 400 487 519 657 689 1276 1308 Size: 368 32 87 32 138 32 587 32 Cuts at: 1308 1606 1638 3129 Size: 298 32 1491 Fragments arranged by size: 1491 587 368 298 138 87 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 501 1425 3129 Size: 501 924 1704 Fragments arranged by size: 1704 924 501 HindIII A'AGCT_T Cuts at: 0 3069 3129 Size: 3069 60 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2318 3129 Size: 2318 811 MscI TGG'CCA Cuts at: 0 1414 3129 Size: 1414 1715 MslI CAynn'nnrTG Cuts at: 0 1786 1838 1885 2551 3129 Size: 1786 52 47 666 578 Fragments arranged by size: 1786 666 578 52 47 MspA1I CmG'CkG Cuts at: 0 1402 1439 2413 3129 Size: 1402 37 974 716 Fragments arranged by size: 1402 974 716 37 NaeI GCC'GGC Cuts at: 0 1180 3129 Size: 1180 1949 NcoI C'CATG_G Cuts at: 0 387 602 3129 Size: 387 215 2527 Fragments arranged by size: 2527 387 215 NgoMIV G'CCGG_C Cuts at: 0 1178 3129 Size: 1178 1951 NsiI A_TGCA'T Cuts at: 0 3033 3129 Size: 3033 96 NspI r_CATG'y Cuts at: 0 931 1513 3129 Size: 931 582 1616 Fragments arranged by size: 1616 931 582 PflMI CCAn_nnn'nTGG Cuts at: 0 388 608 3129 Size: 388 220 2521 Fragments arranged by size: 2521 388 220 PfoI T'CCnGG_A Cuts at: 0 194 3129 Size: 194 2935 PpuMI rG'GwC_Cy Cuts at: 0 3078 3129 Size: 3078 51 PspXI vC'TCGA_Gb Cuts at: 0 2161 3129 Size: 2161 968 PstI C_TGCA'G Cuts at: 0 808 1397 1444 3129 Size: 808 589 47 1685 Fragments arranged by size: 1685 808 589 47 PvuII CAG'CTG Cuts at: 0 1402 1439 2413 3129 Size: 1402 37 974 716 Fragments arranged by size: 1402 974 716 37 SacI G_AGCT'C Cuts at: 0 759 1218 1241 2765 3129 Size: 759 459 23 1524 364 Fragments arranged by size: 1524 759 459 364 23 SanDI GG'GwC_CC Cuts at: 0 3078 3129 Size: 3078 51 SapI GCTCTTCn'nnn_ Cuts at: 0 747 2050 3129 Size: 747 1303 1079 Fragments arranged by size: 1303 1079 747 SfcI C'TryA_G Cuts at: 0 804 1393 1440 1847 3129 Size: 804 589 47 407 1282 Fragments arranged by size: 1282 804 589 407 47 SmlI C'TyrA_G Cuts at: 0 661 1128 1240 2074 2161 3129 Size: 661 467 112 834 87 968 Fragments arranged by size: 968 834 661 467 112 87 SphI G_CATG'C Cuts at: 0 1513 3129 Size: 1513 1616 StuI AGG'CCT Cuts at: 0 416 2159 3129 Size: 416 1743 970 Fragments arranged by size: 1743 970 416 StyI C'CwwG_G Cuts at: 0 23 387 411 467 602 3129 Size: 23 364 24 56 135 2527 Fragments arranged by size: 2527 364 135 56 24 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 33 74 3129 Size: 33 41 3055 Fragments arranged by size: 3055 41 33 TatI w'GTAC_w Cuts at: 0 1460 2188 2732 2987 3129 Size: 1460 728 544 255 142 Fragments arranged by size: 1460 728 544 255 142 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 610 642 3129 Size: 610 32 2487 Fragments arranged by size: 2487 610 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2398 2592 3129 Size: 2398 194 537 Fragments arranged by size: 2398 537 194 XhoI C'TCGA_G Cuts at: 0 2161 3129 Size: 2161 968 XmnI GAAnn'nnTTC Cuts at: 0 893 3129 Size: 893 2236 Enzymes that do cut: AccI AclI AcuI AleI AlfI ApaLI ApoI AseI AvaI AvrII BanI BanII BbsI BbvCI BcgI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PpuMI PspXI PstI PvuII SacI SanDI SapI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaI AscI AsiSI BaeI BamHI BbeI BciVI BclI BglI BmtI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BspMI BsrBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI DraI EagI EciI FspI FspAI HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI ZraI