(Circular) (Six-base) MAPSORT of: pFN21AE3184.seq Check: 106 from: 1 to: 5971 pFN21AE3184 5971 bp With 182 enzymes: SgfI * March 28, 2012 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2419 4034 5418 5686 1632 Size: 787 1615 1384 268 1917 Fragments arranged by size: 1917 1615 1384 787 268 AflII C'TTAA_G Cuts at: 820 1017 2726 820 Size: 197 1709 4065 Fragments arranged by size: 4065 1709 197 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 2354 1166 Size: 1188 4783 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2550 2582 2550 Size: 32 5939 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaLI G'TGCA_C Cuts at: 3115 4028 5184 3115 Size: 913 1156 3902 Fragments arranged by size: 3902 1156 913 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 AvrII C'CTAG_G Cuts at: 3233 3233 Size: 5971 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2903 3149 3290 1391 Size: 670 842 246 141 4072 Fragments arranged by size: 4072 842 670 246 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 2358 1548 Size: 810 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2869 3092 3119 4032 5188 1790 Size: 1079 223 27 913 1156 2573 Fragments arranged by size: 2573 1156 1079 913 223 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2474 2872 3702 Size: 416 53 83 399 24 1057 398 830 Cuts at: 3702 4660 442 Size: 958 1753 Fragments arranged by size: 1753 1057 958 830 416 399 398 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4631 1239 Size: 3392 2579 Bpu10I CC'TnA_GC Cuts at: 2142 2142 Size: 5971 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2465 2776 2798 4634 882 Size: 617 966 311 22 1836 2219 Fragments arranged by size: 2219 1836 966 617 311 22 BsaAI yAC'GTr Cuts at: 493 1204 3082 3762 493 Size: 711 1878 680 2702 Fragments arranged by size: 2702 1878 711 680 BsaBI GATnn'nnATC Cuts at: 2638 5965 2638 Size: 3327 2644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2658 3778 4160 4795 Size: 53 83 186 936 1125 1120 382 635 Cuts at: 4795 275 Size: 1451 Fragments arranged by size: 1451 1125 1120 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 1178 Size: 30 165 30 1147 30 4569 Fragments arranged by size: 4569 1147 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2388 2816 3182 1186 Size: 1202 428 366 3975 Fragments arranged by size: 3975 1202 428 366 BseYI C'CCAG_C Cuts at: 2054 5174 5670 2054 Size: 3120 496 2355 Fragments arranged by size: 3120 2355 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2942 3062 1163 Size: 573 1206 120 4072 Fragments arranged by size: 4072 1206 573 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3119 4032 4117 5188 3119 Size: 913 85 1071 3902 Fragments arranged by size: 3902 1071 913 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2869 2903 3092 3119 3149 3290 Size: 399 271 808 34 189 27 30 141 Cuts at: 3290 4032 4117 5188 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 808 742 399 271 189 141 85 34 30 27 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2625 4466 4640 65 Size: 1183 1377 1841 174 1396 Fragments arranged by size: 1841 1396 1377 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2624 4615 4802 1700 Size: 110 814 1991 187 2869 Fragments arranged by size: 2869 1991 814 187 110 BsrGI T'GTAC_A Cuts at: 96 2812 2829 96 Size: 2716 17 3238 Fragments arranged by size: 3238 2716 17 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 2029 4775 2029 Size: 2746 3225 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2639 3301 3668 4053 4070 5522 5608 5620 Size: 667 662 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 667 662 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2117 2117 Size: 5971 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3194 487 Size: 683 56 1968 3264 Fragments arranged by size: 3264 1968 683 56 BtsI GCAGTG_nn' Cuts at: 2384 3487 4281 4301 2384 Size: 1103 794 20 4054 Fragments arranged by size: 4054 1103 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 2020 3275 2020 Size: 1255 4716 DraIII CAC_nnn'GTG Cuts at: 3096 3096 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3233 3901 1353 Size: 1880 668 3423 Fragments arranged by size: 3423 1880 668 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2419 4034 4631 5418 5686 1239 Size: 393 787 1615 597 787 268 1524 Fragments arranged by size: 1615 1524 787 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2771 2771 Size: 5971 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2841 2841 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4612 4644 4686 4718 1373 Size: 32 135 32 3040 32 42 32 2626 Fragments arranged by size: 3040 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2545 5085 5269 1305 Size: 1240 2540 184 2007 Fragments arranged by size: 2540 2007 1240 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2285 2354 3929 4288 4447 518 Size: 648 843 276 69 1575 359 159 2042 Fragments arranged by size: 2042 1575 843 648 359 276 159 69 MspA1I CmG'CkG Cuts at: 1606 2054 2926 3387 4064 5212 5457 1606 Size: 448 872 461 677 1148 245 2120 Fragments arranged by size: 2120 1148 872 677 461 448 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PfoI T'CCnGG_A Cuts at: 2331 3129 2331 Size: 798 5173 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PmlI CAC'GTG Cuts at: 3082 3082 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2550 2582 3237 3269 4040 4072 5553 5585 2550 Size: 32 655 32 771 32 1481 32 2936 Fragments arranged by size: 2936 1481 771 655 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2771 2771 Size: 5971 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3041 3541 3041 Size: 500 5471 PstI C_TGCA'G Cuts at: 830 2215 2391 3219 3323 830 Size: 1385 176 828 104 3478 Fragments arranged by size: 3478 1385 828 176 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2054 2926 2054 Size: 872 5099 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 2675 4219 5931 2675 Size: 1544 1712 2715 Fragments arranged by size: 2715 1712 1544 SfcI C'TryA_G Cuts at: 826 1045 2049 2211 2387 2775 3045 3215 3319 Size: 219 1004 162 176 388 270 170 104 Cuts at: 3319 4454 5135 5326 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1004 681 388 270 219 191 176 170 162 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 4075 4976 5238 820 Size: 197 926 783 1349 901 262 1553 Fragments arranged by size: 1553 1349 926 901 783 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 2841 3895 5 Size: 47 2789 1054 2081 Fragments arranged by size: 2789 2081 1054 47 StuI AGG'CCT Cuts at: 1517 2821 1517 Size: 1304 4667 StyI C'CwwG_G Cuts at: 513 1065 3233 3410 513 Size: 552 2168 177 3074 Fragments arranged by size: 3074 2168 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3281 3998 4015 4168 4353 4798 325 Size: 1255 1701 717 17 153 185 445 1498 Fragments arranged by size: 1701 1498 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 2812 2829 Size: 274 80 33 51 1443 696 139 17 Cuts at: 2829 4217 5929 96 Size: 1388 1712 138 Fragments arranged by size: 1712 1443 1388 696 274 139 138 80 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2090 2329 3079 2090 Size: 239 750 4982 Fragments arranged by size: 4982 750 239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 2991 4100 2991 Size: 1109 4862 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI