################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BbsI BcgI BciVI BclI BfrI BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 867 GTCTAC GT^[AC][GT]AC AccII 6 CGCG CG^CG AcsI 1622 AAATTT [GA]^AATT[CT] Alw44I 1456 GTGCAC G^TGCAC AocI 262 CCTCAGG CC^T[ACGT]AGG Aor51HI 693 AGCGCT AGC^GCT ApaI 158 GGGCCC GGGCC^C ApaLI 1456 GTGCAC G^TGCAC ApoI 1622 AAATTT [GA]^AATT[CT] AvaII 906 GGACC G^G[AT]CC BalI 1484 TGGCCA TGG^CCA BamHI 61 GGATCC G^GATCC BbeI 148 GGCGCC GGCGC^C BfaI 481 CTAG C^TAG BglII 44 AGATCT A^GATCT BlpI 120 GCTGAGC GC^T[ACGT]AGC Bpu1102I 120 GCTGAGC GC^T[ACGT]AGC BsaAI 1491 CACGTA [CT]AC^GT[GA] BsaI 352 GAGACC GGTCTC(1/5) BsgI 422 CTGCAC GTGCAG(16/14) BsmAI 352 GAGAC GTCTC(1/5) BsrBI 1050 CCGCTC CCGCTC(-3/-3) BstUI 6 CGCG CG^CG Bsu36I 262 CCTCAGG CC^T[ACGT]AGG CelII 120 GCTGAGC GC^T[ACGT]AGC DraIII 441 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 300 GACCGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 693 AGCGCT AGC^GCT Eco81I 262 CCTCAGG CC^T[ACGT]AGG HgaI 990 GACGC GACGC(5/10) KasI 148 GGCGCC G^GCGCC KspI 5 CCGCGG CCGC^GG MaeI 481 CTAG C^TAG MaeII 1492 ACGT A^CGT MfeI 1616 CAATTG C^AATTG MluNI 1484 TGGCCA TGG^CCA MscI 1484 TGGCCA TGG^CCA MseI 875 TTAA T^TAA MslI 1113 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1616 CAATTG C^AATTG MvnI 6 CGCG CG^CG NarI 148 GGCGCC GG^CGCC NcoI 83 CCATGG C^CATGG PflMI 822 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 905 AGGACCT [GA]G^G[AT]CC[CT] PshAI 592 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 40 CTGCAG CTGCA^G SacI 1060 GAGCTC GAGCT^C SacII 5 CCGCGG CCGC^GG SfcI 40 CTGCAG C^T[GA][CT]AG SfiI 1293 GGCCTCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 148 GGCGCC GGC^GCC SmaI 675 CCCGGG CCC^GGG Tru9I 875 TTAA T^TAA Tsp45I 1552 GTCAC ^GT[GC]AC Van91I 822 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 675 CCCGGG C^CCGGG XmaI 675 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 148 GGCGCC G[GA]^CG[CT]C 990 GACGCC AlwNI 823 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 904 CAGGACCTG Asp700I 813 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1095 GAAGCCCTTC AvaI 451 CCCGAG C^[CT]CG[GA]G 675 CCCGGG BbvCI 515 CCTCAGC CCTCAGC(-5/-2) 1544 CCTCAGC BglI 151 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1294 GCCTCTTCGGC BsaHI 148 GGCGCC G[GA]^CG[CT]C 990 GACGCC BsoBI 451 CCCGAG C^[CT]CG[GA]G 675 CCCGGG BsrFI 501 ACCGGC [GA]^CCGG[CT] 977 ACCGGC Cfr10I 501 ACCGGC [GA]^CCGG[CT] 977 ACCGGC DsaI 5 CCGCGG C^C[GA][CT]GG 83 CCATGG Hin1I 148 GGCGCC G[GA]^CG[CT]C 990 GACGCC HinfI 307 GAGTC G^A[ACGT]TC 1027 GAGTC NspI 959 GCATGC [GA]CATG^[CT] 1118 GCATGC PleI 307 GAGTC GAGTC(4/5) 1027 GAGTC PvuII 25 CAGCTG CAG^CTG 1547 CAGCTG SphI 959 GCATGC GCATG^C 1118 GCATGC StuI 668 AGGCCT AGG^CCT 878 AGGCCT TaqI 378 TCGA T^CGA 1438 TCGA Tsp509I 1617 AATT ^AATT 1623 AATT TthHB8I 378 TCGA T^CGA 1438 TCGA XmnI 813 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1095 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AviII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI XhoII