(Linear) (Six-base) MAPSORT of: KIBB7053.seq Check: 9857 from: 1 to: 1629 KIBB7053.orf With 182 enzymes: SgfI * April 19, 2010 16:26 .. AccI GT'mk_AC Cuts at: 0 868 1629 Size: 868 761 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 447 931 1113 1548 1629 Size: 447 484 182 435 81 Fragments arranged by size: 484 447 435 182 81 AfeI AGC'GCT Cuts at: 0 695 1629 Size: 695 934 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1135 1629 Size: 1135 494 AlwNI CAG_nnn'CTG Cuts at: 0 828 909 1629 Size: 828 81 720 Fragments arranged by size: 828 720 81 ApaI G_GGCC'C Cuts at: 0 162 1629 Size: 162 1467 ApaLI G'TGCA_C Cuts at: 0 1456 1629 Size: 1456 173 ApoI r'AATT_y Cuts at: 0 1622 1629 Size: 1622 7 AvaI C'yCGr_G Cuts at: 0 451 675 1629 Size: 451 224 954 Fragments arranged by size: 954 451 224 BamHI G'GATC_C Cuts at: 0 61 1629 Size: 61 1568 BanI G'GyrC_C Cuts at: 0 148 615 799 1629 Size: 148 467 184 830 Fragments arranged by size: 830 467 184 148 BanII G_rGCy'C Cuts at: 0 135 162 458 562 682 901 1064 1629 Size: 135 27 296 104 120 219 163 565 Fragments arranged by size: 565 296 219 163 135 120 104 27 BbeI G_GCGC'C Cuts at: 0 152 1629 Size: 152 1477 BbvCI CC'TCA_GC Cuts at: 0 516 1545 1629 Size: 516 1029 84 Fragments arranged by size: 1029 516 84 BglI GCCn_nnn'nGGC Cuts at: 0 157 1300 1629 Size: 157 1143 329 Fragments arranged by size: 1143 329 157 BglII A'GATC_T Cuts at: 0 44 1629 Size: 44 1585 BlpI GC'TnA_GC Cuts at: 0 121 1629 Size: 121 1508 Bme1580I G_kGCm'C Cuts at: 0 162 802 1412 1460 1629 Size: 162 640 610 48 169 Fragments arranged by size: 640 610 169 162 48 BmrI ACTGGGnnnn_n' Cuts at: 0 132 1015 1629 Size: 132 883 614 Fragments arranged by size: 883 614 132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 436 512 682 850 1629 Size: 124 312 76 170 168 779 Fragments arranged by size: 779 312 170 168 124 76 Bpu10I CC'TnA_GC Cuts at: 0 98 516 568 653 1545 1629 Size: 98 418 52 85 892 84 Fragments arranged by size: 892 418 98 85 84 52 BsaI GGTCTCn'nnnn_ Cuts at: 0 346 1629 Size: 346 1283 BsaAI yAC'GTr Cuts at: 0 1493 1629 Size: 1493 136 BsaHI Gr'CG_yC Cuts at: 0 149 991 1629 Size: 149 842 638 Fragments arranged by size: 842 638 149 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1590 1620 1629 Size: 1590 30 9 Fragments arranged by size: 1590 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 498 528 1299 1413 1425 1488 1629 Size: 121 377 30 771 114 12 63 141 Fragments arranged by size: 771 377 141 121 114 63 30 12 BseYI C'CCAG_C Cuts at: 0 364 842 938 952 982 1629 Size: 364 478 96 14 30 647 Fragments arranged by size: 647 478 364 96 30 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 407 1629 Size: 407 1222 BsiHKAI G_wGCw'C Cuts at: 0 296 1064 1460 1629 Size: 296 768 396 169 Fragments arranged by size: 768 396 296 169 Bsp1286I G_dGCh'C Cuts at: 0 135 162 296 458 562 682 802 901 Size: 135 27 134 162 104 120 120 99 Cuts at: 901 1064 1412 1460 1629 Size: 163 348 48 169 Fragments arranged by size: 348 169 163 162 135 134 120 120 104 99 48 27 BsrBI CCG'CTC Cuts at: 0 1052 1629 Size: 1052 577 BsrFI r'CCGG_y Cuts at: 0 501 977 1629 Size: 501 476 652 Fragments arranged by size: 652 501 476 BstYI r'GATC_y Cuts at: 0 44 61 786 1247 1629 Size: 44 17 725 461 382 Fragments arranged by size: 725 461 382 44 17 Bsu36I CC'TnA_GG Cuts at: 0 263 1629 Size: 263 1366 BtgI C'CryG_G Cuts at: 0 5 83 1629 Size: 5 78 1546 Fragments arranged by size: 1546 78 5 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1238 1629 Size: 1238 391 BtsI GCAGTG_nn' Cuts at: 0 584 1629 Size: 584 1045 DraIII CAC_nnn'GTG Cuts at: 0 446 1629 Size: 446 1183 DrdI GACnn_nn'nnGTC Cuts at: 0 306 1629 Size: 306 1323 EaeI y'GGCC_r Cuts at: 0 2 8 402 1301 1382 1484 1629 Size: 2 6 394 899 81 102 145 Fragments arranged by size: 899 394 145 102 81 6 2 EarI CTCTTCn'nnn_ Cuts at: 0 466 1302 1323 1629 Size: 466 836 21 306 Fragments arranged by size: 836 466 306 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 283 531 537 1629 Size: 283 248 6 1092 Fragments arranged by size: 1092 283 248 6 EcoICRI GAG'CTC Cuts at: 0 1062 1629 Size: 1062 567 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 436 447 512 682 850 931 1113 Size: 124 312 11 65 170 168 81 182 Cuts at: 1113 1548 1629 Size: 435 81 Fragments arranged by size: 435 312 182 170 168 124 81 81 65 11 EcoO109I rG'GnC_Cy Cuts at: 0 158 159 825 906 915 1629 Size: 158 1 666 81 9 714 Fragments arranged by size: 714 666 158 81 9 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 753 1269 1629 Size: 753 516 360 Fragments arranged by size: 753 516 360 FspI TGC'GCA Cuts at: 0 576 964 1123 1629 Size: 576 388 159 506 Fragments arranged by size: 576 506 388 159 HaeII r_GCGC'y Cuts at: 0 152 697 1571 1629 Size: 152 545 874 58 Fragments arranged by size: 874 545 152 58 KasI G'GCGC_C Cuts at: 0 148 1629 Size: 148 1481 MfeI C'AATT_G Cuts at: 0 1616 1629 Size: 1616 13 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 611 1079 1629 Size: 611 468 550 Fragments arranged by size: 611 550 468 MscI TGG'CCA Cuts at: 0 1486 1629 Size: 1486 143 MslI CAynn'nnrTG Cuts at: 0 1117 1629 Size: 1117 512 MspA1I CmG'CkG Cuts at: 0 7 27 39 173 200 532 613 1549 Size: 7 20 12 134 27 332 81 936 Cuts at: 1549 1629 Size: 80 Fragments arranged by size: 936 332 134 81 80 27 20 12 7 NarI GG'CG_CC Cuts at: 0 149 1629 Size: 149 1480 NcoI C'CATG_G Cuts at: 0 83 1629 Size: 83 1546 NspI r_CATG'y Cuts at: 0 963 1122 1629 Size: 963 159 507 Fragments arranged by size: 963 507 159 PflMI CCAn_nnn'nTGG Cuts at: 0 828 1629 Size: 828 801 PpuMI rG'GwC_Cy Cuts at: 0 906 1629 Size: 906 723 PshAI GACnn'nnGTC Cuts at: 0 596 1629 Size: 596 1033 PspOMI G'GGCC_C Cuts at: 0 158 1629 Size: 158 1471 PstI C_TGCA'G Cuts at: 0 44 1629 Size: 44 1585 PvuII CAG'CTG Cuts at: 0 27 1549 1629 Size: 27 1522 80 Fragments arranged by size: 1522 80 27 SacI G_AGCT'C Cuts at: 0 1064 1629 Size: 1064 565 SacII CC_GC'GG Cuts at: 0 8 1629 Size: 8 1621 SfcI C'TryA_G Cuts at: 0 40 1629 Size: 40 1589 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1300 1629 Size: 1300 329 SfoI GGC'GCC Cuts at: 0 150 1629 Size: 150 1479 SmaI CCC'GGG Cuts at: 0 677 1629 Size: 677 952 SphI G_CATG'C Cuts at: 0 963 1122 1629 Size: 963 159 507 Fragments arranged by size: 963 507 159 StuI AGG'CCT Cuts at: 0 670 880 1629 Size: 670 210 749 Fragments arranged by size: 749 670 210 StyI C'CwwG_G Cuts at: 0 11 83 728 1629 Size: 11 72 645 901 Fragments arranged by size: 901 645 72 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 441 633 856 1168 1180 1629 Size: 441 192 223 312 12 449 Fragments arranged by size: 449 441 312 223 192 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 608 640 1629 Size: 608 32 989 Fragments arranged by size: 989 608 32 XmaI C'CCGG_G Cuts at: 0 675 1629 Size: 675 954 XmnI GAAnn'nnTTC Cuts at: 0 817 1099 1629 Size: 817 282 530 Fragments arranged by size: 817 530 282 Enzymes that do cut: AccI AcuI AfeI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PpuMI PshAI PspOMI PstI PvuII SacI SacII SfcI SfiI SfoI SmaI SphI StuI StyI TaqII TstI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EagI EcoNI EcoRI EcoRV FseI FspAI Hin4I HincII HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI ZraI