################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DdeI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HindIII KasI KpnI Ksp632I KspI MaeI MamI MflI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 377 TCCGGA T^CCGGA AcsI 373 GAATTC [GA]^AATT[CT] ApoI 373 GAATTC [GA]^AATT[CT] Asp700I 369 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 39 GGTCC G^G[AT]CC AviII 417 TGCGCA TGC^GCA BalI 317 TGGCCA TGG^CCA BbvI 141 GCAGC GCAGC(8/12) BcnI 46 CCCGG CC^[GC]GG BpmI 229 CTGGAG CTGGAG(16/14) BsaWI 377 TCCGGA [AT]^CCGG[AT] BseAI 377 TCCGGA T^CCGGA BsiEI 323 CGGCCG CG[GA][CT]^CG BspDI 115 ATCGAT AT^CGAT BspEI 377 TCCGGA T^CCGGA BsrI 365 ACTGG ACTGG(1/-1) BstBI 215 TTCGAA TT^CGAA BstF5I 340 CATCC GGATG(2/0) CfoI 418 GCGC GCG^C Cfr13I 39 GGTCC G^G[ACGT]CC ClaI 115 ATCGAT AT^CGAT DsaI 320 CCACGG C^C[GA][CT]GG EagI 323 CGGCCG C^GGCCG EclXI 323 CGGCCG C^GGCCG Eco52I 323 CGGCCG C^GGCCG EcoRI 373 GAATTC G^AATTC FokI 340 CATCC GGATG(9/13) FspI 417 TGCGCA TGC^GCA HhaI 418 GCGC GCG^C HinP1I 418 GCGC G^CGC HincII 97 GTTAAC GT[CT]^[GA]AC HindII 97 GTTAAC GT[CT]^[GA]AC HinfI 50 GACTC G^A[ACGT]TC HpaI 97 GTTAAC GTT^AAC HphI 361 GGTGA GGTGA(8/7) MaeII 391 ACGT A^CGT MaeIII 362 GTGAC ^GT[ACGT]AC MfeI 275 CAATTG C^AATTG MluNI 317 TGGCCA TGG^CCA MroI 377 TCCGGA T^CCGGA MscI 317 TGGCCA TGG^CCA MslI 195 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 275 CAATTG C^AATTG NciI 46 CCCGG CC^[GC]GG NruI 446 TCGCGA TCG^CGA NspV 215 TTCGAA TT^CGAA PflMI 30 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 50 GACTC GAGTC(4/5) Sau96I 39 GGTCC G^G[ACGT]CC SfaNI 268 GCATC GCATC(5/9) SfuI 215 TTCGAA TT^CGAA SmlI 409 CTTGAG C^T[CT][GA]AG TseI 141 GCAGC G^C[AT]GC Tsp45I 362 GTGAC ^GT[GC]AC Van91I 30 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 369 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 335 CGCG CG^CG 447 CGCG AluI 57 AGCT AG^CT 91 AGCT BbsI 84 GAAGAC GAAGAC(2/6) 184 GAAGAC BpuAI 84 GAAGAC GAAGAC(2/6) 184 GAAGAC BstUI 335 CGCG CG^CG 447 CGCG Cac8I 8 GCCTGC GC[ACGT]^[ACGT]GC 420 GCAGGC DpnII 114 GATC ^GATC 450 GATC EaeI 317 TGGCCA [CT]^GGCC[GA] 323 CGGCCG HapII 47 CCGG C^CGG 378 CCGG HpaII 47 CCGG C^CGG 378 CCGG MboI 114 GATC ^GATC 450 GATC MboII 84 GAAGA GAAGA(8/7) 184 GAAGA MspI 47 CCGG C^CGG 378 CCGG MvnI 335 CGCG CG^CG 447 CGCG NdeII 114 GATC ^GATC 450 GATC Sau3AI 114 GATC ^GATC 450 GATC TaqI 116 TCGA T^CGA 216 TCGA TthHB8I 116 TCGA T^CGA 216 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI BsaJI BsiYI BslI BsmI BssKI BstNI EcoRII Fnu4HI HaeIII ItaI MnlI MseI MvaI MwoI NlaIII ScrFI Tru9I Tsp509I