Restriction map for
pFN21AB6782
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI TseI Tth111I TthHB8I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 3 GTAC GT^AC
AflII 297 CTTAAG C^TTAAG
Alw44I 59 GTGCAC G^TGCAC
AlwI 352 GGATC GGATC(4/5)
ApaLI 59 GTGCAC G^TGCAC
AseI 142 ATTAAT AT^TAAT
AsnI 142 ATTAAT AT^TAAT
AspHI 59 GTGCAC G[AT]GC[AT]^C
BfaI 235 CTAG C^TAG
BfrI 297 CTTAAG C^TTAAG
BmyI 59 GTGCAC G[AGT]GC[ACT]^C
BsiHKAI 59 GTGCAC G[AT]GC[AT]^C
BsmAI 388 GAGAC GTCTC(1/5)
BsmFI 308 GGGAC GGGAC(10/14)
Bsp1286I 59 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 2 TGTACA T^GTACA
BsrDI 27 CATTGC GCAATG(2/0)
BsrGI 2 TGTACA T^GTACA
BsrI 99 ACTGG ACTGG(1/-1)
BstYI 352 GGATCT [GA]^GATC[CT]
DdeI 34 CTAAG C^T[ACGT]AG
DpnII 353 GATC ^GATC
HaeIII 207 GGCC GG^CC
HinfI 156 GAATC G^A[ACGT]TC
HphI 167 TCACC GGTGA(8/7)
MaeI 235 CTAG C^TAG
MaeII 329 ACGT A^CGT
MaeIII 49 GTCAC ^GT[ACGT]AC
MboI 353 GATC ^GATC
MflI 352 GGATCT [GA]^GATC[CT]
NdeII 353 GATC ^GATC
NspI 194 ACATGC [GA]CATG^[CT]
PshBI 142 ATTAAT AT^TAAT
RsaI 3 GTAC GT^AC
Sau3AI 353 GATC ^GATC
SmlI 297 CTTAAG C^T[CT][GA]AG
SspBI 2 TGTACA T^GTACA
TfiI 156 GAATC G^A[AT]TC
Tsp45I 49 GTCAC ^GT[GC]AC
XbaI 234 TCTAGA T^CTAGA
XhoII 352 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 270 AAATTT [GA]^AATT[CT]
406 GAATTT
AluI 92 AGCT AG^CT
276 AGCT
ApoI 270 AAATTT [GA]^AATT[CT]
406 GAATTT
BpmI 100 CTGGAG CTGGAG(16/14)
256 CTGGAG
BsaJI 304 CCCAGG C^C[ACGT][ACGT]GG
305 CCAGGG
BssKI 170 CCAGG ^CC[ACGT]GG
305 CCAGG
BstF5I 8 GGATG GGATG(2/0)
173 GGATG
BstNI 170 CCAGG CC^[AT]GG
305 CCAGG
EcoRII 170 CCAGG ^CC[AT]GG
305 CCAGG
FokI 8 GGATG GGATG(9/13)
173 GGATG
MnlI 253 CCTC CCTC(7/6)
259 GAGG
MslI 168 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
365 CATTCATGTG
MvaI 170 CCAGG CC^[AT]GG
305 CCAGG
NlaIII 195 CATG CATG^
369 CATG
ScrFI 170 CCAGG CC^[ACGT]GG
305 CCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
MboII MseI SfaNI Tru9I Tsp509I
TspRI