Restriction map for pFN21AB6782
 (Circular) (Six-base) MAPSORT of: pFN21AB6782.seq  Check: 9293  from: 1  to: 5164



pFN21AB6782  5164 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2974     278 
   Size:         53      83     186     936    1438    2468

  Fragments arranged by size: 

                2468    1438     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2507    1937 
   Size:        570    4594
 
Acc65I G'GTAC_C

Cuts at:   2485    2485 
   Size:       5164
 
AclI AA'CG_TT

Cuts at:   1563    3291    3664    1563 
   Size:       1728     373    3063

  Fragments arranged by size: 

                3063    1728     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2216    3227    4611    4879    1632 
   Size:        584    1011    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1011     584     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2302     820 
   Size:        197    1285    3682

  Fragments arranged by size: 

                3682    1285     197
 
AflIII A'CryG_T

Cuts at:   1203    4063    5040    1203 
   Size:       2860     977    1327

  Fragments arranged by size: 

                2860    1327     977
 
AgeI A'CCGG_T

Cuts at:   3995    3995 
   Size:       5164
 
AhdI GACnn_n'nnGTC

Cuts at:   3893    3893 
   Size:       5164
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5164
 
AlwNI CAG_nnn'CTG

Cuts at:   2907    4479    2907 
   Size:       1572    3592
 
ApaLI G'TGCA_C

Cuts at:   2064    3221    4377    2064 
   Size:       1157    1156    2851

  Fragments arranged by size: 

                2851    1157    1156
 
ApoI r'AATT_y

Cuts at:   1493    2275    2411    2473    2699    2851    4960    1493 
   Size:        782     136      62     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     782     226     152     136      62
 
AseI AT'TA_AT

Cuts at:    160    1032    2148    3718     160 
   Size:        872    1116    1570    1606

  Fragments arranged by size: 

                1606    1570    1116     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5164
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2489    1870 
   Size:         73     546    4545

  Fragments arranged by size: 

                4545     546      73
 
BamHI G'GATC_C

Cuts at:   2494    2861    2494 
   Size:        367    4797
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2485    3940     618 
   Size:        325     217     627     698    1455    1842

  Fragments arranged by size: 

                1842    1455     698     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2483    1391 
   Size:       1092    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4193
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3355    3389    1903 
   Size:         34    1418      34    3678

  Fragments arranged by size: 

                3678    1418      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3056    4266    3056 
   Size:       1210    3954
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5164
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3775     136 
   Size:        107     122      71     631     605    2103    1525

  Fragments arranged by size: 

                2103    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5159    5159 
   Size:       5164
 
BlpI GC'TnA_GC

Cuts at:   2581    2581 
   Size:       5164
 
Bme1580I G_kGCm'C

Cuts at:   1790    2068    3225    4381    1790 
   Size:        278    1157    1156    2573

  Fragments arranged by size: 

                2573    1157    1156     278
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5164
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2895    3853     442 
   Size:        416      53      83     399      24    1478     958    1753

  Fragments arranged by size: 

                1753    1478     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5164
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2126    2282    3824    1239 
   Size:        887     156    1542    2579

  Fragments arranged by size: 

                2579    1542     887     156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3289    4154    4452    3289 
   Size:        865     298    4001

  Fragments arranged by size: 

                4001     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3827     882 
   Size:        617    2328    2219

  Fragments arranged by size: 

                2328    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2955     493 
   Size:        711    1751    2702

  Fragments arranged by size: 

                2702    1751     711
 
BsaBI GATnn'nnATC

Cuts at:   5158    5158 
   Size:       5164
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2971    3353    3988     275 
   Size:         53      83     186     936    1438     382     635    1451

  Fragments arranged by size: 

                1451    1438     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3597    3995    4269    4416    4827    3597 
   Size:        398     274     147     411    3934

  Fragments arranged by size: 

                3934     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4939

  Fragments arranged by size: 

                4939     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5164
 
BseYI C'CCAG_C

Cuts at:   4367    4863    4367 
   Size:        496    4668
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4591
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2614    3375    4403    1540 
   Size:        275     186     613     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     613     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2068    3225    3310    4381    2068 
   Size:       1157      85    1071    2851

  Fragments arranged by size: 

                2851    1157    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2675    2768    2675 
   Size:         93    5071
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2068    2483    3225    3310    4381    1391 
   Size:        399     278     415     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     415     399     278      85
 
BspEI T'CCGG_A

Cuts at:   4827    4827 
   Size:       5164
 
BspHI T'CATG_A

Cuts at:   3053    4783    3053 
   Size:       1730    3434
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2519     844 
   Size:       1675    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3051    1394 
   Size:        429    1228    3507

  Fragments arranged by size: 

                3507    1228     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2031    3659    3833      65 
   Size:       1183     783    1628     174    1396

  Fragments arranged by size: 

                1628    1396    1183     783     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3808    3995    1700 
   Size:        110    1998     187    2869

  Fragments arranged by size: 

                2869    1998     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2007      96 
   Size:       1911    3253
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5164
 
BssSI C'ACGA_G

Cuts at:   3224    4236    3224 
   Size:       1012    4152
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5164
 
BstBI TT'CG_AA

Cuts at:   3968    3968 
   Size:       5164
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5164
 
BstYI r'GATC_y

Cuts at:   1972    2357    2494    2861    3246    3263    4715    4801    4813 
   Size:        385     137     367     385      17    1452      86      12

Cuts at:   4813    5159    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     385     367     346     137      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4612
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4425

  Fragments arranged by size: 

                4425     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2680    3474    3494    2680 
   Size:        794      20    4350

  Fragments arranged by size: 

                4350     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5164
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5129
 
DraI TTT'AAA

Cuts at:   2468    2468 
   Size:       5164
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4171     809 
   Size:        507    2855    1802

  Fragments arranged by size: 

                2855    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2611    3500    5129 
   Size:         54    1425      50     275      18     781     889    1629

Cuts at:   5129       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     781     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2611    1537 
   Size:        275     799    4090

  Fragments arranged by size: 

                4090     799     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3094    1353 
   Size:       1741    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3751    4125    4271     387 
   Size:       1064     138    2162     374     146    1280

  Fragments arranged by size: 

                2162    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2126    2216    2282    3227    3824    4611    4879 
   Size:        393     494      90      66     945     597     787     268

Cuts at:   4879    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     945     787     597     494     393     268      90
                  66
 
EcoRI G'AATT_C

Cuts at:   2473    2851    2473 
   Size:        378    4786
 
FspI TGC'GCA

Cuts at:   3670    3670 
   Size:       5164
 
HaeII r_GCGC'y

Cuts at:   1678    4311    1678 
   Size:       2633    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3805    3837    3879    3911    1373 
   Size:         32     135      32    2233      32      42      32    2626

  Fragments arranged by size: 

                2626    2233     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2508    2754     669 
   Size:        988     281     570     246    3079

  Fragments arranged by size: 

                3079     988     570     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5164
 
HpaI GTT'AAC

Cuts at:   2754    2754 
   Size:       5164
 
KpnI G_GTAC'C

Cuts at:   2489    2489 
   Size:       5164
 
MfeI C'AATT_G

Cuts at:   2763    2763 
   Size:       5164
 
MluI A'CGCG_T

Cuts at:   5040    5040 
   Size:       5164
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4278    4462    1305 
   Size:       2973     184    2007

  Fragments arranged by size: 

                2973    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3342

  Fragments arranged by size: 

                3342    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2177    2374    3122    3481    3640     518 
   Size:        648    1011     197     748     359     159    2042

  Fragments arranged by size: 

                2042    1011     748     648     359     197     159
 
MspA1I CmG'CkG

Cuts at:   1606    2580    3257    4405    4650    1606 
   Size:        974     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     974     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4612
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5164
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5164
 
NotI GC'GGCC_GC

Cuts at:   2611    2611 
   Size:       5164
 
NspI r_CATG'y

Cuts at:   1132    1229    2203    2522    4067    1132 
   Size:         97     974     319    1545    2229

  Fragments arranged by size: 

                2229    1545     974     319      97
 
PasI CC'CwG_GG

Cuts at:   1348    2310    1348 
   Size:        962    4202
 
PciI A'CATG_T

Cuts at:   4063    4063 
   Size:       5164
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5164
 
PmeI GTTT'AAAC

Cuts at:   2468    2468 
   Size:       5164
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3233    3265    4746    4778    3233 
   Size:         32    1481      32    3619

  Fragments arranged by size: 

                3619    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5164
 
PsiI TTA'TAA

Cuts at:   2734    2734 
   Size:       5164
 
PstI C_TGCA'G

Cuts at:    830    2516     830 
   Size:       1686    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5164
 
SalI G'TCGA_C

Cuts at:   1936    2506    1936 
   Size:        570    4594
 
SbfI CC_TGCA'GG

Cuts at:   2516    2516 
   Size:       5164
 
ScaI AGT'ACT

Cuts at:   3412    5124    3412 
   Size:       1712    3452
 
SfcI C'TryA_G

Cuts at:    826    1045    2512    3647    4328    4519     826 
   Size:        219    1467    1135     681     191    1471

  Fragments arranged by size: 

                1471    1467    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5164
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5164
 
SmaI CCC'GGG

Cuts at:   1872    2491    1872 
   Size:        619    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2302    3268    4169    4431     820 
   Size:        197     926     359     966     901     262    1553

  Fragments arranged by size: 

                1553     966     926     901     359     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2955     493 
   Size:       2462    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5164
 
SphI G_CATG'C

Cuts at:   1132    2522    1132 
   Size:       1390    3774
 
SspI AAT'ATT

Cuts at:      5      52    3088       5 
   Size:         47    3036    2081

  Fragments arranged by size: 

                3036    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5164
 
StyI C'CwwG_G

Cuts at:    513    1065    2603     513 
   Size:        552    1538    3074

  Fragments arranged by size: 

                3074    1538     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3191    3208    3361    3546    3991     325 
   Size:       1255    1611      17     153     185     445    1498

  Fragments arranged by size: 

                1611    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2007    3410    5122 
   Size:        274      80      33      51    1443      30    1403    1712

Cuts at:   5122      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1403     274     138      80      51      33
                  30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5124    5156    5124 
   Size:         32    5132
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5164
 
XbaI T'CTAG_A

Cuts at:   2239    2500    2239 
   Size:        261    4903
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5164
 
XmaI C'CCGG_G

Cuts at:   1870    2489    1870 
   Size:        619    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3293    3293 
   Size:       5164
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2972     276 
   Size:         53      83     186     936    1438    2468

  Fragments arranged by size: 

                2468    1438     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com