################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MamI MfeI MluI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccI 1035 GTATAC GT^[AC][GT]AC AccIII 1068 TCCGGA T^CCGGA AciI 226 GCGG CCGC(-3/-1) Alw44I 930 GTGCAC G^TGCAC AlwNI 317 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1291 CCTGAGG CC^T[ACGT]AGG Aor51HI 1331 AGCGCT AGC^GCT ApaI 1164 GGGCCC GGGCC^C ApaLI 930 GTGCAC G^TGCAC AspHI 930 GTGCAC G[AT]GC[AT]^C AvaI 961 CCCGAG C^[CT]CG[GA]G BalI 821 TGGCCA TGG^CCA BanII 1164 GGGCCC G[GA]GC[CT]^C BbvCI 263 CCTCAGC CCTCAGC(-5/-2) BcgI 355 CGAAAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 60 CCGGG CC^[GC]GG BlpI 696 GCTCAGC GC^T[ACGT]AGC Bpu1102I 696 GCTCAGC GC^T[ACGT]AGC BsaAI 406 TACGTG [CT]AC^GT[GA] BsaWI 1068 TCCGGA [AT]^CCGG[AT] BseAI 1068 TCCGGA T^CCGGA BsiHKAI 930 GTGCAC G[AT]GC[AT]^C BsmFI 1206 GGGAC GGGAC(10/14) BsmI 1120 GAATGC GAATGC(1/-1) BsoBI 961 CCCGAG C^[CT]CG[GA]G Bsp1407I 140 TGTACA T^GTACA BspEI 1068 TCCGGA T^CCGGA BsrGI 140 TGTACA T^GTACA Bst1107I 1035 GTATAC GTA^TAC BstAPI 268 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 450 AGATCC [GA]^GATC[CT] BstZ17I 1035 GTATAC GTA^TAC Bsu36I 1291 CCTGAGG CC^T[ACGT]AGG CelII 696 GCTCAGC GC^T[ACGT]AGC CfoI 1332 GCGC GCG^C EaeI 821 TGGCCA [CT]^GGCC[GA] EarI 3 GAAGAG CTCTTC(1/4) Eco47III 1331 AGCGCT AGC^GCT Eco81I 1291 CCTGAGG CC^T[ACGT]AGG EcoNI 1080 CCTCAGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 1331 AGCGCT [GA]GCGC^[CT] HhaI 1332 GCGC GCG^C HinP1I 1332 GCGC G^CGC HincII 1324 GTTAAC GT[CT]^[GA]AC HindII 1324 GTTAAC GT[CT]^[GA]AC HpaI 1324 GTTAAC GTT^AAC Ksp632I 3 GAAGAG CTCTTC(1/4) MflI 450 AGATCC [GA]^GATC[CT] MluNI 821 TGGCCA TGG^CCA MroI 1068 TCCGGA T^CCGGA MscI 821 TGGCCA TGG^CCA MspA1I 947 CAGCTG C[AC]G^C[GT]G NciI 60 CCGGG CC^[GC]GG NcoI 914 CCATGG C^CATGG NspI 42 ACATGC [GA]CATG^[CT] PshAI 892 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 947 CAGCTG CAG^CTG SfaNI 1399 GATGC GCATC(5/9) SmlI 1093 CTTGAG C^T[CT][GA]AG SpeI 132 ACTAGT A^CTAGT SspBI 140 TGTACA T^GTACA SspI 485 AATATT AAT^ATT TfiI 1387 GATTC G^A[AT]TC XcmI 338 CCAAAGAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 450 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 451 GATCC GGATC(4/5) 1006 GATCC BbsI 889 GAAGAC GAAGAC(2/6) 1135 GAAGAC BfaI 133 CTAG C^TAG 760 CTAG BmyI 930 GTGCAC G[AGT]GC[ACT]^C 1164 GGGCCC BpmI 610 CTCCAG CTGGAG(16/14) 742 CTGGAG BpuAI 889 GAAGAC GAAGAC(2/6) 1135 GAAGAC BseRI 509 CTCCTC GAGGAG(10/8) 1373 CTCCTC BsgI 333 CTGCAC GTGCAG(16/14) 1220 GTGCAG BsiYI 384 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1080 CCTCAGTAAGG BslI 384 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1080 CCTCAGTAAGG BsmAI 606 GAGAC GTCTC(1/5) 202 GTCTC Bsp1286I 930 GTGCAC G[AGT]GC[ACT]^C 1164 GGGCCC BstF5I 780 CATCC GGATG(2/0) 747 GGATG BstXI 1019 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1304 CCACGGAACTGG DsaI 914 CCATGG C^C[GA][CT]GG 1304 CCACGG EcoT14I 914 CCATGG C^C[AT][AT]GG 1113 CCTTGG FokI 780 CATCC GGATG(9/13) 747 GGATG HapII 60 CCGG C^CGG 1069 CCGG HpaII 60 CCGG C^CGG 1069 CCGG MaeI 133 CTAG C^TAG 760 CTAG MspI 60 CCGG C^CGG 1069 CCGG StyI 914 CCATGG C^C[AT][AT]GG 1113 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AcsI AfaI AluI ApoI AvaII BbvI BsaJI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp45I Tsp509I TspRI