(Linear) (Six-base) MAPSORT of: KIEE1935.seq Check: 5336 from: 1 to: 1422 KIEE1935.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AccI GT'mk_AC Cuts at: 0 1036 1422 Size: 1036 386 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 785 830 1422 Size: 785 45 592 Fragments arranged by size: 785 592 45 AfeI AGC'GCT Cuts at: 0 1333 1422 Size: 1333 89 AlwNI CAG_nnn'CTG Cuts at: 0 322 1422 Size: 322 1100 ApaI G_GGCC'C Cuts at: 0 1168 1422 Size: 1168 254 ApaLI G'TGCA_C Cuts at: 0 930 1422 Size: 930 492 ApoI r'AATT_y Cuts at: 0 91 151 394 876 1422 Size: 91 60 243 482 546 Fragments arranged by size: 546 482 243 91 60 AvaI C'yCGr_G Cuts at: 0 961 1422 Size: 961 461 BanII G_rGCy'C Cuts at: 0 1168 1422 Size: 1168 254 BbsI GAAGACnn'nnnn_ Cuts at: 0 896 1142 1422 Size: 896 246 280 Fragments arranged by size: 896 280 246 BbvCI CC'TCA_GC Cuts at: 0 264 1422 Size: 264 1158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 344 378 1422 Size: 344 34 1044 Fragments arranged by size: 1044 344 34 BlpI GC'TnA_GC Cuts at: 0 697 1422 Size: 697 725 Bme1580I G_kGCm'C Cuts at: 0 934 1168 1422 Size: 934 234 254 Fragments arranged by size: 934 254 234 BmrI ACTGGGnnnn_n' Cuts at: 0 152 1422 Size: 152 1270 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 822 1422 Size: 822 600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 595 763 1422 Size: 595 168 659 Fragments arranged by size: 659 595 168 Bpu10I CC'TnA_GC Cuts at: 0 264 1160 1422 Size: 264 896 262 Fragments arranged by size: 896 264 262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 1422 Size: 1114 308 BsaAI yAC'GTr Cuts at: 0 408 1422 Size: 408 1014 BsaWI w'CCGG_w Cuts at: 0 1068 1422 Size: 1068 354 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 769 799 1330 1360 1422 Size: 769 30 531 30 62 Fragments arranged by size: 769 531 62 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 500 1364 1422 Size: 500 864 58 Fragments arranged by size: 864 500 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 1241 1422 Size: 318 923 181 Fragments arranged by size: 923 318 181 BsiHKAI G_wGCw'C Cuts at: 0 934 1422 Size: 934 488 BsmI GAATG_Cn' Cuts at: 0 1126 1422 Size: 1126 296 Bsp1286I G_dGCh'C Cuts at: 0 934 1168 1422 Size: 934 234 254 Fragments arranged by size: 934 254 234 BspEI T'CCGG_A Cuts at: 0 1068 1422 Size: 1068 354 BsrGI T'GTAC_A Cuts at: 0 140 1422 Size: 140 1282 BstAPI GCAn_nnn'nTGC Cuts at: 0 274 1422 Size: 274 1148 BstXI CCAn_nnnn'nTGG Cuts at: 0 1026 1311 1422 Size: 1026 285 111 Fragments arranged by size: 1026 285 111 BstYI r'GATC_y Cuts at: 0 450 1422 Size: 450 972 BstZ17I GTA'TAC Cuts at: 0 1037 1422 Size: 1037 385 Bsu36I CC'TnA_GG Cuts at: 0 1292 1422 Size: 1292 130 BtgI C'CryG_G Cuts at: 0 914 1304 1422 Size: 914 390 118 Fragments arranged by size: 914 390 118 BtsI GCAGTG_nn' Cuts at: 0 320 1422 Size: 320 1102 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 460 495 1422 Size: 460 35 927 Fragments arranged by size: 927 460 35 EaeI y'GGCC_r Cuts at: 0 821 1422 Size: 821 601 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 595 763 785 830 1422 Size: 595 168 22 45 592 Fragments arranged by size: 595 592 168 45 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 1084 1422 Size: 1084 338 EcoO109I rG'GnC_Cy Cuts at: 0 9 260 345 560 1164 1165 1422 Size: 9 251 85 215 604 1 257 Fragments arranged by size: 604 257 251 215 85 9 1 EcoRI G'AATT_C Cuts at: 0 91 394 876 1422 Size: 91 303 482 546 Fragments arranged by size: 546 482 303 91 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 132 1422 Size: 132 1290 HaeII r_GCGC'y Cuts at: 0 1335 1422 Size: 1335 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 673 705 850 882 1330 1362 Size: 106 32 535 32 145 32 448 32 Cuts at: 1362 1422 Size: 60 Fragments arranged by size: 535 448 145 106 60 32 32 32 32 HincII GTy'rAC Cuts at: 0 1326 1422 Size: 1326 96 HpaI GTT'AAC Cuts at: 0 1326 1422 Size: 1326 96 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1373 1422 Size: 1373 49 MscI TGG'CCA Cuts at: 0 823 1422 Size: 823 599 MspA1I CmG'CkG Cuts at: 0 949 1422 Size: 949 473 NcoI C'CATG_G Cuts at: 0 914 1422 Size: 914 508 NspI r_CATG'y Cuts at: 0 46 1422 Size: 46 1376 PfoI T'CCnGG_A Cuts at: 0 1008 1422 Size: 1008 414 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 344 376 1422 Size: 344 32 1046 Fragments arranged by size: 1046 344 32 PpuMI rG'GwC_Cy Cuts at: 0 9 260 560 1422 Size: 9 251 300 862 Fragments arranged by size: 862 300 251 9 PshAI GACnn'nnGTC Cuts at: 0 896 1422 Size: 896 526 PspOMI G'GGCC_C Cuts at: 0 1164 1422 Size: 1164 258 PvuII CAG'CTG Cuts at: 0 949 1422 Size: 949 473 SmlI C'TyrA_G Cuts at: 0 1093 1422 Size: 1093 329 SpeI A'CTAG_T Cuts at: 0 132 1422 Size: 132 1290 SspI AAT'ATT Cuts at: 0 487 1422 Size: 487 935 StyI C'CwwG_G Cuts at: 0 914 1113 1422 Size: 914 199 309 Fragments arranged by size: 914 309 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 226 329 1317 1422 Size: 226 103 988 105 Fragments arranged by size: 988 226 105 103 TatI w'GTAC_w Cuts at: 0 140 1250 1422 Size: 140 1110 172 Fragments arranged by size: 1110 172 140 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 380 412 1422 Size: 380 32 1010 Fragments arranged by size: 1010 380 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 345 1422 Size: 345 1077 Enzymes that do cut: AccI AcuI AfeI AlwNI ApaI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI EaeI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI MmeI MscI MspA1I NcoI NspI PfoI PpiI PpuMI PshAI PspOMI PvuII SmlI SpeI SspI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI BclI BfrBI BglI BglII BmgBI BmtI BsaI BsaBI BsaHI BseYI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BtgZI ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI