(Circular) (Six-base) MAPSORT of: pFN21AE3891.seq Check: 4918 from: 1 to: 5734 pFN21AE3891 5734 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 3797 5181 5449 1632 Size: 599 1566 1384 268 1917 Fragments arranged by size: 1917 1566 1384 599 268 AfeI AGC'GCT Cuts at: 2459 2459 Size: 5734 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2680 4633 5610 1203 Size: 1477 1953 977 1327 Fragments arranged by size: 1953 1477 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2388 1184 Size: 1204 4530 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2916 2948 2916 Size: 32 5702 AlwNI CAG_nnn'CTG Cuts at: 3477 5049 3477 Size: 1572 4162 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 2561 2603 3043 3269 3421 5530 1493 Size: 1068 42 440 226 152 2109 1697 Fragments arranged by size: 2109 1697 1068 440 226 152 42 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 3059 1870 Size: 73 1116 4545 Fragments arranged by size: 4545 1116 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3055 4510 618 Size: 325 217 627 1268 1455 1842 Fragments arranged by size: 1842 1455 1268 627 325 217 BanII G_rGCy'C Cuts at: 1391 3053 1391 Size: 1662 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 2237 928 Size: 971 302 36 4425 Fragments arranged by size: 4425 971 302 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 3795 4951 1790 Size: 2005 1156 2573 Fragments arranged by size: 2573 2005 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2877 2894 3465 Size: 416 53 83 399 24 1460 17 571 Cuts at: 3465 4423 442 Size: 958 1753 Fragments arranged by size: 1753 1460 958 571 416 399 83 53 24 17 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3009 4394 1239 Size: 1770 1385 2579 Fragments arranged by size: 2579 1770 1385 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2501 4397 882 Size: 617 1002 1896 2219 Fragments arranged by size: 2219 1896 1002 617 BsaAI yAC'GTr Cuts at: 493 1204 2681 3525 493 Size: 711 1477 844 2702 Fragments arranged by size: 2702 1477 844 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2916 2946 1178 Size: 30 165 30 1513 30 3966 Fragments arranged by size: 3966 1513 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2379 1186 Size: 1081 112 4541 Fragments arranged by size: 4541 1081 112 BseYI C'CCAG_C Cuts at: 2171 2424 4937 5433 2171 Size: 253 2513 496 2472 Fragments arranged by size: 2513 2472 496 253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2358 1163 Size: 573 622 4539 Fragments arranged by size: 4539 622 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3053 3795 3880 4951 1391 Size: 399 1263 742 85 1071 2174 Fragments arranged by size: 2174 1263 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3089 844 Size: 2245 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstYI r'GATC_y Cuts at: 1972 3064 3431 3816 3833 5285 5371 5383 5729 Size: 1092 367 385 17 1452 86 12 346 Cuts at: 5729 1972 Size: 1977 Fragments arranged by size: 1977 1452 1092 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4243 Fragments arranged by size: 4243 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2193 2588 3250 4044 4064 2193 Size: 395 662 794 20 3863 Fragments arranged by size: 3863 794 662 395 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3181 4070 5699 Size: 54 1425 50 275 18 1351 889 1629 Cuts at: 5699 8 Size: 43 Fragments arranged by size: 1629 1425 1351 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3664 1353 Size: 2311 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 3009 3797 4394 5181 5449 1239 Size: 393 599 778 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 778 599 597 393 268 EcoRI G'AATT_C Cuts at: 2561 3043 3421 2561 Size: 482 378 4874 Fragments arranged by size: 4874 482 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2512 2581 2512 Size: 69 5665 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2461 2676 4881 1678 Size: 783 215 2205 2531 Fragments arranged by size: 2531 2205 783 215 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 4375 4407 4449 Size: 32 135 32 1109 32 1662 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 1662 1109 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 2790 3333 2790 Size: 543 5191 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4848 5032 1305 Size: 3543 184 2007 Fragments arranged by size: 3543 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3912 Fragments arranged by size: 3912 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 4051 4210 518 Size: 648 2526 359 159 2042 Fragments arranged by size: 2526 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2722 2980 3150 3827 4975 5220 1606 Size: 1116 258 170 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 677 258 245 170 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4243 Fragments arranged by size: 4243 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PmlI CAC'GTG Cuts at: 2681 2681 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2916 2948 3803 3835 5316 5348 2916 Size: 32 855 32 1481 32 3302 Fragments arranged by size: 3302 1481 855 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 2627 3304 2627 Size: 677 5057 PstI C_TGCA'G Cuts at: 830 2188 2341 3086 830 Size: 1358 153 745 3478 Fragments arranged by size: 3478 1358 745 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2980 2980 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 2883 3982 5694 2883 Size: 1099 1712 2923 Fragments arranged by size: 2923 1712 1099 SfcI C'TryA_G Cuts at: 826 1045 2184 2337 2541 2550 2975 3082 4217 Size: 219 1139 153 204 9 425 107 1135 Cuts at: 4217 4898 5089 826 Size: 681 191 1471 Fragments arranged by size: 1471 1139 1135 681 425 219 204 191 153 107 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3838 4739 5001 820 Size: 197 926 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 2107 3658 5 Size: 47 2055 1551 2081 Fragments arranged by size: 2081 2055 1551 47 StuI AGG'CCT Cuts at: 1517 2043 1517 Size: 526 5208 StyI C'CwwG_G Cuts at: 513 1065 2004 2782 3173 513 Size: 552 939 778 391 3074 Fragments arranged by size: 3074 939 778 552 391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3761 3778 3931 4116 4561 325 Size: 1255 2181 17 153 185 445 1498 Fragments arranged by size: 2181 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2881 3980 5692 Size: 274 80 33 51 1443 904 1099 1712 Cuts at: 5692 96 Size: 138 Fragments arranged by size: 1712 1443 1099 904 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2778 2810 5694 5726 2778 Size: 32 2884 32 2786 Fragments arranged by size: 2884 2786 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 2448 2495 3863 2448 Size: 47 1368 4319 Fragments arranged by size: 4319 1368 47 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI