(Circular) (Six-base) MAPSORT of: pFN21AE5579.seq Check: 1537 from: 1 to: 5770 pFN21AE5579 5770 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3018 3113 1937 Size: 1081 95 4594 Fragments arranged by size: 4594 1081 95 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2184 2814 3833 5217 5485 1632 Size: 552 630 1019 1384 268 1917 Fragments arranged by size: 1917 1384 1019 630 552 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 2219 4601 2219 Size: 2382 3388 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaI G_GGCC'C Cuts at: 2701 2701 Size: 5770 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 2378 2404 2452 3046 3079 3305 3457 5566 Size: 885 26 48 594 33 226 152 2109 Cuts at: 5566 1493 Size: 1697 Fragments arranged by size: 2109 1697 885 594 226 152 48 33 26 AseI AT'TA_AT Cuts at: 160 1032 2429 4324 160 Size: 872 1397 1895 1606 Fragments arranged by size: 1895 1606 1397 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 4546 618 Size: 325 217 627 1304 1455 1842 Fragments arranged by size: 1842 1455 1304 627 325 217 BanII G_rGCy'C Cuts at: 1391 2701 3089 1391 Size: 1310 388 4072 Fragments arranged by size: 4072 1310 388 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 928 Size: 971 963 3836 Fragments arranged by size: 3836 971 963 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2557 3662 4872 2557 Size: 1105 1210 3455 Fragments arranged by size: 3455 1210 1105 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2333 2797 2908 5765 2333 Size: 464 111 2857 2338 Fragments arranged by size: 2857 2338 464 111 BlpI GC'TnA_GC Cuts at: 2049 3187 2049 Size: 1138 4632 Bme1580I G_kGCm'C Cuts at: 1790 2701 3831 4987 1790 Size: 911 1130 1156 2573 Fragments arranged by size: 2573 1156 1130 911 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4430 1239 Size: 3191 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3895 4760 5058 3895 Size: 865 298 4607 Fragments arranged by size: 4607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 2739 5764 2739 Size: 3025 2745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2219 2780 4203 4601 4875 5022 5433 2219 Size: 561 1423 398 274 147 411 2556 Fragments arranged by size: 2556 1423 561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5545 Fragments arranged by size: 5545 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2172 2696 1186 Size: 986 524 4260 Fragments arranged by size: 4260 986 524 BseYI C'CCAG_C Cuts at: 4973 5469 4973 Size: 496 5274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2050 3831 3916 4987 2050 Size: 1781 85 1071 2833 Fragments arranged by size: 2833 1781 1071 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2701 3089 3831 3916 4987 1391 Size: 399 260 651 388 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 388 260 85 BspEI T'CCGG_A Cuts at: 2780 5433 2780 Size: 2653 3117 BspHI T'CATG_A Cuts at: 2020 3659 5389 2020 Size: 1639 1730 2401 Fragments arranged by size: 2401 1730 1639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3125 844 Size: 2281 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2254 4265 4439 65 Size: 1183 1006 2011 174 1396 Fragments arranged by size: 2011 1396 1183 1006 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2219 4414 4601 1700 Size: 110 409 2195 187 2869 Fragments arranged by size: 2869 2195 409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2333 2797 2908 3100 3467 3852 3869 5321 Size: 361 464 111 192 367 385 17 1452 Cuts at: 5321 5407 5419 5765 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 464 385 367 361 346 192 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2775 487 Size: 683 56 1549 3482 Fragments arranged by size: 3482 1549 683 56 BtsI GCAGTG_nn' Cuts at: 3286 4080 4100 3286 Size: 794 20 4956 Fragments arranged by size: 4956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735 Size: 54 1425 50 275 18 1387 889 1629 Cuts at: 5735 8 Size: 43 Fragments arranged by size: 1629 1425 1387 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2166 2550 3700 1353 Size: 774 39 384 1150 3423 Fragments arranged by size: 3423 1150 774 384 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2184 2814 3833 4430 5217 5485 1239 Size: 393 552 630 1019 597 787 268 1524 Fragments arranged by size: 1524 1019 787 630 597 552 393 268 EcoO109I rG'GnC_Cy Cuts at: 2697 2698 2697 Size: 1 5769 EcoRI G'AATT_C Cuts at: 2404 3079 3457 2404 Size: 675 378 4717 Fragments arranged by size: 4717 675 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2195 2195 Size: 5770 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373 Size: 32 135 32 2839 32 42 32 2626 Fragments arranged by size: 2839 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3019 3114 3360 669 Size: 988 281 1081 95 246 3079 Fragments arranged by size: 3079 1081 988 281 246 95 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 2109 2964 3369 2109 Size: 855 405 4510 Fragments arranged by size: 4510 855 405 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3948 Fragments arranged by size: 3948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3728 4087 4246 518 Size: 648 2562 359 159 2042 Fragments arranged by size: 2562 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3014 3186 3863 5011 5256 1606 Size: 1408 172 677 1148 245 2120 Fragments arranged by size: 2120 1408 1148 677 245 172 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2445 2770 3128 4673 1132 Size: 97 1216 325 358 1545 2229 Fragments arranged by size: 2229 1545 1216 358 325 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 2352 3340 2352 Size: 988 4782 PspOMI G'GGCC_C Cuts at: 2697 2697 Size: 5770 PstI C_TGCA'G Cuts at: 830 2541 3122 830 Size: 1711 581 3478 Fragments arranged by size: 3478 1711 581 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3017 3112 1936 Size: 1081 95 4594 Fragments arranged by size: 4594 1081 95 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 2752 4018 5730 2752 Size: 1266 1712 2792 Fragments arranged by size: 2792 1712 1266 SfcI C'TryA_G Cuts at: 826 1045 2537 3118 4253 4934 5125 826 Size: 219 1492 581 1135 681 191 1471 Fragments arranged by size: 1492 1471 1135 681 581 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3874 4775 5037 820 Size: 197 926 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 2770 3128 1132 Size: 1638 358 3774 Fragments arranged by size: 3774 1638 358 SspI AAT'ATT Cuts at: 5 52 2084 3694 5 Size: 47 2032 1610 2081 Fragments arranged by size: 2081 2032 1610 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 3209 513 Size: 552 939 1205 3074 Fragments arranged by size: 3074 1205 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3797 3814 3967 4152 4597 325 Size: 1255 2217 17 153 185 445 1498 Fragments arranged by size: 2217 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2750 3004 4016 Size: 274 80 33 51 1443 773 254 1012 Cuts at: 4016 5728 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1012 773 274 254 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI