################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BpmI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KspI MluI MluNI MroI MscI MspI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1648 GGTACC G^GTACC AccII 14 CGCG CG^CG AhdI 1942 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 403 CCTGAGG CC^T[ACGT]AGG Asp718I 1648 GGTACC G^GTACC AspEI 1942 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 1648 GGTACC G^G[CT][GA]CC BanII 2243 GAGCTC G[GA]GC[CT]^C BglI 1024 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1636 AGATCT A^GATCT BlpI 994 GCTCAGC GC^T[ACGT]AGC Bpu1102I 994 GCTCAGC GC^T[ACGT]AGC BsgI 635 GTGCAG GTGCAG(16/14) BsmAI 1078 GAGAC GTCTC(1/5) BspDI 224 ATCGAT AT^CGAT BspHI 54 TCATGA T^CATGA BspMI 1818 ACCTGC ACCTGC(4/8) BstAPI 484 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 14 CGCG CG^CG BstXI 319 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 403 CCTGAGG CC^T[ACGT]AGG CelII 994 GCTCAGC GC^T[ACGT]AGC ClaI 224 ATCGAT AT^CGAT DraII 261 GGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 1022 TGGCCG [CT]^GGCC[GA] Eam1105I 1942 GACATGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 403 CCTGAGG CC^T[ACGT]AGG EcoNI 1800 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 261 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 2204 GATATC GAT^ATC HaeII 1422 GGCGCT [GA]GCGC^[CT] HincII 1923 GTTGAC GT[CT]^[GA]AC HindII 1923 GTTGAC GT[CT]^[GA]AC KpnI 1648 GGTACC GGTAC^C MfeI 720 CAATTG C^AATTG MspA1I 830 CAGCTG C[AC]G^C[GT]G MunI 720 CAATTG C^AATTG MvnI 14 CGCG CG^CG NcoI 1211 CCATGG C^CATGG NdeI 649 CATATG CA^TATG PpuMI 261 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 830 CAGCTG CAG^CTG RcaI 54 TCATGA T^CATGA SacI 2243 GAGCTC GAGCT^C SfcI 1106 CTGTAG C^T[GA][CT]AG SmlI 811 CTTGAG C^T[CT][GA]AG TaqI 225 TCGA T^CGA TthHB8I 225 TCGA T^CGA XbaI 1083 TCTAGA T^CTAGA XcmI 1010 CCACAAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 1850 CCGC CCGC(-3/-1) 465 GCGG AseI 1182 ATTAAT AT^TAAT 1977 ATTAAT AsnI 1182 ATTAAT AT^TAAT 1977 ATTAAT AspHI 946 GAGCAC G[AT]GC[AT]^C 2243 GAGCTC AvaII 262 GGTCC G^G[AT]CC 1711 GGACC BmyI 946 GAGCAC G[AGT]GC[ACT]^C 2243 GAGCTC BseRI 1565 CTCCTC GAGGAG(10/8) 433 GAGGAG BsiHKAI 946 GAGCAC G[AT]GC[AT]^C 2243 GAGCTC BsmFI 119 GGGAC GGGAC(10/14) 1728 GTCCC BsmI 1216 GAATGC GAATGC(1/-1) 1680 GAATGC Bsp1286I 946 GAGCAC G[AGT]GC[ACT]^C 2243 GAGCTC DsaI 1025 CCGTGG C^C[GA][CT]GG 1211 CCATGG EcoT14I 265 CCTTGG C^C[AT][AT]GG 1211 CCATGG HaeIII 317 GGCC GG^CC 1023 GGCC HphI 709 TCACC GGTGA(8/7) 1006 TCACC MaeII 1121 ACGT A^CGT 1229 ACGT MslI 55 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 949 CACACTAGTG PleI 174 GACTC GAGTC(4/5) 1080 GACTC PshBI 1182 ATTAAT AT^TAAT 1977 ATTAAT ScaI 2212 AGTACT AGT^ACT 2233 AGTACT SpeI 952 ACTAGT A^CTAGT 1878 ACTAGT StyI 265 CCTTGG C^C[AT][AT]GG 1211 CCATGG Tsp45I 708 GTCAC ^GT[GC]AC 1118 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AcsI AfaI AflIII AluI AlwI ApoI Asp700I BbsI BbvI BfaI BpuAI BsaBI BsaJI BsiYI BslI BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRI EcoRII Fnu4HI FokI HhaI HinP1I HinfI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI