(Circular) (Six-base) MAPSORT of: pFN21AE1276.seq Check: 6286 from: 1 to: 6961 pFN21AE1276 6961 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4771 278 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4304 1937 Size: 2367 4594 Acc65I G'GTAC_C Cuts at: 3653 4282 3653 Size: 629 6332 AclI AA'CG_TT Cuts at: 1563 5088 5461 1563 Size: 3525 373 3063 Fragments arranged by size: 3525 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2111 2739 3954 4115 5024 6408 6676 Size: 398 81 628 1215 161 909 1384 268 Cuts at: 6676 1632 Size: 1917 Fragments arranged by size: 1917 1384 1215 909 628 398 268 161 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6764 AflIII A'CryG_T Cuts at: 1203 2737 2922 3485 5860 6837 1203 Size: 1534 185 563 2375 977 1327 Fragments arranged by size: 2375 1534 1327 977 563 185 AgeI A'CCGG_T Cuts at: 5792 5792 Size: 6961 AhdI GACnn_n'nnGTC Cuts at: 3952 5690 3952 Size: 1738 5223 AleI CACnn'nnGTG Cuts at: 1166 2958 1166 Size: 1792 5169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6961 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3658 3690 3658 Size: 32 6929 AlwNI CAG_nnn'CTG Cuts at: 4704 6276 4704 Size: 1572 5389 ApaLI G'TGCA_C Cuts at: 5018 6174 5018 Size: 1156 5805 ApoI r'AATT_y Cuts at: 1493 2545 2862 3007 3074 3195 4048 4270 4496 Size: 1052 317 145 67 121 853 222 226 Cuts at: 4496 4648 6757 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1052 853 317 226 222 152 145 121 67 AseI AT'TA_AT Cuts at: 160 1032 3188 3983 5515 160 Size: 872 2156 795 1532 1606 Fragments arranged by size: 2156 1606 1532 872 795 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 AvaI C'yCGr_G Cuts at: 1870 1943 4286 1870 Size: 73 2343 4545 Fragments arranged by size: 4545 2343 73 BamHI G'GATC_C Cuts at: 4291 4658 4291 Size: 367 6594 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3653 4282 5737 618 Size: 325 217 627 1866 629 1455 1842 Fragments arranged by size: 1866 1842 1455 629 627 325 217 BanII G_rGCy'C Cuts at: 1391 4252 4280 1391 Size: 2861 28 4072 Fragments arranged by size: 4072 2861 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3942 4175 4182 928 Size: 971 2043 233 7 3707 Fragments arranged by size: 3707 2043 971 233 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5152 5186 1903 Size: 34 3215 34 3678 Fragments arranged by size: 3678 3215 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4853 6063 4853 Size: 1210 5751 BclI T'GATC_A Cuts at: 1548 1548 Size: 6961 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3035 5572 136 Size: 107 122 71 631 605 1363 2537 1525 Fragments arranged by size: 2537 1525 1363 631 605 122 107 71 BglII A'GATC_T Cuts at: 3641 6956 3641 Size: 3315 3646 BlpI GC'TnA_GC Cuts at: 3000 4378 3000 Size: 1378 5583 Bme1580I G_kGCm'C Cuts at: 1790 5022 6178 1790 Size: 3232 1156 2573 Fragments arranged by size: 3232 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6961 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3173 3659 4692 Size: 416 53 83 399 24 1756 486 1033 Cuts at: 4692 5650 442 Size: 958 1753 Fragments arranged by size: 1756 1753 1033 958 486 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6961 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5621 1239 Size: 4382 2579 Bpu10I CC'TnA_GC Cuts at: 3699 3699 Size: 6961 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2837 5086 5951 6249 2837 Size: 2249 865 298 3549 Fragments arranged by size: 3549 2249 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5624 882 Size: 617 4125 2219 Fragments arranged by size: 4125 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4752 493 Size: 711 3548 2702 Fragments arranged by size: 3548 2702 711 BsaBI GATnn'nnATC Cuts at: 2614 2784 2982 6955 2614 Size: 170 198 3973 2620 Fragments arranged by size: 3973 2620 198 170 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4768 5150 5785 275 Size: 53 83 186 936 3235 382 635 1451 Fragments arranged by size: 3235 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5394 5792 6066 6213 6624 5394 Size: 398 274 147 411 5731 Fragments arranged by size: 5731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3658 3688 1178 Size: 30 165 30 2255 30 4451 Fragments arranged by size: 4451 2255 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 3561 1186 Size: 1267 1108 4586 Fragments arranged by size: 4586 1267 1108 BseYI C'CCAG_C Cuts at: 3735 6164 6660 3735 Size: 2429 496 4036 Fragments arranged by size: 4036 2429 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2661 1163 Size: 573 925 5463 Fragments arranged by size: 5463 925 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4411 5172 6200 1540 Size: 275 186 2410 761 1028 2301 Fragments arranged by size: 2410 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2955 4252 5022 5107 6178 2955 Size: 1297 770 85 1071 3738 Fragments arranged by size: 3738 1297 1071 770 85 BsmI GAATG_Cn' Cuts at: 3227 3691 4472 4565 3227 Size: 464 781 93 5623 Fragments arranged by size: 5623 781 464 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2955 4252 4280 5022 5107 6178 1391 Size: 399 1165 1297 28 742 85 1071 2174 Fragments arranged by size: 2174 1297 1165 1071 742 399 85 28 BspEI T'CCGG_A Cuts at: 6624 6624 Size: 6961 BspHI T'CATG_A Cuts at: 2059 4850 6580 2059 Size: 2791 1730 2440 Fragments arranged by size: 2791 2440 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3832 4316 844 Size: 2988 484 3489 Fragments arranged by size: 3489 2988 484 BsrBI CCG'CTC Cuts at: 1394 1823 4848 1394 Size: 429 3025 3507 Fragments arranged by size: 3507 3025 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5456 5630 65 Size: 1183 4208 174 1396 Fragments arranged by size: 4208 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5605 5792 1700 Size: 110 3795 187 2869 Fragments arranged by size: 3795 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6961 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6961 BssSI C'ACGA_G Cuts at: 5021 6033 5021 Size: 1012 5949 BstAPI GCAn_nnn'nTGC Cuts at: 794 2495 794 Size: 1701 5260 BstBI TT'CG_AA Cuts at: 5765 5765 Size: 6961 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6961 BstXI CCAn_nnnn'nTGG Cuts at: 2331 2331 Size: 6961 BstYI r'GATC_y Cuts at: 1972 2691 2785 3641 4291 4658 5043 5060 6512 Size: 719 94 856 650 367 385 17 1452 Cuts at: 6512 6598 6610 6956 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 856 719 650 385 367 346 94 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2409 2409 Size: 6961 BtgI C'CryG_G Cuts at: 513 1065 2004 3030 3216 513 Size: 552 939 1026 186 4258 Fragments arranged by size: 4258 1026 939 552 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6222 Fragments arranged by size: 6222 683 56 BtsI GCAGTG_nn' Cuts at: 2029 2381 4477 5271 5291 2029 Size: 352 2096 794 20 3699 Fragments arranged by size: 3699 2096 794 352 20 ClaI AT'CG_AT Cuts at: 1554 2230 1554 Size: 676 6285 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6926 DraI TTT'AAA Cuts at: 2247 2355 3777 4265 2247 Size: 108 1422 488 4943 Fragments arranged by size: 4943 1422 488 108 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5968 809 Size: 507 4652 1802 Fragments arranged by size: 4652 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3027 4408 5297 Size: 54 1425 50 275 18 1197 1381 889 Cuts at: 5297 6926 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1381 1197 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4408 1537 Size: 275 2596 4090 Fragments arranged by size: 4090 2596 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2430 3347 3579 4891 1353 Size: 653 424 917 232 1312 3423 Fragments arranged by size: 3423 1312 917 653 424 232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5548 5922 6068 387 Size: 1064 138 3959 374 146 1280 Fragments arranged by size: 3959 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4250 4250 Size: 6961 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2111 2739 3954 4115 5024 5621 Size: 393 398 81 628 1215 161 909 597 Cuts at: 5621 6408 6676 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1215 909 787 628 597 398 393 268 161 81 EcoNI CCTnn'n_nnAGG Cuts at: 3809 3809 Size: 6961 EcoO109I rG'GnC_Cy Cuts at: 2267 2267 Size: 6961 EcoRI G'AATT_C Cuts at: 2545 2862 3007 3195 4270 4648 2545 Size: 317 145 188 1075 378 4858 Fragments arranged by size: 4858 1075 378 317 188 145 EcoRV GAT'ATC Cuts at: 4211 4211 Size: 6961 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2352 4199 2352 Size: 1847 5114 FspI TGC'GCA Cuts at: 5467 5467 Size: 6961 HaeII r_GCGC'y Cuts at: 1678 3431 6108 1678 Size: 1753 2677 2531 Fragments arranged by size: 2677 2531 1753 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 2909 2941 3938 Size: 32 135 32 679 32 626 32 997 Cuts at: 3938 3970 5602 5634 5676 5708 1373 Size: 32 1632 32 42 32 2626 Fragments arranged by size: 2626 1632 997 679 626 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3930 4305 4551 669 Size: 988 281 1992 375 246 3079 Fragments arranged by size: 3079 1992 988 375 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6961 HpaI GTT'AAC Cuts at: 4551 4551 Size: 6961 KpnI G_GTAC'C Cuts at: 3657 4286 3657 Size: 629 6332 MfeI C'AATT_G Cuts at: 2725 4560 2725 Size: 1835 5126 MluI A'CGCG_T Cuts at: 6837 6837 Size: 6961 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6075 6259 1305 Size: 4770 184 2007 Fragments arranged by size: 4770 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5139 Fragments arranged by size: 5139 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2958 4919 5278 5437 518 Size: 648 898 894 1961 359 159 2042 Fragments arranged by size: 2042 1961 898 894 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2837 4377 5054 6202 6447 1606 Size: 1231 1540 677 1148 245 2120 Fragments arranged by size: 2120 1540 1231 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3216 513 Size: 552 939 1212 4258 Fragments arranged by size: 4258 1212 939 552 NdeI CA'TA_TG Cuts at: 387 2655 387 Size: 2268 4693 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6961 NotI GC'GGCC_GC Cuts at: 4408 4408 Size: 6961 NspI r_CATG'y Cuts at: 1132 1229 2741 2926 3489 3952 4319 5864 1132 Size: 97 1512 185 563 463 367 1545 2229 Fragments arranged by size: 2229 1545 1512 563 463 367 185 97 PasI CC'CwG_GG Cuts at: 1348 2696 1348 Size: 1348 5613 PciI A'CATG_T Cuts at: 2737 2922 3485 5860 2737 Size: 185 563 2375 3838 Fragments arranged by size: 3838 2375 563 185 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6961 PfoI T'CCnGG_A Cuts at: 2426 2509 2426 Size: 83 6878 PmeI GTTT'AAAC Cuts at: 4265 4265 Size: 6961 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3658 3690 5030 5062 6543 6575 3658 Size: 32 1340 32 1481 32 4044 Fragments arranged by size: 4044 1481 1340 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2267 2267 Size: 6961 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6961 PsiI TTA'TAA Cuts at: 4531 4531 Size: 6961 PstI C_TGCA'G Cuts at: 830 4313 830 Size: 3483 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6961 PvuII CAG'CTG Cuts at: 2837 2837 Size: 6961 SacI G_AGCT'C Cuts at: 4252 4252 Size: 6961 SalI G'TCGA_C Cuts at: 1936 4303 1936 Size: 2367 4594 SbfI CC_TGCA'GG Cuts at: 4313 4313 Size: 6961 ScaI AGT'ACT Cuts at: 4219 4240 5209 6921 4219 Size: 21 969 1712 4259 Fragments arranged by size: 4259 1712 969 21 SfcI C'TryA_G Cuts at: 826 1045 3111 4309 5444 6125 6316 826 Size: 219 2066 1198 1135 681 191 1471 Fragments arranged by size: 2066 1471 1198 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6961 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6961 SmaI CCC'GGG Cuts at: 1872 4288 1872 Size: 2416 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2816 5065 5966 6228 820 Size: 197 926 873 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 4752 493 Size: 4259 2702 SpeI A'CTAG_T Cuts at: 152 2957 3883 152 Size: 2805 926 3230 Fragments arranged by size: 3230 2805 926 SphI G_CATG'C Cuts at: 1132 4319 1132 Size: 3187 3774 SspI AAT'ATT Cuts at: 5 52 4885 5 Size: 47 4833 2081 Fragments arranged by size: 4833 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6961 StyI C'CwwG_G Cuts at: 513 1065 2004 2270 3216 4400 513 Size: 552 939 266 946 1184 3074 Fragments arranged by size: 3074 1184 946 939 552 266 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3028 4988 5005 5158 5343 5788 325 Size: 1255 1448 1960 17 153 185 445 1498 Fragments arranged by size: 1960 1498 1448 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3244 4217 4238 Size: 274 80 33 51 1443 1267 973 21 Cuts at: 4238 5207 6919 96 Size: 969 1712 138 Fragments arranged by size: 1712 1443 1267 973 969 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3324 3356 6921 6953 3324 Size: 32 3565 32 3332 Fragments arranged by size: 3565 3332 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6961 XbaI T'CTAG_A Cuts at: 3088 4297 3088 Size: 1209 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 3022 Size: 6961 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6961 XmaI C'CCGG_G Cuts at: 1870 4286 1870 Size: 2416 4545 XmnI GAAnn'nnTTC Cuts at: 2307 2739 2884 3291 3762 5090 2307 Size: 432 145 407 471 1328 4178 Fragments arranged by size: 4178 1328 471 432 407 145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4769 276 Size: 53 83 186 936 3235 2468 Fragments arranged by size: 3235 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI