################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HincII HindII HindIII HinfI HpaI KasI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 508 GACGTC GACGT^C Acc65I 462 GGTACC G^GTACC AcyI 508 GACGTC G[GA]^CG[CT]C Asp700I 699 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 462 GGTACC G^GTACC AspHI 482 GAGCTC G[AT]GC[AT]^C AvaI 479 CCCGAG C^[CT]CG[GA]G BalI 365 TGGCCA TGG^CCA BamHI 674 GGATCC G^GATCC BanI 462 GGTACC G^G[CT][GA]CC BanII 482 GAGCTC G[GA]GC[CT]^C BbsI 736 GTCTTC GAAGAC(2/6) BbvCI 615 CCTCAGC CCTCAGC(-5/-2) BcnI 405 CCGGG CC^[GC]GG BfaI 454 CTAG C^TAG BglII 203 AGATCT A^GATCT BpmI 199 CTGGAG CTGGAG(16/14) BpuAI 736 GTCTTC GAAGAC(2/6) BsaHI 508 GACGTC G[GA]^CG[CT]C BsaI 719 GGTCTC GGTCTC(1/5) BseRI 45 CTCCTC GAGGAG(10/8) BsiHKAI 482 GAGCTC G[AT]GC[AT]^C BsmAI 720 GTCTC GTCTC(1/5) BsoBI 479 CCCGAG C^[CT]CG[GA]G BstXI 420 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 384 GCGC GCG^C DraII 65 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 790 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 244 GACCTGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 365 TGGCCA [CT]^GGCC[GA] EcoNI 924 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 65 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 580 GAATTC G^AATTC FauI 716 GCGGG CCCGC(4/6) HapII 405 CCGG C^CGG HhaI 384 GCGC GCG^C Hin1I 508 GACGTC G[GA]^CG[CT]C HinP1I 384 GCGC G^CGC HpaII 405 CCGG C^CGG KpnI 462 GGTACC GGTAC^C MaeI 454 CTAG C^TAG MaeII 509 ACGT A^CGT MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA MseI 884 TTAA T^TAA MspI 405 CCGG C^CGG NciI 405 CCGGG CC^[GC]GG PshAI 606 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 482 GAGCTC GAGCT^C Tru9I 884 TTAA T^TAA XmnI 699 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 672 GCGG CCGC(-3/-1) 716 GCGG AcsI 25 GAATTT [GA]^AATT[CT] 580 GAATTC AlwI 675 GATCC GGATC(4/5) 674 GGATC AlwNI 687 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 910 CAGAGGCTG ApoI 25 GAATTT [GA]^AATT[CT] 580 GAATTC BmyI 226 GTGCCC G[AGT]GC[ACT]^C 482 GAGCTC BsmFI 587 GGGAC GGGAC(10/14) 253 GTCCC Bsp1286I 226 GTGCCC G[AGT]GC[ACT]^C 482 GAGCTC BstEII 210 GGTAACC G^GT[ACGT]ACC 630 GGTGACC BstF5I 333 CATCC GGATG(2/0) 233 GGATG BstPI 210 GGTAACC G^GT[ACGT]ACC 630 GGTGACC BstYI 203 AGATCT [GA]^GATC[CT] 674 GGATCC DsaI 458 CCATGG C^C[GA][CT]GG 501 CCATGG EcoO65I 210 GGTAACC G^GT[ACGT]ACC 630 GGTGACC FokI 333 CATCC GGATG(9/13) 233 GGATG MflI 203 AGATCT [GA]^GATC[CT] 674 GGATCC MslI 129 CATAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 393 CACTCTGATG MspA1I 421 CAGCTG C[AC]G^C[GT]G 690 CAGCTG NcoI 458 CCATGG C^CATGG 501 CCATGG PvuII 421 CAGCTG CAG^CTG 690 CAGCTG SfaNI 234 GATGC GCATC(5/9) 906 GATGC StuI 775 AGGCCT AGG^CCT 921 AGGCCT XhoII 203 AGATCT [GA]^GATC[CT] 674 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AfaI AluI AvaII BbvI BsaJI BsiYI BslI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I Tsp509I TspRI