(Circular) (Six-base) MAPSORT of: pFN21AE5988.seq Check: 9751 from: 1 to: 5644 pFN21AE5988 5644 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2517 3454 278 Size: 53 83 186 936 981 937 2468 Fragments arranged by size: 2468 981 937 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2467 2965 2467 Size: 498 5146 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3707 5091 5359 1632 Size: 1185 890 1384 268 1917 Fragments arranged by size: 1917 1384 1185 890 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 2697 2920 3387 4959 2697 Size: 223 467 1572 3382 Fragments arranged by size: 3382 1572 467 223 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2030 2585 2953 3179 3331 5440 1493 Size: 537 555 368 226 152 2109 1697 Fragments arranged by size: 2109 1697 555 537 368 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2484 2969 1870 Size: 73 541 485 4545 Fragments arranged by size: 4545 541 485 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2743 2776 2743 Size: 33 5611 BamHI G'GATC_C Cuts at: 2679 2974 3341 2679 Size: 295 367 4982 Fragments arranged by size: 4982 367 295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2467 2965 4420 618 Size: 325 217 627 680 498 1455 1842 Fragments arranged by size: 1842 1455 680 627 498 325 217 BanII G_rGCy'C Cuts at: 1391 2491 2963 1391 Size: 1100 472 4072 Fragments arranged by size: 4072 1100 472 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2734 928 Size: 971 835 3838 Fragments arranged by size: 3838 971 835 BbvCI CC'TCA_GC Cuts at: 2621 2621 Size: 5644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2208 5639 2208 Size: 3431 2213 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2235 3705 4861 1790 Size: 445 1470 1156 2573 Fragments arranged by size: 2573 1470 1156 445 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 4304 1239 Size: 986 2079 2579 Fragments arranged by size: 2579 2079 986 Bpu10I CC'TnA_GC Cuts at: 2621 2621 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2730 4307 882 Size: 617 1231 1577 2219 Fragments arranged by size: 2219 1577 1231 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2514 3451 3833 4468 Size: 53 83 186 936 981 937 382 635 Cuts at: 4468 275 Size: 1451 Fragments arranged by size: 1451 981 937 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 1186 Size: 855 4789 BseYI C'CCAG_C Cuts at: 2432 4847 5343 2432 Size: 2415 496 2733 Fragments arranged by size: 2733 2415 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2491 3705 3790 4861 2491 Size: 1214 85 1071 3274 Fragments arranged by size: 3274 1214 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2491 2963 3705 3790 4861 1391 Size: 399 445 256 472 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 445 399 256 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 2215 2635 1184 Size: 1031 420 4193 Fragments arranged by size: 4193 1031 420 BstXI CCAn_nnnn'nTGG Cuts at: 2432 2432 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2208 2679 2974 3341 3726 3743 5195 5281 Size: 236 471 295 367 385 17 1452 86 Cuts at: 5281 5293 5639 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 471 385 367 346 295 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2463 2506 513 Size: 552 939 459 43 3651 Fragments arranged by size: 3651 939 552 459 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2235 2384 3160 3954 3974 2235 Size: 149 776 794 20 3905 Fragments arranged by size: 3905 794 776 149 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DraIII CAC_nnn'GTG Cuts at: 2800 2800 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2255 4651 809 Size: 507 939 2396 1802 Fragments arranged by size: 2396 1802 939 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 3091 3980 Size: 54 1425 50 275 18 540 721 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 721 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2489 2489 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2817 3707 4304 5091 5359 1239 Size: 393 593 592 890 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 593 592 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2933 2933 Size: 5644 EcoO109I rG'GnC_Cy Cuts at: 2071 2071 Size: 5644 EcoRI G'AATT_C Cuts at: 2585 2953 3331 2585 Size: 368 378 4898 Fragments arranged by size: 4898 378 368 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2471 2969 2471 Size: 498 5146 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 4758 4942 1305 Size: 1257 2196 184 2007 Fragments arranged by size: 2196 2007 1257 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 10 Size: 54 1425 343 540 3282 Fragments arranged by size: 3282 1425 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2402 3602 3961 4120 518 Size: 648 972 264 1200 359 159 2042 Fragments arranged by size: 2042 1200 972 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2428 2697 3060 3737 4885 5130 1606 Size: 822 269 363 677 1148 245 2120 Fragments arranged by size: 2120 1148 822 677 363 269 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2463 2506 513 Size: 552 939 459 43 3651 Fragments arranged by size: 3651 939 552 459 43 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 2317 1348 Size: 969 4675 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2615 1613 Size: 1002 4642 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2070 2230 2556 2996 830 Size: 1240 160 326 440 3478 Fragments arranged by size: 3478 1240 440 326 160 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2428 2697 2428 Size: 269 5375 SacI G_AGCT'C Cuts at: 2491 2491 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2066 2226 2552 2752 2900 2992 4127 Size: 219 1021 160 326 200 148 92 1135 Cuts at: 4127 4808 4999 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1021 681 326 219 200 191 160 148 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 2782 2928 1517 Size: 1265 146 4233 Fragments arranged by size: 4233 1265 146 StyI C'CwwG_G Cuts at: 513 1065 2004 2463 2506 2823 3083 513 Size: 552 939 459 43 317 260 3074 Fragments arranged by size: 3074 939 552 459 317 260 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 2185 2397 3671 3688 3841 4026 Size: 1255 591 14 212 1274 17 153 185 Cuts at: 4026 4471 325 Size: 445 1498 Fragments arranged by size: 1498 1274 1255 591 445 212 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3890 5602 Size: 274 80 33 51 1443 198 1715 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1715 1712 1443 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2708 3773 2708 Size: 1065 4579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2515 3452 276 Size: 53 83 186 936 981 937 2468 Fragments arranged by size: 2468 981 937 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI