(Circular) (Six-base) MAPSORT of: pFN21AE1261.seq Check: 5029 from: 1 to: 6517 pFN21AE1261 6517 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4327 278 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3860 1937 Size: 1923 4594 Acc65I G'GTAC_C Cuts at: 3210 3838 3210 Size: 628 5889 AclI AA'CG_TT Cuts at: 1563 4644 5017 1563 Size: 3081 373 3063 Fragments arranged by size: 3081 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2398 4580 5964 6232 1632 Size: 766 2182 1384 268 1917 Fragments arranged by size: 2182 1917 1384 766 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 5416 6393 1203 Size: 4213 977 1327 Fragments arranged by size: 4213 1327 977 AgeI A'CCGG_T Cuts at: 5348 5348 Size: 6517 AhdI GACnn_n'nnGTC Cuts at: 2257 5246 2257 Size: 2989 3528 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6517 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6517 AlwNI CAG_nnn'CTG Cuts at: 4260 5832 4260 Size: 1572 4945 ApaLI G'TGCA_C Cuts at: 4574 5730 4574 Size: 1156 5361 ApoI r'AATT_y Cuts at: 1493 2283 3032 3826 4052 4204 6313 1493 Size: 790 749 794 226 152 2109 1697 Fragments arranged by size: 2109 1697 794 790 749 226 152 AseI AT'TA_AT Cuts at: 160 1032 5071 160 Size: 872 4039 1606 Fragments arranged by size: 4039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 3842 1870 Size: 73 1899 4545 Fragments arranged by size: 4545 1899 73 AvrII C'CTAG_G Cuts at: 2176 3091 2176 Size: 915 5602 BamHI G'GATC_C Cuts at: 3847 4214 3847 Size: 367 6150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3210 3241 3838 5293 618 Size: 325 217 627 1423 31 597 1455 1842 Fragments arranged by size: 1842 1455 1423 627 597 325 217 31 BanII G_rGCy'C Cuts at: 1391 2396 2808 3836 1391 Size: 1005 412 1028 4072 Fragments arranged by size: 4072 1028 1005 412 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 928 Size: 971 354 5192 Fragments arranged by size: 5192 971 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2332 2366 4708 4742 1903 Size: 34 50 34 311 34 2342 34 3678 Fragments arranged by size: 3678 2342 311 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2365 2395 3371 3509 4409 5619 2365 Size: 30 976 138 900 1210 3263 Fragments arranged by size: 3263 1210 976 900 138 30 BclI T'GATC_A Cuts at: 1548 1548 Size: 6517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2840 3494 5128 Size: 107 122 71 631 605 1168 654 1634 Cuts at: 5128 136 Size: 1525 Fragments arranged by size: 1634 1525 1168 654 631 605 122 107 71 BglII A'GATC_T Cuts at: 6512 6512 Size: 6517 BlpI GC'TnA_GC Cuts at: 3934 3934 Size: 6517 Bme1580I G_kGCm'C Cuts at: 1790 2671 4578 5734 1790 Size: 881 1907 1156 2573 Fragments arranged by size: 2573 1907 1156 881 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2480 3065 3329 Size: 416 53 83 399 24 1063 585 264 Cuts at: 3329 3521 3791 4248 5206 442 Size: 192 270 457 958 1753 Fragments arranged by size: 1753 1063 958 585 457 416 399 270 264 192 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5177 1239 Size: 3938 2579 Bpu10I CC'TnA_GC Cuts at: 2802 2802 Size: 6517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4642 5507 5805 4642 Size: 865 298 5354 Fragments arranged by size: 5354 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2901 5180 882 Size: 617 1402 2279 2219 Fragments arranged by size: 2279 2219 1402 617 BsaAI yAC'GTr Cuts at: 493 1204 2109 4308 493 Size: 711 905 2199 2702 Fragments arranged by size: 2702 2199 905 711 BsaBI GATnn'nnATC Cuts at: 6511 6511 Size: 6517 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4324 4706 5341 275 Size: 53 83 186 936 2791 382 635 1451 Fragments arranged by size: 2791 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4950 5348 5622 5769 6180 4950 Size: 398 274 147 411 5287 Fragments arranged by size: 5287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2691 2721 1178 Size: 30 165 30 1288 30 4974 Fragments arranged by size: 4974 1288 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6517 BseYI C'CCAG_C Cuts at: 2008 2165 2499 3048 3484 3615 5720 6216 2008 Size: 157 334 549 436 131 2105 496 2309 Fragments arranged by size: 2309 2105 549 496 436 334 157 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 2491 2849 2967 1163 Size: 573 344 411 358 118 4713 Fragments arranged by size: 4713 573 411 358 344 118 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4728 5756 1540 Size: 275 186 1966 761 1028 2301 Fragments arranged by size: 2301 1966 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4578 4663 5734 4578 Size: 85 1071 5361 Fragments arranged by size: 5361 1071 85 BsmI GAATG_Cn' Cuts at: 4028 4121 4028 Size: 93 6424 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2671 2808 3836 4578 4663 5734 Size: 399 606 275 137 1028 742 85 1071 Cuts at: 5734 1391 Size: 2174 Fragments arranged by size: 2174 1071 1028 742 606 399 275 137 85 BspEI T'CCGG_A Cuts at: 6180 6180 Size: 6517 BspHI T'CATG_A Cuts at: 2124 4406 6136 2124 Size: 2282 1730 2505 Fragments arranged by size: 2505 2282 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2180 2989 3872 844 Size: 1336 809 883 3489 Fragments arranged by size: 3489 1336 883 809 BsrBI CCG'CTC Cuts at: 1394 1823 2742 4404 1394 Size: 429 919 1662 3507 Fragments arranged by size: 3507 1662 919 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5012 5186 65 Size: 1183 3764 174 1396 Fragments arranged by size: 3764 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5161 5348 1700 Size: 110 3351 187 2869 Fragments arranged by size: 3351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 2670 4577 5589 2670 Size: 1907 1012 3598 Fragments arranged by size: 3598 1907 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 4846 BstBI TT'CG_AA Cuts at: 5321 5321 Size: 6517 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6517 BstXI CCAn_nnnn'nTGG Cuts at: 3234 3300 3492 3234 Size: 66 192 6259 Fragments arranged by size: 6259 192 66 BstYI r'GATC_y Cuts at: 1972 2271 2308 3261 3847 4214 4599 4616 6068 Size: 299 37 953 586 367 385 17 1452 Cuts at: 6068 6154 6166 6512 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 953 586 385 367 346 299 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 3373 3373 Size: 6517 BtgI C'CryG_G Cuts at: 513 1065 2388 2421 2918 513 Size: 552 1323 33 497 4112 Fragments arranged by size: 4112 1323 552 497 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5778 Fragments arranged by size: 5778 683 56 BtsI GCAGTG_nn' Cuts at: 2092 4033 4827 4847 2092 Size: 1941 794 20 3762 Fragments arranged by size: 3762 1941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 2652 3198 3821 2652 Size: 546 623 5348 Fragments arranged by size: 5348 623 546 DraIII CAC_nnn'GTG Cuts at: 2109 2514 2109 Size: 405 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5524 809 Size: 507 4208 1802 Fragments arranged by size: 4208 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2817 2915 3964 Size: 54 1425 50 275 18 987 98 1049 Cuts at: 3964 4853 6482 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1049 987 889 275 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2974 3681 4447 1353 Size: 1621 707 766 3423 Fragments arranged by size: 3423 1621 766 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5104 5478 5624 387 Size: 1064 138 3515 374 146 1280 Fragments arranged by size: 3515 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2398 4580 5177 5964 6232 1239 Size: 393 766 2182 597 787 268 1524 Fragments arranged by size: 2182 1524 787 766 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3359 3680 3359 Size: 321 6196 EcoO109I rG'GnC_Cy Cuts at: 2169 3309 3597 2169 Size: 1140 288 5089 Fragments arranged by size: 5089 1140 288 EcoRI G'AATT_C Cuts at: 3826 4204 3826 Size: 378 6139 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 2327 Size: 6517 FspI TGC'GCA Cuts at: 5023 5023 Size: 6517 HaeII r_GCGC'y Cuts at: 1678 5664 1678 Size: 3986 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2339 2371 2691 Size: 32 135 32 671 32 64 32 320 Cuts at: 2691 2723 3369 3401 5158 5190 5232 5264 1373 Size: 32 646 32 1757 32 42 32 2626 Fragments arranged by size: 2626 1757 671 646 320 135 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2788 3084 3861 4107 669 Size: 988 281 850 296 777 246 3079 Fragments arranged by size: 3079 988 850 777 296 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6517 HpaI GTT'AAC Cuts at: 2788 3084 4107 2788 Size: 296 1023 5198 Fragments arranged by size: 5198 1023 296 KpnI G_GTAC'C Cuts at: 3214 3842 3214 Size: 628 5889 MfeI C'AATT_G Cuts at: 4116 4116 Size: 6517 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5631 5815 1305 Size: 4326 184 2007 Fragments arranged by size: 4326 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2819 2917 10 Size: 54 1425 343 987 98 3610 Fragments arranged by size: 3610 1425 987 343 98 54 MslI CAynn'nnrTG Cuts at: 518 1166 3629 4475 4834 4993 518 Size: 648 2463 846 359 159 2042 Fragments arranged by size: 2463 2042 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2679 3933 4610 5758 6003 1606 Size: 1073 1254 677 1148 245 2120 Fragments arranged by size: 2120 1254 1148 1073 677 245 NcoI C'CATG_G Cuts at: 513 1065 2388 2421 2918 513 Size: 552 1323 33 497 4112 Fragments arranged by size: 4112 1323 552 497 33 NdeI CA'TA_TG Cuts at: 387 2636 387 Size: 2249 4268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 2023 2786 3538 3875 5420 1132 Size: 97 794 763 752 337 1545 2229 Fragments arranged by size: 2229 1545 794 763 752 337 97 PasI CC'CwG_GG Cuts at: 1348 2076 1348 Size: 728 5789 PciI A'CATG_T Cuts at: 5416 5416 Size: 6517 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6517 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2205 4586 4618 6099 6131 2173 Size: 32 2381 32 1481 32 2559 Fragments arranged by size: 2559 2381 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2169 2169 Size: 6517 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6517 PsiI TTA'TAA Cuts at: 2580 3551 4087 2580 Size: 971 536 5010 Fragments arranged by size: 5010 971 536 PstI C_TGCA'G Cuts at: 830 3595 3869 830 Size: 2765 274 3478 Fragments arranged by size: 3478 2765 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SbfI CC_TGCA'GG Cuts at: 3869 3869 Size: 6517 ScaI AGT'ACT Cuts at: 2429 3567 4765 6477 2429 Size: 1138 1198 1712 2469 Fragments arranged by size: 2469 1712 1198 1138 SfcI C'TryA_G Cuts at: 826 1045 2431 3426 3591 3865 5000 5681 5872 Size: 219 1386 995 165 274 1135 681 191 Cuts at: 5872 826 Size: 1471 Fragments arranged by size: 1471 1386 1135 995 681 274 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6517 SmaI CCC'GGG Cuts at: 1872 3844 1872 Size: 1972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4621 5522 5784 820 Size: 197 926 2678 901 262 1553 Fragments arranged by size: 2678 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4308 493 Size: 3815 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6517 SphI G_CATG'C Cuts at: 1132 3875 1132 Size: 2743 3774 SspI AAT'ATT Cuts at: 5 52 3104 3476 4441 5 Size: 47 3052 372 965 2081 Fragments arranged by size: 3052 2081 965 372 47 StuI AGG'CCT Cuts at: 1517 3059 1517 Size: 1542 4975 StyI C'CwwG_G Cuts at: 513 1065 2176 2325 2388 2421 2814 2918 3091 Size: 552 1111 149 63 33 393 104 173 Cuts at: 3091 3956 513 Size: 865 3074 Fragments arranged by size: 3074 1111 865 552 393 173 149 104 63 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 3183 4544 4561 4714 4899 5344 Size: 1255 534 1069 1361 17 153 185 445 Cuts at: 5344 325 Size: 1498 Fragments arranged by size: 1498 1361 1255 1069 534 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2186 2427 3316 Size: 274 80 33 51 1443 209 241 889 Cuts at: 3316 3565 4763 6475 96 Size: 249 1198 1712 138 Fragments arranged by size: 1712 1443 1198 889 274 249 241 209 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3430 3462 6477 6509 3430 Size: 32 3015 32 3438 Fragments arranged by size: 3438 3015 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6517 XbaI T'CTAG_A Cuts at: 3853 3853 Size: 6517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3804 3804 Size: 6517 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3842 1870 Size: 1972 4545 XmnI GAAnn'nnTTC Cuts at: 4646 4646 Size: 6517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4325 276 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI