################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AgeI 332 ACCGGT A^CCGGT BanI 463 GGTGCC G^G[CT][GA]CC BbvI 480 GCAGC GCAGC(8/12) BfaI 442 CTAG C^TAG BsaJI 299 CCCTGG C^C[ACGT][ACGT]GG BsaWI 332 ACCGGT [AT]^CCGG[AT] BsiYI 274 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 274 CCTGGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 70 GAGAC GTCTC(1/5) BspMI 269 ACCTGC ACCTGC(4/8) BsrBI 526 GAGCGG CCGCTC(-3/-3) BsrDI 128 GCAATG GCAATG(2/0) BsrFI 332 ACCGGT [GA]^CCGG[CT] BsrI 97 CCAGT ACTGG(1/-1) BstAPI 480 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 158 TTCGAA TT^CGAA BstF5I 134 GGATG GGATG(2/0) BstXI 293 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 387 GCTTGC GC[ACGT]^[ACGT]GC Cfr10I 332 ACCGGT [GA]^CCGG[CT] DraII 266 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 237 CTCTTC CTCTTC(1/4) EcoNI 274 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 266 GGGACCT [GA]G^G[ACGT]CC[CT] FauI 528 GCGGG CCCGC(4/6) Fnu4HI 480 GCAGC GC^[ACGT]GC FokI 134 GGATG GGATG(9/13) HaeIII 183 GGCC GG^CC HapII 333 CCGG C^CGG HincII 100 GTTAAC GT[CT]^[GA]AC HindII 100 GTTAAC GT[CT]^[GA]AC HpaI 100 GTTAAC GTT^AAC HpaII 333 CCGG C^CGG ItaI 480 GCAGC GC^[ACGT]GC Ksp632I 237 CTCTTC CTCTTC(1/4) MaeI 442 CTAG C^TAG MseI 101 TTAA T^TAA MslI 105 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 398 CCGCTG C[AC]G^C[GT]G MspI 333 CCGG C^CGG NspV 158 TTCGAA TT^CGAA PinAI 332 ACCGGT A^CCGGT PpuMI 266 GGGACCT [GA]G^G[AT]CC[CT] PstI 487 CTGCAG CTGCA^G SfcI 487 CTGCAG C^T[GA][CT]AG SfuI 158 TTCGAA TT^CGAA StuI 182 AGGCCT AGG^CCT TaqI 159 TCGA T^CGA TfiI 375 GAATC G^A[AT]TC Tru9I 101 TTAA T^TAA TseI 480 GCAGC G^C[AT]GC Tsp509I 365 AATT ^AATT TspRI 150 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 159 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 398 CCGC CCGC(-3/-1) 528 GCGG AlwNI 281 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 481 CAGCTACTG BmyI 152 GTGCCC G[AGT]GC[ACT]^C 464 GTGCCC BpmI 517 CTCCAG CTGGAG(16/14) 301 CTGGAG BsgI 359 CTGCAC GTGCAG(16/14) 478 GTGCAG BsmFI 119 GGGAC GGGAC(10/14) 266 GGGAC Bsp1286I 152 GTGCCC G[AGT]GC[ACT]^C 464 GTGCCC DpnII 54 GATC ^GATC 492 GATC HphI 51 GGTGA GGTGA(8/7) 187 TCACC MboI 54 GATC ^GATC 492 GATC MboII 78 GAAGA GAAGA(8/7) 238 TCTTC MwoI 391 GCCAAAACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 480 GCAGCTACTGC NdeII 54 GATC ^GATC 492 GATC NlaIV 266 GGGACC GG[ACGT]^[ACGT]CC 463 GGTGCC PleI 72 GACTC GAGTC(4/5) 121 GACTC Sau3AI 54 GATC ^GATC 492 GATC SfaNI 384 GATGC GCATC(5/9) 355 GCATC XcmI 290 CCACCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 437 CCAAGCTAGACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI AvaII BbvCI BssKI BstNI Cfr13I DdeI EcoRII HinfI MnlI MvaI NlaIII Sau96I ScrFI