################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1038 GACGTC GACGT^C AlwNI 1061 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 577 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 37 GGCACC G^G[CT][GA]CC BbsI 823 GTCTTC GAAGAC(2/6) BcnI 177 CCGGG CC^[GC]GG BmyI 36 GGGCAC G[AGT]GC[ACT]^C BpmI 855 CTCCAG CTGGAG(16/14) BpuAI 823 GTCTTC GAAGAC(2/6) BsaJI 497 CCTGGG C^C[ACGT][ACGT]GG BseRI 412 GAGGAG GAGGAG(10/8) BsmAI 638 GAGAC GTCTC(1/5) Bsp1286I 36 GGGCAC G[AGT]GC[ACT]^C BspHI 363 TCATGA T^CATGA BspMI 468 ACCTGC ACCTGC(4/8) BsrDI 258 CATTGC GCAATG(2/0) BsrI 857 CCAGT ACTGG(1/-1) BstXI 743 CCATGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 372 TTTAAA TTT^AAA EarI 844 CTCTTC CTCTTC(1/4) EcoNI 405 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1024 GAATTC G^AATTC HgaI 284 GCGTC GACGC(5/10) HinfI 1063 GAATC G^A[ACGT]TC HphI 494 TCACC GGTGA(8/7) Ksp632I 844 CTCTTC CTCTTC(1/4) MslI 945 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 787 CAGCTG C[AC]G^C[GT]G NciI 177 CCGGG CC^[GC]GG PshAI 64 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 696 CTGCAG CTGCA^G PvuII 787 CAGCTG CAG^CTG RcaI 363 TCATGA T^CATGA SapI 843 GCTCTTC GCTCTTC(1/4) SfcI 696 CTGCAG C^T[GA][CT]AG SmlI 1010 CTCAAG C^T[CT][GA]AG SspI 677 AATATT AAT^ATT TaqI 540 TCGA T^CGA TfiI 1063 GAATC G^A[AT]TC Tsp45I 959 GTCAC ^GT[GC]AC TthHB8I 540 TCGA T^CGA XmnI 577 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 283 GGCGTC G[GA]^CG[CT]C 1038 GACGTC AfaI 159 GTAC GT^AC 777 GTAC BsaHI 283 GGCGTC G[GA]^CG[CT]C 1038 GACGTC BsgI 757 CTGCAC GTGCAG(16/14) 790 CTGCAC DdeI 316 CTGAG C^T[ACGT]AG 634 CTAAG DpnII 331 GATC ^GATC 747 GATC HapII 177 CCGG C^CGG 840 CCGG Hin1I 283 GGCGTC G[GA]^CG[CT]C 1038 GACGTC HpaII 177 CCGG C^CGG 840 CCGG MaeII 232 ACGT A^CGT 1039 ACGT MaeIII 137 GTAAC ^GT[ACGT]AC 959 GTCAC MboI 331 GATC ^GATC 747 GATC MseI 373 TTAA T^TAA 445 TTAA MspI 177 CCGG C^CGG 840 CCGG MwoI 193 GCATACAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 759 GCACCTAAAGC NdeII 331 GATC ^GATC 747 GATC NlaIV 37 GGCACC GG[ACGT]^[ACGT]CC 500 GGGTCC RsaI 159 GTAC GT^AC 777 GTAC Sau3AI 331 GATC ^GATC 747 GATC Tru9I 373 TTAA T^TAA 445 TTAA XcmI 23 CCATCTGTAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 487 CCAGAACTCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BbvI BsiYI BslI BssKI BstF5I BstNI Cfr13I EcoRII Fnu4HI FokI HaeIII ItaI MboII MnlI MvaI NlaIII NspI Sau96I ScrFI SfaNI TseI Tsp509I TspRI