################################## # NO CUT ENZYMES ( 209 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI Bst1107I BstAPI BstEII BstF5I BstNI BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvaI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcyI 82 GGCGTC G[GA]^CG[CT]C AfaI 30 GTAC GT^AC AluI 152 AGCT AG^CT AvaII 50 GGACC G^G[AT]CC BsaHI 82 GGCGTC G[GA]^CG[CT]C BsiEI 5 CGGCCG CG[GA][CT]^CG BssHII 99 GCGCGC G^CGCGC BstBI 21 TTCGAA TT^CGAA BstXI 71 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 99 GCGCGC GC[ACGT]^[ACGT]GC Cfr13I 50 GGACC G^G[ACGT]CC DpnII 109 GATC ^GATC DraII 49 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 5 CGGCCG [CT]^GGCC[GA] EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG EcoO109I 49 AGGACCT [GA]G^G[ACGT]CC[CT] HaeIII 6 GGCC GG^CC Hin1I 82 GGCGTC G[GA]^CG[CT]C HincII 15 GTTGAC GT[CT]^[GA]AC HindII 15 GTTGAC GT[CT]^[GA]AC HphI 68 TCACC GGTGA(8/7) MaeII 13 ACGT A^CGT MboI 109 GATC ^GATC MboII 92 TCTTC GAAGA(8/7) NdeII 109 GATC ^GATC NlaIII 87 CATG CATG^ NotI 4 GCGGCCGC GC^GGCCGC NspV 21 TTCGAA TT^CGAA PpuMI 49 AGGACCT [GA]G^G[AT]CC[CT] RsaI 30 GTAC GT^AC Sau3AI 109 GATC ^GATC Sau96I 50 GGACC G^G[ACGT]CC SfuI 21 TTCGAA TT^CGAA ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### Fnu4HI 4 GCGGC GC^[ACGT]GC 7 GCCGC HgaI 121 GACGC GACGC(5/10) 83 GCGTC ItaI 4 GCGGC GC^[ACGT]GC 7 GCCGC MslI 72 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 172 CACAAGTATG ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AccII AciI BstUI CfoI HhaI HinP1I MnlI MvnI TaqI TthHB8I