Restriction map for
pFN21AE9680
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsgI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
MamI MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 1568 GTCGAC GT^[AC][GT]AC
AcyI 34 GACGCC G[GA]^CG[CT]C
AhdI 1873 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1177 GATCC GGATC(4/5)
AocI 229 CCTGAGG CC^T[ACGT]AGG
AspEI 1873 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1857 GGGCTC G[GA]GC[CT]^C
BclI 1076 TGATCA T^GATCA
BfaI 322 CTAG C^TAG
BsaHI 34 GACGCC G[GA]^CG[CT]C
BsaWI 1161 ACCGGA [AT]^CCGG[AT]
BsmI 1824 GCATTC GAATGC(1/-1)
Bsu36I 229 CCTGAGG CC^T[ACGT]AGG
DsaI 60 CCGCGG C^C[GA][CT]GG
Eam1105I 1873 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 229 CCTGAGG CC^T[ACGT]AGG
FbaI 1076 TGATCA T^GATCA
Hin1I 34 GACGCC G[GA]^CG[CT]C
KspI 60 CCGCGG CCGC^GG
MaeI 322 CTAG C^TAG
NspI 71 ACATGC [GA]CATG^[CT]
PleI 1894 GACTC GAGTC(4/5)
PvuII 2075 CAGCTG CAG^CTG
SacII 60 CCGCGG CCGC^GG
SalI 1568 GTCGAC G^TCGAC
SspI 1545 AATATT AAT^ATT
XbaI 321 TCTAGA T^CTAGA
XcmI 731 CCATAACAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 38 enzymes) #
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AccII 23 CGCG CG^CG
61 CGCG
AlwNI 1676 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
2285 CAGTTGCTG
AspI 88 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1408 GACCCAGTC
BalI 1980 TGGCCA TGG^CCA
2100 TGGCCA
BcnI 105 CCGGG CC^[GC]GG
128 CCGGG
BpmI 893 CTCCAG CTGGAG(16/14)
2219 CTCCAG
BseRI 1229 CTCCTC GAGGAG(10/8)
142 GAGGAG
BsmBI 93 CGTCTC CGTCTC(1/5)
711 GAGACG
Bsp1407I 266 TGTACA T^GTACA
2079 TGTACA
BsrDI 1067 CATTGC GCAATG(2/0)
1303 CATTGC
BsrGI 266 TGTACA T^GTACA
2079 TGTACA
BstNI 1642 CCAGG CC^[AT]GG
2065 CCAGG
BstUI 23 CGCG CG^CG
61 CGCG
BstXI 125 CCACCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2039 CCACCAATATGG
EaeI 1980 TGGCCA [CT]^GGCC[GA]
2100 TGGCCA
EcoRII 1642 CCAGG ^CC[AT]GG
2065 CCAGG
EcoT14I 1588 CCAAGG C^C[AT][AT]GG
1964 CCAAGG
FauI 24 GCGGG CCCGC(4/6)
1206 GCGGG
HaeII 27 GGCGCT [GA]GCGC^[CT]
1903 AGCGCC
HgaI 34 GACGC GACGC(5/10)
92 GCGTC
HincII 247 GTCAAC GT[CT]^[GA]AC
1568 GTCGAC
HindII 247 GTCAAC GT[CT]^[GA]AC
1568 GTCGAC
HphI 534 GGTGA GGTGA(8/7)
838 GGTGA
MaeII 245 ACGT A^CGT
543 ACGT
MluNI 1980 TGGCCA TGG^CCA
2100 TGGCCA
MscI 1980 TGGCCA TGG^CCA
2100 TGGCCA
MvaI 1642 CCAGG CC^[AT]GG
2065 CCAGG
MvnI 23 CGCG CG^CG
61 CGCG
NciI 105 CCGGG CC^[GC]GG
128 CCGGG
PflFI 88 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1408 GACCCAGTC
SapI 1738 GAAGAGC GCTCTTC(1/4)
1457 GCTCTTC
SmlI 1632 CTCAAG C^T[CT][GA]AG
1860 CTCAAG
SspBI 266 TGTACA T^GTACA
2079 TGTACA
StyI 1588 CCAAGG C^C[AT][AT]GG
1964 CCAAGG
TaqI 660 TCGA T^CGA
1569 TCGA
Tsp45I 1088 GTGAC ^GT[GC]AC
2416 GTGAC
Tth111I 88 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1408 GACCCAGTC
TthHB8I 660 TCGA T^CGA
1569 TCGA
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
ApaLI ApoI AspHI AvaII BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I Cac8I CfoI Cfr13I DdeI
DpnII EarI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MwoI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp509I
TspRI