(Circular) (Six-base) MAPSORT of: pFN21AE9680.seq Check: 7421 from: 1 to: 7126 pFN21AE9680 7126 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4936 278 Size: 53 83 186 936 3400 2468 Fragments arranged by size: 3400 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3574 4469 1937 Size: 1637 895 4594 Fragments arranged by size: 4594 1637 895 Acc65I G'GTAC_C Cuts at: 4447 4447 Size: 7126 AclI AA'CG_TT Cuts at: 1563 5253 5626 1563 Size: 3690 373 3063 Fragments arranged by size: 3690 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3791 4002 4239 5189 6573 6841 1632 Size: 2159 211 237 950 1384 268 1917 Fragments arranged by size: 2159 1917 1384 950 268 237 211 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6929 AflIII A'CryG_T Cuts at: 1203 6025 7002 1203 Size: 4822 977 1327 Fragments arranged by size: 4822 1327 977 AgeI A'CCGG_T Cuts at: 5957 5957 Size: 7126 AhdI GACnn_n'nnGTC Cuts at: 3883 5855 3883 Size: 1972 5154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2093 3813 1184 Size: 909 1720 4497 Fragments arranged by size: 4497 1720 909 AlwNI CAG_nnn'CTG Cuts at: 3686 4295 4869 6441 3686 Size: 609 574 1572 4371 Fragments arranged by size: 4371 1572 609 574 ApaLI G'TGCA_C Cuts at: 3939 4141 4196 5183 6339 3939 Size: 202 55 987 1156 4726 Fragments arranged by size: 4726 1156 987 202 55 ApoI r'AATT_y Cuts at: 1493 2723 3134 3268 3604 4435 4661 4813 6922 Size: 1230 411 134 336 831 226 152 2109 Cuts at: 6922 1493 Size: 1697 Fragments arranged by size: 2109 1697 1230 831 411 336 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 5680 160 Size: 872 4648 1606 Fragments arranged by size: 4648 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7126 AvaI C'yCGr_G Cuts at: 1870 1943 4451 1870 Size: 73 2508 4545 Fragments arranged by size: 4545 2508 73 BamHI G'GATC_C Cuts at: 4456 4823 4456 Size: 367 6759 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4447 5902 618 Size: 325 217 627 2660 1455 1842 Fragments arranged by size: 2660 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3866 4445 1391 Size: 2475 579 4072 Fragments arranged by size: 4072 2475 579 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5317 5351 1903 Size: 34 3380 34 3678 Fragments arranged by size: 3678 3380 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5018 6228 5018 Size: 1210 5916 BclI T'GATC_A Cuts at: 1548 3081 1548 Size: 1533 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5737 136 Size: 107 122 71 631 605 4065 1525 Fragments arranged by size: 4065 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7121 7121 Size: 7126 BlpI GC'TnA_GC Cuts at: 4543 4543 Size: 7126 Bme1580I G_kGCm'C Cuts at: 1790 2121 3943 4145 4200 5187 6343 1790 Size: 331 1822 202 55 987 1156 2573 Fragments arranged by size: 2573 1822 1156 987 331 202 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7126 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 3149 3410 Size: 416 53 83 399 24 1128 604 261 Cuts at: 3410 4857 5815 442 Size: 1447 958 1753 Fragments arranged by size: 1753 1447 1128 958 604 416 399 261 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2883 4209 5786 1239 Size: 1644 1326 1577 2579 Fragments arranged by size: 2579 1644 1577 1326 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3622 3850 5251 6116 6414 3622 Size: 228 1401 865 298 4334 Fragments arranged by size: 4334 1401 865 298 228 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5789 882 Size: 617 4290 2219 Fragments arranged by size: 4290 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4917 493 Size: 711 3713 2702 Fragments arranged by size: 3713 2702 711 BsaBI GATnn'nnATC Cuts at: 7120 7120 Size: 7126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 4933 5315 5950 Size: 53 83 186 936 507 2893 382 635 Cuts at: 5950 275 Size: 1451 Fragments arranged by size: 2893 1451 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3166 5559 5957 6231 6378 6789 3166 Size: 2393 398 274 147 411 3503 Fragments arranged by size: 3503 2393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 6190 Fragments arranged by size: 6190 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 3225 1186 Size: 976 1063 5087 Fragments arranged by size: 5087 1063 976 BseYI C'CCAG_C Cuts at: 3801 4132 4328 6329 6825 3801 Size: 331 196 2001 496 4102 Fragments arranged by size: 4102 2001 496 331 196 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6553 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4576 5337 6365 1540 Size: 275 186 2575 761 1028 2301 Fragments arranged by size: 2575 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3943 4145 4200 5187 5272 6343 3943 Size: 202 55 987 85 1071 4726 Fragments arranged by size: 4726 1071 987 202 85 55 BsmI GAATG_Cn' Cuts at: 3829 4637 4730 3829 Size: 808 93 6225 Fragments arranged by size: 6225 808 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2104 2710 2104 Size: 606 6520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 3866 3943 4145 4200 4445 5187 Size: 399 331 1745 77 202 55 245 742 Cuts at: 5187 5272 6343 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1745 1071 742 399 331 245 202 85 77 55 BspEI T'CCGG_A Cuts at: 6789 6789 Size: 7126 BspHI T'CATG_A Cuts at: 5015 6745 5015 Size: 1730 5396 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4481 844 Size: 3637 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5013 1394 Size: 429 3190 3507 Fragments arranged by size: 3507 3190 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3071 3307 5621 5795 65 Size: 1183 1823 236 2314 174 1396 Fragments arranged by size: 2314 1823 1396 1183 236 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5770 5957 1700 Size: 110 3960 187 2869 Fragments arranged by size: 3960 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2271 4084 96 Size: 2175 1813 3138 Fragments arranged by size: 3138 2175 1813 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7126 BssSI C'ACGA_G Cuts at: 5186 6198 5186 Size: 1012 6114 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7126 BstBI TT'CG_AA Cuts at: 5930 5930 Size: 7126 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7126 BstXI CCAn_nnnn'nTGG Cuts at: 2137 4051 2137 Size: 1914 5212 BstYI r'GATC_y Cuts at: 1972 4456 4823 5208 5225 6677 6763 6775 7121 Size: 2484 367 385 17 1452 86 12 346 Cuts at: 7121 1972 Size: 1977 Fragments arranged by size: 2484 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2235 2235 Size: 7126 BtgI C'CryG_G Cuts at: 513 1065 2065 513 Size: 552 1000 5574 Fragments arranged by size: 5574 1000 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4248 487 Size: 683 56 3022 3365 Fragments arranged by size: 3365 3022 683 56 BtsI GCAGTG_nn' Cuts at: 3936 3964 4200 4339 4642 5436 5456 3936 Size: 28 236 139 303 794 20 5606 Fragments arranged by size: 5606 794 303 236 139 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7126 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3129 3164 529 Size: 35 2565 35 4491 Fragments arranged by size: 4491 2565 35 35 DraI TTT'AAA Cuts at: 4430 4430 Size: 7126 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6133 809 Size: 507 4817 1802 Fragments arranged by size: 4817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3985 4105 4573 Size: 54 1425 50 275 18 2155 120 468 Cuts at: 4573 5462 7091 8 Size: 889 1629 43 Fragments arranged by size: 2155 1629 1425 889 468 275 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4573 1537 Size: 275 2761 4090 Fragments arranged by size: 4090 2761 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2758 2913 2973 3469 3738 5056 1353 Size: 1405 155 60 496 269 1318 3423 Fragments arranged by size: 3423 1405 1318 496 269 155 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5713 6087 6233 387 Size: 1064 138 4124 374 146 1280 Fragments arranged by size: 4124 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2883 3791 4002 4209 4239 5189 5786 Size: 393 1251 908 211 207 30 950 597 Cuts at: 5786 6573 6841 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1251 950 908 787 597 393 268 211 207 30 EcoRI G'AATT_C Cuts at: 4435 4813 4435 Size: 378 6748 FspI TGC'GCA Cuts at: 5632 5632 Size: 7126 HaeII r_GCGC'y Cuts at: 1678 2036 3912 6273 1678 Size: 358 1876 2361 2531 Fragments arranged by size: 2531 2361 1876 358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2138 2170 2675 Size: 32 135 32 512 32 22 32 505 Cuts at: 2675 2707 3034 3066 3383 3415 3869 3901 5767 Size: 32 327 32 317 32 454 32 1866 Cuts at: 5767 5799 5841 5873 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1866 512 505 454 327 317 135 42 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 2254 3575 4470 4716 669 Size: 988 281 316 1321 895 246 3079 Fragments arranged by size: 3079 1321 988 895 316 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7126 HpaI GTT'AAC Cuts at: 4716 4716 Size: 7126 KpnI G_GTAC'C Cuts at: 4451 4451 Size: 7126 MfeI C'AATT_G Cuts at: 4725 4725 Size: 7126 MluI A'CGCG_T Cuts at: 7002 7002 Size: 7126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6240 6424 1305 Size: 4935 184 2007 Fragments arranged by size: 4935 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3987 4107 10 Size: 54 1425 343 2155 120 3029 Fragments arranged by size: 3029 2155 1425 343 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3261 4049 5084 5443 5602 518 Size: 648 969 1126 788 1035 359 159 2042 Fragments arranged by size: 2042 1126 1035 969 788 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 2067 4082 4542 5219 6367 6612 1606 Size: 447 14 2015 460 677 1148 245 2120 Fragments arranged by size: 2120 2015 1148 677 460 447 245 14 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6574 NdeI CA'TA_TG Cuts at: 387 387 Size: 7126 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7126 NotI GC'GGCC_GC Cuts at: 4573 4573 Size: 7126 NspI r_CATG'y Cuts at: 1132 1229 2080 4484 6029 1132 Size: 97 851 2404 1545 2229 Fragments arranged by size: 2404 2229 1545 851 97 PasI CC'CwG_GG Cuts at: 1348 4070 1348 Size: 2722 4404 PciI A'CATG_T Cuts at: 6025 6025 Size: 7126 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7126 PmeI GTTT'AAAC Cuts at: 4430 4430 Size: 7126 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5195 5227 6708 6740 5195 Size: 32 1481 32 5581 Fragments arranged by size: 5581 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7126 PsiI TTA'TAA Cuts at: 4696 4696 Size: 7126 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2151 2183 2151 Size: 32 7094 PstI C_TGCA'G Cuts at: 830 2561 3682 3931 4478 830 Size: 1731 1121 249 547 3478 Fragments arranged by size: 3478 1731 1121 547 249 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7126 PvuII CAG'CTG Cuts at: 4082 4082 Size: 7126 SacII CC_GC'GG Cuts at: 2068 2068 Size: 7126 SalI G'TCGA_C Cuts at: 1936 3573 4468 1936 Size: 1637 895 4594 Fragments arranged by size: 4594 1637 895 SapI GCTCTTCn'nnn_ Cuts at: 3469 3738 3469 Size: 269 6857 SbfI CC_TGCA'GG Cuts at: 4478 4478 Size: 7126 ScaI AGT'ACT Cuts at: 5374 7086 5374 Size: 1712 5414 SfcI C'TryA_G Cuts at: 826 1045 2557 3474 3578 3678 3687 3927 4474 Size: 219 1512 917 104 100 9 240 547 Cuts at: 4474 5609 6290 6481 826 Size: 1135 681 191 1471 Fragments arranged by size: 1512 1471 1135 917 681 547 240 219 191 104 100 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7126 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7126 SmaI CCC'GGG Cuts at: 1872 4453 1872 Size: 2581 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 3865 5230 6131 6393 820 Size: 197 926 1694 228 1365 901 262 1553 Fragments arranged by size: 1694 1553 1365 926 901 262 228 197 SnaBI TAC'GTA Cuts at: 493 4917 493 Size: 4424 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7126 SphI G_CATG'C Cuts at: 1132 4484 1132 Size: 3352 3774 SspI AAT'ATT Cuts at: 5 52 3552 5050 5 Size: 47 3500 1498 2081 Fragments arranged by size: 3500 2081 1498 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7126 StyI C'CwwG_G Cuts at: 513 1065 3593 3969 4565 513 Size: 552 2528 376 596 3074 Fragments arranged by size: 3074 2528 596 552 376 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2527 3408 5153 5170 5323 5508 5953 Size: 1255 947 881 1745 17 153 185 445 Cuts at: 5953 325 Size: 1498 Fragments arranged by size: 1745 1498 1255 947 881 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 4084 5372 Size: 274 80 33 51 1443 294 1813 1288 Cuts at: 5372 7084 96 Size: 1712 138 Fragments arranged by size: 1813 1712 1443 1288 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3312 3344 4135 4167 7086 7118 3312 Size: 32 791 32 2919 32 3320 Fragments arranged by size: 3320 2919 791 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2096 3416 1316 Size: 780 1320 5026 Fragments arranged by size: 5026 1320 780 XbaI T'CTAG_A Cuts at: 2326 4462 2326 Size: 2136 4990 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2743 2743 Size: 7126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7126 XmaI C'CCGG_G Cuts at: 1870 4451 1870 Size: 2581 4545 XmnI GAAnn'nnTTC Cuts at: 5255 5255 Size: 7126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4934 276 Size: 53 83 186 936 3400 2468 Fragments arranged by size: 3400 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI