(Linear) (Six-base) MAPSORT of: KIEE9680.seq Check: 5689 from: 1 to: 2421 KIEE9680.orf With 182 enzymes: SgfI * December 5, 2014 13:30 .. AccI GT'mk_AC Cuts at: 0 1569 2421 Size: 1569 852 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1786 1997 2234 2421 Size: 1786 211 237 187 Fragments arranged by size: 1786 237 211 187 AhdI GACnn_n'nnGTC Cuts at: 0 1878 2421 Size: 1878 543 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 88 1808 2421 Size: 88 1720 613 Fragments arranged by size: 1720 613 88 AlwNI CAG_nnn'CTG Cuts at: 0 1681 2290 2421 Size: 1681 609 131 Fragments arranged by size: 1681 609 131 ApaLI G'TGCA_C Cuts at: 0 1934 2136 2191 2421 Size: 1934 202 55 230 Fragments arranged by size: 1934 230 202 55 ApoI r'AATT_y Cuts at: 0 718 1129 1263 1599 2421 Size: 718 411 134 336 822 Fragments arranged by size: 822 718 411 336 134 BanII G_rGCy'C Cuts at: 0 1861 2421 Size: 1861 560 BclI T'GATC_A Cuts at: 0 1076 2421 Size: 1076 1345 Bme1580I G_kGCm'C Cuts at: 0 116 1938 2140 2195 2421 Size: 116 1822 202 55 226 Fragments arranged by size: 1822 226 202 116 55 BmrI ACTGGGnnnn_n' Cuts at: 0 540 1144 1405 2421 Size: 540 604 261 1016 Fragments arranged by size: 1016 604 540 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 878 2204 2421 Size: 878 1326 217 Fragments arranged by size: 1326 878 217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1617 1845 2421 Size: 1617 228 576 Fragments arranged by size: 1617 576 228 BsaHI Gr'CG_yC Cuts at: 0 35 2421 Size: 35 2386 BsaWI w'CCGG_w Cuts at: 0 1161 2421 Size: 1161 1260 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 79 109 2421 Size: 79 30 2312 Fragments arranged by size: 2312 79 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 157 1220 2421 Size: 157 1063 1201 Fragments arranged by size: 1201 1063 157 BseYI C'CCAG_C Cuts at: 0 1796 2127 2323 2421 Size: 1796 331 196 98 Fragments arranged by size: 1796 331 196 98 BsiHKAI G_wGCw'C Cuts at: 0 1938 2140 2195 2421 Size: 1938 202 55 226 Fragments arranged by size: 1938 226 202 55 BsmI GAATG_Cn' Cuts at: 0 1824 2421 Size: 1824 597 BsmBI CGTCTCn'nnnn_ Cuts at: 0 99 705 2421 Size: 99 606 1716 Fragments arranged by size: 1716 606 99 Bsp1286I G_dGCh'C Cuts at: 0 116 1861 1938 2140 2195 2421 Size: 116 1745 77 202 55 226 Fragments arranged by size: 1745 226 202 116 77 55 BsrDI GCAATG_nn' Cuts at: 0 1066 1302 2421 Size: 1066 236 1119 Fragments arranged by size: 1119 1066 236 BsrGI T'GTAC_A Cuts at: 0 266 2079 2421 Size: 266 1813 342 Fragments arranged by size: 1813 342 266 BstXI CCAn_nnnn'nTGG Cuts at: 0 132 2046 2421 Size: 132 1914 375 Fragments arranged by size: 1914 375 132 Bsu36I CC'TnA_GG Cuts at: 0 230 2421 Size: 230 2191 BtgI C'CryG_G Cuts at: 0 60 2421 Size: 60 2361 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2243 2421 Size: 2243 178 BtsI GCAGTG_nn' Cuts at: 0 1931 1959 2195 2334 2421 Size: 1931 28 236 139 87 Fragments arranged by size: 1931 236 139 87 28 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1124 1159 2421 Size: 1124 35 1262 Fragments arranged by size: 1262 1124 35 EaeI y'GGCC_r Cuts at: 0 1980 2100 2421 Size: 1980 120 321 Fragments arranged by size: 1980 321 120 EarI CTCTTCn'nnn_ Cuts at: 0 753 908 968 1464 1733 2421 Size: 753 155 60 496 269 688 Fragments arranged by size: 753 688 496 269 155 60 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 878 1786 1997 2204 2234 2421 Size: 878 908 211 207 30 187 Fragments arranged by size: 908 878 211 207 187 30 HaeII r_GCGC'y Cuts at: 0 31 1907 2421 Size: 31 1876 514 Fragments arranged by size: 1876 514 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 133 165 670 702 1029 1061 Size: 79 32 22 32 505 32 327 32 Cuts at: 1061 1378 1410 1864 1896 2421 Size: 317 32 454 32 525 Fragments arranged by size: 525 505 454 327 317 79 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 249 1570 2421 Size: 249 1321 851 Fragments arranged by size: 1321 851 249 MscI TGG'CCA Cuts at: 0 1982 2102 2421 Size: 1982 120 319 Fragments arranged by size: 1982 319 120 MslI CAynn'nnrTG Cuts at: 0 130 1256 2044 2421 Size: 130 1126 788 377 Fragments arranged by size: 1126 788 377 130 MspA1I CmG'CkG Cuts at: 0 48 62 2077 2421 Size: 48 14 2015 344 Fragments arranged by size: 2015 344 48 14 NspI r_CATG'y Cuts at: 0 75 2421 Size: 75 2346 PasI CC'CwG_GG Cuts at: 0 2065 2421 Size: 2065 356 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 146 178 2421 Size: 146 32 2243 Fragments arranged by size: 2243 146 32 PstI C_TGCA'G Cuts at: 0 556 1677 1926 2421 Size: 556 1121 249 495 Fragments arranged by size: 1121 556 495 249 PvuII CAG'CTG Cuts at: 0 2077 2421 Size: 2077 344 SacII CC_GC'GG Cuts at: 0 63 2421 Size: 63 2358 SalI G'TCGA_C Cuts at: 0 1568 2421 Size: 1568 853 SapI GCTCTTCn'nnn_ Cuts at: 0 1464 1733 2421 Size: 1464 269 688 Fragments arranged by size: 1464 688 269 SfcI C'TryA_G Cuts at: 0 552 1469 1573 1673 1682 1922 2421 Size: 552 917 104 100 9 240 499 Fragments arranged by size: 917 552 499 240 104 100 9 SmlI C'TyrA_G Cuts at: 0 1632 1860 2421 Size: 1632 228 561 Fragments arranged by size: 1632 561 228 SspI AAT'ATT Cuts at: 0 1547 2421 Size: 1547 874 StyI C'CwwG_G Cuts at: 0 1588 1964 2421 Size: 1588 376 457 Fragments arranged by size: 1588 457 376 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 522 1403 2421 Size: 522 881 1018 Fragments arranged by size: 1018 881 522 TatI w'GTAC_w Cuts at: 0 266 2079 2421 Size: 266 1813 342 Fragments arranged by size: 1813 342 266 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1307 1339 2130 2162 2421 Size: 1307 32 791 32 259 Fragments arranged by size: 1307 791 259 32 32 Tth111I GACn'n_nGTC Cuts at: 0 91 1411 2421 Size: 91 1320 1010 Fragments arranged by size: 1320 1010 91 XbaI T'CTAG_A Cuts at: 0 321 2421 Size: 321 2100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 738 2421 Size: 738 1683 Enzymes that do cut: AccI AcuI AhdI AlfI AlwNI ApaLI ApoI BanII BclI Bme1580I BmrI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstXI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI HaeII Hin4I HincII MscI MslI MspA1I NspI PasI PsrI PstI PvuII SacII SalI SapI SfcI SmlI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XhoI XmaI XmnI ZraI