(Circular) (Six-base) MAPSORT of: pFN21AE6753.seq Check: 8296 from: 1 to: 6712 pFN21AE6753 6712 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4055 1937 Size: 2118 4594 Acc65I G'GTAC_C Cuts at: 4033 4033 Size: 6712 AclI AA'CG_TT Cuts at: 1563 4011 4839 5212 1563 Size: 2448 828 373 3063 Fragments arranged by size: 3063 2448 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2010 3477 3551 4775 6159 6427 1632 Size: 378 1467 74 1224 1384 268 1917 Fragments arranged by size: 1917 1467 1384 1224 378 268 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 3612 5611 6588 1203 Size: 2409 1999 977 1327 Fragments arranged by size: 2409 1999 1327 977 AgeI A'CCGG_T Cuts at: 5543 5543 Size: 6712 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6712 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2296 1184 Size: 1112 5600 AlwNI CAG_nnn'CTG Cuts at: 2155 3176 3544 4455 6027 2155 Size: 1021 368 911 1572 2840 Fragments arranged by size: 2840 1572 1021 911 368 ApaLI G'TGCA_C Cuts at: 4769 5925 4769 Size: 1156 5556 ApoI r'AATT_y Cuts at: 1493 2972 3000 3190 3212 3266 3323 4021 4247 Size: 1479 28 190 22 54 57 698 226 Cuts at: 4247 4399 6508 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1479 698 226 190 152 57 54 28 22 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 2567 4037 1870 Size: 73 624 1470 4545 Fragments arranged by size: 4545 1470 624 73 BamHI G'GATC_C Cuts at: 2303 2537 4042 4409 2303 Size: 234 1505 367 4606 Fragments arranged by size: 4606 1505 367 234 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3146 3450 4033 5488 618 Size: 325 217 627 1359 304 583 1455 1842 Fragments arranged by size: 1842 1455 1359 627 583 325 304 217 BanII G_rGCy'C Cuts at: 1391 3362 3773 4031 1391 Size: 1971 411 258 4072 Fragments arranged by size: 4072 1971 411 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2660 928 Size: 971 761 4980 Fragments arranged by size: 4980 971 761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4903 4937 1903 Size: 34 2966 34 3678 Fragments arranged by size: 3678 2966 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 2601 1548 Size: 1053 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6707 6707 Size: 6712 BlpI GC'TnA_GC Cuts at: 2772 3534 4129 2772 Size: 762 595 5355 Fragments arranged by size: 5355 762 595 Bme1580I G_kGCm'C Cuts at: 1790 4773 5929 1790 Size: 2983 1156 2573 Fragments arranged by size: 2983 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6712 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 2775 4443 Size: 416 53 83 399 24 892 466 1668 Cuts at: 4443 5401 442 Size: 958 1753 Fragments arranged by size: 1753 1668 958 892 466 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2531 2881 3588 3777 5372 1239 Size: 905 387 350 707 189 1595 2579 Fragments arranged by size: 2579 1595 905 707 387 350 189 Bpu10I CC'TnA_GC Cuts at: 2090 3455 3767 2090 Size: 1365 312 5035 Fragments arranged by size: 5035 1365 312 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4837 5702 6000 4837 Size: 865 298 5549 Fragments arranged by size: 5549 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2027 2834 2877 5375 882 Size: 617 528 807 43 2498 2219 Fragments arranged by size: 2498 2219 807 617 528 43 BsaAI yAC'GTr Cuts at: 493 1204 4503 493 Size: 711 3299 2702 Fragments arranged by size: 3299 2702 711 BsaBI GATnn'nnATC Cuts at: 3821 6706 3821 Size: 2885 3827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4519 4901 5536 275 Size: 53 83 186 936 2986 382 635 1451 Fragments arranged by size: 2986 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2388 5145 5543 5817 5964 6375 2388 Size: 2757 398 274 147 411 2725 Fragments arranged by size: 2757 2725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3586 3602 3616 3632 1178 Size: 30 165 30 2183 16 14 16 4258 Fragments arranged by size: 4258 2183 165 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2211 3701 1186 Size: 1025 1490 4197 Fragments arranged by size: 4197 1490 1025 BseYI C'CCAG_C Cuts at: 2796 2832 3561 3890 5915 6411 2796 Size: 36 729 329 2025 496 3097 Fragments arranged by size: 3097 2025 729 496 329 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 2603 1163 Size: 573 801 66 5272 Fragments arranged by size: 5272 801 573 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4162 4923 5951 1540 Size: 275 186 2161 761 1028 2301 Fragments arranged by size: 2301 2161 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3773 4773 4858 5929 3773 Size: 1000 85 1071 4556 Fragments arranged by size: 4556 1071 1000 85 BsmI GAATG_Cn' Cuts at: 4223 4316 4223 Size: 93 6619 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3362 3773 4031 4773 4858 5929 1391 Size: 399 1572 411 258 742 85 1071 2174 Fragments arranged by size: 2174 1572 1071 742 411 399 258 85 BspEI T'CCGG_A Cuts at: 6375 6375 Size: 6712 BspHI T'CATG_A Cuts at: 4601 6331 4601 Size: 1730 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4067 844 Size: 3223 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4599 1394 Size: 429 2776 3507 Fragments arranged by size: 3507 2776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2405 5207 5381 65 Size: 1183 1157 2802 174 1396 Fragments arranged by size: 2802 1396 1183 1157 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5356 5543 1700 Size: 110 3546 187 2869 Fragments arranged by size: 3546 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4005 96 Size: 3909 2803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6712 BssSI C'ACGA_G Cuts at: 4772 5784 4772 Size: 1012 5700 BstAPI GCAn_nnn'nTGC Cuts at: 794 3206 794 Size: 2412 4300 BstBI TT'CG_AA Cuts at: 5516 5516 Size: 6712 BstEII G'GTnAC_C Cuts at: 1184 3847 1184 Size: 2663 4049 BstXI CCAn_nnnn'nTGG Cuts at: 2372 2372 Size: 6712 BstYI r'GATC_y Cuts at: 1972 2203 2303 2337 2537 3816 4042 4409 4794 Size: 231 100 34 200 1279 226 367 385 Cuts at: 4794 4811 6263 6349 6361 6707 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1279 385 367 346 231 226 200 100 86 34 17 12 Bsu36I CC'TnA_GG Cuts at: 3854 3854 Size: 6712 BtgI C'CryG_G Cuts at: 513 1065 2004 2900 3019 3924 513 Size: 552 939 896 119 905 3301 Fragments arranged by size: 3301 939 905 896 552 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2738 2962 487 Size: 683 56 1512 224 4237 Fragments arranged by size: 4237 1512 683 224 56 BtsI GCAGTG_nn' Cuts at: 2083 2655 3084 4228 5022 5042 2083 Size: 572 429 1144 794 20 3753 Fragments arranged by size: 3753 1144 794 572 429 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5719 809 Size: 507 4403 1802 Fragments arranged by size: 4403 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2195 2404 4159 Size: 54 1425 50 275 18 365 209 1755 Cuts at: 4159 5048 6677 8 Size: 889 1629 43 Fragments arranged by size: 1755 1629 1425 889 365 275 209 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4159 1537 Size: 275 2347 4090 Fragments arranged by size: 4090 2347 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2629 4642 1353 Size: 1276 2013 3423 Fragments arranged by size: 3423 2013 1276 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3317 5299 5673 5819 387 Size: 1064 138 1728 1982 374 146 1280 Fragments arranged by size: 1982 1728 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3771 3771 Size: 6712 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2144 2531 2881 3477 3551 3588 Size: 393 378 134 387 350 596 74 37 Cuts at: 3588 3777 4775 5372 6159 6427 1239 Size: 189 998 597 787 268 1524 Fragments arranged by size: 1524 998 787 597 596 393 387 378 350 268 189 134 74 37 EcoNI CCTnn'n_nnAGG Cuts at: 2642 3950 2642 Size: 1308 5404 EcoO109I rG'GnC_Cy Cuts at: 2216 3095 3571 3599 2216 Size: 879 476 28 5329 Fragments arranged by size: 5329 879 476 28 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FspI TGC'GCA Cuts at: 5218 5218 Size: 6712 HaeII r_GCGC'y Cuts at: 1678 2860 3712 5859 1678 Size: 1182 852 2147 2531 Fragments arranged by size: 2531 2147 1182 852 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 2586 2618 2938 Size: 32 135 32 669 32 313 32 320 Cuts at: 2938 2970 3337 3369 3602 3634 5353 5385 5427 Size: 32 367 32 233 32 1719 32 42 Cuts at: 5427 5459 1373 Size: 32 2626 Fragments arranged by size: 2626 1719 669 367 320 313 233 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4056 4302 669 Size: 988 281 2118 246 3079 Fragments arranged by size: 3079 2118 988 281 246 HindIII A'AGCT_T Cuts at: 748 2353 748 Size: 1605 5107 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KpnI G_GTAC'C Cuts at: 4037 4037 Size: 6712 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2284 2989 5826 6010 1305 Size: 979 705 2837 184 2007 Fragments arranged by size: 2837 2007 979 705 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2406 10 Size: 54 1425 343 574 4316 Fragments arranged by size: 4316 1425 574 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2981 4670 5029 5188 518 Size: 648 1201 614 1689 359 159 2042 Fragments arranged by size: 2042 1689 1201 648 614 359 159 MspA1I CmG'CkG Cuts at: 1606 2276 2902 3253 3404 4128 4805 5953 6198 Size: 670 626 351 151 724 677 1148 245 Cuts at: 6198 1606 Size: 2120 Fragments arranged by size: 2120 1148 724 677 670 626 351 245 151 NcoI C'CATG_G Cuts at: 513 1065 2004 3019 3924 513 Size: 552 939 1015 905 3301 Fragments arranged by size: 3301 1015 939 905 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 4159 4159 Size: 6712 NspI r_CATG'y Cuts at: 1132 1229 3616 3731 4070 5615 1132 Size: 97 2387 115 339 1545 2229 Fragments arranged by size: 2387 2229 1545 339 115 97 PasI CC'CwG_GG Cuts at: 1348 2826 3362 1348 Size: 1478 536 4698 Fragments arranged by size: 4698 1478 536 PciI A'CATG_T Cuts at: 3612 5611 3612 Size: 1999 4713 PflMI CCAn_nnn'nTGG Cuts at: 1108 2413 1108 Size: 1305 5407 PfoI T'CCnGG_A Cuts at: 2160 2232 3258 2160 Size: 72 1026 5614 Fragments arranged by size: 5614 1026 72 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2216 3095 3599 2216 Size: 879 504 5329 Fragments arranged by size: 5329 879 504 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6712 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PspXI vC'TCGA_Gb Cuts at: 2567 2567 Size: 6712 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2309 2341 2309 Size: 32 6680 PstI C_TGCA'G Cuts at: 830 2131 3091 4064 830 Size: 1301 960 973 3478 Fragments arranged by size: 3478 1301 973 960 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 PvuII CAG'CTG Cuts at: 2276 2276 Size: 6712 SacI G_AGCT'C Cuts at: 3773 3773 Size: 6712 SacII CC_GC'GG Cuts at: 2903 2903 Size: 6712 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SexAI A'CCwGG_T Cuts at: 2637 2637 Size: 6712 SfcI C'TryA_G Cuts at: 826 1045 2127 3087 4060 5195 5876 6067 826 Size: 219 1082 960 973 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 973 960 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 4039 1872 Size: 2167 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 4816 5717 5979 820 Size: 197 926 624 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 624 262 197 SnaBI TAC'GTA Cuts at: 493 4503 493 Size: 4010 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 4070 1132 Size: 2938 3774 SspI AAT'ATT Cuts at: 5 52 4636 5 Size: 47 4584 2081 Fragments arranged by size: 4584 2081 47 StuI AGG'CCT Cuts at: 1517 2519 1517 Size: 1002 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2664 3019 3924 4151 513 Size: 552 939 660 355 905 227 3074 Fragments arranged by size: 3074 939 905 660 552 355 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2401 2858 3575 4739 4756 4909 5094 Size: 1255 821 457 717 1164 17 153 185 Cuts at: 5094 5539 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1164 821 717 457 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 4005 4958 Size: 274 80 33 51 1443 348 1680 953 Cuts at: 4958 6670 96 Size: 1712 138 Fragments arranged by size: 1712 1680 1443 953 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3210 3240 3242 3272 6672 6704 3210 Size: 30 2 30 3400 32 3218 Fragments arranged by size: 3400 3218 32 30 30 2 Tth111I GACn'n_nGTC Cuts at: 1316 3869 1316 Size: 2553 4159 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3359 3359 Size: 6712 XhoI C'TCGA_G Cuts at: 1943 2567 1943 Size: 624 6088 XmaI C'CCGG_G Cuts at: 1870 4037 1870 Size: 2167 4545 XmnI GAAnn'nnTTC Cuts at: 3490 4841 3490 Size: 1351 5361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SanDI SapI SfiI SfoI SrfI SwaI