################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NdeI NotI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SmlI SnaBI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1249 GACGTC GACGT^C AccI 781 GTAGAC GT^[AC][GT]AC AflIII 1667 ACATGT A^C[GA][CT]GT AgeI 946 ACCGGT A^CCGGT AocI 101 CCTTAGG CC^T[ACGT]AGG Aor51HI 1537 AGCGCT AGC^GCT Asp700I 189 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 852 GGATCC G^GATCC BbeI 294 GGCGCC GGCGC^C BbsI 1135 GTCTTC GAAGAC(2/6) BcgI 1458 CGATGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1135 GTCTTC GAAGAC(2/6) BsaAI 1185 CACGTA [CT]AC^GT[GA] BsaI 1579 GAGACC GGTCTC(1/5) BsaWI 946 ACCGGT [AT]^CCGG[AT] BsiEI 443 CGGCCG CG[GA][CT]^CG BspLU11I 1667 ACATGT A^CATGT BsrDI 1175 CATTGC GCAATG(2/0) BssSI 592 CTCGTG CACGAG(-5/-1) BstEII 276 GGTCACC G^GT[ACGT]ACC BstPI 276 GGTCACC G^GT[ACGT]ACC BstXI 1649 CCAGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 101 CCTTAGG CC^T[ACGT]AGG EagI 443 CGGCCG C^GGCCG EarI 1492 CTCTTC CTCTTC(1/4) EclXI 443 CGGCCG C^GGCCG Eco47III 1537 AGCGCT AGC^GCT Eco52I 443 CGGCCG C^GGCCG Eco81I 101 CCTTAGG CC^T[ACGT]AGG EcoO65I 276 GGTCACC G^GT[ACGT]ACC HincII 1129 GTCAAC GT[CT]^[GA]AC HindII 1129 GTCAAC GT[CT]^[GA]AC KasI 294 GGCGCC G^GCGCC Ksp632I 1492 CTCTTC CTCTTC(1/4) NarI 294 GGCGCC GG^CGCC NcoI 1281 CCATGG C^CATGG NheI 1534 GCTAGC G^CTAGC NruI 1197 TCGCGA TCG^CGA PflMI 16 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 946 ACCGGT A^CCGGT PshAI 338 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 377 ACCTGGT A^CC[AT]GGT SfoI 294 GGCGCC GGC^GCC SgrAI 945 CACCGGTG C[GA]^CCGG[CT]G SpeI 339 ACTAGT A^CTAGT SphI 410 GCATGC GCATG^C StuI 812 AGGCCT AGG^CCT TfiI 726 GATTC G^A[AT]TC Van91I 16 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 189 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 942 GTGCAC G^TGCAC 1057 GTGCAC AlwNI 17 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 1385 CAGACCCTG ApaLI 942 GTGCAC G^TGCAC 1057 GTGCAC BfaI 340 CTAG C^TAG 1535 CTAG BglI 326 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1652 GCCTTGTGGGC BpmI 1105 CTGGAG CTGGAG(16/14) 1426 CTGGAG BseRI 120 CTCCTC GAGGAG(10/8) 955 GAGGAG BsmAI 1579 GAGAC GTCTC(1/5) 249 GTCTC BspMI 1190 ACCTGC ACCTGC(4/8) 1508 ACCTGC BsrBI 438 CCGCTC CCGCTC(-3/-3) 718 GAGCGG BstYI 800 GGATCT [GA]^GATC[CT] 852 GGATCC MaeI 340 CTAG C^TAG 1535 CTAG MflI 800 GGATCT [GA]^GATC[CT] 852 GGATCC MseI 665 TTAA T^TAA 923 TTAA MslI 786 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 996 CATATCAGTG MspA1I 1144 CAGCGG C[AC]G^C[GT]G 1551 CAGCGG NspI 410 GCATGC [GA]CATG^[CT] 1667 ACATGT SacI 502 GAGCTC GAGCT^C 958 GAGCTC Tru9I 665 TTAA T^TAA 923 TTAA Tsp509I 158 AATT ^AATT 921 AATT XhoII 800 GGATCT [GA]^GATC[CT] 852 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI