(Circular) (Six-base) MAPSORT of: pFN21AB7883.seq Check: 5034 from: 1 to: 6442 pFN21AB7883 6442 bp With 182 enzymes: SgfI * December 12, 2011 16:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3258 4252 278 Size: 53 83 186 936 1722 994 2468 Fragments arranged by size: 2468 1722 994 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 3785 1937 Size: 850 998 4594 Fragments arranged by size: 4594 998 850 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2789 3753 4505 5889 6157 1632 Size: 1157 964 752 1384 268 1917 Fragments arranged by size: 1917 1384 1157 964 752 268 AfeI AGC'GCT Cuts at: 3544 3544 Size: 6442 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 3672 5341 6318 1203 Size: 2469 1669 977 1327 Fragments arranged by size: 2469 1669 1327 977 AgeI A'CCGG_T Cuts at: 2951 5273 2951 Size: 2322 4120 AhdI GACnn_n'nnGTC Cuts at: 5171 5171 Size: 6442 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2510 1184 Size: 1326 5116 AlwNI CAG_nnn'CTG Cuts at: 2027 3395 4185 5757 2027 Size: 1368 790 1572 2712 Fragments arranged by size: 2712 1572 1368 790 ApaI G_GGCC'C Cuts at: 2228 2294 2366 2372 3635 2228 Size: 66 72 6 1263 5035 Fragments arranged by size: 5035 1263 72 66 6 ApaLI G'TGCA_C Cuts at: 2947 3062 4499 5655 2947 Size: 115 1437 1156 3734 Fragments arranged by size: 3734 1437 1156 115 ApoI r'AATT_y Cuts at: 1493 3751 3977 4129 6238 1493 Size: 2258 226 152 2109 1697 Fragments arranged by size: 2258 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 2227 2365 3628 3635 3724 3767 1870 Size: 73 284 138 1263 7 89 43 4545 Fragments arranged by size: 4545 1263 284 138 89 73 43 7 BamHI G'GATC_C Cuts at: 2857 3772 4139 2857 Size: 915 367 5160 Fragments arranged by size: 5160 915 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2299 3410 3526 3563 3647 Size: 325 217 627 512 1111 116 37 84 Cuts at: 3647 3763 5218 618 Size: 116 1455 1842 Fragments arranged by size: 1842 1455 1111 627 512 325 217 116 116 84 37 BanII G_rGCy'C Cuts at: 1391 2228 2294 2366 2372 2511 2877 2967 3126 Size: 837 66 72 6 139 366 90 159 Cuts at: 3126 3438 3635 3761 1391 Size: 312 197 126 4072 Fragments arranged by size: 4072 837 366 312 197 159 139 126 90 72 66 6 BbeI G_GCGC'C Cuts at: 2303 2303 Size: 6442 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3133 928 Size: 971 1234 4237 Fragments arranged by size: 4237 1234 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3452 3486 4633 4667 1903 Size: 34 50 34 1431 34 1147 34 3678 Fragments arranged by size: 3678 1431 1147 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4334 5544 4334 Size: 1210 5232 BclI T'GATC_A Cuts at: 1548 1548 Size: 6442 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2337 3663 5053 Size: 107 122 71 631 605 665 1326 1390 Cuts at: 5053 136 Size: 1525 Fragments arranged by size: 1525 1390 1326 665 631 605 122 107 71 BglII A'GATC_T Cuts at: 6437 6437 Size: 6442 BlpI GC'TnA_GC Cuts at: 3859 3859 Size: 6442 Bme1580I G_kGCm'C Cuts at: 1790 2228 2294 2366 2372 2951 3066 3635 4503 Size: 438 66 72 6 579 115 569 868 Cuts at: 4503 5659 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 868 579 569 438 115 72 66 6 BmgBI CAC'GTC Cuts at: 1318 2650 3061 1318 Size: 1332 411 4699 Fragments arranged by size: 4699 1332 411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2747 4173 5131 Size: 416 53 83 399 24 1330 1426 958 Cuts at: 5131 442 Size: 1753 Fragments arranged by size: 1753 1426 1330 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3543 1056 Size: 2487 3955 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 6442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3131 3452 5102 1239 Size: 1892 321 1650 2579 Fragments arranged by size: 2579 1892 1650 321 Bpu10I CC'TnA_GC Cuts at: 2273 3355 2273 Size: 1082 5360 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4567 5432 5730 4567 Size: 865 298 5279 Fragments arranged by size: 5279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3578 5105 882 Size: 617 2079 1527 2219 Fragments arranged by size: 2219 2079 1527 617 BsaAI yAC'GTr Cuts at: 493 1204 3192 4233 493 Size: 711 1988 1041 2702 Fragments arranged by size: 2702 1988 1041 711 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2300 3092 3255 4249 Size: 53 83 186 936 767 792 163 994 Cuts at: 4249 4631 5266 275 Size: 382 635 1451 Fragments arranged by size: 1451 994 936 792 767 635 382 186 163 83 53 BsaWI w'CCGG_w Cuts at: 2951 4875 5273 5547 5694 6105 2951 Size: 1924 398 274 147 411 3288 Fragments arranged by size: 3288 1924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6217 Fragments arranged by size: 6217 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 2975 1186 Size: 930 859 4653 Fragments arranged by size: 4653 930 859 BseYI C'CCAG_C Cuts at: 2098 2305 2353 2759 5645 6141 2098 Size: 207 48 406 2886 496 2399 Fragments arranged by size: 2886 2399 496 406 207 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2596 3008 3574 1163 Size: 573 860 412 566 4031 Fragments arranged by size: 4031 860 573 566 412 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2451 3892 4653 5681 1540 Size: 275 186 450 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 450 275 186 BsiHKAI G_wGCw'C Cuts at: 2156 2279 2511 2951 2967 3066 4503 4588 5659 Size: 123 232 440 16 99 1437 85 1071 Cuts at: 5659 2156 Size: 2939 Fragments arranged by size: 2939 1437 1071 440 232 123 99 85 16 BsmI GAATG_Cn' Cuts at: 3953 4046 3953 Size: 93 6349 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2228 2279 2294 2366 2372 2511 Size: 399 366 72 51 15 72 6 139 Cuts at: 2511 2877 2951 2967 3066 3126 3438 3635 3761 Size: 366 74 16 99 60 312 197 126 Cuts at: 3761 4503 4588 5659 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 366 312 197 139 126 99 85 74 72 72 60 51 16 15 6 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 4331 6061 4331 Size: 1730 4712 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3204 3522 3797 844 Size: 2360 318 275 3489 Fragments arranged by size: 3489 2360 318 275 BsrBI CCG'CTC Cuts at: 1394 1823 2445 2725 4329 1394 Size: 429 622 280 1604 3507 Fragments arranged by size: 3507 1604 622 429 280 BsrDI GCAATG_nn' Cuts at: 65 1248 3179 4937 5111 65 Size: 1183 1931 1758 174 1396 Fragments arranged by size: 1931 1758 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 2450 2951 3319 5086 5273 1700 Size: 110 273 367 501 368 1767 187 2869 Fragments arranged by size: 2869 1767 501 368 367 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 2597 4502 5514 2597 Size: 1905 1012 3525 Fragments arranged by size: 3525 1905 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 2281 1184 Size: 1097 5345 BstXI CCAn_nnnn'nTGG Cuts at: 3661 3661 Size: 6442 BstYI r'GATC_y Cuts at: 1972 2805 2857 3772 4139 4524 4541 5993 6079 Size: 833 52 915 367 385 17 1452 86 Cuts at: 6079 6091 6437 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 915 833 385 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 2107 2107 Size: 6442 BtgI C'CryG_G Cuts at: 513 1065 3286 3349 3573 3738 513 Size: 552 2221 63 224 165 3217 Fragments arranged by size: 3217 2221 552 224 165 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 3958 4752 4772 3958 Size: 794 20 5628 Fragments arranged by size: 5628 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 3746 3746 Size: 6442 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2448 3321 Size: 54 1425 50 275 18 217 401 873 Cuts at: 3321 3889 4778 6407 8 Size: 568 889 1629 43 Fragments arranged by size: 1629 1425 889 873 568 401 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2448 3889 1537 Size: 275 636 1441 4090 Fragments arranged by size: 4090 1441 636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3503 4372 1353 Size: 2150 869 3423 Fragments arranged by size: 3423 2150 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3322 5029 5403 5549 387 Size: 1064 138 1733 1707 374 146 1280 Fragments arranged by size: 1733 1707 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2509 2965 2509 Size: 456 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2789 3131 3452 3753 4505 5102 5889 Size: 393 1157 342 321 301 752 597 787 Cuts at: 5889 6157 1239 Size: 268 1524 Fragments arranged by size: 1524 1157 787 752 597 393 342 321 301 268 EcoO109I rG'GnC_Cy Cuts at: 2103 2112 2291 2763 3021 3106 3625 3632 3728 Size: 9 179 472 258 85 519 7 96 Cuts at: 3728 2103 Size: 4817 Fragments arranged by size: 4817 519 472 258 179 96 85 9 7 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 2303 2360 3546 5589 1678 Size: 625 57 1186 2043 2531 Fragments arranged by size: 2531 2043 1186 625 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2876 2908 2987 Size: 32 135 32 539 32 733 32 79 Cuts at: 2987 3019 3389 3421 5083 5115 5157 5189 1373 Size: 32 370 32 1662 32 42 32 2626 Fragments arranged by size: 2626 1662 733 539 370 135 79 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3136 3786 4032 669 Size: 988 281 1198 650 246 3079 Fragments arranged by size: 3079 1198 988 650 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KasI G'GCGC_C Cuts at: 2299 2299 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5556 5740 1305 Size: 4251 184 2007 Fragments arranged by size: 4251 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4620 Fragments arranged by size: 4620 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2795 3005 4400 4759 4918 518 Size: 648 1629 210 1395 359 159 2042 Fragments arranged by size: 2042 1629 1395 648 359 210 159 MspA1I CmG'CkG Cuts at: 1606 3151 3558 3858 4535 5683 5928 1606 Size: 1545 407 300 677 1148 245 2120 Fragments arranged by size: 2120 1545 1148 677 407 300 245 NaeI GCC'GGC Cuts at: 2085 2452 3321 2085 Size: 367 869 5206 Fragments arranged by size: 5206 869 367 NarI GG'CG_CC Cuts at: 2300 2300 Size: 6442 NcoI C'CATG_G Cuts at: 513 1065 3286 513 Size: 552 2221 3669 Fragments arranged by size: 3669 2221 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6442 NgoMIV G'CCGG_C Cuts at: 2083 2450 3319 2083 Size: 367 869 5206 Fragments arranged by size: 5206 869 367 NheI G'CTAG_C Cuts at: 1052 3539 1052 Size: 2487 3955 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NruI TCG'CGA Cuts at: 3204 3204 Size: 6442 NspI r_CATG'y Cuts at: 1132 1229 2419 3676 3800 5345 1132 Size: 97 1190 1257 124 1545 2229 Fragments arranged by size: 2229 1545 1257 1190 124 97 PasI CC'CwG_GG Cuts at: 1348 2287 2335 1348 Size: 939 48 5455 Fragments arranged by size: 5455 939 48 PciI A'CATG_T Cuts at: 3672 5341 3672 Size: 1669 4773 PflMI CCAn_nnn'nTGG Cuts at: 1108 2027 1108 Size: 919 5523 PfoI T'CCnGG_A Cuts at: 2147 2511 3327 3456 2147 Size: 364 816 129 5133 Fragments arranged by size: 5133 816 364 129 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4511 4543 6024 6056 4511 Size: 32 1481 32 4897 Fragments arranged by size: 4897 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2112 2763 3106 2112 Size: 651 343 5448 Fragments arranged by size: 5448 651 343 PshAI GACnn'nnGTC Cuts at: 1613 2347 1613 Size: 734 5708 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PspOMI G'GGCC_C Cuts at: 2224 2290 2362 2368 3631 2224 Size: 66 72 6 1263 5035 Fragments arranged by size: 5035 1263 72 66 6 PstI C_TGCA'G Cuts at: 830 2630 3150 3173 3794 830 Size: 1800 520 23 621 3478 Fragments arranged by size: 3478 1800 621 520 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 SacI G_AGCT'C Cuts at: 2511 2967 2511 Size: 456 5986 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SanDI GG'GwC_CC Cuts at: 2112 2763 2112 Size: 651 5791 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SexAI A'CCwGG_T Cuts at: 2382 2382 Size: 6442 SfcI C'TryA_G Cuts at: 826 1045 2626 3146 3169 3790 4925 5606 5797 Size: 219 1581 520 23 621 1135 681 191 Cuts at: 5797 826 Size: 1471 Fragments arranged by size: 1581 1471 1135 681 621 520 219 191 23 SfoI GGC'GCC Cuts at: 2301 2301 Size: 6442 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 2951 1700 Size: 1251 5191 SmaI CCC'GGG Cuts at: 1872 2229 2367 3630 3637 3726 3769 1872 Size: 357 138 1263 7 89 43 4545 Fragments arranged by size: 4545 1263 357 138 89 43 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 4546 5447 5709 820 Size: 197 926 2603 901 262 1553 Fragments arranged by size: 2603 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 2344 152 Size: 2192 4250 SphI G_CATG'C Cuts at: 1132 2419 3800 1132 Size: 1287 1381 3774 Fragments arranged by size: 3774 1381 1287 SrfI GCCC'GGGC Cuts at: 2229 2367 2229 Size: 138 6304 SspI AAT'ATT Cuts at: 5 52 4366 5 Size: 47 4314 2081 Fragments arranged by size: 4314 2081 47 StuI AGG'CCT Cuts at: 1517 2819 1517 Size: 1302 5140 StyI C'CwwG_G Cuts at: 513 1065 2321 2461 3259 3286 3881 513 Size: 552 1256 140 798 27 595 3074 Fragments arranged by size: 3074 1256 798 595 552 140 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2001 4469 4486 4639 4824 5269 325 Size: 1255 421 2468 17 153 185 445 1498 Fragments arranged by size: 2468 1498 1255 445 421 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 4688 6400 Size: 274 80 33 51 1443 204 2507 1712 Cuts at: 6400 96 Size: 138 Fragments arranged by size: 2507 1712 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6402 6434 6402 Size: 32 6410 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6442 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 2227 2365 3628 3635 3724 3767 1870 Size: 357 138 1263 7 89 43 4545 Fragments arranged by size: 4545 1263 357 138 89 43 7 XmnI GAAnn'nnTTC Cuts at: 2198 4571 2198 Size: 2373 4069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3256 4250 276 Size: 53 83 186 936 1722 994 2468 Fragments arranged by size: 2468 1722 994 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SfiI SwaI XcmI