(Linear) (Six-base) MAPSORT of: KIBB7883.seq Check: 2713 from: 1 to: 1737 KIBB7883.orf With 182 enzymes: SgfI * December 12, 2011 16:30 .. AatII G_ACGT'C Cuts at: 0 1253 1737 Size: 1253 484 AccI GT'mk_AC Cuts at: 0 782 1737 Size: 782 955 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 1737 Size: 784 953 AfeI AGC'GCT Cuts at: 0 1539 1737 Size: 1539 198 AflIII A'CryG_T Cuts at: 0 1667 1737 Size: 1667 70 AgeI A'CCGG_T Cuts at: 0 946 1737 Size: 946 791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 505 1737 Size: 505 1232 AlwNI CAG_nnn'CTG Cuts at: 0 22 1390 1737 Size: 22 1368 347 Fragments arranged by size: 1368 347 22 ApaI G_GGCC'C Cuts at: 0 223 289 361 367 1630 1737 Size: 223 66 72 6 1263 107 Fragments arranged by size: 1263 223 107 72 66 6 ApaLI G'TGCA_C Cuts at: 0 942 1057 1737 Size: 942 115 680 Fragments arranged by size: 942 680 115 AvaI C'yCGr_G Cuts at: 0 222 360 1623 1630 1719 1737 Size: 222 138 1263 7 89 18 Fragments arranged by size: 1263 222 138 89 18 7 BamHI G'GATC_C Cuts at: 0 852 1737 Size: 852 885 BanI G'GyrC_C Cuts at: 0 294 1405 1521 1558 1642 1737 Size: 294 1111 116 37 84 95 Fragments arranged by size: 1111 294 116 95 84 37 BanII G_rGCy'C Cuts at: 0 223 289 361 367 506 872 962 1121 Size: 223 66 72 6 139 366 90 159 Cuts at: 1121 1433 1630 1737 Size: 312 197 107 Fragments arranged by size: 366 312 223 197 159 139 107 90 72 66 6 BbeI G_GCGC'C Cuts at: 0 298 1737 Size: 298 1439 BbsI GAAGACnn'nnnn_ Cuts at: 0 1128 1737 Size: 1128 609 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1447 1481 1737 Size: 1447 34 256 Fragments arranged by size: 1447 256 34 BglI GCCn_nnn'nGGC Cuts at: 0 332 1658 1737 Size: 332 1326 79 Fragments arranged by size: 1326 332 79 Bme1580I G_kGCm'C Cuts at: 0 223 289 361 367 946 1061 1630 1737 Size: 223 66 72 6 579 115 569 107 Fragments arranged by size: 579 569 223 115 107 72 66 6 BmgBI CAC'GTC Cuts at: 0 645 1056 1737 Size: 645 411 681 Fragments arranged by size: 681 645 411 BmrI ACTGGGnnnn_n' Cuts at: 0 742 1737 Size: 742 995 BmtI G_CTAG'C Cuts at: 0 1538 1737 Size: 1538 199 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 456 1737 Size: 456 1281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1126 1447 1737 Size: 1126 321 290 Fragments arranged by size: 1126 321 290 Bpu10I CC'TnA_GC Cuts at: 0 268 1350 1737 Size: 268 1082 387 Fragments arranged by size: 1082 387 268 BsaI GGTCTCn'nnnn_ Cuts at: 0 1573 1737 Size: 1573 164 BsaAI yAC'GTr Cuts at: 0 1187 1737 Size: 1187 550 BsaHI Gr'CG_yC Cuts at: 0 295 1087 1250 1737 Size: 295 792 163 487 Fragments arranged by size: 792 487 295 163 BsaWI w'CCGG_w Cuts at: 0 946 1737 Size: 946 791 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 111 970 1737 Size: 111 859 767 Fragments arranged by size: 859 767 111 BseYI C'CCAG_C Cuts at: 0 93 300 348 754 1737 Size: 93 207 48 406 983 Fragments arranged by size: 983 406 207 93 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 591 1003 1569 1737 Size: 591 412 566 168 Fragments arranged by size: 591 566 412 168 BsiEI CG_ry'CG Cuts at: 0 446 1737 Size: 446 1291 BsiHKAI G_wGCw'C Cuts at: 0 151 274 506 946 962 1061 1737 Size: 151 123 232 440 16 99 676 Fragments arranged by size: 676 440 232 151 123 99 16 Bsp1286I G_dGCh'C Cuts at: 0 151 223 274 289 361 367 506 872 Size: 151 72 51 15 72 6 139 366 Cuts at: 872 946 962 1061 1121 1433 1630 1737 Size: 74 16 99 60 312 197 107 Fragments arranged by size: 366 312 197 151 139 107 99 74 72 72 60 51 16 15 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1199 1517 1737 Size: 1199 318 220 Fragments arranged by size: 1199 318 220 BsrBI CCG'CTC Cuts at: 0 440 720 1737 Size: 440 280 1017 Fragments arranged by size: 1017 440 280 BsrDI GCAATG_nn' Cuts at: 0 1174 1737 Size: 1174 563 BsrFI r'CCGG_y Cuts at: 0 78 445 946 1314 1737 Size: 78 367 501 368 423 Fragments arranged by size: 501 423 368 367 78 BssSI C'ACGA_G Cuts at: 0 592 1737 Size: 592 1145 BstEII G'GTnAC_C Cuts at: 0 276 1737 Size: 276 1461 BstXI CCAn_nnnn'nTGG Cuts at: 0 1656 1737 Size: 1656 81 BstYI r'GATC_y Cuts at: 0 800 852 1737 Size: 800 52 885 Fragments arranged by size: 885 800 52 Bsu36I CC'TnA_GG Cuts at: 0 102 1737 Size: 102 1635 BtgI C'CryG_G Cuts at: 0 1281 1344 1568 1737 Size: 1281 63 224 169 Fragments arranged by size: 1281 224 169 63 EaeI y'GGCC_r Cuts at: 0 42 443 1316 1737 Size: 42 401 873 421 Fragments arranged by size: 873 421 401 42 EagI C'GGCC_G Cuts at: 0 443 1737 Size: 443 1294 EarI CTCTTCn'nnn_ Cuts at: 0 1498 1737 Size: 1498 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1317 1737 Size: 1317 420 EcoICRI GAG'CTC Cuts at: 0 504 960 1737 Size: 504 456 777 Fragments arranged by size: 777 504 456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 1126 1447 1737 Size: 784 342 321 290 Fragments arranged by size: 784 342 321 290 EcoO109I rG'GnC_Cy Cuts at: 0 98 107 286 758 1016 1101 1620 1627 Size: 98 9 179 472 258 85 519 7 Cuts at: 1627 1723 1737 Size: 96 14 Fragments arranged by size: 519 472 258 179 98 96 85 14 9 7 HaeII r_GCGC'y Cuts at: 0 298 355 1541 1737 Size: 298 57 1186 196 Fragments arranged by size: 1186 298 196 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 871 903 982 1014 1384 1416 Size: 106 32 733 32 79 32 370 32 Cuts at: 1416 1737 Size: 321 Fragments arranged by size: 733 370 321 106 79 32 32 32 32 HincII GTy'rAC Cuts at: 0 1131 1737 Size: 1131 606 KasI G'GCGC_C Cuts at: 0 294 1737 Size: 294 1443 MslI CAynn'nnrTG Cuts at: 0 790 1000 1737 Size: 790 210 737 Fragments arranged by size: 790 737 210 MspA1I CmG'CkG Cuts at: 0 1146 1553 1737 Size: 1146 407 184 Fragments arranged by size: 1146 407 184 NaeI GCC'GGC Cuts at: 0 80 447 1316 1737 Size: 80 367 869 421 Fragments arranged by size: 869 421 367 80 NarI GG'CG_CC Cuts at: 0 295 1737 Size: 295 1442 NcoI C'CATG_G Cuts at: 0 1281 1737 Size: 1281 456 NgoMIV G'CCGG_C Cuts at: 0 78 445 1314 1737 Size: 78 367 869 423 Fragments arranged by size: 869 423 367 78 NheI G'CTAG_C Cuts at: 0 1534 1737 Size: 1534 203 NruI TCG'CGA Cuts at: 0 1199 1737 Size: 1199 538 NspI r_CATG'y Cuts at: 0 414 1671 1737 Size: 414 1257 66 Fragments arranged by size: 1257 414 66 PasI CC'CwG_GG Cuts at: 0 282 330 1737 Size: 282 48 1407 Fragments arranged by size: 1407 282 48 PciI A'CATG_T Cuts at: 0 1667 1737 Size: 1667 70 PflMI CCAn_nnn'nTGG Cuts at: 0 22 1737 Size: 22 1715 PfoI T'CCnGG_A Cuts at: 0 142 506 1322 1451 1737 Size: 142 364 816 129 286 Fragments arranged by size: 816 364 286 142 129 PpuMI rG'GwC_Cy Cuts at: 0 107 758 1101 1737 Size: 107 651 343 636 Fragments arranged by size: 651 636 343 107 PshAI GACnn'nnGTC Cuts at: 0 342 1737 Size: 342 1395 PspOMI G'GGCC_C Cuts at: 0 219 285 357 363 1626 1737 Size: 219 66 72 6 1263 111 Fragments arranged by size: 1263 219 111 72 66 6 PstI C_TGCA'G Cuts at: 0 625 1145 1168 1737 Size: 625 520 23 569 Fragments arranged by size: 625 569 520 23 SacI G_AGCT'C Cuts at: 0 506 962 1737 Size: 506 456 775 Fragments arranged by size: 775 506 456 SanDI GG'GwC_CC Cuts at: 0 107 758 1737 Size: 107 651 979 Fragments arranged by size: 979 651 107 SexAI A'CCwGG_T Cuts at: 0 377 1737 Size: 377 1360 SfcI C'TryA_G Cuts at: 0 621 1141 1164 1737 Size: 621 520 23 573 Fragments arranged by size: 621 573 520 23 SfoI GGC'GCC Cuts at: 0 296 1737 Size: 296 1441 SgrAI Cr'CCGG_yG Cuts at: 0 946 1737 Size: 946 791 SmaI CCC'GGG Cuts at: 0 224 362 1625 1632 1721 1737 Size: 224 138 1263 7 89 16 Fragments arranged by size: 1263 224 138 89 16 7 SpeI A'CTAG_T Cuts at: 0 339 1737 Size: 339 1398 SphI G_CATG'C Cuts at: 0 414 1737 Size: 414 1323 SrfI GCCC'GGGC Cuts at: 0 224 362 1737 Size: 224 138 1375 Fragments arranged by size: 1375 224 138 StuI AGG'CCT Cuts at: 0 814 1737 Size: 814 923 StyI C'CwwG_G Cuts at: 0 316 456 1254 1281 1737 Size: 316 140 798 27 456 Fragments arranged by size: 798 456 316 140 27 TatI w'GTAC_w Cuts at: 0 176 1737 Size: 176 1561 XmaI C'CCGG_G Cuts at: 0 222 360 1623 1630 1719 1737 Size: 222 138 1263 7 89 18 Fragments arranged by size: 1263 222 138 89 18 7 XmnI GAAnn'nnTTC Cuts at: 0 193 1737 Size: 193 1544 ZraI GAC'GTC Cuts at: 0 1251 1737 Size: 1251 486 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AgeI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI SacI SanDI SexAI SfcI SfoI SgrAI SmaI SpeI SphI SrfI StuI StyI TatI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII BlpI BpuEI BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NotI NsiI PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfiI SgfI SmlI SnaBI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI