Restriction map for
pFN21AE1224
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AocI 10 CCTCAGG CC^T[ACGT]AGG
ApaI 546 GGGCCC GGGCC^C
AvaI 345 CCCGGG C^[CT]CG[GA]G
BsaAI 489 CACGTA [CT]AC^GT[GA]
BsmBI 653 GAGACG CGTCTC(1/5)
BsoBI 345 CCCGGG C^[CT]CG[GA]G
BsrBI 643 CCGCTC CCGCTC(-3/-3)
BsrI 226 CCAGT ACTGG(1/-1)
BstAPI 713 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 159 GGTCACC G^GT[ACGT]ACC
BstF5I 483 CATCC GGATG(2/0)
BstPI 159 GGTCACC G^GT[ACGT]ACC
Bsu36I 10 CCTCAGG CC^T[ACGT]AGG
CfoI 612 GCGC GCG^C
DrdI 220 GACACCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 73 TGGCCG [CT]^GGCC[GA]
Eco81I 10 CCTCAGG CC^T[ACGT]AGG
EcoNI 29 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 159 GGTCACC G^GT[ACGT]ACC
FauI 642 CCCGC CCCGC(4/6)
FokI 483 CATCC GGATG(9/13)
HaeII 611 GGCGCT [GA]GCGC^[CT]
HgaI 655 GACGC GACGC(5/10)
HhaI 612 GCGC GCG^C
HinP1I 612 GCGC G^CGC
MboII 661 GAAGA GAAGA(8/7)
NlaIII 146 CATG CATG^
NspI 145 GCATGC [GA]CATG^[CT]
PflMI 725 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 535 GATGC GCATC(5/9)
SmaI 345 CCCGGG CCC^GGG
SphI 145 GCATGC GCATG^C
TaqI 431 TCGA T^CGA
TthHB8I 431 TCGA T^CGA
Van91I 725 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 725 CCAGCTGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 345 CCCGGG C^CCGGG
XmaI 345 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 389 AGCT AG^CT
727 AGCT
AlwI 314 GGATC GGATC(4/5)
692 GGATC
AlwNI 533 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
726 CAGCTGCTG
BanII 546 GGGCCC G[GA]GC[CT]^C
604 GAGCCC
BglI 320 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
759 GCCCAAGAGGC
BsmAI 184 GAGAC GTCTC(1/5)
653 GAGAC
BsrFI 51 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
Cfr10I 51 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
EcoT14I 34 CCAAGG C^C[AT][AT]GG
296 CCAAGG
MaeII 105 ACGT A^CGT
490 ACGT
MspA1I 388 CAGCTG C[AC]G^C[GT]G
726 CAGCTG
NaeI 51 GCCGGC GCC^GGC
75 GCCGGC
NgoMIV 51 GCCGGC G^CCGGC
75 GCCGGC
PvuII 388 CAGCTG CAG^CTG
726 CAGCTG
StyI 34 CCAAGG C^C[AT][AT]GG
296 CCAAGG
TfiI 292 GATTC G^A[AT]TC
523 GAATC
TspRI 114 CACTG CA[GC]TG[ACGT][ACGT]^
188 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AvaII BanI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TseI Tsp45I