################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 827 GGTACC G^GTACC AcsI 85 GAATTC [GA]^AATT[CT] AgeI 416 ACCGGT A^CCGGT ApoI 85 GAATTC [GA]^AATT[CT] Asp700I 909 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 827 GGTACC G^GTACC BalI 425 TGGCCA TGG^CCA BglI 470 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 950 GTGCCC G[AGT]GC[ACT]^C BsaWI 416 ACCGGT [AT]^CCGG[AT] Bsp1286I 950 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 884 TGTACA T^GTACA BspHI 458 TCATGA T^CATGA BsrGI 884 TGTACA T^GTACA BsrI 522 CCAGT ACTGG(1/-1) BstEII 894 GGTCACC G^GT[ACGT]ACC BstPI 894 GGTCACC G^GT[ACGT]ACC BstXI 152 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 959 GCAGGC GC[ACGT]^[ACGT]GC DrdI 348 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 281 CCACGG C^C[GA][CT]GG EaeI 425 TGGCCA [CT]^GGCC[GA] EarI 861 GAAGAG CTCTTC(1/4) EcoNI 682 CCTATCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 894 GGTCACC G^GT[ACGT]ACC EcoRI 85 GAATTC G^AATTC EcoT14I 254 CCTTGG C^C[AT][AT]GG KpnI 827 GGTACC GGTAC^C Ksp632I 861 GAAGAG CTCTTC(1/4) MluNI 425 TGGCCA TGG^CCA MscI 425 TGGCCA TGG^CCA MseI 484 TTAA T^TAA MspA1I 839 CAGCTG C[AC]G^C[GT]G MwoI 470 GCCTCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PinAI 416 ACCGGT A^CCGGT PstI 121 CTGCAG CTGCA^G PvuII 839 CAGCTG CAG^CTG RcaI 458 TCATGA T^CATGA SexAI 158 ACCTGGT A^CC[AT]GGT SspBI 884 TGTACA T^GTACA StyI 254 CCTTGG C^C[AT][AT]GG Tru9I 484 TTAA T^TAA XmnI 909 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AocI 612 CCTTAGG CC^T[ACGT]AGG 927 CCTGAGG AvaII 129 GGTCC G^G[AT]CC 285 GGTCC BamHI 111 GGATCC G^GATCC 692 GGATCC BanI 661 GGTGCC G^G[CT][GA]CC 827 GGTACC BseRI 91 CTCCTC GAGGAG(10/8) 604 CTCCTC BsgI 442 CTGCAC GTGCAG(16/14) 957 GTGCAG BsmAI 79 GTCTC GTCTC(1/5) 677 GTCTC BsmFI 99 GGGAC GGGAC(10/14) 286 GTCCC BspMI 179 ACCTGC ACCTGC(4/8) 553 ACCTGC BsrFI 126 GCCGGT [GA]^CCGG[CT] 416 ACCGGT BstYI 111 GGATCC [GA]^GATC[CT] 692 GGATCC Bsu36I 612 CCTTAGG CC^T[ACGT]AGG 927 CCTGAGG Cfr10I 126 GCCGGT [GA]^CCGG[CT] 416 ACCGGT Cfr13I 129 GGTCC G^G[ACGT]CC 285 GGTCC DpnII 112 GATC ^GATC 693 GATC Eco81I 612 CCTTAGG CC^T[ACGT]AGG 927 CCTGAGG HaeIII 408 GGCC GG^CC 426 GGCC HapII 127 CCGG C^CGG 417 CCGG HpaII 127 CCGG C^CGG 417 CCGG MboI 112 GATC ^GATC 693 GATC MflI 111 GGATCC [GA]^GATC[CT] 692 GGATCC MslI 222 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 897 CACCCCCATG MspI 127 CCGG C^CGG 417 CCGG NdeII 112 GATC ^GATC 693 GATC Sau3AI 112 GATC ^GATC 693 GATC Sau96I 129 GGTCC G^G[ACGT]CC 285 GGTCC SfcI 121 CTGCAG C^T[GA][CT]AG 921 CTACAG TspRI 878 CAGTG CA[GC]TG[ACGT][ACGT]^ 888 CACTG XhoII 111 GGATCC [GA]^GATC[CT] 692 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AluI AlwI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HinfI HphI ItaI MaeIII MboII MnlI MvaI NlaIII NlaIV NspI PleI RsaI ScrFI TfiI TseI Tsp45I Tsp509I XcmI