(Circular) (Six-base) MAPSORT of: pFN21AE5486.seq Check: 4193 from: 1 to: 5722 pFN21AE5486 5722 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2567 2567 Size: 5722 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 2832 3043 2832 Size: 211 5511 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2865 3785 5169 5437 1632 Size: 1233 920 1384 268 1917 Fragments arranged by size: 1917 1384 1233 920 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 2421 4553 2421 Size: 2132 3590 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2818 1184 Size: 1634 4088 AlwNI CAG_nnn'CTG Cuts at: 3465 5037 3465 Size: 1572 4150 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2090 3031 3257 3409 5518 1493 Size: 597 941 226 152 2109 1697 Fragments arranged by size: 2109 1697 941 597 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 3047 1870 Size: 73 1104 4545 Fragments arranged by size: 4545 1104 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2824 2857 2824 Size: 33 5689 BamHI G'GATC_C Cuts at: 2116 2697 3052 3419 2116 Size: 581 355 367 4419 Fragments arranged by size: 4419 581 367 355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2666 2832 3043 4498 618 Size: 325 217 627 879 166 211 1455 1842 Fragments arranged by size: 1842 1455 879 627 325 217 211 166 BanII G_rGCy'C Cuts at: 1391 3041 1391 Size: 1650 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4751 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2481 4333 136 Size: 107 122 71 631 605 809 1852 1525 Fragments arranged by size: 1852 1525 809 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2959 3783 4939 1790 Size: 1169 824 1156 2573 Fragments arranged by size: 2573 1169 1156 824 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2521 3453 4411 Size: 416 53 83 399 24 1104 932 958 Cuts at: 4411 442 Size: 1753 Fragments arranged by size: 1753 1104 958 932 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3847 4712 5010 3847 Size: 865 298 4559 Fragments arranged by size: 4559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2421 4155 4553 4827 4974 5385 2421 Size: 1734 398 274 147 411 2758 Fragments arranged by size: 2758 1734 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5497 Fragments arranged by size: 5497 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2600 1186 Size: 901 513 4308 Fragments arranged by size: 4308 901 513 BseYI C'CCAG_C Cuts at: 2141 2176 4925 5421 2141 Size: 35 2749 496 2442 Fragments arranged by size: 2749 2442 496 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 2983 1163 Size: 573 696 551 3902 Fragments arranged by size: 3902 696 573 551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3783 3868 4939 3783 Size: 85 1071 4566 Fragments arranged by size: 4566 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2959 3041 3783 3868 4939 1391 Size: 399 1169 82 742 85 1071 2174 Fragments arranged by size: 2174 1169 1071 742 399 85 82 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 2463 3611 5341 2463 Size: 1148 1730 2844 Fragments arranged by size: 2844 1730 1148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 2567 3077 844 Size: 1349 374 510 3489 Fragments arranged by size: 3489 1349 510 374 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2131 2421 4366 4553 1700 Size: 110 321 290 1945 187 2869 Fragments arranged by size: 2869 1945 321 290 187 110 BsrGI T'GTAC_A Cuts at: 96 2889 96 Size: 2793 2929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 2899 1184 Size: 1715 4007 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2164 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2116 2697 3052 3419 3804 3821 5273 5359 Size: 144 581 355 367 385 17 1452 86 Cuts at: 5359 5371 5717 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 581 385 367 355 346 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2618 2933 2618 Size: 315 5407 BtgI C'CryG_G Cuts at: 513 1065 2286 513 Size: 552 1221 3949 Fragments arranged by size: 3949 1221 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 3238 4032 4052 3238 Size: 794 20 4908 Fragments arranged by size: 4908 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2359 4729 809 Size: 507 1043 2370 1802 Fragments arranged by size: 2370 1802 1043 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 3169 4058 Size: 54 1425 50 275 18 600 739 889 Cuts at: 4058 5687 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 739 600 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2861 3652 1353 Size: 1508 791 3423 Fragments arranged by size: 3423 1508 791 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2865 3785 4382 5169 5437 1239 Size: 393 1233 920 597 787 268 1524 Fragments arranged by size: 1524 1233 920 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2691 2691 Size: 5722 EcoRI G'AATT_C Cuts at: 2090 3031 3409 2090 Size: 941 378 4403 Fragments arranged by size: 4403 941 378 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4363 4395 4437 4469 1373 Size: 32 135 32 2791 32 42 32 2626 Fragments arranged by size: 2791 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3066 3312 669 Size: 988 281 1128 246 3079 Fragments arranged by size: 3079 1128 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 2836 3047 2836 Size: 211 5511 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 2652 2768 4836 5020 1305 Size: 969 378 116 2068 184 2007 Fragments arranged by size: 2068 2007 969 378 184 116 MscI TGG'CCA Cuts at: 10 64 1489 1832 2432 10 Size: 54 1425 343 600 3300 Fragments arranged by size: 3300 1425 600 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2906 3680 4039 4198 518 Size: 648 1065 675 774 359 159 2042 Fragments arranged by size: 2042 1065 774 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2846 3138 3815 4963 5208 1606 Size: 1240 292 677 1148 245 2120 Fragments arranged by size: 2120 1240 1148 677 292 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5170 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2314 2385 2398 3080 4625 1132 Size: 97 1085 71 13 682 1545 2229 Fragments arranged by size: 2229 1545 1085 682 97 71 13 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 2130 3074 830 Size: 1300 944 3478 Fragments arranged by size: 3478 1300 944 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2846 2846 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SexAI A'CCwGG_T Cuts at: 2163 2163 Size: 5722 SfcI C'TryA_G Cuts at: 826 1045 2126 2926 3070 4205 4886 5077 826 Size: 219 1081 800 144 1135 681 191 1471 Fragments arranged by size: 1471 1135 1081 800 681 219 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3826 4727 4989 820 Size: 197 926 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 2259 3161 513 Size: 552 1194 902 3074 Fragments arranged by size: 3074 1194 902 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2654 3749 3766 3919 4104 4549 325 Size: 1255 1074 1095 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1095 1074 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 2889 3968 Size: 274 80 33 51 1443 258 654 1079 Cuts at: 3968 5680 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1079 654 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2302 2869 2257 Size: 45 567 5110 Fragments arranged by size: 5110 567 45 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 2918 3851 2918 Size: 933 4789 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI