Restriction map for
pFN21AE0800
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AclI AcyI AflII
Alw44I Aor51HI ApaI ApaLI AscI
AspHI AspI AviII BalI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspHI BspMI BsrBI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I CfoI
ClaI CpoI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I KasI KspI
MluI MluNI MscI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 2371 GGTACC G^GTACC
AccII 3641 CGCG CG^CG
AccIII 330 TCCGGA T^CCGGA
AgeI 4288 ACCGGT A^CCGGT
AhdI 474 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2041 CCTGAGG CC^T[ACGT]AGG
Asp718I 2371 GGTACC G^GTACC
AspEI 474 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4523 CTCGGG C^[CT]CG[GA]G
AvrII 3111 CCTAGG C^CTAGG
BciVI 1904 GTATCC GTATCC(6/5)
BglII 2137 AGATCT A^GATCT
BlnI 3111 CCTAGG C^CTAGG
BlpI 2427 GCTCAGC GC^T[ACGT]AGC
BmyI 1848 GGGCAC G[AGT]GC[ACT]^C
BpmI 1708 CTGGAG CTGGAG(16/14)
Bpu1102I 2427 GCTCAGC GC^T[ACGT]AGC
BsaAI 3013 CACGTA [CT]AC^GT[GA]
BsaBI 3247 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 330 TCCGGA T^CCGGA
BsmI 3116 GAATGC GAATGC(1/-1)
BsoBI 4523 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1848 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 242 TGTACA T^GTACA
BspEI 330 TCCGGA T^CCGGA
BsrFI 4288 ACCGGT [GA]^CCGG[CT]
BsrGI 242 TGTACA T^GTACA
BssSI 3935 CACGAG CACGAG(-5/-1)
BstBI 4446 TTCGAA TT^CGAA
BstUI 3641 CGCG CG^CG
Bsu36I 2041 CCTGAGG CC^T[ACGT]AGG
CelII 2427 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4288 ACCGGT [GA]^CCGG[CT]
DraII 293 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1356 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 474 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2041 CCTGAGG CC^T[ACGT]AGG
EcoO109I 293 GGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 1587 GACGC GACGC(5/10)
HindIII 1484 AAGCTT A^AGCTT
HpaI 1992 GTTAAC GTT^AAC
KpnI 2371 GGTACC GGTAC^C
MamI 3247 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 330 TCCGGA T^CCGGA
MvnI 3641 CGCG CG^CG
NcoI 2537 CCATGG C^CATGG
NspV 4446 TTCGAA TT^CGAA
PacI 3217 TTAATTAA TTAAT^TAA
PinAI 4288 ACCGGT A^CCGGT
PpuMI 293 GGGACCC [GA]G^G[AT]CC[CT]
PstI 3432 CTGCAG CTGCA^G
SfuI 4446 TTCGAA TT^CGAA
SpeI 2977 ACTAGT A^CTAGT
SspBI 242 TGTACA T^GTACA
XbaI 2817 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 2249 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3356 CAGATTCTG
AseI 1660 ATTAAT AT^TAAT
3216 ATTAAT
AsnI 1660 ATTAAT AT^TAAT
3216 ATTAAT
Asp700I 561 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2809 GAACAAGTTC
BclI 990 TGATCA T^GATCA
2466 TGATCA
BsaWI 330 TCCGGA [AT]^CCGG[AT]
4288 ACCGGT
BseRI 2852 CTCCTC GAGGAG(10/8)
4520 CTCCTC
BsrDI 14 CATTGC GCAATG(2/0)
948 CATTGC
BstXI 1394 CCAAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2235 CCAATTAGCTGG
DraI 676 TTTAAA TTT^AAA
3024 TTTAAA
DsaI 2537 CCATGG C^C[GA][CT]GG
3949 CCACGG
EcoRI 1310 GAATTC G^AATTC
4341 GAATTC
FbaI 990 TGATCA T^GATCA
2466 TGATCA
HapII 331 CCGG C^CGG
4289 CCGG
HpaII 331 CCGG C^CGG
4289 CCGG
MaeII 2473 ACGT A^CGT
3014 ACGT
MslI 2272 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3672 CATAGAAATG
MspA1I 1283 CAGCTG C[AC]G^C[GT]G
4511 CAGCTG
MspI 331 CCGG C^CGG
4289 CCGG
NdeI 728 CATATG CA^TATG
4495 CATATG
PshBI 1660 ATTAAT AT^TAAT
3216 ATTAAT
PvuII 1283 CAGCTG CAG^CTG
4511 CAGCTG
SapI 596 GAAGAGC GCTCTTC(1/4)
722 GAAGAGC
SexAI 2169 ACCAGGT A^CC[AT]GGT
3807 ACCAGGT
SmlI 616 CTCAAG C^T[CT][GA]AG
3743 CTCAAG
XmnI 561 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2809 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BamHI BanI
BbsI BbvI BfaI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BspLU11I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII