################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AvrII BalI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII Eco47III EcoRI EcoRV FbaI FseI HgaI HpaI KpnI KspI MamI MluI MluNI MroI MscI NaeI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 1347 GTCTAC GT^[AC][GT]AC AccII 55 CGCG CG^CG AcsI 152 AAATTC [GA]^AATT[CT] AcyI 1631 GGCGCC G[GA]^CG[CT]C AfaI 769 GTAC GT^AC AflIII 1568 ACACGT A^C[GA][CT]GT AocI 1948 CCTAAGG CC^T[ACGT]AGG ApoI 152 AAATTC [GA]^AATT[CT] AviII 605 TGCGCA TGC^GCA BbeI 1631 GGCGCC GGCGC^C BfaI 655 CTAG C^TAG BsaHI 1631 GGCGCC G[GA]^CG[CT]C BsgI 807 CTGCAC GTGCAG(16/14) BsiEI 1447 CGGCCG CG[GA][CT]^CG BsrBI 1638 CCGCTC CCGCTC(-3/-3) BssSI 1847 CACGAG CACGAG(-5/-1) BstUI 55 CGCG CG^CG Bsu36I 1948 CCTAAGG CC^T[ACGT]AGG EagI 1447 CGGCCG C^GGCCG EclXI 1447 CGGCCG C^GGCCG Eco52I 1447 CGGCCG C^GGCCG Eco81I 1948 CCTAAGG CC^T[ACGT]AGG EcoNI 1101 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 589 ATGCAT ATGCA^T FspI 605 TGCGCA TGC^GCA HaeII 1631 GGCGCC [GA]GCGC^[CT] Hin1I 1631 GGCGCC G[GA]^CG[CT]C HincII 543 GTCAAC GT[CT]^[GA]AC HindII 543 GTCAAC GT[CT]^[GA]AC HindIII 1942 AAGCTT A^AGCTT KasI 1631 GGCGCC G^GCGCC MaeI 655 CTAG C^TAG MfeI 728 CAATTG C^AATTG MseI 374 TTAA T^TAA MslI 420 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 728 CAATTG C^AATTG MvnI 55 CGCG CG^CG NarI 1631 GGCGCC GG^CGCC NcoI 419 CCATGG C^CATGG NsiI 589 ATGCAT ATGCA^T PaeR7I 236 CTCGAG C^TCGAG PstI 1102 CTGCAG CTGCA^G PvuII 185 CAGCTG CAG^CTG RsaI 769 GTAC GT^AC SapI 531 GCTCTTC GCTCTTC(1/4) SbfI 1101 CCTGCAGG CCTGCA^GG SexAI 902 ACCTGGT A^CC[AT]GGT SfoI 1631 GGCGCC GGC^GCC SmaI 275 CCCGGG CCC^GGG Sse8387I 1101 CCTGCAGG CCTGCA^GG StuI 1686 AGGCCT AGG^CCT Tru9I 374 TTAA T^TAA XhoI 236 CTCGAG C^TCGAG XmaCI 275 CCCGGG C^CCGGG XmaI 275 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AhdI 38 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1311 GACAGGCGGTC ApaI 1702 GGGCCC GGGCC^C 1953 GGGCCC AspEI 38 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1311 GACAGGCGGTC BamHI 1413 GGATCC G^GATCC 1536 GGATCC BsaI 802 GAGACC GGTCTC(1/5) 1811 GAGACC BsrDI 632 GCAATG GCAATG(2/0) 1064 GCAATG BstEII 414 GGTTACC G^GT[ACGT]ACC 777 GGTGACC BstPI 414 GGTTACC G^GT[ACGT]ACC 777 GGTGACC BstXI 785 CCATTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 896 CCAGGAACCTGG DrdI 1666 GACAGGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1742 GACCTCAACGTC Eam1105I 38 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1311 GACAGGCGGTC EcoO65I 414 GGTTACC G^GT[ACGT]ACC 777 GGTGACC EcoT14I 419 CCATGG C^C[AT][AT]GG 1985 CCTTGG MspA1I 185 CAGCTG C[AC]G^C[GT]G 1200 CAGCGG PpuMI 710 AGGACCT [GA]G^G[AT]CC[CT] 1470 GGGACCC SmlI 236 CTCGAG C^T[CT][GA]AG 865 CTTGAG StyI 419 CCATGG C^C[AT][AT]GG 1985 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI SacI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII