Restriction map for
pFN21AE2491
(Circular) (Six-base) MAPSORT of: pFN21AE2491.seq Check: 524 from: 1 to: 11764
pFN21AE2491 11764 bp
With 182 enzymes: SgfI *
January 8, 2014 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5653 5653
Size: 11764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4206 5108 9574 278
Size: 53 83 186 936 2670 902 4466 2468
Fragments arranged by size:
4466 2670 2468 936 902 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3558 6561 7518 9107 1937
Size: 1621 3003 957 1589 4594
Fragments arranged by size:
4594 3003 1621 1589 957
Acc65I G'GTAC_C
Cuts at: 9085 9085
Size: 11764
AclI AA'CG_TT
Cuts at: 1563 9891 10264 1563
Size: 8328 373 3063
Fragments arranged by size:
8328 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4115 4333 4372 4550 4700 4774 4853
Size: 1775 708 218 39 178 150 74 79
Cuts at: 4853 5183 7022 7202 7211 7360 8877 9827 11211
Size: 330 1839 180 9 149 1517 950 1384
Cuts at: 11211 11479 1632
Size: 268 1917
Fragments arranged by size:
1917 1839 1775 1517 1384 950 708 330
268 218 180 178 150 149 79 74
39 9
AfeI AGC'GCT
Cuts at: 2399 8753 2399
Size: 6354 5410
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11567
AflIII A'CryG_T
Cuts at: 1203 2511 3039 6648 7800 10663 11640 1203
Size: 1308 528 3609 1152 2863 977 1327
Fragments arranged by size:
3609 2863 1327 1308 1152 977 528
AgeI A'CCGG_T
Cuts at: 5847 10595 5847
Size: 4748 7016
AhdI GACnn_n'nnGTC
Cuts at: 3712 5104 10493 3712
Size: 1392 5389 4983
Fragments arranged by size:
5389 4983 1392
AleI CACnn'nnGTG
Cuts at: 1166 3072 6977 7229 1166
Size: 1906 3905 252 5701
Fragments arranged by size:
5701 3905 1906 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5033 1184
Size: 3849 7915
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 4862 4894 2852
Size: 32 1978 32 9722
Fragments arranged by size:
9722 1978 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3716 3794 4611 4915 5754 6484 8123 9507 11079
Size: 78 817 304 839 730 1639 1384 1572
Cuts at: 11079 3716
Size: 4401
Fragments arranged by size:
4401 1639 1572 1384 839 817 730 304
78
ApaI G_GGCC'C
Cuts at: 3772 5405 5568 5824 6244 6389 7667 8522 3772
Size: 1633 163 256 420 145 1278 855 7014
Fragments arranged by size:
7014 1633 1278 855 420 256 163 145
ApaLI G'TGCA_C
Cuts at: 3545 3719 5239 6844 7674 7907 8060 9821 10977
Size: 174 1520 1605 830 233 153 1761 1156
Cuts at: 10977 3545
Size: 4332
Fragments arranged by size:
4332 1761 1605 1520 1156 830 233 174
153
ApoI r'AATT_y
Cuts at: 1493 4190 9073 9299 9451 11560 1493
Size: 2697 4883 226 152 2109 1697
Fragments arranged by size:
4883 2697 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2052 3666 2052
Size: 1614 10150
AseI AT'TA_AT
Cuts at: 160 1032 10318 160
Size: 872 9286 1606
Fragments arranged by size:
9286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11764
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2693 3049 3503 3626 3771 4309
Size: 73 159 591 356 454 123 145 538
Cuts at: 4309 4457 5561 6244 7667 8004 8822 9089 1870
Size: 148 1104 683 1423 337 818 267 4545
Fragments arranged by size:
4545 1423 1104 818 683 591 538 454
356 337 267 159 148 145 123 73
AvrII C'CTAG_G
Cuts at: 8096 8096
Size: 11764
BamHI G'GATC_C
Cuts at: 2655 8110 9094 9461 2655
Size: 5455 984 367 4958
Fragments arranged by size:
5455 4958 984 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2107 2192 2280 3074
Size: 325 217 627 254 66 85 88 794
Cuts at: 3074 3507 3635 3830 3929 4710 5196 5411 5799
Size: 433 128 195 99 781 486 215 388
Cuts at: 5799 6167 6741 7724 7816 7828 7880 8720 9085
Size: 368 574 983 92 12 52 840 365
Cuts at: 9085 10540 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 983 840 794 781 627 574
486 433 388 368 365 325 254 217
215 195 128 99 92 88 85 66
52 12
BanII G_rGCy'C
Cuts at: 1391 2102 2142 2165 3224 3772 4146 5276 5405
Size: 711 40 23 1059 548 374 1130 129
Cuts at: 5405 5568 5784 5824 6244 6389 7667 7895 7986
Size: 163 216 40 420 145 1278 228 91
Cuts at: 7986 8165 8355 8522 8535 8570 8612 8706 8717
Size: 179 190 167 13 35 42 94 11
Cuts at: 8717 9083 1391
Size: 366 4072
Fragments arranged by size:
4072 1278 1130 1059 711 548 420 374
366 228 216 190 179 167 163 145
129 94 91 42 40 40 35 23
13 11
BbeI G_GCGC'C
Cuts at: 2111 3639 4714 5415 6171 6745 7728 7832 8724
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 8724 2111
Size: 5151
Fragments arranged by size:
5151 1528 1075 983 892 756 701 574
104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2233 2716 4739 4841 5902 5974 6961
Size: 971 334 483 2023 102 1061 72 987
Cuts at: 6961 6979 7344 928
Size: 18 365 5348
Fragments arranged by size:
5348 2023 1061 987 971 483 365 334
102 72 18
BbvCI CC'TCA_GC
Cuts at: 5541 5987 9022 5541
Size: 446 3035 8283
Fragments arranged by size:
8283 3035 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8594 8628 9955 9989 1903
Size: 34 6657 34 1327 34 3678
Fragments arranged by size:
6657 3678 1327 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2671 9656 10866 2671
Size: 6985 1210 3569
Fragments arranged by size:
6985 3569 1210
BclI T'GATC_A
Cuts at: 1548 3126 3234 5893 1548
Size: 1578 108 2659 7419
Fragments arranged by size:
7419 2659 1578 108
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2231 3525 3776
Size: 107 122 71 631 605 559 1294 251
Cuts at: 3776 4554 5409 5617 10375 136
Size: 778 855 208 4758 1525
Fragments arranged by size:
4758 1525 1294 855 778 631 605 559
251 208 122 107 71
BglII A'GATC_T
Cuts at: 11759 11759
Size: 11764
BlpI GC'TnA_GC
Cuts at: 4145 4427 8778 9181 4145
Size: 282 4351 403 6728
Fragments arranged by size:
6728 4351 403 282
Bme1580I G_kGCm'C
Cuts at: 1790 2046 3319 3510 3549 3723 3772 3833 3942
Size: 256 1273 191 39 174 49 61 109
Cuts at: 3942 4050 4079 4123 4294 5199 5243 5395 5405
Size: 108 29 44 171 905 44 152 10
Cuts at: 5405 5458 5568 5824 6244 6389 6848 7667 7678
Size: 53 110 256 420 145 459 819 11
Cuts at: 7678 7911 8012 8064 8522 8910 9825 10981 1790
Size: 233 101 52 458 388 915 1156 2573
Fragments arranged by size:
2573 1273 1156 915 905 819 459 458
420 388 256 256 233 191 174 171
152 145 110 109 108 101 61 53
52 49 44 44 39 29 11 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3474 4019 5357
Size: 416 53 83 399 24 2057 545 1338
Cuts at: 5357 5400 5788 7540 8019 8826 8986 9495 10453
Size: 43 388 1752 479 807 160 509 958
Cuts at: 10453 442
Size: 1753
Fragments arranged by size:
2057 1753 1752 1338 958 807 545 509
479 416 399 388 160 83 53 43
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11764
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 11764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 4145 4499 4538 5132 5222 5416 5437
Size: 1193 1713 354 39 594 90 194 21
Cuts at: 5437 5458 5798 5963 7118 7346 7613 7670 7925
Size: 21 340 165 1155 228 267 57 255
Cuts at: 7925 8227 8720 9023 10424 1239
Size: 302 493 303 1401 2579
Fragments arranged by size:
2579 1713 1401 1193 1155 594 493 354
340 303 302 267 255 228 194 165
90 57 39 21 21
Bpu10I CC'TnA_GC
Cuts at: 5541 5987 6284 7358 7490 8424 8716 9022 5541
Size: 446 297 1074 132 934 292 306 8283
Fragments arranged by size:
8283 1074 934 446 306 297 292 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5450 6236 6935 9889 10754 11052 5450
Size: 786 699 2954 865 298 6162
Fragments arranged by size:
6162 2954 865 786 699 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5346 7859 8832 10427 882
Size: 617 3847 2513 973 1595 2219
Fragments arranged by size:
3847 2513 2219 1595 973 617
BsaAI yAC'GTr
Cuts at: 493 1204 2317 2514 3394 5899 9555 493
Size: 711 1113 197 880 2505 3656 2702
Fragments arranged by size:
3656 2702 2505 1113 880 711 197
BsaBI GATnn'nnATC
Cuts at: 2459 3113 4409 11758 2459
Size: 654 1296 7349 2465
Fragments arranged by size:
7349 2465 1296 654
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 2108 2424 2748
Size: 53 83 186 936 554 21 316 324
Cuts at: 2748 3171 3636 4182 4203 4711 5105 5412 6168
Size: 423 465 546 21 508 394 307 756
Cuts at: 6168 6660 6742 7635 7725 7829 8721 9571 9953
Size: 492 82 893 90 104 892 850 382
Cuts at: 9953 10588 275
Size: 635 1451
Fragments arranged by size:
1451 936 893 892 850 756 635 554
546 508 492 465 423 394 382 324
316 307 186 104 90 83 82 53
21 21
BsaWI w'CCGG_w
Cuts at: 4563 4844 5847 8374 10197 10595 10869 11016 11427
Size: 281 1003 2527 1823 398 274 147 411
Cuts at: 11427 4563
Size: 4900
Fragments arranged by size:
4900 2527 1823 1003 411 398 281 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 2549 2579 2852
Size: 30 165 30 714 30 402 30 273
Cuts at: 2852 2882 4839 4862 4869 4892 5819 5849 6596
Size: 30 1957 23 7 23 927 30 747
Cuts at: 6596 6626 8225 8255 8678 8708 1178
Size: 30 1599 30 423 30 4234
Fragments arranged by size:
4234 1957 1599 927 747 714 423 402
273 165 30 30 30 30 30 30
30 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2864 2869 2873 4475 4957 5135 6722
Size: 1030 648 5 4 1602 482 178 1587
Cuts at: 6722 6740 6766 7115 7610 8285 8315 1186
Size: 18 26 349 495 675 30 4635
Fragments arranged by size:
4635 1602 1587 1030 675 648 495 482
349 178 30 26 18 5 4
BseYI C'CCAG_C
Cuts at: 2188 2958 3141 3826 4061 4069 4221 4281 5297
Size: 770 183 685 235 8 152 60 1016
Cuts at: 5297 5459 5678 6441 6773 6786 7300 7966 8290
Size: 162 219 763 332 13 514 666 324
Cuts at: 8290 8482 8754 8795 10967 11463 2188
Size: 192 272 41 2172 496 2489
Fragments arranged by size:
2489 2172 1016 770 763 685 666 514
496 332 324 272 235 219 192 183
162 152 60 41 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2932 2985 4096 4744 6371 6596 6981
Size: 573 1196 53 1111 648 1627 225 385
Cuts at: 6981 7351 9069 1163
Size: 370 1718 3858
Fragments arranged by size:
3858 1718 1627 1196 1111 648 573 385
370 225 53
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2172 2270 2834 2926 5621 5625
Size: 275 186 171 98 564 92 2695 4
Cuts at: 5625 5653 8252 9214 9975 11003 1540
Size: 28 2599 962 761 1028 2301
Fragments arranged by size:
2695 2599 2301 1028 962 761 564 275
186 171 98 92 28 4
BsiHKAI G_wGCw'C
Cuts at: 2142 2316 2912 3549 3723 4146 5022 5243 5276
Size: 174 596 637 174 423 876 221 33
Cuts at: 5276 6075 6645 6767 6848 6918 7469 7581 7678
Size: 799 570 122 81 70 551 112 97
Cuts at: 7678 7911 8064 8784 9825 9910 10981 2142
Size: 233 153 720 1041 85 1071 2925
Fragments arranged by size:
2925 1071 1041 876 799 720 637 596
570 551 423 233 221 174 174 153
122 112 97 85 81 70 33
BsmI GAATG_Cn'
Cuts at: 9275 9368 9275
Size: 93 11671
BsmBI CGTCTCn'nnnn_
Cuts at: 3269 5863 5998 6260 8245 3269
Size: 2594 135 262 1985 6788
Fragments arranged by size:
6788 2594 1985 262 135
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2102 2142 2165 2316 2912 3224
Size: 399 256 56 40 23 151 596 312
Cuts at: 3224 3319 3510 3549 3723 3772 3833 3942 4050
Size: 95 191 39 174 49 61 109 108
Cuts at: 4050 4079 4123 4146 4294 5022 5199 5243 5276
Size: 29 44 23 148 728 177 44 33
Cuts at: 5276 5395 5405 5458 5568 5784 5824 6075 6244
Size: 119 10 53 110 216 40 251 169
Cuts at: 6244 6389 6645 6767 6848 6918 7469 7581 7667
Size: 145 256 122 81 70 551 112 86
Cuts at: 7667 7678 7895 7911 7986 8012 8064 8165 8355
Size: 11 217 16 75 26 52 101 190
Cuts at: 8355 8522 8535 8570 8612 8706 8717 8784 8910
Size: 167 13 35 42 94 11 67 126
Cuts at: 8910 9083 9825 9910 10981 1391
Size: 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 728 596 551 399 312
256 256 251 217 216 191 190 177
174 173 169 167 151 148 145 126
122 119 112 110 109 108 101 95
94 86 85 81 75 70 67 61
56 53 52 49 44 44 42 40
40 39 35 33 29 26 23 23
16 13 11 11 10
BspEI T'CCGG_A
Cuts at: 4563 11427 4563
Size: 6864 4900
BspHI T'CATG_A
Cuts at: 3231 4401 4998 7443 7581 9653 11383 3231
Size: 1170 597 2445 138 2072 1730 3612
Fragments arranged by size:
3612 2445 2072 1730 1170 597 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5037 5653 6090 6108 7506 8406 9119 844
Size: 4193 616 437 18 1398 900 713 3489
Fragments arranged by size:
4193 3489 1398 900 713 616 437 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 3048 5977 6828 7977 8019 8392
Size: 429 305 920 2929 851 1149 42 373
Cuts at: 8392 8537 9651 1394
Size: 145 1114 3507
Fragments arranged by size:
3507 2929 1149 1114 920 851 429 373
305 145 42
BsrDI GCAATG_nn'
Cuts at: 65 1248 7167 10259 10433 65
Size: 1183 5919 3092 174 1396
Fragments arranged by size:
5919 3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3914 5424 5620 5847 6687 6792 7501
Size: 110 2104 1510 196 227 840 105 709
Cuts at: 7501 7877 8034 8146 8247 8251 8381 10408 10595
Size: 376 157 112 101 4 130 2027 187
Cuts at: 10595 1700
Size: 2869
Fragments arranged by size:
2869 2104 2027 1510 840 709 376 227
196 187 157 130 112 110 105 101
4
BsrGI T'GTAC_A
Cuts at: 96 4944 96
Size: 4848 6916
BssHII G'CGCG_C
Cuts at: 1925 2052 3666 7210 8546 1925
Size: 127 1614 3544 1336 5143
Fragments arranged by size:
5143 3544 1614 1336 127
BssSI C'ACGA_G
Cuts at: 4718 5045 9824 10836 4718
Size: 327 4779 1012 5646
Fragments arranged by size:
5646 4779 1012 327
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2280 2946 3012 3794 5773 6574 794
Size: 1486 666 66 782 1979 801 5984
Fragments arranged by size:
5984 1979 1486 801 782 666 66
BstBI TT'CG_AA
Cuts at: 10568 10568
Size: 11764
BstEII G'GTnAC_C
Cuts at: 1184 4214 4342 8149 8948 1184
Size: 3030 128 3807 799 4000
Fragments arranged by size:
4000 3807 3030 799 128
BstXI CCAn_nnnn'nTGG
Cuts at: 3826 4022 5047 5941 6022 6178 6910 6937 3826
Size: 196 1025 894 81 156 732 27 8653
Fragments arranged by size:
8653 1025 894 732 196 156 81 27
BstYI r'GATC_y
Cuts at: 1972 2655 3981 4911 5081 6174 7944 8110 9094
Size: 683 1326 930 170 1093 1770 166 984
Cuts at: 9094 9461 9846 9863 11315 11401 11413 11759 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1770 1452 1326 1093 984 930 683
385 367 346 170 166 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3809 3859 8523 3809
Size: 50 4664 7050
Fragments arranged by size:
7050 4664 50
BtgI C'CryG_G
Cuts at: 513 1065 2235 2303 3601 3694 4177 4393 4737
Size: 552 1170 68 1298 93 483 216 344
Cuts at: 4737 5473 5606 6150 6552 6909 7419 7812 8189
Size: 736 133 544 402 357 510 393 377
Cuts at: 8189 8356 8625 9060 513
Size: 167 269 435 3217
Fragments arranged by size:
3217 1298 1170 736 552 544 510 483
435 402 393 377 357 344 269 216
167 133 93 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5085 5920 487
Size: 683 56 3859 835 6331
Fragments arranged by size:
6331 3859 835 683 56
BtsI GCAGTG_nn'
Cuts at: 3792 5579 7339 7483 8233 8468 9280 10074 10094
Size: 1787 1760 144 750 235 812 794 20
Cuts at: 10094 3792
Size: 5462
Fragments arranged by size:
5462 1787 1760 812 794 750 235 144
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11729
DraI TTT'AAA
Cuts at: 9068 9068
Size: 11764
DraIII CAC_nnn'GTG
Cuts at: 2581 4244 8866 2581
Size: 1663 4622 5479
Fragments arranged by size:
5479 4622 1663
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8303 8825 10771 809
Size: 507 6987 522 1946 1802
Fragments arranged by size:
6987 1946 1802 522 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2232 2267
Size: 54 1425 50 275 18 339 63 35
Cuts at: 2267 2831 2923 3345 3816 3993 4024 4174 4407
Size: 564 92 422 471 177 31 150 233
Cuts at: 4407 5037 5178 5367 5476 5618 5622 6012 6147
Size: 630 141 189 109 142 4 390 135
Cuts at: 6147 7062 7572 8249 8379 9211 10100 11729 8
Size: 915 510 677 130 832 889 1629 43
Fragments arranged by size:
1629 1425 915 889 832 677 630 564
510 471 422 390 339 275 233 189
177 150 142 141 135 130 109 92
63 54 50 43 35 31 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2169 2267 2831 2923 5618 5622 8249
Size: 275 357 98 564 92 2695 4 2627
Cuts at: 8249 9211 1537
Size: 962 4090
Fragments arranged by size:
4090 2695 2627 962 564 357 275 98
92 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3372 4091 4781 5027 5837 6362 6872 8896
Size: 2019 719 690 246 810 525 510 2024
Cuts at: 8896 8957 9694 1353
Size: 61 737 3423
Fragments arranged by size:
3423 2024 2019 810 737 719 690 525
510 246 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2138 4646 5078 5750 6041 6719
Size: 1064 138 549 2508 432 672 291 678
Cuts at: 6719 7905 8280 8314 8492 10351 10725 10871 387
Size: 1186 375 34 178 1859 374 146 1280
Fragments arranged by size:
2508 1859 1280 1186 1064 678 672 549
432 375 374 291 178 146 138 34
EcoICRI GAG'CTC
Cuts at: 2140 4144 5274 2140
Size: 2004 1130 8630
Fragments arranged by size:
8630 2004 1130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 3407 4115 4145 4333 4372 4499
Size: 393 800 975 708 30 188 39 127
Cuts at: 4499 4538 4550 4700 4774 4853 5132 5183 5222
Size: 39 12 150 74 79 279 51 39
Cuts at: 5222 5416 5437 5458 5798 5963 7022 7118 7202
Size: 194 21 21 340 165 1059 96 84
Cuts at: 7202 7211 7346 7360 7613 7670 7925 8227 8720
Size: 9 135 14 253 57 255 302 493
Cuts at: 8720 8877 9023 9827 10424 11211 11479 1239
Size: 157 146 804 597 787 268 1524
Fragments arranged by size:
1524 1059 975 804 800 787 708 597
493 393 340 302 279 268 255 253
194 188 165 157 150 146 135 127
96 84 79 74 57 51 39 39
39 30 21 21 14 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2803 3687 4919 5048 6476 9036 2803
Size: 884 1232 129 1428 2560 5531
Fragments arranged by size:
5531 2560 1428 1232 884 129
EcoO109I rG'GnC_Cy
Cuts at: 3500 3768 4018 4129 4225 5338 5401 5402 5565
Size: 268 250 111 96 1113 63 1 163
Cuts at: 5565 5821 6241 6385 6386 6481 6946 7354 7663
Size: 256 420 144 1 95 465 408 309
Cuts at: 7663 7664 8395 8518 8519 8661 8731 8827 9015
Size: 1 731 123 1 142 70 96 188
Cuts at: 9015 3500
Size: 6249
Fragments arranged by size:
6249 1113 731 465 420 408 309 268
256 250 188 163 144 142 123 111
96 96 95 70 63 1 1 1
1
EcoRI G'AATT_C
Cuts at: 4190 9073 9451 4190
Size: 4883 378 6503
Fragments arranged by size:
6503 4883 378
EcoRV GAT'ATC
Cuts at: 6276 6276
Size: 11764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6478 8239 6478
Size: 1761 10003
FseI GG_CCGG'CC
Cuts at: 5624 5624
Size: 11764
FspI TGC'GCA
Cuts at: 2939 4655 5696 8402 10270 2939
Size: 1716 1041 2706 1868 4433
Fragments arranged by size:
4433 2706 1868 1716 1041
HaeII r_GCGC'y
Cuts at: 1678 2111 2401 3000 3520 3639 4714 5415 5431
Size: 433 290 599 520 119 1075 701 16
Cuts at: 5431 5473 6171 6745 6793 7119 7728 7832 8724
Size: 42 698 574 48 326 609 104 892
Cuts at: 8724 8755 10911 1678
Size: 31 2156 2531
Fragments arranged by size:
2531 2156 1075 892 701 698 609 599
574 520 433 326 290 119 104 48
42 31 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 3161 3193 3698
Size: 32 135 32 545 32 1012 32 505
Cuts at: 3698 3730 4459 4491 5090 5122 5723 5755 5878
Size: 32 729 32 599 32 601 32 123
Cuts at: 5878 5910 8594 8626 10405 10437 10479 10511 1373
Size: 32 2684 32 1779 32 42 32 2626
Fragments arranged by size:
2684 2626 1779 1012 729 601 599 545
505 135 123 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4420 6562 6892 9108 9354 669
Size: 988 281 2482 2142 330 2216 246 3079
Fragments arranged by size:
3079 2482 2216 2142 988 330 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11764
HpaI GTT'AAC
Cuts at: 9354 9354
Size: 11764
KasI G'GCGC_C
Cuts at: 2107 3635 4710 5411 6167 6741 7724 7828 8720
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 8720 2107
Size: 5151
Fragments arranged by size:
5151 1528 1075 983 892 756 701 574
104
KpnI G_GTAC'C
Cuts at: 9089 9089
Size: 11764
MfeI C'AATT_G
Cuts at: 9363 9363
Size: 11764
MluI A'CGCG_T
Cuts at: 11640 11640
Size: 11764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2547 3084 3354 5109 10878 11062 1305
Size: 1242 537 270 1755 5769 184 2007
Fragments arranged by size:
5769 2007 1755 1242 537 270 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3347 3818 4026 4409 5039
Size: 54 1425 343 1515 471 208 383 630
Cuts at: 5039 6149 7064 10
Size: 1110 915 4710
Fragments arranged by size:
4710 1515 1425 1110 915 630 471 383
343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2540 3072 3215 4403 4436 4505
Size: 648 1299 75 532 143 1188 33 69
Cuts at: 4505 4595 5045 5255 6020 6143 6653 6902 6908
Size: 90 450 210 765 123 510 249 6
Cuts at: 6908 6977 7157 7229 8582 9722 10081 10240 518
Size: 69 180 72 1353 1140 359 159 2042
Fragments arranged by size:
2042 1353 1299 1188 1140 765 648 532
510 450 359 249 210 180 159 143
123 90 75 72 69 69 33 6
MspA1I CmG'CkG
Cuts at: 1606 2277 2781 3286 3298 3313 3369 3447 3459
Size: 671 504 505 12 15 56 78 12
Cuts at: 3459 3696 3800 3873 4073 4149 4221 4285 4353
Size: 237 104 73 200 76 72 64 68
Cuts at: 4353 5246 5436 5475 5751 6093 6441 7054 7846
Size: 893 190 39 276 342 348 613 792
Cuts at: 7846 8490 8627 8815 9180 9857 11005 11250 1606
Size: 644 137 188 365 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 792 677 671 644 613
505 504 365 348 342 276 245 237
200 190 188 137 104 78 76 73
72 68 64 56 39 15 12 12
NaeI GCC'GGC
Cuts at: 5426 5622 6689 6794 7503 7879 8036 8249 8253
Size: 196 1067 105 709 376 157 213 4
Cuts at: 8253 8383 5426
Size: 130 8807
Fragments arranged by size:
8807 1067 709 376 213 196 157 130
105 4
NarI GG'CG_CC
Cuts at: 2108 3636 4711 5412 6168 6742 7725 7829 8721
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 8721 2108
Size: 5151
Fragments arranged by size:
5151 1528 1075 983 892 756 701 574
104
NcoI C'CATG_G
Cuts at: 513 1065 2303 3601 4737 6150 6909 8356 513
Size: 552 1238 1298 1136 1413 759 1447 3921
Fragments arranged by size:
3921 1447 1413 1298 1238 1136 759 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11764
NgoMIV G'CCGG_C
Cuts at: 5424 5620 6687 6792 7501 7877 8034 8247 8251
Size: 196 1067 105 709 376 157 213 4
Cuts at: 8251 8381 5424
Size: 130 8807
Fragments arranged by size:
8807 1067 709 376 213 196 157 130
105 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11764
NotI GC'GGCC_GC
Cuts at: 2267 9211 2267
Size: 6944 4820
NspI r_CATG'y
Cuts at: 1132 1229 2326 2362 2653 2902 2944 3785 3992
Size: 97 1097 36 291 249 42 841 207
Cuts at: 3992 4510 4807 6652 7210 7804 9122 10667 1132
Size: 518 297 1845 558 594 1318 1545 2229
Fragments arranged by size:
2229 1845 1545 1318 1097 841 594 558
518 297 291 249 207 97 42 36
PasI CC'CwG_GG
Cuts at: 1348 2047 2353 2953 5397 6052 8155 9038 1348
Size: 699 306 600 2444 655 2103 883 4074
Fragments arranged by size:
4074 2444 2103 883 699 655 600 306
PciI A'CATG_T
Cuts at: 6648 7800 10663 6648
Size: 1152 2863 7749
Fragments arranged by size:
7749 2863 1152
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4720 9044 1108
Size: 3612 4324 3828
Fragments arranged by size:
4324 3828 3612
PfoI T'CCnGG_A
Cuts at: 2409 2787 5283 7769 8433 2409
Size: 378 2496 2486 664 5740
Fragments arranged by size:
5740 2496 2486 664 378
PmeI GTTT'AAAC
Cuts at: 9068 9068
Size: 11764
PmlI CAC'GTG
Cuts at: 2317 2514 5899 2317
Size: 197 3385 8182
Fragments arranged by size:
8182 3385 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 4862 4894 9833 9865 11346 11378 2852
Size: 32 1978 32 4939 32 1481 32 3238
Fragments arranged by size:
4939 3238 1978 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3500 4129 4225 5338 8395 8661 8731 8827 9015
Size: 629 96 1113 3057 266 70 96 188
Cuts at: 9015 3500
Size: 6249
Fragments arranged by size:
6249 3057 1113 629 266 188 96 96
70
PshAI GACnn'nnGTC
Cuts at: 1613 5108 1613
Size: 3495 8269
PsiI TTA'TAA
Cuts at: 9334 9334
Size: 11764
PspOMI G'GGCC_C
Cuts at: 3768 5401 5564 5820 6240 6385 7663 8518 3768
Size: 1633 163 256 420 145 1278 855 7014
Fragments arranged by size:
7014 1633 1278 855 420 256 163 145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3017 3049 6518 6550 3017
Size: 32 3469 32 8231
Fragments arranged by size:
8231 3469 32 32
PstI C_TGCA'G
Cuts at: 830 6087 7851 8048 8119 8172 8740 9116 830
Size: 5257 1764 197 71 53 568 376 3478
Fragments arranged by size:
5257 3478 1764 568 376 197 71 53
PvuI CG_AT'CG
Cuts at: 2001 5653 2001
Size: 3652 8112
PvuII CAG'CTG
Cuts at: 2277 3298 3369 5246 5436 5751 6093 7054 2277
Size: 1021 71 1877 190 315 342 961 6987
Fragments arranged by size:
6987 1877 1021 961 342 315 190 71
SacI G_AGCT'C
Cuts at: 2142 4146 5276 2142
Size: 2004 1130 8630
Fragments arranged by size:
8630 2004 1130
SacII CC_GC'GG
Cuts at: 3697 5476 8628 3697
Size: 1779 3152 6833
Fragments arranged by size:
6833 3152 1779
SalI G'TCGA_C
Cuts at: 1936 6560 9106 1936
Size: 4624 2546 4594
Fragments arranged by size:
4624 4594 2546
SanDI GG'GwC_CC
Cuts at: 3500 4225 5338 8731 8827 9015 3500
Size: 725 1113 3393 96 188 6249
Fragments arranged by size:
6249 3393 1113 725 188 96
SapI GCTCTTCn'nnn_
Cuts at: 3372 4091 4781 5027 6362 3372
Size: 719 690 246 1335 8774
Fragments arranged by size:
8774 1335 719 690 246
SbfI CC_TGCA'GG
Cuts at: 9116 9116
Size: 11764
ScaI AGT'ACT
Cuts at: 2561 3032 4385 6956 10012 11724 2561
Size: 471 1353 2571 3056 1712 2601
Fragments arranged by size:
3056 2601 2571 1712 1353 471
SexAI A'CCwGG_T
Cuts at: 3552 7743 3552
Size: 4191 7573
SfcI C'TryA_G
Cuts at: 826 1045 6083 7847 8044 8115 8168 8410 8736
Size: 219 5038 1764 197 71 53 242 326
Cuts at: 8736 9112 10247 10928 11119 826
Size: 376 1135 681 191 1471
Fragments arranged by size:
5038 1764 1471 1135 681 376 326 242
219 197 191 71 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2231 5617 2231
Size: 3386 8378
SfoI GGC'GCC
Cuts at: 2109 3637 4712 5413 6169 6743 7726 7830 8722
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 8722 2109
Size: 5151
Fragments arranged by size:
5151 1528 1075 983 892 756 701 574
104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11764
SmaI CCC'GGG
Cuts at: 1872 2104 3505 3773 4311 5563 6246 7669 9091
Size: 232 1401 268 538 1252 683 1423 1422
Cuts at: 9091 1872
Size: 4545
Fragments arranged by size:
4545 1423 1422 1401 1252 683 538 268
232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5465 6251 6950 9868 10769 11031
Size: 197 926 3522 786 699 2918 901 262
Cuts at: 11031 820
Size: 1553
Fragments arranged by size:
3522 2918 1553 926 901 786 699 262
197
SnaBI TAC'GTA
Cuts at: 493 9555 493
Size: 9062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11764
SphI G_CATG'C
Cuts at: 1132 2326 2653 2902 2944 4807 7210 9122 1132
Size: 1194 327 249 42 1863 2403 1912 3774
Fragments arranged by size:
3774 2403 1912 1863 1194 327 249 42
SrfI GCCC'GGGC
Cuts at: 5563 5563
Size: 11764
SspI AAT'ATT
Cuts at: 5 52 9688 5
Size: 47 9636 2081
Fragments arranged by size:
9636 2081 47
StuI AGG'CCT
Cuts at: 1517 2417 2966 5816 5950 7699 8282 8513 8765
Size: 900 549 2850 134 1749 583 231 252
Cuts at: 8765 1517
Size: 4516
Fragments arranged by size:
4516 2850 1749 900 583 549 252 231
134
StyI C'CwwG_G
Cuts at: 513 1065 2226 2303 2475 3601 3900 4737 6150
Size: 552 1161 77 172 1126 299 837 1413
Cuts at: 6150 6411 6510 6909 7887 8073 8096 8356 8742
Size: 261 99 399 978 186 23 260 386
Cuts at: 8742 9203 513
Size: 461 3074
Fragments arranged by size:
3074 1413 1161 1126 978 837 552 461
399 386 299 261 260 186 172 99
77 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2180 2243 2495 3133 5098 6170
Size: 1255 444 156 63 252 638 1965 1072
Cuts at: 6170 6335 6433 6511 6545 8531 9791 9808 9961
Size: 165 98 78 34 1986 1260 17 153
Cuts at: 9961 10146 10591 325
Size: 185 445 1498
Fragments arranged by size:
1986 1965 1498 1260 1255 1072 638 445
444 252 185 165 156 153 98 78
63 34 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2559 3030 3156
Size: 274 80 33 51 1443 582 471 126
Cuts at: 3156 4383 4944 6456 6603 6954 10010 11722 96
Size: 1227 561 1512 147 351 3056 1712 138
Fragments arranged by size:
3056 1712 1512 1443 1227 582 561 471
351 274 147 138 126 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 2565 2597 4839 4871 7982 8014 8054
Size: 32 164 32 2242 32 3111 32 40
Cuts at: 8054 8086 8095 8127 8225 8257 8678 8710 11724
Size: 32 9 32 98 32 421 32 3014
Cuts at: 11724 11756 2369
Size: 32 2377
Fragments arranged by size:
3111 3014 2377 2242 421 164 98 40
32 32 32 32 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 1316 2033 6140 8665 1316
Size: 717 4107 2525 4415
Fragments arranged by size:
4415 4107 2525 717
XbaI T'CTAG_A
Cuts at: 9100 9100
Size: 11764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2759 3599 3811 4744 5197 8908 2759
Size: 840 212 933 453 3711 5615
Fragments arranged by size:
5615 3711 933 840 453 212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11764
XmaI C'CCGG_G
Cuts at: 1870 2102 3503 3771 4309 5561 6244 7667 9089
Size: 232 1401 268 538 1252 683 1423 1422
Cuts at: 9089 1870
Size: 4545
Fragments arranged by size:
4545 1423 1422 1401 1252 683 538 268
232
XmnI GAAnn'nnTTC
Cuts at: 2867 4871 5849 9893 2867
Size: 2004 978 4044 4738
Fragments arranged by size:
4738 4044 2004 978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4204 5106 9572 276
Size: 53 83 186 936 2670 902 4466 2468
Fragments arranged by size:
4466 2670 2468 936 902 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
BaeI BfrBI BsiWI BstZ17I FspAI NruI NsiI PacI
PspXI RsrII SwaI