################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AflII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmI BsoBI BspDI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstUI BstXI CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 210 GACGCC G[GA]^CG[CT]C AflIII 1048 ACATGT A^C[GA][CT]GT Aor51HI 485 AGCGCT AGC^GCT ApaI 1040 GGGCCC GGGCC^C Asp700I 827 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1308 GAGCTC G[AT]GC[AT]^C BamHI 954 GGATCC G^GATCC BanI 1239 GGCACC G^G[CT][GA]CC BbsI 1490 GTCTTC GAAGAC(2/6) BciVI 1513 GTATCC GTATCC(6/5) BcnI 77 CCGGG CC^[GC]GG BpmI 1508 CTCCAG CTGGAG(16/14) BpuAI 1490 GTCTTC GAAGAC(2/6) BsaHI 210 GACGCC G[GA]^CG[CT]C BseRI 1752 CTCCTC GAGGAG(10/8) BsiHKAI 1308 GAGCTC G[AT]GC[AT]^C BsiYI 980 CCAGCCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 980 CCAGCCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1407I 1195 TGTACA T^GTACA BspHI 206 TCATGA T^CATGA BspLU11I 1048 ACATGT A^CATGT BsrGI 1195 TGTACA T^GTACA Bst1107I 1746 GTATAC GTA^TAC BstYI 954 GGATCC [GA]^GATC[CT] BstZ17I 1746 GTATAC GTA^TAC CfoI 486 GCGC GCG^C Eco47III 485 AGCGCT AGC^GCT FauI 1517 CCCGC CCCGC(4/6) HaeII 485 AGCGCT [GA]GCGC^[CT] HaeIII 1041 GGCC GG^CC HhaI 486 GCGC GCG^C Hin1I 210 GACGCC G[GA]^CG[CT]C HinP1I 486 GCGC G^CGC HincII 945 GTTGAC GT[CT]^[GA]AC HindII 945 GTTGAC GT[CT]^[GA]AC MaeII 236 ACGT A^CGT MflI 954 GGATCC [GA]^GATC[CT] MslI 1086 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1685 CCGCTG C[AC]G^C[GT]G NciI 77 CCGGG CC^[GC]GG NdeI 1145 CATATG CA^TATG NspI 1048 ACATGT [GA]CATG^[CT] PacI 931 TTAATTAA TTAAT^TAA PleI 1292 GAGTC GAGTC(4/5) PshAI 475 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 206 TCATGA T^CATGA SacI 1308 GAGCTC GAGCT^C SmlI 1305 CTTGAG C^T[CT][GA]AG SspBI 1195 TGTACA T^GTACA TfiI 909 GAATC G^A[AT]TC XbaI 922 TCTAGA T^CTAGA XhoII 954 GGATCC [GA]^GATC[CT] XmnI 827 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 1540 GTAGAC GT^[AC][GT]AC 1746 GTATAC AccIII 399 TCCGGA T^CCGGA 912 TCCGGA AocI 984 CCTAAGG CC^T[ACGT]AGG 1860 CCTAAGG AseI 748 ATTAAT AT^TAAT 1555 ATTAAT AsnI 748 ATTAAT AT^TAAT 1555 ATTAAT BanII 1040 GGGCCC G[GA]GC[CT]^C 1308 GAGCTC BclI 1710 TGATCA T^GATCA 1770 TGATCA BfaI 466 CTAG C^TAG 923 CTAG BsaWI 399 TCCGGA [AT]^CCGG[AT] 912 TCCGGA BseAI 399 TCCGGA T^CCGGA 912 TCCGGA BsgI 532 GTGCAG GTGCAG(16/14) 1781 GTGCAG BsmBI 1334 CGTCTC CGTCTC(1/5) 1730 CGTCTC BsmFI 342 GGGAC GGGAC(10/14) 565 GGGAC BspEI 399 TCCGGA T^CCGGA 912 TCCGGA BstEII 301 GGTCACC G^GT[ACGT]ACC 694 GGTTACC BstPI 301 GGTCACC G^GT[ACGT]ACC 694 GGTTACC Bsu36I 984 CCTAAGG CC^T[ACGT]AGG 1860 CCTAAGG DsaI 551 CCATGG C^C[GA][CT]GG 836 CCATGG Eco81I 984 CCTAAGG CC^T[ACGT]AGG 1860 CCTAAGG EcoO65I 301 GGTCACC G^GT[ACGT]ACC 694 GGTTACC EcoT14I 551 CCATGG C^C[AT][AT]GG 836 CCATGG FbaI 1710 TGATCA T^GATCA 1770 TGATCA HinfI 909 GAATC G^A[ACGT]TC 1292 GAGTC MaeI 466 CTAG C^TAG 923 CTAG MroI 399 TCCGGA T^CCGGA 912 TCCGGA NcoI 551 CCATGG C^CATGG 836 CCATGG PpuMI 342 GGGACCT [GA]G^G[AT]CC[CT] 1435 AGGACCT PshBI 748 ATTAAT AT^TAAT 1555 ATTAAT SapI 1417 GAAGAGC GCTCTTC(1/4) 382 GCTCTTC StyI 551 CCATGG C^C[AT][AT]GG 836 CCATGG TaqI 492 TCGA T^CGA 1295 TCGA TspRI 9 CAGTG CA[GC]TG[ACGT][ACGT]^ 963 CAGTG TthHB8I 492 TCGA T^CGA 1295 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BmyI BsaJI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HapII HgaI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I