(Circular) (Six-base) MAPSORT of: pFN21AE6495.seq Check: 7556 from: 1 to: 6079 pFN21AE6495 6079 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2023 2182 2023 Size: 159 5920 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4142 5526 5794 1632 Size: 2510 1384 268 1917 Fragments arranged by size: 2510 1917 1384 268 AfeI AGC'GCT Cuts at: 2760 2834 3180 2760 Size: 74 346 5659 Fragments arranged by size: 5659 346 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2364 2559 2988 4978 5955 1203 Size: 1161 195 429 1990 977 1327 Fragments arranged by size: 1990 1327 1161 977 429 195 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 2960 3191 1166 Size: 1794 231 4054 Fragments arranged by size: 4054 1794 231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2347 1184 Size: 1163 4916 AlwNI CAG_nnn'CTG Cuts at: 2026 2197 3822 5394 2026 Size: 171 1625 1572 2711 Fragments arranged by size: 2711 1625 1572 171 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 3388 3614 3766 5875 1493 Size: 1895 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 3404 1870 Size: 73 1461 4545 Fragments arranged by size: 4545 1461 73 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2272 2660 3260 3400 4855 Size: 325 217 627 485 388 600 140 1455 Cuts at: 4855 618 Size: 1842 Fragments arranged by size: 1842 1455 627 600 485 388 325 217 140 BanII G_rGCy'C Cuts at: 1391 3398 1391 Size: 2007 4072 BbeI G_GCGC'C Cuts at: 2276 2664 2276 Size: 388 5691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4270 4304 1903 Size: 34 2333 34 3678 Fragments arranged by size: 3678 2333 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 2450 2615 Size: 107 122 71 631 605 751 27 165 Cuts at: 2615 4690 136 Size: 2075 1525 Fragments arranged by size: 2075 1525 751 631 605 165 122 107 71 27 BglII A'GATC_T Cuts at: 6074 6074 Size: 6079 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2901 4140 5296 1790 Size: 1111 1239 1156 2573 Fragments arranged by size: 2573 1239 1156 1111 BmgBI CAC'GTC Cuts at: 1318 2310 2365 1318 Size: 992 55 5032 Fragments arranged by size: 5032 992 55 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3810 4768 442 Size: 416 53 83 399 24 2393 958 1753 Fragments arranged by size: 2393 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2539 2539 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2315 2534 2609 2878 4739 1239 Size: 1013 63 219 75 269 1861 2579 Fragments arranged by size: 2579 1861 1013 269 219 75 63 Bpu10I CC'TnA_GC Cuts at: 3275 3275 Size: 6079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4204 5069 5367 4204 Size: 865 298 4916 Fragments arranged by size: 4916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 4742 882 Size: 617 624 2619 2219 Fragments arranged by size: 2619 2219 624 617 BsaAI yAC'GTr Cuts at: 493 1204 2449 2715 3870 493 Size: 711 1245 266 1155 2702 Fragments arranged by size: 2702 1245 1155 711 266 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 2520 2661 2813 Size: 53 83 186 936 740 247 141 152 Cuts at: 2813 3016 3886 4268 4903 275 Size: 203 870 382 635 1451 Fragments arranged by size: 1451 936 870 740 635 382 247 203 186 152 141 83 53 BsaWI w'CCGG_w Cuts at: 4512 4910 5184 5331 5742 4512 Size: 398 274 147 411 4849 Fragments arranged by size: 4849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5854 Fragments arranged by size: 5854 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6079 BseYI C'CCAG_C Cuts at: 2465 2623 2683 2729 2842 5282 5778 2465 Size: 158 60 46 113 2440 496 2766 Fragments arranged by size: 2766 2440 496 158 113 60 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 3083 1163 Size: 573 315 1032 4159 Fragments arranged by size: 4159 1032 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2068 2209 2213 2343 2418 2481 Size: 275 186 67 141 4 130 75 63 Cuts at: 2481 2779 2889 2945 2949 3192 3529 4290 5318 Size: 298 110 56 4 243 337 761 1028 Cuts at: 5318 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 337 298 275 243 186 141 130 110 75 67 63 56 4 4 BsiHKAI G_wGCw'C Cuts at: 2088 2301 2489 4140 4225 5296 2088 Size: 213 188 1651 85 1071 2871 Fragments arranged by size: 2871 1651 1071 213 188 85 BsmI GAATG_Cn' Cuts at: 3590 3683 3590 Size: 93 5986 BsmBI CGTCTCn'nnnn_ Cuts at: 2927 2927 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2301 2489 2901 3398 4140 4225 Size: 399 298 213 188 412 497 742 85 Cuts at: 4225 5296 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 497 412 399 298 213 188 85 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2182 2233 2883 3434 844 Size: 1179 159 51 650 551 3489 Fragments arranged by size: 3489 1179 650 551 159 51 BsrBI CCG'CTC Cuts at: 1394 1823 2115 2381 3966 1394 Size: 429 292 266 1585 3507 Fragments arranged by size: 3507 1585 429 292 266 BsrDI GCAATG_nn' Cuts at: 65 1248 2324 4574 4748 65 Size: 1183 1076 2250 174 1396 Fragments arranged by size: 2250 1396 1183 1076 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 2212 2275 2765 2884 2891 2944 Size: 110 398 4 63 490 119 7 53 Cuts at: 2944 3287 4723 4910 1700 Size: 343 1436 187 2869 Fragments arranged by size: 2869 1436 490 398 343 187 119 110 63 53 7 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 2720 4139 5151 2720 Size: 1419 1012 3648 Fragments arranged by size: 3648 1419 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2197 794 Size: 1403 4676 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstXI CCAn_nnnn'nTGG Cuts at: 2915 2962 2915 Size: 47 6032 BstYI r'GATC_y Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074 Size: 1437 367 385 17 1452 86 12 346 Cuts at: 6074 1972 Size: 1977 Fragments arranged by size: 1977 1452 1437 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2433 3326 2433 Size: 893 5186 BtgI C'CryG_G Cuts at: 513 1065 2004 2826 2847 2961 3129 3192 3296 Size: 552 939 822 21 114 168 63 104 Cuts at: 3296 513 Size: 3296 Fragments arranged by size: 3296 939 822 552 168 114 104 63 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5340 Fragments arranged by size: 5340 683 56 BtsI GCAGTG_nn' Cuts at: 2982 3034 3100 3595 4389 4409 2982 Size: 52 66 495 794 20 4652 Fragments arranged by size: 4652 794 495 66 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DraIII CAC_nnn'GTG Cuts at: 2535 2535 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2210 2340 Size: 54 1425 50 275 18 217 163 130 Cuts at: 2340 2415 2478 2607 2616 2763 2776 2868 2886 Size: 75 63 129 9 147 13 92 18 Cuts at: 2886 2917 2942 2946 3003 3031 3126 3154 3189 Size: 31 25 4 57 28 95 28 35 Cuts at: 3189 3526 4415 6044 8 Size: 337 889 1629 43 Fragments arranged by size: 1629 1425 889 337 275 217 163 147 130 129 95 92 75 63 57 54 50 43 35 31 28 28 25 18 18 13 9 4 EagI C'GGCC_G Cuts at: 1537 1812 2210 2340 2415 2478 2776 2886 2942 Size: 275 398 130 75 63 298 110 56 Cuts at: 2942 2946 3189 3526 1537 Size: 4 243 337 4090 Fragments arranged by size: 4090 398 337 298 275 243 130 110 75 63 56 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2114 2390 2547 2930 3188 4009 1353 Size: 707 54 276 157 383 258 821 3423 Fragments arranged by size: 3423 821 707 383 276 258 157 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2240 4666 5040 5186 387 Size: 1064 138 651 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 651 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2315 2534 2609 2878 4142 4739 Size: 393 620 63 219 75 269 1264 597 Cuts at: 4739 5526 5794 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1264 787 620 597 393 269 268 219 75 63 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2546 2030 Size: 516 5563 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FseI GG_CCGG'CC Cuts at: 2948 2948 Size: 6079 FspI TGC'GCA Cuts at: 2323 3074 4585 2323 Size: 751 1511 3817 Fragments arranged by size: 3817 1511 751 HaeII r_GCGC'y Cuts at: 1678 2219 2276 2282 2429 2664 2762 2836 3149 Size: 541 57 6 147 235 98 74 313 Cuts at: 3149 3182 3345 5226 1678 Size: 33 163 1881 2531 Fragments arranged by size: 2531 1881 541 313 235 163 147 98 74 57 33 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4720 4752 4794 4826 1373 Size: 32 135 32 3148 32 42 32 2626 Fragments arranged by size: 3148 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3166 3223 3423 3669 669 Size: 988 281 1228 57 200 246 3079 Fragments arranged by size: 3079 1228 988 281 246 200 57 HindIII A'AGCT_T Cuts at: 748 2633 748 Size: 1885 4194 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KasI G'GCGC_C Cuts at: 2272 2660 2272 Size: 388 5691 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 2559 2988 5955 2559 Size: 429 2967 2683 Fragments arranged by size: 2967 2683 429 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 3111 5193 5377 1305 Size: 987 819 2082 184 2007 Fragments arranged by size: 2082 2007 987 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 3033 3128 10 Size: 54 1425 343 786 415 95 2961 Fragments arranged by size: 2961 1425 786 415 343 95 54 MslI CAynn'nnrTG Cuts at: 518 1166 2329 2960 3191 4037 4396 4555 518 Size: 648 1163 631 231 846 359 159 2042 Fragments arranged by size: 2042 1163 846 648 631 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2849 3298 3495 4172 5320 5565 1606 Size: 1243 449 197 677 1148 245 2120 Fragments arranged by size: 2120 1243 1148 677 449 245 197 NaeI GCC'GGC Cuts at: 2214 2277 2767 2886 2893 2946 3289 2214 Size: 63 490 119 7 53 343 5004 Fragments arranged by size: 5004 490 343 119 63 53 7 NarI GG'CG_CC Cuts at: 2273 2661 2273 Size: 388 5691 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NgoMIV G'CCGG_C Cuts at: 2212 2275 2765 2884 2891 2944 3287 2212 Size: 63 490 119 7 53 343 5004 Fragments arranged by size: 5004 490 343 119 63 53 7 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 2340 3526 2340 Size: 1186 4893 NspI r_CATG'y Cuts at: 1132 1229 2125 3232 3437 4982 1132 Size: 97 896 1107 205 1545 2229 Fragments arranged by size: 2229 1545 1107 896 205 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 1108 Size: 1932 4147 PfoI T'CCnGG_A Cuts at: 2511 2511 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PmlI CAC'GTG Cuts at: 2715 2715 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PstI C_TGCA'G Cuts at: 830 2193 2244 3431 830 Size: 1363 51 1187 3478 Fragments arranged by size: 3478 1363 1187 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 RsrII CG'GwC_CG Cuts at: 2982 2982 Size: 6079 SacII CC_GC'GG Cuts at: 2850 3299 2850 Size: 449 5630 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 3188 2060 Size: 1128 4951 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SfcI C'TryA_G Cuts at: 826 1045 2189 2240 3427 4562 5243 5434 826 Size: 219 1144 51 1187 1135 681 191 1471 Fragments arranged by size: 1471 1187 1144 1135 681 219 191 51 SfiI GGCCn_nnn'nGGCC Cuts at: 2615 2615 Size: 6079 SfoI GGC'GCC Cuts at: 2274 2662 2274 Size: 388 5691 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 2275 2891 1700 Size: 575 616 4888 Fragments arranged by size: 4888 616 575 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4183 5084 5346 820 Size: 197 926 2240 901 262 1553 Fragments arranged by size: 2240 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 2125 3437 1132 Size: 993 1312 3774 Fragments arranged by size: 3774 1312 993 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 2106 1517 Size: 589 5490 StyI C'CwwG_G Cuts at: 513 1065 2004 3518 513 Size: 552 939 1514 3074 Fragments arranged by size: 3074 1514 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 2999 4106 4123 4276 4461 4906 Size: 1255 449 970 1107 17 153 185 445 Cuts at: 4906 325 Size: 1498 Fragments arranged by size: 1498 1255 1107 970 449 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4325 6037 96 Size: 274 80 33 51 1443 2348 1712 138 Fragments arranged by size: 2348 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6039 6071 6039 Size: 32 6047 Tth111I GACn'n_nGTC Cuts at: 1316 3209 1316 Size: 1893 4186 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2602 2671 2824 2962 2602 Size: 69 153 138 5719 Fragments arranged by size: 5719 153 138 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoO109I EcoRV FalI FspAI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SexAI SrfI SwaI