(Circular) (Six-base) MAPSORT of: pFN21AE5297.seq Check: 4728 from: 1 to: 8116 pFN21AE5297 8116 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5926 278 Size: 53 83 186 936 4390 2468 Fragments arranged by size: 4390 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 5459 1937 Size: 739 2783 4594 Fragments arranged by size: 4594 2783 739 Acc65I G'GTAC_C Cuts at: 4407 5437 4407 Size: 1030 7086 AclI AA'CG_TT Cuts at: 1563 2732 6243 6616 1563 Size: 1169 3511 373 3063 Fragments arranged by size: 3511 3063 1169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 2572 2706 2755 3176 3532 6179 7563 Size: 728 212 134 49 421 356 2647 1384 Cuts at: 7563 7831 1632 Size: 268 1917 Fragments arranged by size: 2647 1917 1384 728 421 356 268 212 134 49 AfeI AGC'GCT Cuts at: 4230 4230 Size: 8116 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7919 AflIII A'CryG_T Cuts at: 1203 2640 2679 3996 4242 4261 7015 7992 1203 Size: 1437 39 1317 246 19 2754 977 1327 Fragments arranged by size: 2754 1437 1327 1317 977 246 39 19 AgeI A'CCGG_T Cuts at: 6947 6947 Size: 8116 AhdI GACnn_n'nnGTC Cuts at: 6845 6845 Size: 8116 AleI CACnn'nnGTG Cuts at: 1166 2639 3530 4001 4514 4848 1166 Size: 1473 891 471 513 334 4434 Fragments arranged by size: 4434 1473 891 513 471 334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8116 AlwNI CAG_nnn'CTG Cuts at: 2103 2434 3154 3514 4864 4918 5859 7431 2103 Size: 331 720 360 1350 54 941 1572 2788 Fragments arranged by size: 2788 1572 1350 941 720 360 331 54 ApaI G_GGCC'C Cuts at: 5109 5109 Size: 8116 ApaLI G'TGCA_C Cuts at: 3656 4639 6173 7329 3656 Size: 983 1534 1156 4443 Fragments arranged by size: 4443 1534 1156 983 ApoI r'AATT_y Cuts at: 1493 2471 4580 4595 5425 5651 5803 7912 1493 Size: 978 2109 15 830 226 152 2109 1697 Fragments arranged by size: 2109 2109 1697 978 830 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 2985 6670 160 Size: 872 1953 3685 1606 Fragments arranged by size: 3685 1953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 AvaI C'yCGr_G Cuts at: 1870 1943 3963 4290 4719 4834 5441 1870 Size: 73 2020 327 429 115 607 4545 Fragments arranged by size: 4545 2020 607 429 327 115 73 BamHI G'GATC_C Cuts at: 3039 5446 5813 3039 Size: 2407 367 5342 Fragments arranged by size: 5342 2407 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2555 3221 3634 4286 Size: 325 217 627 396 372 666 413 652 Cuts at: 4286 4407 5336 5437 6892 618 Size: 121 929 101 1455 1842 Fragments arranged by size: 1842 1455 929 666 652 627 413 396 372 325 217 121 101 BanII G_rGCy'C Cuts at: 1391 3602 4328 4955 5109 5435 1391 Size: 2211 726 627 154 326 4072 Fragments arranged by size: 4072 2211 726 627 326 154 BbeI G_GCGC'C Cuts at: 2559 3225 3638 5340 2559 Size: 666 413 1702 5335 Fragments arranged by size: 5335 1702 666 413 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 2695 3538 3801 4438 4981 928 Size: 971 257 539 843 263 637 543 4063 Fragments arranged by size: 4063 971 843 637 543 539 263 257 BbvCI CC'TCA_GC Cuts at: 2918 3494 5350 2918 Size: 576 1856 5684 Fragments arranged by size: 5684 1856 576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 6307 6341 1903 Size: 34 2363 34 1973 34 3678 Fragments arranged by size: 3678 2363 1973 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 6008 7218 3656 Size: 2352 1210 4554 Fragments arranged by size: 4554 2352 1210 BclI T'GATC_A Cuts at: 1548 2514 3825 1548 Size: 966 1311 5839 Fragments arranged by size: 5839 1311 966 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 6727 136 Size: 107 122 71 631 605 781 4274 1525 Fragments arranged by size: 4274 1525 781 631 605 122 107 71 BglII A'GATC_T Cuts at: 3369 8111 3369 Size: 4742 3374 BlpI GC'TnA_GC Cuts at: 3422 4100 4775 5533 3422 Size: 678 675 758 6005 Fragments arranged by size: 6005 758 678 675 Bme1580I G_kGCm'C Cuts at: 1790 3660 4289 4643 5109 6177 7333 1790 Size: 1870 629 354 466 1068 1156 2573 Fragments arranged by size: 2573 1870 1156 1068 629 466 354 BmgBI CAC'GTC Cuts at: 1318 2896 3999 1318 Size: 1578 1103 5435 Fragments arranged by size: 5435 1578 1103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5847 6805 442 Size: 416 53 83 399 24 4430 958 1753 Fragments arranged by size: 4430 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8116 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4354 4354 Size: 8116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2099 2591 4172 4571 4955 6776 1239 Size: 838 22 492 1581 399 384 1821 2579 Fragments arranged by size: 2579 1821 1581 838 492 399 384 22 Bpu10I CC'TnA_GC Cuts at: 2918 3494 5350 2918 Size: 576 1856 5684 Fragments arranged by size: 5684 1856 576 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6241 7106 7404 6241 Size: 865 298 6953 Fragments arranged by size: 6953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2718 4342 6779 882 Size: 617 1219 1624 2437 2219 Fragments arranged by size: 2437 2219 1624 1219 617 BsaAI yAC'GTr Cuts at: 493 1204 3418 4264 5907 493 Size: 711 2214 846 1643 2702 Fragments arranged by size: 2702 2214 1643 846 711 BsaBI GATnn'nnATC Cuts at: 8110 8110 Size: 8116 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2556 3222 3635 4563 Size: 53 83 186 936 1023 666 413 928 Cuts at: 4563 5337 5923 6305 6940 275 Size: 774 586 382 635 1451 Fragments arranged by size: 1451 1023 936 928 774 666 635 586 413 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 5253 6549 6947 7221 7368 7779 4092 Size: 1161 1296 398 274 147 411 4429 Fragments arranged by size: 4429 1296 1161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 3683 3713 4169 Size: 30 165 30 672 30 1578 30 456 Cuts at: 4169 4199 1178 Size: 30 5095 Fragments arranged by size: 5095 1578 672 456 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2465 3107 3482 4178 4265 5144 5147 Size: 1177 102 642 375 696 87 879 3 Cuts at: 5147 5327 1186 Size: 180 3975 Fragments arranged by size: 3975 1177 879 696 642 375 180 102 87 3 BseYI C'CCAG_C Cuts at: 2087 2551 4458 4767 4857 7319 7815 2087 Size: 464 1907 309 90 2462 496 2388 Fragments arranged by size: 2462 2388 1907 496 464 309 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 2645 3398 4639 4872 5150 1163 Size: 573 429 480 753 1241 233 278 4129 Fragments arranged by size: 4129 1241 753 573 480 429 278 233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5566 6327 7355 1540 Size: 275 186 3565 761 1028 2301 Fragments arranged by size: 3565 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3423 3660 4643 6177 6262 7333 3423 Size: 237 983 1534 85 1071 4206 Fragments arranged by size: 4206 1534 1071 983 237 85 BsmI GAATG_Cn' Cuts at: 2701 3579 5323 5627 5720 2701 Size: 878 1744 304 93 5097 Fragments arranged by size: 5097 1744 878 304 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4718 4718 Size: 8116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3423 3602 3660 4289 4328 4643 4955 Size: 399 1633 179 58 629 39 315 312 Cuts at: 4955 5109 5435 6177 6262 7333 1391 Size: 154 326 742 85 1071 2174 Fragments arranged by size: 2174 1633 1071 742 629 399 326 315 312 179 154 85 58 39 BspEI T'CCGG_A Cuts at: 7779 7779 Size: 8116 BspHI T'CATG_A Cuts at: 6005 7735 6005 Size: 1730 6386 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2604 3420 5471 844 Size: 1760 816 2051 3489 Fragments arranged by size: 3489 2051 1760 816 BsrBI CCG'CTC Cuts at: 1394 1823 2901 6003 1394 Size: 429 1078 3102 3507 Fragments arranged by size: 3507 3102 1078 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4545 6611 6785 65 Size: 1183 3297 2066 174 1396 Fragments arranged by size: 3297 2066 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2599 3241 4049 5068 5277 6760 6947 Size: 110 789 642 808 1019 209 1483 187 Cuts at: 6947 1700 Size: 2869 Fragments arranged by size: 2869 1483 1019 808 789 642 209 187 110 BsrGI T'GTAC_A Cuts at: 96 2643 3723 96 Size: 2547 1080 4489 Fragments arranged by size: 4489 2547 1080 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8116 BssSI C'ACGA_G Cuts at: 6176 7188 6176 Size: 1012 7104 BstAPI GCAn_nnn'nTGC Cuts at: 794 4024 794 Size: 3230 4886 BstBI TT'CG_AA Cuts at: 6920 6920 Size: 8116 BstEII G'GTnAC_C Cuts at: 1184 4522 1184 Size: 3338 4778 BstXI CCAn_nnnn'nTGG Cuts at: 2956 2956 Size: 8116 BstYI r'GATC_y Cuts at: 1972 2775 3039 3150 3369 3772 4059 5446 5813 Size: 803 264 111 219 403 287 1387 367 Cuts at: 5813 6198 6215 7667 7753 7765 8111 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1387 803 403 385 367 346 287 264 219 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4362 4362 Size: 8116 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2839 3225 3432 3574 3970 Size: 552 939 123 712 386 207 142 396 Cuts at: 3970 4209 4491 4527 4557 513 Size: 239 282 36 30 4072 Fragments arranged by size: 4072 939 712 552 396 386 282 239 207 142 123 36 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7377 Fragments arranged by size: 7377 683 56 BtsI GCAGTG_nn' Cuts at: 2761 3518 4622 5632 6426 6446 2761 Size: 757 1104 1010 794 20 4431 Fragments arranged by size: 4431 1104 1010 794 757 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2109 2144 529 Size: 35 1545 35 6501 Fragments arranged by size: 6501 1545 35 35 DraI TTT'AAA Cuts at: 2667 3034 5420 2667 Size: 367 2386 5363 Fragments arranged by size: 5363 2386 367 DraIII CAC_nnn'GTG Cuts at: 4267 4267 Size: 8116 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3275 4676 7123 809 Size: 507 1959 1401 2447 1802 Fragments arranged by size: 2447 1959 1802 1401 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3263 3534 4047 Size: 54 1425 50 275 18 1433 271 513 Cuts at: 4047 5070 5563 6452 8081 8 Size: 1023 493 889 1629 43 Fragments arranged by size: 1629 1433 1425 1023 889 513 493 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5563 1537 Size: 275 3751 4090 Fragments arranged by size: 4090 3751 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6046 1353 Size: 4693 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3068 3398 5264 6703 7077 7223 Size: 1064 138 1479 330 1866 1439 374 146 Cuts at: 7223 387 Size: 1280 Fragments arranged by size: 1866 1479 1439 1280 1064 374 330 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2099 2360 2572 2591 2706 2755 Size: 393 445 22 261 212 19 115 49 Cuts at: 2755 3176 3532 4172 4571 4955 6179 6776 7563 Size: 421 356 640 399 384 1224 597 787 Cuts at: 7563 7831 1239 Size: 268 1524 Fragments arranged by size: 1524 1224 787 640 597 445 421 399 393 384 356 268 261 212 115 49 22 19 EcoNI CCTnn'n_nnAGG Cuts at: 3047 3047 Size: 8116 EcoO109I rG'GnC_Cy Cuts at: 2263 4141 4716 5020 5106 2263 Size: 1878 575 304 86 5273 Fragments arranged by size: 5273 1878 575 304 86 EcoRI G'AATT_C Cuts at: 2471 4580 5425 5803 2471 Size: 2109 845 378 4784 Fragments arranged by size: 4784 2109 845 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2557 2557 Size: 8116 FspI TGC'GCA Cuts at: 6622 6622 Size: 8116 HaeII r_GCGC'y Cuts at: 1678 2559 3225 3638 4232 5340 7263 1678 Size: 881 666 413 594 1108 1923 2531 Fragments arranged by size: 2531 1923 1108 881 666 594 413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 3966 3998 6757 Size: 32 135 32 743 32 1619 32 2759 Cuts at: 6757 6789 6831 6863 1373 Size: 32 42 32 2626 Fragments arranged by size: 2759 2626 1619 743 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 5460 5706 669 Size: 988 281 798 2724 246 3079 Fragments arranged by size: 3079 2724 988 798 281 246 HindIII A'AGCT_T Cuts at: 748 3647 748 Size: 2899 5217 HpaI GTT'AAC Cuts at: 5706 5706 Size: 8116 KasI G'GCGC_C Cuts at: 2555 3221 3634 5336 2555 Size: 666 413 1702 5335 Fragments arranged by size: 5335 1702 666 413 KpnI G_GTAC'C Cuts at: 4411 5441 4411 Size: 1030 7086 MfeI C'AATT_G Cuts at: 5715 5715 Size: 8116 MluI A'CGCG_T Cuts at: 7992 7992 Size: 8116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4365 7230 7414 1305 Size: 3060 2865 184 2007 Fragments arranged by size: 3060 2865 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3265 3536 10 Size: 54 1425 343 1433 271 4590 Fragments arranged by size: 4590 1433 1425 343 271 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 3530 4001 4496 4514 4784 4848 Size: 648 1473 891 471 495 18 270 64 Cuts at: 4848 6074 6433 6592 518 Size: 1226 359 159 2042 Fragments arranged by size: 2042 1473 1226 891 648 495 471 359 270 159 64 18 MspA1I CmG'CkG Cuts at: 1606 2103 2629 2841 4402 4458 4514 4559 4771 Size: 497 526 212 1561 56 56 45 212 Cuts at: 4771 5026 5532 6209 7357 7602 1606 Size: 255 506 677 1148 245 2120 Fragments arranged by size: 2120 1561 1148 677 526 506 497 255 245 212 212 56 56 45 NaeI GCC'GGC Cuts at: 5070 5279 5070 Size: 209 7907 NarI GG'CG_CC Cuts at: 2556 3222 3635 5337 2556 Size: 666 413 1702 5335 Fragments arranged by size: 5335 1702 666 413 NcoI C'CATG_G Cuts at: 513 1065 2004 3432 3574 4209 4491 513 Size: 552 939 1428 142 635 282 4138 Fragments arranged by size: 4138 1428 939 635 552 282 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 8116 NgoMIV G'CCGG_C Cuts at: 5068 5277 5068 Size: 209 7907 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8116 NotI GC'GGCC_GC Cuts at: 5563 5563 Size: 8116 NspI r_CATG'y Cuts at: 1132 1229 2404 2683 3345 4246 5474 7019 1132 Size: 97 1175 279 662 901 1228 1545 2229 Fragments arranged by size: 2229 1545 1228 1175 901 662 279 97 PasI CC'CwG_GG Cuts at: 1348 2965 1348 Size: 1617 6499 PciI A'CATG_T Cuts at: 2679 4242 7015 2679 Size: 1563 2773 3780 Fragments arranged by size: 3780 2773 1563 PflMI CCAn_nnn'nTGG Cuts at: 1108 2434 4864 1108 Size: 1326 2430 4360 Fragments arranged by size: 4360 2430 1326 PfoI T'CCnGG_A Cuts at: 2568 4173 5076 2568 Size: 1605 903 5608 Fragments arranged by size: 5608 1605 903 PmeI GTTT'AAAC Cuts at: 5420 5420 Size: 8116 PmlI CAC'GTG Cuts at: 3418 4264 3418 Size: 846 7270 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6185 6217 7698 7730 6185 Size: 32 1481 32 6571 Fragments arranged by size: 6571 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 4141 4716 5020 2263 Size: 1878 575 304 5359 Fragments arranged by size: 5359 1878 575 304 PshAI GACnn'nnGTC Cuts at: 1613 5222 1613 Size: 3609 4507 PsiI TTA'TAA Cuts at: 5686 5686 Size: 8116 PspOMI G'GGCC_C Cuts at: 5105 5105 Size: 8116 PspXI vC'TCGA_Gb Cuts at: 4834 4834 Size: 8116 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5039 5071 5039 Size: 32 8084 PstI C_TGCA'G Cuts at: 830 2310 4555 4914 5031 5189 5211 5468 830 Size: 1480 2245 359 117 158 22 257 3478 Fragments arranged by size: 3478 2245 1480 359 257 158 117 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8116 PvuII CAG'CTG Cuts at: 2629 4514 4771 2629 Size: 1885 257 5974 Fragments arranged by size: 5974 1885 257 RsrII CG'GwC_CG Cuts at: 3055 3055 Size: 8116 SacII CC_GC'GG Cuts at: 2842 4560 2842 Size: 1718 6398 SalI G'TCGA_C Cuts at: 1936 5458 1936 Size: 3522 4594 SbfI CC_TGCA'GG Cuts at: 5468 5468 Size: 8116 ScaI AGT'ACT Cuts at: 6364 8076 6364 Size: 1712 6404 SexAI A'CCwGG_T Cuts at: 4898 4898 Size: 8116 SfcI C'TryA_G Cuts at: 826 1045 2306 4551 4910 5027 5185 5207 5464 Size: 219 1261 2245 359 117 158 22 257 Cuts at: 5464 6599 7280 7471 826 Size: 1135 681 191 1471 Fragments arranged by size: 2245 1471 1261 1135 681 359 257 219 191 158 117 22 SfoI GGC'GCC Cuts at: 2557 3223 3636 5338 2557 Size: 666 413 1702 5335 Fragments arranged by size: 5335 1702 666 413 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 SgrAI Cr'CCGG_yG Cuts at: 1700 5277 1700 Size: 3577 4539 SmaI CCC'GGG Cuts at: 1872 3965 4292 5443 1872 Size: 2093 327 1151 4545 Fragments arranged by size: 4545 2093 1151 327 SmlI C'TyrA_G Cuts at: 820 1017 1943 4834 6220 7121 7383 820 Size: 197 926 2891 1386 901 262 1553 Fragments arranged by size: 2891 1553 1386 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5907 493 Size: 5414 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8116 SphI G_CATG'C Cuts at: 1132 3345 5474 1132 Size: 2213 2129 3774 Fragments arranged by size: 3774 2213 2129 SspI AAT'ATT Cuts at: 5 52 2747 6040 5 Size: 47 2695 3293 2081 Fragments arranged by size: 3293 2695 2081 47 StuI AGG'CCT Cuts at: 1517 3473 4014 4418 1517 Size: 1956 541 404 5215 Fragments arranged by size: 5215 1956 541 404 StyI C'CwwG_G Cuts at: 513 1065 2004 2871 3432 3574 4209 4491 5555 Size: 552 939 867 561 142 635 282 1064 Cuts at: 5555 513 Size: 3074 Fragments arranged by size: 3074 1064 939 867 635 561 552 282 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2497 3072 3404 3578 4047 6143 6160 Size: 1255 917 575 332 174 469 2096 17 Cuts at: 6160 6313 6498 6943 325 Size: 153 185 445 1498 Fragments arranged by size: 2096 1498 1255 917 575 469 445 332 185 174 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 2643 3723 Size: 274 80 33 51 1443 339 327 1080 Cuts at: 3723 6362 8074 96 Size: 2639 1712 138 Fragments arranged by size: 2639 1712 1443 1080 339 327 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4622 4654 8076 8108 4622 Size: 32 3422 32 4630 Fragments arranged by size: 4630 3422 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3008 1316 Size: 1692 6424 XbaI T'CTAG_A Cuts at: 5452 5452 Size: 8116 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 2736 2790 5092 2221 Size: 515 54 2302 5245 Fragments arranged by size: 5245 2302 515 54 XhoI C'TCGA_G Cuts at: 1943 4834 1943 Size: 2891 5225 XmaI C'CCGG_G Cuts at: 1870 3963 4290 5441 1870 Size: 2093 327 1151 4545 Fragments arranged by size: 4545 2093 1151 327 XmnI GAAnn'nnTTC Cuts at: 2978 3161 6245 2978 Size: 183 3084 4849 Fragments arranged by size: 4849 3084 183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5924 276 Size: 53 83 186 936 4390 2468 Fragments arranged by size: 4390 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI SacI SanDI SapI SfiI SrfI SwaI