(Circular) (Six-base) MAPSORT of: pFN21AE6731.seq Check: 6125 from: 1 to: 8863 pFN21AE6731 8863 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3139 4054 3139 Size: 915 7948 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6673 278 Size: 53 83 186 936 5137 2468 Fragments arranged by size: 5137 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 2869 6206 1937 Size: 487 445 3337 4594 Fragments arranged by size: 4594 3337 487 445 Acc65I G'GTAC_C Cuts at: 3515 6184 3515 Size: 2669 6194 AclI AA'CG_TT Cuts at: 1563 6990 7363 1563 Size: 5427 373 3063 Fragments arranged by size: 5427 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 2775 3030 3173 3769 6926 8310 8578 Size: 654 489 255 143 596 3157 1384 268 Cuts at: 8578 1632 Size: 1917 Fragments arranged by size: 3157 1917 1384 654 596 489 268 255 143 AflII C'TTAA_G Cuts at: 820 1017 3611 820 Size: 197 2594 6072 Fragments arranged by size: 6072 2594 197 AflIII A'CryG_T Cuts at: 1203 3270 6023 7762 8739 1203 Size: 2067 2753 1739 977 1327 Fragments arranged by size: 2753 2067 1739 1327 977 AgeI A'CCGG_T Cuts at: 7694 7694 Size: 8863 AhdI GACnn_n'nnGTC Cuts at: 4168 7592 4168 Size: 3424 5439 AleI CACnn'nnGTG Cuts at: 1166 2432 2981 3242 1166 Size: 1266 549 261 6787 Fragments arranged by size: 6787 1266 549 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2970 1184 Size: 1786 7077 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4371 4403 4371 Size: 32 8831 AlwNI CAG_nnn'CTG Cuts at: 3083 3166 3388 3475 3797 4006 4136 5697 6606 Size: 83 222 87 322 209 130 1561 909 Cuts at: 6606 8178 3083 Size: 1572 3768 Fragments arranged by size: 3768 1572 1561 909 322 222 209 130 87 83 ApaI G_GGCC'C Cuts at: 5851 6086 5851 Size: 235 8628 ApaLI G'TGCA_C Cuts at: 2390 6920 8076 2390 Size: 4530 1156 3177 Fragments arranged by size: 4530 3177 1156 ApoI r'AATT_y Cuts at: 1493 6172 6398 6550 8659 1493 Size: 4679 226 152 2109 1697 Fragments arranged by size: 4679 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7417 160 Size: 872 6385 1606 Fragments arranged by size: 6385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8863 AvaI C'yCGr_G Cuts at: 1870 1943 2232 4619 4721 6003 6078 6188 1870 Size: 73 289 2387 102 1282 75 110 4545 Fragments arranged by size: 4545 2387 1282 289 110 102 75 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4345 4378 4345 Size: 33 8830 BamHI G'GATC_C Cuts at: 2332 2854 6193 6560 2332 Size: 522 3339 367 4635 Fragments arranged by size: 4635 3339 522 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2397 3515 4431 4510 Size: 325 217 627 579 31 1118 916 79 Cuts at: 4510 4746 5343 5397 5542 6014 6184 7639 618 Size: 236 597 54 145 472 170 1455 1842 Fragments arranged by size: 1842 1455 1118 916 627 597 579 472 325 236 217 170 145 79 54 31 BanII G_rGCy'C Cuts at: 1391 2244 3679 3924 4038 4620 4677 4728 4770 Size: 853 1435 245 114 582 57 51 42 Cuts at: 4770 5382 5851 6059 6077 6086 6182 1391 Size: 612 469 208 18 9 96 4072 Fragments arranged by size: 4072 1435 853 612 582 469 245 208 114 96 57 51 42 18 9 BbeI G_GCGC'C Cuts at: 2370 2401 4750 2370 Size: 31 2349 6483 Fragments arranged by size: 6483 2349 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3630 928 Size: 971 1731 6161 Fragments arranged by size: 6161 1731 971 BbvCI CC'TCA_GC Cuts at: 5648 5858 5648 Size: 210 8653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4648 4682 7054 7088 1903 Size: 34 50 34 2627 34 2372 34 3678 Fragments arranged by size: 3678 2627 2372 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5584 6755 7965 5584 Size: 1171 1210 6482 Fragments arranged by size: 6482 1210 1171 BclI T'GATC_A Cuts at: 1548 1548 Size: 8863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3199 4709 5551 Size: 107 122 71 631 605 1527 1510 842 Cuts at: 5551 7474 136 Size: 1923 1525 Fragments arranged by size: 1923 1527 1525 1510 842 631 605 122 107 71 BglII A'GATC_T Cuts at: 2218 4425 5131 8858 2218 Size: 2207 706 3727 2223 Fragments arranged by size: 3727 2223 2207 706 BlpI GC'TnA_GC Cuts at: 4769 6280 4769 Size: 1511 7352 Bme1580I G_kGCm'C Cuts at: 1790 2394 2571 4486 4515 4572 4579 5033 5348 Size: 604 177 1915 29 57 7 454 315 Cuts at: 5348 5547 5686 5851 5931 6017 6086 6924 8080 Size: 199 139 165 80 86 69 838 1156 Cuts at: 8080 1790 Size: 2573 Fragments arranged by size: 2573 1915 1156 838 604 454 315 199 177 165 139 86 80 69 57 29 7 BmgBI CAC'GTC Cuts at: 1318 3037 3765 1318 Size: 1719 728 6416 Fragments arranged by size: 6416 1719 728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3040 3171 3438 Size: 416 53 83 399 24 1623 131 267 Cuts at: 3438 3637 3654 3759 4082 4302 4566 4574 5649 Size: 199 17 105 323 220 264 8 1075 Cuts at: 5649 6594 7552 442 Size: 945 958 1753 Fragments arranged by size: 1753 1623 1075 958 945 416 399 323 267 264 220 199 131 105 83 53 24 17 8 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2794 4849 5341 5401 5580 2794 Size: 2055 492 60 179 6077 Fragments arranged by size: 6077 2055 492 179 60 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 2623 2789 3105 3341 3350 3476 3857 Size: 826 558 166 316 236 9 126 381 Cuts at: 3857 4194 4634 4646 4857 5145 5164 5336 5371 Size: 337 440 12 211 288 19 172 35 Cuts at: 5371 5906 6043 7523 1239 Size: 535 137 1480 2579 Fragments arranged by size: 2579 1480 826 558 535 440 381 337 316 288 236 211 172 166 137 126 35 19 12 9 Bpu10I CC'TnA_GC Cuts at: 5648 5858 5648 Size: 210 8653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2609 2669 6988 7853 8151 2609 Size: 60 4319 865 298 3321 Fragments arranged by size: 4319 3321 865 298 60 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2233 3104 4523 7526 882 Size: 617 734 871 1419 3003 2219 Fragments arranged by size: 3003 2219 1419 871 734 617 BsaAI yAC'GTr Cuts at: 493 1204 3271 6654 493 Size: 711 2067 3383 2702 Fragments arranged by size: 3383 2702 2067 711 BsaBI GATnn'nnATC Cuts at: 2648 8857 2648 Size: 6209 2654 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2398 4747 6670 Size: 53 83 186 936 834 31 2349 1923 Cuts at: 6670 7052 7687 275 Size: 382 635 1451 Fragments arranged by size: 2349 1923 1451 936 834 635 382 186 83 53 31 BsaWI w'CCGG_w Cuts at: 2179 2919 5751 7296 7694 7968 8115 8526 2179 Size: 740 2832 1545 398 274 147 411 2516 Fragments arranged by size: 2832 2516 1545 740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3078 3108 4317 4347 4371 Size: 30 165 30 1675 30 1209 30 24 Cuts at: 4371 4401 5187 5217 5416 5446 6085 6091 6115 Size: 30 786 30 199 30 639 6 24 Cuts at: 6115 6121 1178 Size: 6 3920 Fragments arranged by size: 3920 1675 1209 786 639 199 165 30 30 30 30 30 30 30 24 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 3391 4313 4511 5099 5148 5259 5321 Size: 912 1293 922 198 588 49 111 62 Cuts at: 5321 5324 5351 1186 Size: 3 27 4698 Fragments arranged by size: 4698 1293 922 912 588 198 111 62 49 27 3 BseYI C'CCAG_C Cuts at: 2360 3057 3260 3790 3923 4258 4395 4479 5074 Size: 697 203 530 133 335 137 84 595 Cuts at: 5074 5381 5496 5577 5587 5844 5940 8066 8562 Size: 307 115 81 10 257 96 2126 496 Cuts at: 8562 2360 Size: 2661 Fragments arranged by size: 2661 2126 697 595 530 496 335 307 257 203 137 133 115 96 84 81 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3266 3557 3851 4610 5246 1163 Size: 573 1530 291 294 759 636 4780 Fragments arranged by size: 4780 1530 759 636 573 294 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2411 5037 6036 6313 7074 8102 Size: 275 186 410 2626 999 277 761 1028 Cuts at: 8102 1540 Size: 2301 Fragments arranged by size: 2626 2301 1028 999 761 410 277 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 4770 5746 5952 6924 7009 8080 2394 Size: 2376 976 206 972 85 1071 3177 Fragments arranged by size: 3177 2376 1071 976 972 206 85 BsmI GAATG_Cn' Cuts at: 2567 4290 6374 6467 2567 Size: 1723 2084 93 4963 Fragments arranged by size: 4963 2084 1723 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3937 3937 Size: 8863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2394 2571 3679 3924 4038 4486 Size: 399 454 150 177 1108 245 114 448 Cuts at: 4486 4515 4572 4579 4620 4677 4728 4770 5033 Size: 29 57 7 41 57 51 42 263 Cuts at: 5033 5348 5382 5547 5686 5746 5851 5931 5952 Size: 315 34 165 139 60 105 80 21 Cuts at: 5952 6017 6059 6077 6086 6182 6924 7009 8080 Size: 65 42 18 9 96 742 85 1071 Cuts at: 8080 1391 Size: 2174 Fragments arranged by size: 2174 1108 1071 742 454 448 399 315 263 245 177 165 150 139 114 105 96 85 80 65 60 57 57 51 42 42 41 34 29 21 18 9 7 BspEI T'CCGG_A Cuts at: 2919 5751 8526 2919 Size: 2832 2775 3256 Fragments arranged by size: 3256 2832 2775 BspHI T'CATG_A Cuts at: 6752 8482 6752 Size: 1730 7133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 2898 3139 4054 6218 844 Size: 1181 873 241 915 2164 3489 Fragments arranged by size: 3489 2164 1181 915 873 241 BsrBI CCG'CTC Cuts at: 1394 1823 3603 5890 6750 1394 Size: 429 1780 2287 860 3507 Fragments arranged by size: 3507 2287 1780 860 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4447 4500 7358 7532 65 Size: 1183 3199 53 2858 174 1396 Fragments arranged by size: 3199 2858 1396 1183 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 4801 6035 7507 7694 1700 Size: 110 2991 1234 1472 187 2869 Fragments arranged by size: 2991 2869 1472 1234 187 110 BsrGI T'GTAC_A Cuts at: 96 3522 96 Size: 3426 5437 BssHII G'CGCG_C Cuts at: 1925 2403 1925 Size: 478 8385 BssSI C'ACGA_G Cuts at: 6923 7935 6923 Size: 1012 7851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2350 3475 794 Size: 1556 1125 6182 Fragments arranged by size: 6182 1556 1125 BstBI TT'CG_AA Cuts at: 7667 7667 Size: 8863 BstEII G'GTnAC_C Cuts at: 1184 3052 5404 1184 Size: 1868 2352 4643 Fragments arranged by size: 4643 2352 1868 BstXI CCAn_nnnn'nTGG Cuts at: 4045 4403 5340 4045 Size: 358 937 7568 Fragments arranged by size: 7568 937 358 BstYI r'GATC_y Cuts at: 1972 2218 2332 2854 2916 4425 5131 6193 6560 Size: 246 114 522 62 1509 706 1062 367 Cuts at: 6560 6945 6962 8414 8500 8512 8858 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1509 1452 1062 706 522 385 367 346 246 114 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 4137 4296 5125 4137 Size: 159 829 7875 Fragments arranged by size: 7875 829 159 BtgI C'CryG_G Cuts at: 513 1065 2575 2653 3240 3986 4857 5101 6030 Size: 552 1510 78 587 746 871 244 929 Cuts at: 6030 513 Size: 3346 Fragments arranged by size: 3346 1510 929 871 746 587 552 244 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2706 3511 487 Size: 683 56 1480 805 5839 Fragments arranged by size: 5839 1480 805 683 56 BtsI GCAGTG_nn' Cuts at: 2544 3476 3903 4812 5686 5905 6379 7173 7193 Size: 932 427 909 874 219 474 794 20 Cuts at: 7193 2544 Size: 4214 Fragments arranged by size: 4214 932 909 874 794 474 427 219 20 ClaI AT'CG_AT Cuts at: 1554 4465 1554 Size: 2911 5952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2918 2953 3030 3065 5799 5834 529 Size: 35 2354 35 77 35 2734 35 3558 Fragments arranged by size: 3558 2734 2354 77 35 35 35 35 DraI TTT'AAA Cuts at: 6167 6167 Size: 8863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2420 7870 809 Size: 507 1104 5450 1802 Fragments arranged by size: 5450 1802 1104 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2316 2408 Size: 54 1425 50 275 18 231 255 92 Cuts at: 2408 2578 3306 3507 3570 4545 5022 5034 5246 Size: 170 728 201 63 975 477 12 212 Cuts at: 5246 6033 6088 6310 7199 8828 8 Size: 787 55 222 889 1629 43 Fragments arranged by size: 1629 1425 975 889 787 728 477 275 255 231 222 212 201 170 92 63 55 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2408 5034 6033 6310 1537 Size: 275 596 2626 999 277 4090 Fragments arranged by size: 4090 2626 999 596 277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2378 2625 5775 5829 5958 6052 6793 1353 Size: 1025 247 3150 54 129 94 741 3423 Fragments arranged by size: 3423 3150 1025 741 247 129 94 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3002 4025 7450 7824 7970 387 Size: 1064 138 1413 1023 3425 374 146 1280 Fragments arranged by size: 3425 1413 1280 1064 1023 374 146 138 EcoICRI GAG'CTC Cuts at: 4768 4768 Size: 8863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2286 2623 2775 2789 3030 3105 Size: 393 433 221 337 152 14 241 75 Cuts at: 3105 3173 3341 3350 3476 3769 3857 4194 4634 Size: 68 168 9 126 293 88 337 440 Cuts at: 4634 4646 4857 5145 5164 5336 5371 5906 6043 Size: 12 211 288 19 172 35 535 137 Cuts at: 6043 6926 7523 8310 8578 1239 Size: 883 597 787 268 1524 Fragments arranged by size: 1524 883 787 597 535 440 433 393 337 337 293 288 268 241 221 211 172 168 152 137 126 88 75 68 35 19 14 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2840 3308 4140 5779 2840 Size: 468 832 1639 5924 Fragments arranged by size: 5924 1639 832 468 EcoO109I rG'GnC_Cy Cuts at: 3163 3313 3910 3982 4049 4133 4215 4303 4828 Size: 150 597 72 67 84 82 88 525 Cuts at: 4828 5051 5138 5735 5848 6082 6083 3163 Size: 223 87 597 113 234 1 5943 Fragments arranged by size: 5943 597 597 525 234 223 150 113 88 87 84 82 72 67 1 EcoRI G'AATT_C Cuts at: 6172 6550 6172 Size: 378 8485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5225 5225 Size: 8863 FspI TGC'GCA Cuts at: 2343 2789 7369 2343 Size: 446 4580 3837 Fragments arranged by size: 4580 3837 446 FspAI rTGC'GCAy Cuts at: 2343 2343 Size: 8863 HaeII r_GCGC'y Cuts at: 1678 2123 2370 2401 3205 4715 4750 8010 1678 Size: 445 247 31 804 1510 35 3260 2531 Fragments arranged by size: 3260 2531 1510 804 445 247 35 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3064 3096 4154 4186 5221 Size: 32 135 32 1492 32 1058 32 1035 Cuts at: 5221 5253 6085 6091 6117 6123 7504 7536 7578 Size: 32 832 6 26 6 1381 32 42 Cuts at: 7578 7610 1373 Size: 32 2626 Fragments arranged by size: 2626 1492 1381 1058 1035 832 135 42 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 6207 6453 669 Size: 988 281 4269 246 3079 Fragments arranged by size: 4269 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5207 5992 748 Size: 4459 785 3619 Fragments arranged by size: 4459 3619 785 HpaI GTT'AAC Cuts at: 6453 6453 Size: 8863 KasI G'GCGC_C Cuts at: 2366 2397 4746 2366 Size: 31 2349 6483 Fragments arranged by size: 6483 2349 31 KpnI G_GTAC'C Cuts at: 3519 6188 3519 Size: 2669 6194 MfeI C'AATT_G Cuts at: 6462 6462 Size: 8863 MluI A'CGCG_T Cuts at: 8739 8739 Size: 8863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 2745 4140 6083 7977 8161 1305 Size: 795 645 1395 1943 1894 184 2007 Fragments arranged by size: 2007 1943 1894 1395 795 645 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 3308 3509 3572 5248 Size: 54 1425 343 486 990 201 63 1676 Cuts at: 5248 10 Size: 3625 Fragments arranged by size: 3625 1676 1425 990 486 343 201 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2705 2952 2981 3242 5958 6821 Size: 648 1266 273 247 29 261 2716 863 Cuts at: 6821 7180 7339 518 Size: 359 159 2042 Fragments arranged by size: 2716 2042 1266 863 648 359 273 261 247 159 29 MspA1I CmG'CkG Cuts at: 1606 2364 2577 2753 3242 3564 3797 3835 3855 Size: 758 213 176 489 322 233 38 20 Cuts at: 3855 4096 4594 4695 5078 5944 6279 6956 8104 Size: 241 498 101 383 866 335 677 1148 Cuts at: 8104 8349 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 866 758 677 498 489 383 335 322 245 241 233 213 176 101 38 20 NaeI GCC'GGC Cuts at: 6037 6037 Size: 8863 NarI GG'CG_CC Cuts at: 2367 2398 4747 2367 Size: 31 2349 6483 Fragments arranged by size: 6483 2349 31 NcoI C'CATG_G Cuts at: 513 1065 3986 4857 5101 513 Size: 552 2921 871 244 4275 Fragments arranged by size: 4275 2921 871 552 244 NdeI CA'TA_TG Cuts at: 387 387 Size: 8863 NgoMIV G'CCGG_C Cuts at: 6035 6035 Size: 8863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8863 NotI GC'GGCC_GC Cuts at: 6310 6310 Size: 8863 NspI r_CATG'y Cuts at: 1132 1229 2704 2951 5515 5878 6027 6221 7766 Size: 97 1475 247 2564 363 149 194 1545 Cuts at: 7766 1132 Size: 2229 Fragments arranged by size: 2564 2229 1545 1475 363 247 194 149 97 PasI CC'CwG_GG Cuts at: 1348 4938 1348 Size: 3590 5273 PciI A'CATG_T Cuts at: 6023 7762 6023 Size: 1739 7124 PflMI CCAn_nnn'nTGG Cuts at: 1108 3141 3749 3797 1108 Size: 2033 608 48 6174 Fragments arranged by size: 6174 2033 608 48 PfoI T'CCnGG_A Cuts at: 2518 2600 3082 4070 2518 Size: 82 482 988 7311 Fragments arranged by size: 7311 988 482 82 PmeI GTTT'AAAC Cuts at: 6167 6167 Size: 8863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4371 4403 6932 6964 8445 8477 4371 Size: 32 2529 32 1481 32 4757 Fragments arranged by size: 4757 2529 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3163 3982 4049 4215 5051 5138 5735 3163 Size: 819 67 166 836 87 597 6291 Fragments arranged by size: 6291 836 819 597 166 87 67 PshAI GACnn'nnGTC Cuts at: 1613 5455 1613 Size: 3842 5021 PsiI TTA'TAA Cuts at: 6433 6433 Size: 8863 PspOMI G'GGCC_C Cuts at: 5847 6082 5847 Size: 235 8628 PstI C_TGCA'G Cuts at: 830 2037 3422 3480 3760 3864 4032 4065 4474 Size: 1207 1385 58 280 104 168 33 409 Cuts at: 4474 5094 5635 6215 830 Size: 620 541 580 3478 Fragments arranged by size: 3478 1385 1207 620 580 541 409 280 168 104 58 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8863 PvuII CAG'CTG Cuts at: 2753 3564 3797 3835 4096 4594 2753 Size: 811 233 38 261 498 7022 Fragments arranged by size: 7022 811 498 261 233 38 SacI G_AGCT'C Cuts at: 4770 4770 Size: 8863 SacII CC_GC'GG Cuts at: 2578 3243 2578 Size: 665 8198 SalI G'TCGA_C Cuts at: 1936 6205 1936 Size: 4269 4594 SanDI GG'GwC_CC Cuts at: 4049 4215 5138 4049 Size: 166 923 7774 Fragments arranged by size: 7774 923 166 SapI GCTCTTCn'nnn_ Cuts at: 5775 5775 Size: 8863 SbfI CC_TGCA'GG Cuts at: 4032 4065 6215 4032 Size: 33 2150 6680 Fragments arranged by size: 6680 2150 33 ScaI AGT'ACT Cuts at: 2556 4189 7111 8823 2556 Size: 1633 2922 1712 2596 Fragments arranged by size: 2922 2596 1712 1633 SfcI C'TryA_G Cuts at: 826 1045 2033 3418 3476 3559 3651 3756 3860 Size: 219 988 1385 58 83 92 105 104 Cuts at: 3860 4028 4061 4166 4470 5059 5090 5631 5776 Size: 168 33 105 304 589 31 541 145 Cuts at: 5776 6211 7346 8027 8218 826 Size: 435 1135 681 191 1471 Fragments arranged by size: 1471 1385 1135 988 681 589 541 435 304 219 191 168 145 105 105 104 92 83 58 33 31 SfoI GGC'GCC Cuts at: 2368 2399 4748 2368 Size: 31 2349 6483 Fragments arranged by size: 6483 2349 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8863 SmaI CCC'GGG Cuts at: 1872 4621 6005 6080 6190 1872 Size: 2749 1384 75 110 4545 Fragments arranged by size: 4545 2749 1384 110 75 SmlI C'TyrA_G Cuts at: 820 1017 1943 2624 2684 3611 6967 7868 8130 Size: 197 926 681 60 927 3356 901 262 Cuts at: 8130 820 Size: 1553 Fragments arranged by size: 3356 1553 927 926 901 681 262 197 60 SnaBI TAC'GTA Cuts at: 493 6654 493 Size: 6161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8863 SphI G_CATG'C Cuts at: 1132 6221 1132 Size: 5089 3774 SrfI GCCC'GGGC Cuts at: 4621 4621 Size: 8863 SspI AAT'ATT Cuts at: 5 52 6787 5 Size: 47 6735 2081 Fragments arranged by size: 6735 2081 47 StuI AGG'CCT Cuts at: 1517 3782 5608 5969 1517 Size: 2265 1826 361 4411 Fragments arranged by size: 4411 2265 1826 361 StyI C'CwwG_G Cuts at: 513 1065 3309 3986 4243 4338 4857 4864 5101 Size: 552 2244 677 257 95 519 7 237 Cuts at: 5101 5190 5548 5763 6302 513 Size: 89 358 215 539 3074 Fragments arranged by size: 3074 2244 677 552 539 519 358 257 237 215 95 89 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4870 4923 5609 5970 6890 6907 7060 Size: 1255 3290 53 686 361 920 17 153 Cuts at: 7060 7245 7690 325 Size: 185 445 1498 Fragments arranged by size: 3290 1498 1255 920 686 445 361 185 153 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2554 3522 4187 Size: 274 80 33 51 1443 577 968 665 Cuts at: 4187 7109 8821 96 Size: 2922 1712 138 Fragments arranged by size: 2922 1712 1443 968 665 577 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 2599 2631 3078 3110 3455 3487 4317 Size: 32 53 32 447 32 345 32 830 Cuts at: 4317 4349 5187 5219 8823 8855 2514 Size: 32 838 32 3604 32 2522 Fragments arranged by size: 3604 2522 838 830 447 345 53 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8863 XbaI T'CTAG_A Cuts at: 6199 6199 Size: 8863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3316 3385 4558 4855 5180 5694 3316 Size: 69 1173 297 325 514 6485 Fragments arranged by size: 6485 1173 514 325 297 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8863 XmaI C'CCGG_G Cuts at: 1870 4619 6003 6078 6188 1870 Size: 2749 1384 75 110 4545 Fragments arranged by size: 4545 2749 1384 110 75 XmnI GAAnn'nnTTC Cuts at: 2045 6992 2045 Size: 4947 3916 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6671 276 Size: 53 83 186 936 5137 2468 Fragments arranged by size: 5137 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoRV FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SwaI