(Circular) (Six-base) MAPSORT of: pFN21AE5775.seq Check: 9580 from: 1 to: 6577 pFN21AE5775 6577 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2111 2111 Size: 6577 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4387 278 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3920 1937 Size: 1983 4594 Acc65I G'GTAC_C Cuts at: 2822 3898 2822 Size: 1076 5501 AclI AA'CG_TT Cuts at: 1563 4704 5077 1563 Size: 3141 373 3063 Fragments arranged by size: 3141 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2611 2810 3233 3269 3311 4640 6024 6292 Size: 979 199 423 36 42 1329 1384 268 Cuts at: 6292 1632 Size: 1917 Fragments arranged by size: 1917 1384 1329 979 423 268 199 42 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6380 AflIII A'CryG_T Cuts at: 1203 2484 5476 6453 1203 Size: 1281 2992 977 1327 Fragments arranged by size: 2992 1327 1281 977 AgeI A'CCGG_T Cuts at: 5408 5408 Size: 6577 AhdI GACnn_n'nnGTC Cuts at: 2857 5306 2857 Size: 2449 4128 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6577 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2463 1184 Size: 1279 5298 AlwNI CAG_nnn'CTG Cuts at: 2164 3762 4320 5892 2164 Size: 1598 558 1572 2849 Fragments arranged by size: 2849 1598 1572 558 ApaLI G'TGCA_C Cuts at: 2524 4634 5790 2524 Size: 2110 1156 3311 Fragments arranged by size: 3311 2110 1156 ApoI r'AATT_y Cuts at: 1493 2041 2243 2253 2321 2899 3317 3553 3886 Size: 548 202 10 68 578 418 236 333 Cuts at: 3886 4112 4264 6373 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 578 548 418 333 236 226 202 152 68 10 AseI AT'TA_AT Cuts at: 160 1032 5131 160 Size: 872 4099 1606 Fragments arranged by size: 4099 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 AvaI C'yCGr_G Cuts at: 1870 1943 2130 3681 3873 3902 1870 Size: 73 187 1551 192 29 4545 Fragments arranged by size: 4545 1551 192 187 73 29 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2814 2847 2814 Size: 33 6544 BamHI G'GATC_C Cuts at: 2134 3907 4274 2134 Size: 1773 367 4437 Fragments arranged by size: 4437 1773 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2822 3898 5353 618 Size: 325 217 627 229 806 1076 1455 1842 Fragments arranged by size: 1842 1455 1076 806 627 325 229 217 BanII G_rGCy'C Cuts at: 1391 2472 3296 3896 1391 Size: 1081 824 600 4072 Fragments arranged by size: 4072 1081 824 600 BbeI G_GCGC'C Cuts at: 2020 2020 Size: 6577 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2946 3218 3368 928 Size: 971 1047 272 150 4137 Fragments arranged by size: 4137 1047 971 272 150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4768 4802 1903 Size: 34 2831 34 3678 Fragments arranged by size: 3678 2831 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2499 3609 4469 5679 2499 Size: 1110 860 1210 3397 Fragments arranged by size: 3397 1210 1110 860 BclI T'GATC_A Cuts at: 1548 2248 1548 Size: 700 5877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 5188 136 Size: 107 122 71 631 605 454 3062 1525 Fragments arranged by size: 3062 1525 631 605 454 122 107 71 BglII A'GATC_T Cuts at: 3036 6572 3036 Size: 3536 3041 BlpI GC'TnA_GC Cuts at: 3302 3994 3302 Size: 692 5885 Bme1580I G_kGCm'C Cuts at: 1790 2528 4638 5794 1790 Size: 738 2110 1156 2573 Fragments arranged by size: 2573 2110 1156 738 BmgBI CAC'GTC Cuts at: 1318 2485 1318 Size: 1167 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2648 3826 4308 Size: 416 53 83 399 24 1231 1178 482 Cuts at: 4308 5266 442 Size: 958 1753 Fragments arranged by size: 1753 1231 1178 958 482 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6577 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 3535 5237 1239 Size: 854 1442 1702 2579 Fragments arranged by size: 2579 1702 1442 854 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2960 4702 5567 5865 2960 Size: 1742 865 298 3672 Fragments arranged by size: 3672 1742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5240 882 Size: 617 3741 2219 Fragments arranged by size: 3741 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 4368 493 Size: 711 1167 1997 2702 Fragments arranged by size: 2702 1997 1167 711 BsaBI GATnn'nnATC Cuts at: 6571 6571 Size: 6577 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2017 4384 4766 5401 Size: 53 83 186 936 484 2367 382 635 Cuts at: 5401 275 Size: 1451 Fragments arranged by size: 2367 1451 936 635 484 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5010 5408 5682 5829 6240 5010 Size: 398 274 147 411 5347 Fragments arranged by size: 5347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3039 3069 1178 Size: 30 165 30 1636 30 4686 Fragments arranged by size: 4686 1636 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6577 BseYI C'CCAG_C Cuts at: 2208 3804 5780 6276 2208 Size: 1596 1976 496 2509 Fragments arranged by size: 2509 1976 1596 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2188 2216 2358 3095 1163 Size: 573 452 28 142 737 4645 Fragments arranged by size: 4645 737 573 452 142 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2121 4027 4788 5816 1540 Size: 275 186 120 1906 761 1028 2301 Fragments arranged by size: 2301 1906 1028 761 275 186 120 BsiHKAI G_wGCw'C Cuts at: 2087 2528 3296 4638 4723 5794 2087 Size: 441 768 1342 85 1071 2870 Fragments arranged by size: 2870 1342 1071 768 441 85 BsmI GAATG_Cn' Cuts at: 3255 4088 4181 3255 Size: 833 93 5651 Fragments arranged by size: 5651 833 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2472 2528 3296 3896 4638 4723 Size: 399 297 385 56 768 600 742 85 Cuts at: 4723 5794 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 768 742 600 399 385 297 85 56 BspEI T'CCGG_A Cuts at: 6240 6240 Size: 6577 BspHI T'CATG_A Cuts at: 3175 4466 6196 3175 Size: 1291 1730 3556 Fragments arranged by size: 3556 1730 1291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2111 3932 844 Size: 1267 1821 3489 Fragments arranged by size: 3489 1821 1267 BsrBI CCG'CTC Cuts at: 1394 1823 2060 4464 1394 Size: 429 237 2404 3507 Fragments arranged by size: 3507 2404 429 237 BsrDI GCAATG_nn' Cuts at: 65 1248 2751 3281 5072 5246 65 Size: 1183 1503 530 1791 174 1396 Fragments arranged by size: 1791 1503 1396 1183 530 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5221 5408 1700 Size: 110 3411 187 2869 Fragments arranged by size: 3411 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6577 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6577 BssSI C'ACGA_G Cuts at: 2234 4637 5649 2234 Size: 2403 1012 3162 Fragments arranged by size: 3162 2403 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2164 794 Size: 1370 5207 BstBI TT'CG_AA Cuts at: 5381 5381 Size: 6577 BstEII G'GTnAC_C Cuts at: 1184 2642 1184 Size: 1458 5119 BstXI CCAn_nnnn'nTGG Cuts at: 2145 2145 Size: 6577 BstYI r'GATC_y Cuts at: 1972 2134 3036 3394 3907 4274 4659 4676 6128 Size: 162 902 358 513 367 385 17 1452 Cuts at: 6128 6214 6226 6572 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 902 513 385 367 358 346 162 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2451 513 Size: 552 939 447 4639 Fragments arranged by size: 4639 939 552 447 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5838 Fragments arranged by size: 5838 683 56 BtsI GCAGTG_nn' Cuts at: 2165 3233 3693 4093 4887 4907 2165 Size: 1068 460 400 794 20 3835 Fragments arranged by size: 3835 1068 794 460 400 20 ClaI AT'CG_AT Cuts at: 1554 2181 1554 Size: 627 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6542 DraI TTT'AAA Cuts at: 3583 3881 3583 Size: 298 6279 DraIII CAC_nnn'GTG Cuts at: 3676 3676 Size: 6577 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5584 809 Size: 507 4268 1802 Fragments arranged by size: 4268 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 3025 4024 Size: 54 1425 50 275 18 288 907 999 Cuts at: 4024 4913 6542 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 999 907 889 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2118 4024 1537 Size: 275 306 1906 4090 Fragments arranged by size: 4090 1906 306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2983 4507 1353 Size: 1630 1524 3423 Fragments arranged by size: 3423 1630 1524 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5164 5538 5684 387 Size: 1064 138 3575 374 146 1280 Fragments arranged by size: 3575 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3294 3294 Size: 6577 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2611 2810 3233 3269 3311 3535 Size: 393 461 518 199 423 36 42 224 Cuts at: 3535 4640 5237 6024 6292 1239 Size: 1105 597 787 268 1524 Fragments arranged by size: 1524 1105 787 597 518 461 423 393 268 224 199 42 36 EcoNI CCTnn'n_nnAGG Cuts at: 2604 2604 Size: 6577 EcoO109I rG'GnC_Cy Cuts at: 3796 3796 Size: 6577 EcoRI G'AATT_C Cuts at: 3886 4264 3886 Size: 378 6199 FspI TGC'GCA Cuts at: 5083 5083 Size: 6577 HaeII r_GCGC'y Cuts at: 1678 2020 2116 5724 1678 Size: 342 96 3608 2531 Fragments arranged by size: 3608 2531 342 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2843 2875 3039 3071 3083 Size: 32 135 32 1271 32 164 32 12 Cuts at: 3083 3115 5218 5250 5292 5324 1373 Size: 32 2103 32 42 32 2626 Fragments arranged by size: 2626 2103 1271 164 135 42 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3921 4167 669 Size: 988 281 1983 246 3079 Fragments arranged by size: 3079 1983 988 281 246 HindIII A'AGCT_T Cuts at: 748 3006 748 Size: 2258 4319 HpaI GTT'AAC Cuts at: 4167 4167 Size: 6577 KasI G'GCGC_C Cuts at: 2016 2016 Size: 6577 KpnI G_GTAC'C Cuts at: 2826 3902 2826 Size: 1076 5501 MfeI C'AATT_G Cuts at: 4176 4176 Size: 6577 MluI A'CGCG_T Cuts at: 6453 6453 Size: 6577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5691 5875 1305 Size: 4386 184 2007 Fragments arranged by size: 4386 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3027 10 Size: 54 1425 343 1195 3560 Fragments arranged by size: 3560 1425 1195 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2716 3861 4535 4894 5053 518 Size: 648 1550 1145 674 359 159 2042 Fragments arranged by size: 2042 1550 1145 674 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2499 2864 3993 4670 5818 6063 1606 Size: 606 287 365 1129 677 1148 245 2120 Fragments arranged by size: 2120 1148 1129 677 606 365 287 245 NarI GG'CG_CC Cuts at: 2017 2017 Size: 6577 NcoI C'CATG_G Cuts at: 513 1065 2004 2451 513 Size: 552 939 447 4639 Fragments arranged by size: 4639 939 552 447 NdeI CA'TA_TG Cuts at: 387 387 Size: 6577 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6577 NotI GC'GGCC_GC Cuts at: 4024 4024 Size: 6577 NspI r_CATG'y Cuts at: 1132 1229 3068 3935 5480 1132 Size: 97 1839 867 1545 2229 Fragments arranged by size: 2229 1839 1545 867 97 PasI CC'CwG_GG Cuts at: 1348 2461 1348 Size: 1113 5464 PciI A'CATG_T Cuts at: 5476 5476 Size: 6577 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6577 PfoI T'CCnGG_A Cuts at: 2703 2983 2703 Size: 280 6297 PmeI GTTT'AAAC Cuts at: 3881 3881 Size: 6577 PmlI CAC'GTG Cuts at: 2371 2371 Size: 6577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4646 4678 6159 6191 4646 Size: 32 1481 32 5032 Fragments arranged by size: 5032 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6577 PsiI TTA'TAA Cuts at: 4147 4147 Size: 6577 PstI C_TGCA'G Cuts at: 830 2160 3929 830 Size: 1330 1769 3478 Fragments arranged by size: 3478 1769 1330 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6577 PvuII CAG'CTG Cuts at: 2212 2499 2864 2212 Size: 287 365 5925 Fragments arranged by size: 5925 365 287 SacI G_AGCT'C Cuts at: 3296 3296 Size: 6577 SalI G'TCGA_C Cuts at: 1936 3919 1936 Size: 1983 4594 SbfI CC_TGCA'GG Cuts at: 3929 3929 Size: 6577 ScaI AGT'ACT Cuts at: 4825 6537 4825 Size: 1712 4865 SfcI C'TryA_G Cuts at: 826 1045 2156 3766 3925 5060 5741 5932 826 Size: 219 1111 1610 159 1135 681 191 1471 Fragments arranged by size: 1610 1471 1135 1111 681 219 191 159 SfoI GGC'GCC Cuts at: 2018 2018 Size: 6577 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6577 SmaI CCC'GGG Cuts at: 1872 3904 1872 Size: 2032 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2975 4681 5582 5844 820 Size: 197 926 1032 1706 901 262 1553 Fragments arranged by size: 1706 1553 1032 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4368 493 Size: 3875 2702 SpeI A'CTAG_T Cuts at: 152 3428 152 Size: 3276 3301 SphI G_CATG'C Cuts at: 1132 3935 1132 Size: 2803 3774 SspI AAT'ATT Cuts at: 5 52 4501 5 Size: 47 4449 2081 Fragments arranged by size: 4449 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6577 StyI C'CwwG_G Cuts at: 513 1065 2004 2451 3193 4016 513 Size: 552 939 447 742 823 3074 Fragments arranged by size: 3074 939 823 742 552 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2222 3818 4604 4621 4774 4959 5404 Size: 1255 642 1596 786 17 153 185 445 Cuts at: 5404 325 Size: 1498 Fragments arranged by size: 1596 1498 1255 786 642 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3386 4823 6535 Size: 274 80 33 51 1443 1409 1437 1712 Cuts at: 6535 96 Size: 138 Fragments arranged by size: 1712 1443 1437 1409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6537 6569 6537 Size: 32 6545 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6577 XbaI T'CTAG_A Cuts at: 3259 3913 3259 Size: 654 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2458 2458 Size: 6577 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6577 XmaI C'CCGG_G Cuts at: 1870 3902 1870 Size: 2032 4545 XmnI GAAnn'nnTTC Cuts at: 2943 4706 2943 Size: 1763 4814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4385 276 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI