################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Aor51HI ApaI AscI Asp700I Asp718I AspEI BbrPI BcgI BciVI BclI BfrI BglI BglII BsaBI BsaI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRI EcoT22I FbaI FseI HgaI HindIII HpaI KpnI KspI MamI MluI MroI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccI 933 GTCGAC GT^[AC][GT]AC AcyI 1430 GGCGCC G[GA]^CG[CT]C Alw44I 10 GTGCAC G^TGCAC AlwNI 652 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 10 GTGCAC G^TGCAC AseI 868 ATTAAT AT^TAAT AsnI 868 ATTAAT AT^TAAT AspI 1245 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 450 CCTAGG C^CTAGG BamHI 1210 GGATCC G^GATCC BbeI 1430 GGCGCC GGCGC^C BbvCI 1474 CCTCAGC CCTCAGC(-5/-2) BbvI 1099 GCTGC GCAGC(8/12) BcnI 379 CCCGG CC^[GC]GG BfaI 451 CTAG C^TAG BlnI 450 CCTAGG C^CTAGG BlpI 1062 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1062 GCTGAGC GC^T[ACGT]AGC BsaAI 628 TACGTG [CT]AC^GT[GA] BsaHI 1430 GGCGCC G[GA]^CG[CT]C BsaWI 335 ACCGGA [AT]^CCGG[AT] BseRI 1399 GAGGAG GAGGAG(10/8) BsgI 601 CTGCAC GTGCAG(16/14) BsmI 1216 GCATTC GAATGC(1/-1) BspHI 44 TCATGA T^CATGA BssSI 30 CACGAG CACGAG(-5/-1) BstXI 275 CCATGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1210 GGATCC [GA]^GATC[CT] CelII 1062 GCTGAGC GC^T[ACGT]AGC DpnII 1211 GATC ^GATC DraIII 721 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1503 GACAAATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 1482 CCCGC CCCGC(4/6) HaeII 1430 GGCGCC [GA]GCGC^[CT] Hin1I 1430 GGCGCC G[GA]^CG[CT]C KasI 1430 GGCGCC G^GCGCC MaeI 451 CTAG C^TAG MboI 1211 GATC ^GATC MfeI 774 CAATTG C^AATTG MflI 1210 GGATCC [GA]^GATC[CT] MslI 276 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 411 CAGCGG C[AC]G^C[GT]G MunI 774 CAATTG C^AATTG NarI 1430 GGCGCC GG^CGCC NciI 379 CCCGG CC^[GC]GG NdeI 89 CATATG CA^TATG NdeII 1211 GATC ^GATC NspI 539 GCATGT [GA]CATG^[CT] PaeR7I 1382 CTCGAG C^TCGAG PflFI 1245 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 868 ATTAAT AT^TAAT RcaI 44 TCATGA T^CATGA SalI 933 GTCGAC G^TCGAC Sau3AI 1211 GATC ^GATC ScaI 1086 AGTACT AGT^ACT SfcI 743 CTACAG C^T[GA][CT]AG SfoI 1430 GGCGCC GGC^GCC TfiI 765 GATTC G^A[AT]TC TseI 1099 GCTGC G^C[AT]GC Tth111I 1245 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 148 CCACCCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1382 CTCGAG C^TCGAG XhoII 1210 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 70 GAATTT [GA]^AATT[CT] 339 GAATTT AlwI 1211 GATCC GGATC(4/5) 1210 GGATC AocI 1110 CCTGAGG CC^T[ACGT]AGG 1160 CCTCAGG ApoI 70 GAATTT [GA]^AATT[CT] 339 GAATTT AviII 856 TGCGCA TGC^GCA 965 TGCGCA BalI 668 TGGCCA TGG^CCA 1016 TGGCCA BanI 633 GGTGCC G^G[CT][GA]CC 1430 GGCGCC BbsI 190 GAAGAC GAAGAC(2/6) 546 GAAGAC BpuAI 190 GAAGAC GAAGAC(2/6) 546 GAAGAC BspMI 599 ACCTGC ACCTGC(4/8) 219 GCAGGT BstEII 222 GGTGACC G^GT[ACGT]ACC 807 GGTGACC BstF5I 1421 CATCC GGATG(2/0) 1265 GGATG BstPI 222 GGTGACC G^GT[ACGT]ACC 807 GGTGACC Bsu36I 1110 CCTGAGG CC^T[ACGT]AGG 1160 CCTCAGG DsaI 62 CCATGG C^C[GA][CT]GG 950 CCATGG EaeI 668 TGGCCA [CT]^GGCC[GA] 1016 TGGCCA Eco81I 1110 CCTGAGG CC^T[ACGT]AGG 1160 CCTCAGG EcoNI 132 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 213 CCTCCAGCAGG EcoO65I 222 GGTGACC G^GT[ACGT]ACC 807 GGTGACC EcoRV 184 GATATC GAT^ATC 406 GATATC FokI 1421 CATCC GGATG(9/13) 1265 GGATG FspI 856 TGCGCA TGC^GCA 965 TGCGCA HapII 336 CCGG C^CGG 380 CCGG HincII 514 GTCAAC GT[CT]^[GA]AC 933 GTCGAC HindII 514 GTCAAC GT[CT]^[GA]AC 933 GTCGAC HpaII 336 CCGG C^CGG 380 CCGG HphI 222 GGTGA GGTGA(8/7) 807 GGTGA MluNI 668 TGGCCA TGG^CCA 1016 TGGCCA MscI 668 TGGCCA TGG^CCA 1016 TGGCCA MspI 336 CCGG C^CGG 380 CCGG NcoI 62 CCATGG C^CATGG 950 CCATGG StuI 707 AGGCCT AGG^CCT 1471 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI AvaII BanII BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI ItaI Ksp632I MaeII MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV PflMI PleI RsaI SacI SapI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I Van91I