(Circular) (Six-base) MAPSORT of: pFN21AE3537.seq Check: 5961 from: 1 to: 6223 pFN21AE3537 6223 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2215 2215 Size: 6223 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 3566 1937 Size: 1002 627 4594 Fragments arranged by size: 4594 1002 627 Acc65I G'GTAC_C Cuts at: 3544 3544 Size: 6223 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4286 5670 5938 1632 Size: 2654 1384 268 1917 Fragments arranged by size: 2654 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 5122 6099 1203 Size: 3919 977 1327 Fragments arranged by size: 3919 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6223 AlwNI CAG_nnn'CTG Cuts at: 2662 3966 5538 2662 Size: 1304 1572 3347 Fragments arranged by size: 3347 1572 1304 ApaLI G'TGCA_C Cuts at: 2015 4280 5436 2015 Size: 2265 1156 2802 Fragments arranged by size: 2802 2265 1156 ApoI r'AATT_y Cuts at: 1493 2075 2344 3532 3758 3910 6019 1493 Size: 582 269 1188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1188 582 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 2874 4777 160 Size: 872 1842 1903 1606 Fragments arranged by size: 1903 1842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2700 2984 3387 3548 1870 Size: 73 757 284 403 161 4545 Fragments arranged by size: 4545 757 403 284 161 73 AvrII C'CTAG_G Cuts at: 2455 2455 Size: 6223 BamHI G'GATC_C Cuts at: 3215 3553 3920 3215 Size: 338 367 5518 Fragments arranged by size: 5518 367 338 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2638 3435 3544 4999 618 Size: 325 217 627 851 797 109 1455 1842 Fragments arranged by size: 1842 1455 851 797 627 325 217 109 BanII G_rGCy'C Cuts at: 1391 2622 2685 3000 3045 3466 3542 1391 Size: 1231 63 315 45 421 76 4072 Fragments arranged by size: 4072 1231 421 315 76 63 45 BbeI G_GCGC'C Cuts at: 3439 3439 Size: 6223 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2558 928 Size: 971 303 356 4593 Fragments arranged by size: 4593 971 356 303 BbvCI CC'TCA_GC Cuts at: 3480 3480 Size: 6223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4414 4448 1903 Size: 34 2477 34 3678 Fragments arranged by size: 3678 2477 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4834 136 Size: 107 122 71 631 605 3162 1525 Fragments arranged by size: 3162 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6218 6218 Size: 6223 BlpI GC'TnA_GC Cuts at: 3068 3640 3068 Size: 572 5651 Bme1580I G_kGCm'C Cuts at: 1790 2019 2643 3174 4284 5440 1790 Size: 229 624 531 1110 1156 2573 Fragments arranged by size: 2573 1156 1110 624 531 229 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3136 3247 3404 Size: 416 53 83 399 24 1719 111 157 Cuts at: 3404 3954 4912 442 Size: 550 958 1753 Fragments arranged by size: 1753 1719 958 550 416 399 157 111 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3480 3480 Size: 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2005 2204 3097 4883 1239 Size: 766 199 893 1786 2579 Fragments arranged by size: 2579 1786 893 766 199 Bpu10I CC'TnA_GC Cuts at: 3480 3480 Size: 6223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2741 3450 4348 5213 5511 2741 Size: 709 898 865 298 3453 Fragments arranged by size: 3453 898 865 709 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4886 882 Size: 617 3387 2219 Fragments arranged by size: 3387 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 4014 493 Size: 711 1431 1379 2702 Fragments arranged by size: 2702 1431 1379 711 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 4030 4412 5047 Size: 53 83 186 936 1903 594 382 635 Cuts at: 5047 275 Size: 1451 Fragments arranged by size: 1903 1451 936 635 594 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2340 4656 5054 5328 5475 5886 2340 Size: 2316 398 274 147 411 2677 Fragments arranged by size: 2677 2316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5998 Fragments arranged by size: 5998 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3419 1186 Size: 2233 3990 BseYI C'CCAG_C Cuts at: 2798 3306 5426 5922 2798 Size: 508 2120 496 3099 Fragments arranged by size: 3099 2120 508 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 1163 Size: 573 855 4795 Fragments arranged by size: 4795 855 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3673 4434 5462 1540 Size: 275 186 1672 761 1028 2301 Fragments arranged by size: 2301 1672 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 2685 3000 3466 4284 4369 5440 2019 Size: 666 315 466 818 85 1071 2802 Fragments arranged by size: 2802 1071 818 666 466 315 85 BsmI GAATG_Cn' Cuts at: 3221 3734 3827 3221 Size: 513 93 5617 Fragments arranged by size: 5617 513 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2622 2643 2685 3000 3045 3174 Size: 399 229 603 21 42 315 45 129 Cuts at: 3174 3466 3542 4284 4369 5440 1391 Size: 292 76 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 315 292 229 129 85 76 45 42 21 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 2049 4112 5842 2049 Size: 2063 1730 2430 Fragments arranged by size: 2430 2063 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2613 3578 844 Size: 1371 398 965 3489 Fragments arranged by size: 3489 1371 965 398 BsrBI CCG'CTC Cuts at: 1394 1823 4110 1394 Size: 429 2287 3507 Fragments arranged by size: 3507 2287 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4718 4892 65 Size: 1183 3470 174 1396 Fragments arranged by size: 3470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4867 5054 1700 Size: 110 3057 187 2869 Fragments arranged by size: 3057 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 2035 4283 5295 2035 Size: 2248 1012 2963 Fragments arranged by size: 2963 2248 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6223 BstBI TT'CG_AA Cuts at: 5027 5027 Size: 6223 BstEII G'GTnAC_C Cuts at: 1184 2227 2812 1184 Size: 1043 585 4595 Fragments arranged by size: 4595 1043 585 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2287 Size: 6223 BstYI r'GATC_y Cuts at: 1972 3215 3553 3920 4305 4322 5774 5860 5872 Size: 1243 338 367 385 17 1452 86 12 Cuts at: 5872 6218 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1243 385 367 346 338 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3116 3166 3116 Size: 50 6173 BtgI C'CryG_G Cuts at: 513 1065 2067 2955 513 Size: 552 1002 888 3781 Fragments arranged by size: 3781 1002 888 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 3739 4533 4553 3739 Size: 794 20 5409 Fragments arranged by size: 5409 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DraIII CAC_nnn'GTG Cuts at: 2731 2731 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3514 5230 809 Size: 507 2198 1716 1802 Fragments arranged by size: 2198 1802 1716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2673 3021 3670 Size: 54 1425 50 275 18 843 348 649 Cuts at: 3670 4559 6188 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 843 649 348 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 2690 3094 4153 1353 Size: 1016 321 404 1059 3423 Fragments arranged by size: 3423 1059 1016 404 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2322 4810 5184 5330 387 Size: 1064 138 733 2488 374 146 1280 Fragments arranged by size: 2488 1280 1064 733 374 146 138 EcoICRI GAG'CTC Cuts at: 2683 2998 3464 2683 Size: 315 466 5442 Fragments arranged by size: 5442 466 315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2204 3097 4286 4883 5670 5938 Size: 393 373 199 893 1189 597 787 268 Cuts at: 5938 1239 Size: 1524 Fragments arranged by size: 1524 1189 893 787 597 393 373 268 199 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2222 2141 Size: 81 6142 EcoRI G'AATT_C Cuts at: 3532 3910 3532 Size: 378 5845 EcoRV GAT'ATC Cuts at: 2191 2413 2191 Size: 222 6001 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2153 2153 Size: 6223 FspI TGC'GCA Cuts at: 2863 2972 4729 2863 Size: 109 1757 4357 Fragments arranged by size: 4357 1757 109 FspAI rTGC'GCAy Cuts at: 2972 2972 Size: 6223 HaeII r_GCGC'y Cuts at: 1678 3439 5370 1678 Size: 1761 1931 2531 Fragments arranged by size: 2531 1931 1761 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3496 3528 4864 4896 4938 Size: 32 135 32 1924 32 1336 32 42 Cuts at: 4938 4970 1373 Size: 32 2626 Fragments arranged by size: 2626 1924 1336 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2521 2940 3567 3813 669 Size: 988 281 583 419 627 246 3079 Fragments arranged by size: 3079 988 627 583 419 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6223 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KasI G'GCGC_C Cuts at: 3435 3435 Size: 6223 KpnI G_GTAC'C Cuts at: 3548 3548 Size: 6223 MfeI C'AATT_G Cuts at: 2779 3822 2779 Size: 1043 5180 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 5337 5521 1305 Size: 1161 2871 184 2007 Fragments arranged by size: 2871 2007 1161 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 3023 10 Size: 54 1425 343 843 348 3210 Fragments arranged by size: 3210 1425 843 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 4181 4540 4699 518 Size: 648 1119 1896 359 159 2042 Fragments arranged by size: 2042 1896 1119 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2418 3639 4316 5464 5709 1606 Size: 812 1221 677 1148 245 2120 Fragments arranged by size: 2120 1221 1148 812 677 245 NarI GG'CG_CC Cuts at: 3436 3436 Size: 6223 NcoI C'CATG_G Cuts at: 513 1065 2067 2955 513 Size: 552 1002 888 3781 Fragments arranged by size: 3781 1002 888 552 NdeI CA'TA_TG Cuts at: 387 2095 387 Size: 1708 4515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 2548 3581 5126 1132 Size: 97 1319 1033 1545 2229 Fragments arranged by size: 2229 1545 1319 1033 97 PasI CC'CwG_GG Cuts at: 1348 3045 3265 1348 Size: 1697 220 4306 Fragments arranged by size: 4306 1697 220 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2163 2402 2628 2651 1108 Size: 1055 239 226 23 4680 Fragments arranged by size: 4680 1055 239 226 23 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6223 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PstI C_TGCA'G Cuts at: 830 3575 830 Size: 2745 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 SacI G_AGCT'C Cuts at: 2685 3000 3466 2685 Size: 315 466 5442 Fragments arranged by size: 5442 466 315 SalI G'TCGA_C Cuts at: 1936 2938 3565 1936 Size: 1002 627 4594 Fragments arranged by size: 4594 1002 627 SapI GCTCTTCn'nnn_ Cuts at: 2369 2690 3094 2369 Size: 321 404 5498 Fragments arranged by size: 5498 404 321 SbfI CC_TGCA'GG Cuts at: 3575 3575 Size: 6223 ScaI AGT'ACT Cuts at: 3093 4471 6183 3093 Size: 1378 1712 3133 Fragments arranged by size: 3133 1712 1378 SfcI C'TryA_G Cuts at: 826 1045 2748 3571 4706 5387 5578 826 Size: 219 1703 823 1135 681 191 1471 Fragments arranged by size: 1703 1471 1135 823 681 219 191 SfoI GGC'GCC Cuts at: 3437 3437 Size: 6223 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 3550 1872 Size: 1678 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 3387 3465 4327 5228 5490 Size: 197 926 813 631 78 862 901 262 Cuts at: 5490 820 Size: 1553 Fragments arranged by size: 1553 926 901 862 813 631 262 197 78 SnaBI TAC'GTA Cuts at: 493 4014 493 Size: 3521 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 2714 3478 1517 Size: 1197 764 4262 Fragments arranged by size: 4262 1197 764 StyI C'CwwG_G Cuts at: 513 1065 2067 2116 2142 2157 2455 2955 3662 Size: 552 1002 49 26 15 298 500 707 Cuts at: 3662 513 Size: 3074 Fragments arranged by size: 3074 1002 707 552 500 298 49 26 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2170 4250 4267 4420 4605 5050 325 Size: 1255 590 2080 17 153 185 445 1498 Fragments arranged by size: 2080 1498 1255 590 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2535 3091 3383 Size: 274 80 33 51 1443 558 556 292 Cuts at: 3383 4469 6181 96 Size: 1086 1712 138 Fragments arranged by size: 1712 1443 1086 558 556 292 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6183 6215 6183 Size: 32 6191 Tth111I GACn'n_nGTC Cuts at: 1316 3253 1316 Size: 1937 4286 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2160 2160 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 3387 1943 Size: 1444 4779 XmaI C'CCGG_G Cuts at: 1870 3548 1870 Size: 1678 4545 XmnI GAAnn'nnTTC Cuts at: 4352 4352 Size: 6223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoO109I FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI