(Linear) (Six-base) MAPSORT of: KIEE6485.seq Check: 4481 from: 1 to: 1866 KIEE6485.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 1034 1866 Size: 1034 832 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1235 1254 1866 Size: 1235 19 612 Fragments arranged by size: 1235 612 19 AlwNI CAG_nnn'CTG Cuts at: 0 1255 1749 1866 Size: 1255 494 117 Fragments arranged by size: 1255 494 117 ApaLI G'TGCA_C Cuts at: 0 83 1866 Size: 83 1783 ApoI r'AATT_y Cuts at: 0 179 345 1866 Size: 179 166 1521 Fragments arranged by size: 1521 179 166 BanII G_rGCy'C Cuts at: 0 22 875 1122 1866 Size: 22 853 247 744 Fragments arranged by size: 853 744 247 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 958 1723 1866 Size: 958 765 143 Fragments arranged by size: 958 765 143 BciVI GTATCCnnnnn_n' Cuts at: 0 913 1866 Size: 913 953 BclI T'GATC_A Cuts at: 0 1356 1866 Size: 1356 510 Bme1580I G_kGCm'C Cuts at: 0 87 1866 Size: 87 1779 BmrI ACTGGGnnnn_n' Cuts at: 0 372 675 1866 Size: 372 303 1191 Fragments arranged by size: 1191 372 303 BmtI G_CTAG'C Cuts at: 0 1579 1866 Size: 1579 287 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 391 1866 Size: 41 350 1475 Fragments arranged by size: 1475 350 41 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 344 1866 Size: 344 1522 BseYI C'CCAG_C Cuts at: 0 15 1836 1866 Size: 15 1821 30 Fragments arranged by size: 1821 30 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 437 1866 Size: 48 389 1429 Fragments arranged by size: 1429 389 48 BsiHKAI G_wGCw'C Cuts at: 0 87 875 1866 Size: 87 788 991 Fragments arranged by size: 991 788 87 Bsp1286I G_dGCh'C Cuts at: 0 22 87 875 1122 1866 Size: 22 65 788 247 744 Fragments arranged by size: 788 744 247 65 22 BsrGI T'GTAC_A Cuts at: 0 1282 1866 Size: 1282 584 BstXI CCAn_nnnn'nTGG Cuts at: 0 665 1866 Size: 665 1201 BstYI r'GATC_y Cuts at: 0 285 1462 1866 Size: 285 1177 404 Fragments arranged by size: 1177 404 285 BstZ17I GTA'TAC Cuts at: 0 1035 1866 Size: 1035 831 Bsu36I CC'TnA_GG Cuts at: 0 1711 1866 Size: 1711 155 BtgI C'CryG_G Cuts at: 0 42 1436 1823 1866 Size: 42 1394 387 43 Fragments arranged by size: 1394 387 43 42 BtsI GCAGTG_nn' Cuts at: 0 31 1320 1362 1866 Size: 31 1289 42 504 Fragments arranged by size: 1289 504 42 31 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 414 449 1866 Size: 414 35 1417 Fragments arranged by size: 1417 414 35 DraI TTT'AAA Cuts at: 0 344 608 956 1866 Size: 344 264 348 910 Fragments arranged by size: 910 348 344 264 EarI CTCTTCn'nnn_ Cuts at: 0 128 362 880 1866 Size: 128 234 518 986 Fragments arranged by size: 986 518 234 128 EcoICRI GAG'CTC Cuts at: 0 873 1866 Size: 873 993 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 391 1235 1254 1866 Size: 41 350 844 19 612 Fragments arranged by size: 844 612 350 41 19 EcoRV GAT'ATC Cuts at: 0 732 1866 Size: 732 1134 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 982 1866 Size: 982 884 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 62 94 206 238 1454 1486 1795 1827 Size: 62 32 112 32 1216 32 309 32 Cuts at: 1827 1866 Size: 39 Fragments arranged by size: 1216 309 112 62 39 32 32 32 32 HincII GTy'rAC Cuts at: 0 1601 1866 Size: 1601 265 HindIII A'AGCT_T Cuts at: 0 703 1866 Size: 703 1163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 396 1866 Size: 396 1470 MslI CAynn'nnrTG Cuts at: 0 41 1325 1866 Size: 41 1284 541 Fragments arranged by size: 1284 541 41 NcoI C'CATG_G Cuts at: 0 42 1436 1823 1866 Size: 42 1394 387 43 Fragments arranged by size: 1394 387 43 42 NdeI CA'TA_TG Cuts at: 0 800 1691 1866 Size: 800 891 175 Fragments arranged by size: 891 800 175 NheI G'CTAG_C Cuts at: 0 1575 1866 Size: 1575 291 NspI r_CATG'y Cuts at: 0 486 1866 Size: 486 1380 PflMI CCAn_nnn'nTGG Cuts at: 0 64 1866 Size: 64 1802 PfoI T'CCnGG_A Cuts at: 0 57 1866 Size: 57 1809 PsiI TTA'TAA Cuts at: 0 1297 1866 Size: 1297 569 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1018 1050 1266 1298 1866 Size: 1018 32 216 32 568 Fragments arranged by size: 1018 568 216 32 32 PstI C_TGCA'G Cuts at: 0 863 1676 1866 Size: 863 813 190 Fragments arranged by size: 863 813 190 SacI G_AGCT'C Cuts at: 0 875 1866 Size: 875 991 SapI GCTCTTCn'nnn_ Cuts at: 0 128 880 1866 Size: 128 752 986 Fragments arranged by size: 986 752 128 SfcI C'TryA_G Cuts at: 0 859 1672 1866 Size: 859 813 194 Fragments arranged by size: 859 813 194 StyI C'CwwG_G Cuts at: 0 42 1003 1436 1552 1823 1866 Size: 42 961 433 116 271 43 Fragments arranged by size: 961 433 271 116 43 42 SwaI ATTT'AAAT Cuts at: 0 344 1866 Size: 344 1522 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 322 1850 1866 Size: 322 1528 16 Fragments arranged by size: 1528 322 16 TatI w'GTAC_w Cuts at: 0 1282 1817 1866 Size: 1282 535 49 Fragments arranged by size: 1282 535 49 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 506 538 1866 Size: 506 32 1328 Fragments arranged by size: 1328 506 32 Tth111I GACn'n_nGTC Cuts at: 0 157 1704 1866 Size: 157 1547 162 Fragments arranged by size: 1547 162 157 XmnI GAAnn'nnTTC Cuts at: 0 122 1866 Size: 122 1744 Enzymes that do cut: AccI AcuI AlwNI ApaLI ApoI BanII BbsI BciVI BclI Bme1580I BmrI BmtI BpmI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrGI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI EarI EcoICRI Eco57MI EcoRV FalI Hin4I HincII HindIII MmeI MslI NcoI NdeI NheI NspI PflMI PfoI PsiI PsrI PstI SacI SapI SfcI StyI SwaI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BtgZI ClaI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI XbaI XcmI XhoI XmaI ZraI