(Circular) (Six-base) MAPSORT of: pFN21AE5477.seq Check: 8022 from: 1 to: 5692 pFN21AE5477 5692 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2920 2920 Size: 5692 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 2740 3013 2740 Size: 273 5419 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2756 3755 5139 5407 1632 Size: 1124 999 1384 268 1917 Fragments arranged by size: 1917 1384 1124 999 268 AflII C'TTAA_G Cuts at: 820 1017 2762 820 Size: 197 1745 3750 Fragments arranged by size: 3750 1745 197 AflIII A'CryG_T Cuts at: 1203 2777 4591 5568 1203 Size: 1574 1814 977 1327 Fragments arranged by size: 1814 1574 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2154 1184 Size: 970 4722 AlwNI CAG_nnn'CTG Cuts at: 3435 5007 3435 Size: 1572 4120 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 2823 3001 3227 3379 5488 1493 Size: 1330 178 226 152 2109 1697 Fragments arranged by size: 2109 1697 1330 226 178 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 3017 1870 Size: 73 1074 4545 Fragments arranged by size: 4545 1074 73 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2740 3013 4468 618 Size: 325 217 627 953 273 1455 1842 Fragments arranged by size: 1842 1455 953 627 325 273 217 BanII G_rGCy'C Cuts at: 1391 3011 1391 Size: 1620 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2800 2948 928 Size: 971 901 148 3672 Fragments arranged by size: 3672 971 901 148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BfrBI ATG'CAT Cuts at: 2375 2375 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 3753 4909 1790 Size: 1963 1156 2573 Fragments arranged by size: 2573 1963 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3423 4381 442 Size: 416 53 83 399 24 2006 958 1753 Fragments arranged by size: 2006 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2258 1056 Size: 1202 4490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2092 2708 4352 1239 Size: 853 616 1644 2579 Fragments arranged by size: 2579 1644 853 616 Bpu10I CC'TnA_GC Cuts at: 2057 2057 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2883 2913 1178 Size: 30 165 30 1480 30 3957 Fragments arranged by size: 3957 1480 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2272 2371 2891 1186 Size: 1086 99 520 3987 Fragments arranged by size: 3987 1086 520 99 BseYI C'CCAG_C Cuts at: 2082 4895 5391 2082 Size: 2813 496 2383 Fragments arranged by size: 2813 2383 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 1163 Size: 573 538 4581 Fragments arranged by size: 4581 573 538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 2970 3753 3838 4909 2313 Size: 657 783 85 1071 3096 Fragments arranged by size: 3096 1071 783 657 85 BsmI GAATG_Cn' Cuts at: 2375 3203 3296 2375 Size: 828 93 4771 Fragments arranged by size: 4771 828 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2970 3011 3753 3838 4909 1391 Size: 399 523 657 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 523 399 85 41 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2920 3047 844 Size: 2076 127 3489 Fragments arranged by size: 3489 2076 127 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 4187 4361 65 Size: 1183 940 1999 174 1396 Fragments arranged by size: 1999 1396 1183 940 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4336 4523 1700 Size: 110 2526 187 2869 Fragments arranged by size: 2869 2526 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 2327 3752 4764 2327 Size: 1425 1012 3255 Fragments arranged by size: 3255 1425 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2269 2333 2416 2545 2011 Size: 258 64 83 129 5158 Fragments arranged by size: 5158 258 129 83 64 BstYI r'GATC_y Cuts at: 1972 2952 3022 3389 3774 3791 5243 5329 5341 Size: 980 70 367 385 17 1452 86 12 Cuts at: 5341 5687 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 980 385 367 346 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2385 2409 513 Size: 552 1320 24 3796 Fragments arranged by size: 3796 1320 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 2141 2286 2313 2736 3208 4002 4022 2141 Size: 145 27 423 472 794 20 3811 Fragments arranged by size: 3811 794 472 423 145 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2406 2694 2886 Size: 54 1425 50 275 18 576 288 192 Cuts at: 2886 3139 4028 5657 8 Size: 253 889 1629 43 Fragments arranged by size: 1629 1425 889 576 288 275 253 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2246 3622 1353 Size: 848 45 1376 3423 Fragments arranged by size: 3423 1376 848 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2945 4279 4653 4799 387 Size: 1064 138 1356 1334 374 146 1280 Fragments arranged by size: 1356 1334 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2092 2708 2756 3755 4352 5139 5407 Size: 393 460 616 48 999 597 787 268 Cuts at: 5407 1239 Size: 1524 Fragments arranged by size: 1524 999 787 616 597 460 393 268 48 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2505 2537 4333 4365 4407 Size: 32 135 32 933 32 1796 32 42 Cuts at: 4407 4439 1373 Size: 32 2626 Fragments arranged by size: 2626 1796 933 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 2744 3017 2744 Size: 273 5419 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2696 2888 10 Size: 54 1425 343 576 288 192 2814 Fragments arranged by size: 2814 1425 576 343 288 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2492 2793 2874 3650 4009 4168 Size: 648 1041 285 301 81 776 359 159 Cuts at: 4168 518 Size: 2042 Fragments arranged by size: 2042 1041 776 648 359 301 285 159 81 MspA1I CmG'CkG Cuts at: 1606 3108 3785 4933 5178 1606 Size: 1502 677 1148 245 2120 Fragments arranged by size: 2120 1502 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2385 2409 513 Size: 552 1320 24 3796 Fragments arranged by size: 3796 1320 552 24 NdeI CA'TA_TG Cuts at: 387 2601 387 Size: 2214 3478 NheI G'CTAG_C Cuts at: 1052 2254 1052 Size: 1202 4490 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NsiI A_TGCA'T Cuts at: 2377 2377 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 2781 3050 4595 1132 Size: 97 1552 269 1545 2229 Fragments arranged by size: 2229 1552 1545 269 97 PasI CC'CwG_GG Cuts at: 1348 2149 1348 Size: 801 4891 PciI A'CATG_T Cuts at: 2777 4591 2777 Size: 1814 3878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PstI C_TGCA'G Cuts at: 830 3044 830 Size: 2214 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SexAI A'CCwGG_T Cuts at: 2268 2268 Size: 5692 SfcI C'TryA_G Cuts at: 826 1045 3040 4175 4856 5047 826 Size: 219 1995 1135 681 191 1471 Fragments arranged by size: 1995 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3796 4697 4959 820 Size: 197 926 819 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2385 2409 3131 513 Size: 552 1320 24 722 3074 Fragments arranged by size: 3074 1320 722 552 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2308 3719 3736 3889 4074 4519 325 Size: 1255 728 1411 17 153 185 445 1498 Fragments arranged by size: 1498 1411 1255 728 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2484 2516 2883 2915 5652 5684 2484 Size: 32 367 32 2737 32 2492 Fragments arranged by size: 2737 2492 367 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI