(Linear) (Six-base) MAPSORT of: KIEE5477.seq Check: 3735 from: 1 to: 987 KIEE5477.orf With 182 enzymes: SgfI * December 5, 2014 13:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 915 987 Size: 915 72 Acc65I G'GTAC_C Cuts at: 0 735 987 Size: 735 252 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 751 987 Size: 751 236 AflII C'TTAA_G Cuts at: 0 757 987 Size: 757 230 AflIII A'CryG_T Cuts at: 0 772 987 Size: 772 215 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 149 987 Size: 149 838 ApoI r'AATT_y Cuts at: 0 818 987 Size: 818 169 BanI G'GyrC_C Cuts at: 0 735 987 Size: 735 252 BbsI GAAGACnn'nnnn_ Cuts at: 0 795 943 987 Size: 795 148 44 Fragments arranged by size: 795 148 44 BfrBI ATG'CAT Cuts at: 0 370 987 Size: 370 617 BmtI G_CTAG'C Cuts at: 0 253 987 Size: 253 734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 87 703 987 Size: 87 616 284 Fragments arranged by size: 616 284 87 Bpu10I CC'TnA_GC Cuts at: 0 52 987 Size: 52 935 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 878 908 987 Size: 878 30 79 Fragments arranged by size: 878 79 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 267 366 886 987 Size: 267 99 520 101 Fragments arranged by size: 520 267 101 99 BseYI C'CCAG_C Cuts at: 0 77 987 Size: 77 910 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 269 987 Size: 269 718 BsiHKAI G_wGCw'C Cuts at: 0 308 965 987 Size: 308 657 22 Fragments arranged by size: 657 308 22 BsmI GAATG_Cn' Cuts at: 0 370 987 Size: 370 617 Bsp1286I G_dGCh'C Cuts at: 0 308 965 987 Size: 308 657 22 Fragments arranged by size: 657 308 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 915 987 Size: 915 72 BsrDI GCAATG_nn' Cuts at: 0 183 987 Size: 183 804 BssSI C'ACGA_G Cuts at: 0 322 987 Size: 322 665 BstXI CCAn_nnnn'nTGG Cuts at: 0 264 328 411 540 987 Size: 264 64 83 129 447 Fragments arranged by size: 447 264 129 83 64 BstYI r'GATC_y Cuts at: 0 947 987 Size: 947 40 BtgI C'CryG_G Cuts at: 0 380 404 987 Size: 380 24 583 Fragments arranged by size: 583 380 24 BtsI GCAGTG_nn' Cuts at: 0 136 281 308 731 987 Size: 136 145 27 423 256 Fragments arranged by size: 423 256 145 136 27 EaeI y'GGCC_r Cuts at: 0 401 689 881 987 Size: 401 288 192 106 Fragments arranged by size: 401 288 192 106 EarI CTCTTCn'nnn_ Cuts at: 0 196 241 987 Size: 196 45 746 Fragments arranged by size: 746 196 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 940 987 Size: 940 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 87 703 751 987 Size: 87 616 48 236 Fragments arranged by size: 616 236 87 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 500 532 987 Size: 500 32 455 Fragments arranged by size: 500 455 32 KpnI G_GTAC'C Cuts at: 0 739 987 Size: 739 248 MscI TGG'CCA Cuts at: 0 403 691 883 987 Size: 403 288 192 104 Fragments arranged by size: 403 288 192 104 MslI CAynn'nnrTG Cuts at: 0 202 487 788 869 987 Size: 202 285 301 81 118 Fragments arranged by size: 301 285 202 118 81 NcoI C'CATG_G Cuts at: 0 380 404 987 Size: 380 24 583 Fragments arranged by size: 583 380 24 NdeI CA'TA_TG Cuts at: 0 596 987 Size: 596 391 NheI G'CTAG_C Cuts at: 0 249 987 Size: 249 738 NsiI A_TGCA'T Cuts at: 0 372 987 Size: 372 615 NspI r_CATG'y Cuts at: 0 776 987 Size: 776 211 PasI CC'CwG_GG Cuts at: 0 144 987 Size: 144 843 PciI A'CATG_T Cuts at: 0 772 987 Size: 772 215 SexAI A'CCwGG_T Cuts at: 0 263 987 Size: 263 724 SmlI C'TyrA_G Cuts at: 0 757 987 Size: 757 230 StyI C'CwwG_G Cuts at: 0 380 404 987 Size: 380 24 583 Fragments arranged by size: 583 380 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 303 987 Size: 303 684 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 479 511 878 910 987 Size: 479 32 367 32 77 Fragments arranged by size: 479 367 77 32 32 Enzymes that do cut: AarI Acc65I AcuI AflII AflIII AlfI ApoI BanI BbsI BfrBI BmtI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI Hin4I KpnI MscI MslI NcoI NdeI NheI NsiI NspI PasI PciI SexAI SmlI StyI TaqII TstI Enzymes that do not cut: AatII AccI AclI AfeI AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NotI NruI PacI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI