################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbvI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI TseI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AciI 792 CCGC CCGC(-3/-1) AflIII 548 ACATGT A^C[GA][CT]GT AhdI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 225 GTGCTC G[AT]GC[AT]^C AspI 910 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 923 GGATCC G^GATCC BbsI 67 GAAGAC GAAGAC(2/6) BbvCI 692 CCTCAGC CCTCAGC(-5/-2) BciVI 942 GGATAC GTATCC(6/5) BcnI 672 CCGGG CC^[GC]GG BfaI 230 CTAG C^TAG BmyI 225 GTGCTC G[AGT]GC[ACT]^C BpmI 764 CTCCAG CTGGAG(16/14) BpuAI 67 GAAGAC GAAGAC(2/6) BsiHKAI 225 GTGCTC G[AT]GC[AT]^C BsmAI 908 GAGAC GTCTC(1/5) BsmI 292 GAATGC GAATGC(1/-1) Bsp1286I 225 GTGCTC G[AGT]GC[ACT]^C BspLU11I 548 ACATGT A^CATGT BspMI 724 ACCTGC ACCTGC(4/8) BsrI 63 ACTGG ACTGG(1/-1) BstXI 789 CCACCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 923 GGATCC [GA]^GATC[CT] Cac8I 500 GCCTGC GC[ACGT]^[ACGT]GC CfoI 498 GCGC GCG^C Cfr13I 360 GGCCC G^G[ACGT]CC Eam1105I 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 497 AGCGCC [GA]GCGC^[CT] HapII 672 CCGG C^CGG HhaI 498 GCGC GCG^C HinP1I 498 GCGC G^CGC HpaII 672 CCGG C^CGG MaeI 230 CTAG C^TAG MaeIII 542 GTGAC ^GT[ACGT]AC MflI 923 GGATCC [GA]^GATC[CT] MseI 719 TTAA T^TAA MspI 672 CCGG C^CGG NciI 672 CCGGG CC^[GC]GG NlaIV 923 GGATCC GG[ACGT]^[ACGT]CC NspI 548 ACATGT [GA]CATG^[CT] PflFI 910 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 510 CTGCAG CTGCA^G Sau96I 360 GGCCC G^G[ACGT]CC SmlI 271 CTCAAG C^T[CT][GA]AG SspI 841 AATATT AAT^ATT StuI 713 AGGCCT AGG^CCT TfiI 666 GATTC G^A[AT]TC Tru9I 719 TTAA T^TAA Tsp45I 542 GTGAC ^GT[GC]AC Tth111I 910 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 923 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 183 GTAC GT^AC 233 GTAC AluI 33 AGCT AG^CT 768 AGCT BalI 194 TGGCCA TGG^CCA 377 TGGCCA BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG 362 CCCTGG BseRI 139 CTCCTC GAGGAG(10/8) 690 CTCCTC BsgI 169 CTGCAC GTGCAG(16/14) 726 CTGCAC BsmFI 439 GGGAC GGGAC(10/14) 55 GTCCC BstNI 58 CCAGG CC^[AT]GG 363 CCTGG DpnII 367 GATC ^GATC 924 GATC EaeI 194 TGGCCA [CT]^GGCC[GA] 377 TGGCCA EcoRII 58 CCAGG ^CC[AT]GG 363 CCTGG MboI 367 GATC ^GATC 924 GATC MluNI 194 TGGCCA TGG^CCA 377 TGGCCA MscI 194 TGGCCA TGG^CCA 377 TGGCCA MslI 327 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 736 CACATTTGTG MvaI 58 CCAGG CC^[AT]GG 363 CCTGG NdeII 367 GATC ^GATC 924 GATC RsaI 183 GTAC GT^AC 233 GTAC Sau3AI 367 GATC ^GATC 924 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AlwI BsiYI BslI BssKI BstF5I DdeI FokI HaeIII HinfI HphI MboII MnlI NlaIII PleI ScrFI SfaNI SfcI Tsp509I TspRI