(Circular) (Six-base) MAPSORT of: pFN21AE6069.seq Check: 3700 from: 1 to: 5668 pFN21AE6069 5668 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 5115 5383 1632 Size: 2099 1384 268 1917 Fragments arranged by size: 2099 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 2553 4567 5544 1203 Size: 1350 2014 977 1327 Fragments arranged by size: 2014 1350 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 2554 4397 2554 Size: 1843 3825 AleI CACnn'nnGTG Cuts at: 1166 2745 1166 Size: 1579 4089 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2799 2831 2799 Size: 32 5636 AlwNI CAG_nnn'CTG Cuts at: 3411 4983 3411 Size: 1572 4096 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2928 2998 3365 2928 Size: 70 367 5231 Fragments arranged by size: 5231 367 70 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2989 4444 618 Size: 325 217 627 1202 1455 1842 Fragments arranged by size: 1842 1455 1202 627 325 217 BanII G_rGCy'C Cuts at: 1391 2987 1391 Size: 1596 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 928 Size: 971 180 4517 Fragments arranged by size: 4517 971 180 BbvCI CC'TCA_GC Cuts at: 2698 2698 Size: 5668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3859 3893 1903 Size: 34 50 34 1838 34 3678 Fragments arranged by size: 3678 1838 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2941 3560 4770 2941 Size: 619 1210 3839 Fragments arranged by size: 3839 1210 619 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4279 136 Size: 107 122 71 631 605 2607 1525 Fragments arranged by size: 2607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 3729 4885 1790 Size: 1939 1156 2573 Fragments arranged by size: 2573 1939 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3399 4357 442 Size: 416 53 83 399 24 1982 958 1753 Fragments arranged by size: 1982 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2754 4328 1239 Size: 1515 1574 2579 Fragments arranged by size: 2579 1574 1515 Bpu10I CC'TnA_GC Cuts at: 2698 2698 Size: 5668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 3793 4658 4956 2261 Size: 1532 865 298 2973 Fragments arranged by size: 2973 1532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5443 Fragments arranged by size: 5443 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2686 1186 Size: 949 551 4168 Fragments arranged by size: 4168 949 551 BseYI C'CCAG_C Cuts at: 4871 5367 4871 Size: 496 5172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2716 1163 Size: 573 423 557 4115 Fragments arranged by size: 4115 573 557 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2234 3729 3814 4885 2234 Size: 1495 85 1071 3017 Fragments arranged by size: 3017 1495 1071 85 BsmI GAATG_Cn' Cuts at: 2303 3179 3272 2303 Size: 876 93 4699 Fragments arranged by size: 4699 876 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2987 3729 3814 4885 1391 Size: 399 444 753 742 85 1071 2174 Fragments arranged by size: 2174 1071 753 742 444 399 85 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 3023 844 Size: 1894 285 3489 Fragments arranged by size: 3489 1894 285 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2928 2998 3365 3750 3767 5219 5305 5317 Size: 956 70 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 956 385 367 346 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2688 2733 3184 3978 3998 2688 Size: 45 451 794 20 4358 Fragments arranged by size: 4358 794 451 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2382 3115 Size: 54 1425 50 275 18 369 183 733 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 733 369 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3598 1353 Size: 2245 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2754 3731 4328 5115 5383 1239 Size: 393 1122 977 597 787 268 1524 Fragments arranged by size: 1524 1122 977 787 597 393 268 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2506 4815 1678 Size: 828 2309 2531 Fragments arranged by size: 2531 2309 828 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 2904 2936 2939 Size: 32 135 32 968 32 332 32 3 Cuts at: 2939 2971 4309 4341 4383 4415 1373 Size: 32 1338 32 42 32 2626 Fragments arranged by size: 2626 1338 968 332 135 42 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2384 10 Size: 54 1425 343 369 183 3294 Fragments arranged by size: 3294 1425 369 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2745 3626 3985 4144 518 Size: 648 1170 409 881 359 159 2042 Fragments arranged by size: 2042 1170 881 648 409 359 159 MspA1I CmG'CkG Cuts at: 1606 3084 3761 4909 5154 1606 Size: 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5116 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2557 3026 4571 1132 Size: 97 1328 469 1545 2229 Fragments arranged by size: 2229 1545 1328 469 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 2553 4567 2553 Size: 2014 3654 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 2519 3020 830 Size: 1689 501 3478 Fragments arranged by size: 3478 1689 501 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2515 2559 2959 3016 4151 4832 5023 Size: 219 1470 44 400 57 1135 681 191 Cuts at: 5023 826 Size: 1471 Fragments arranged by size: 1471 1470 1135 681 400 219 191 57 44 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3772 4673 4935 820 Size: 197 926 333 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 333 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 2848 3592 5 Size: 47 2796 744 2081 Fragments arranged by size: 2796 2081 744 47 StuI AGG'CCT Cuts at: 1517 2720 1517 Size: 1203 4465 StyI C'CwwG_G Cuts at: 513 1065 3107 513 Size: 552 2042 3074 Fragments arranged by size: 3074 2042 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2881 3695 3712 3865 4050 4495 325 Size: 1255 1301 814 17 153 185 445 1498 Fragments arranged by size: 1498 1301 1255 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2237 3914 Size: 274 80 33 51 1443 210 50 1677 Cuts at: 3914 5626 96 Size: 1712 138 Fragments arranged by size: 1712 1677 1443 274 210 138 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 2918 1316 Size: 1602 4066 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI